##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931129_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2427938 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.099266126235513 31.0 28.0 33.0 25.0 34.0 2 28.84528435240109 31.0 28.0 33.0 22.0 34.0 3 28.87984660234322 31.0 28.0 34.0 22.0 34.0 4 32.07657609049325 35.0 32.0 37.0 20.0 37.0 5 32.174261451486814 35.0 32.0 37.0 22.0 37.0 6 32.44302243302753 35.0 32.0 37.0 25.0 37.0 7 32.181303229324634 35.0 32.0 37.0 22.0 37.0 8 32.32135499341416 35.0 32.0 37.0 23.0 37.0 9 33.41509544312911 37.0 32.0 39.0 22.0 39.0 10-11 33.36015746695344 37.0 32.0 39.0 21.0 39.0 12-13 33.460067349331 37.0 32.0 39.0 23.0 39.0 14-15 34.23727994701677 38.0 32.0 40.0 22.5 41.0 16-17 34.195959081327445 38.0 32.0 40.0 23.0 41.0 18-19 34.241700982479784 38.0 32.0 40.0 23.0 41.0 20-21 34.40458693755771 38.0 32.5 40.0 24.0 41.0 22-23 34.33345929756031 38.0 32.5 40.0 24.0 41.0 24-25 34.23201416181138 38.0 32.5 40.0 23.0 41.0 26-27 33.879756196410284 38.0 32.0 40.0 20.0 41.0 28-29 33.84436278850613 38.0 32.0 40.0 21.5 41.0 30-31 33.623532396626274 38.0 32.0 40.0 20.0 41.0 32-33 33.40452474486581 37.0 31.0 40.0 18.5 41.0 34-35 33.38324104651767 37.0 31.0 40.0 19.5 41.0 36-37 33.05693555601502 37.0 31.0 40.0 17.0 41.0 38-39 32.897479672050935 37.0 31.0 40.0 16.5 41.0 40-41 32.77759069630279 37.0 30.5 40.0 16.0 41.0 42-43 32.97395917852927 37.0 31.0 40.0 15.5 41.0 44-45 33.058658417142446 37.0 31.0 40.0 16.0 41.0 46-47 32.84747715963093 37.0 31.0 40.0 15.0 41.0 48-49 32.629966251197516 37.0 30.5 40.0 13.5 41.0 50-51 32.13990616729093 36.5 30.0 39.5 9.0 40.5 52-53 32.098683739041114 37.0 30.0 39.5 9.0 40.5 54-55 32.1056060739607 37.0 30.0 40.0 8.0 41.0 56-57 31.88133263699485 36.5 30.0 40.0 7.5 41.0 58-59 31.740699309455184 36.0 30.0 40.0 7.0 41.0 60-61 31.592439963458702 36.0 30.0 40.0 6.0 41.0 62-63 31.44319027092125 36.0 30.0 39.5 2.0 41.0 64-65 31.108595441893492 35.0 29.0 39.0 2.0 41.0 66-67 30.916952780507575 35.0 29.0 39.0 2.0 40.0 68-69 30.73236631248409 35.0 29.0 39.0 2.0 40.0 70-71 30.434841210937016 35.0 29.0 38.5 2.0 40.0 72-73 30.108856362889 34.5 29.0 38.0 2.0 40.0 74-75 29.708244815147665 34.0 29.0 37.0 2.0 40.0 76-77 29.38321448076516 34.0 29.0 37.0 2.0 39.0 78-79 28.95424347738698 34.0 28.0 36.0 2.0 39.0 80-81 28.54322247932196 34.0 28.0 36.0 2.0 38.5 82-83 28.19769615204342 33.5 27.5 35.5 2.0 37.0 84-85 27.843216342427198 33.0 27.0 35.0 2.0 37.0 86-87 27.50002821324103 33.0 26.0 35.0 2.0 36.5 88-89 27.152247503848947 33.0 26.0 35.0 2.0 36.0 90-91 26.774237851213663 33.0 26.0 35.0 2.0 36.0 92-93 26.41833419963772 33.0 25.0 35.0 2.0 35.5 94-95 26.1198463469825 32.0 25.0 35.0 2.0 35.0 96-97 25.74759013615669 32.0 24.0 35.0 2.0 35.0 98-99 25.37784449191042 32.0 21.5 35.0 2.0 35.0 100-101 24.785777272731018 32.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.968080623821468 1101 2 5.614204519744607 1101 3 5.232306164359667 1101 4 6.6429917349824485 1101 5 6.115784990263624 1101 6 5.50243716956366 1101 7 6.150144534771641 1101 8 5.602521491482786 1101 9 6.20937163198866 1101 10-11 6.244524768065855 1101 12-13 6.00506945341964 1101 14-15 6.241452919550735 1101 16-17 6.22556921502559 1101 18-19 6.195973142726082 1101 20-21 5.603720545585041 1101 22-23 5.690024879979216 1101 24-25 5.616174104835565 1101 26-27 5.720935606276058 1101 28-29 5.6362118359985445 1101 30-31 5.73009518449528 1101 32-33 5.537517264381467 1101 34-35 5.447862934461401 1101 36-37 5.6262848877276745 1101 38-39 5.494839047056907 1101 40-41 5.614056452476927 1101 42-43 5.385376085309151 1101 44-45 5.422125351501457 1101 46-47 5.313967458887735 1101 48-49 5.224704457174415 1101 50-51 5.2360338320424695 1101 52-53 5.087795245973538 1101 54-55 5.266212379666772 1101 56-57 5.317744275375379 1101 58-59 5.1202594959063 1101 60-61 5.058745413868106 1101 62-63 4.880949535612679 1101 64-65 4.857622210445218 1101 66-67 4.849216231621256 1101 68-69 4.830912958678908 1101 70-71 4.620807160110136 1101 72-73 4.442697143187246 1101 74-75 4.58513209818431 1101 76-77 4.4167481375104565 1101 78-79 4.35511466538545 1101 80-81 4.422314339530317 1101 82-83 4.195970070259577 1101 84-85 3.961306044139942 1101 86-87 3.791137727219855 1101 88-89 3.7697502235775 1101 90-91 3.840868734700546 1101 92-93 3.7422269116454494 1101 94-95 3.657032573851019 1101 96-97 3.5250408647762974 1101 98-99 3.4100323198278986 1101 100-101 3.4182720251864893 1102 1 5.253974693570182 1102 2 5.7511008230602485 1102 3 5.482643946099351 1102 4 6.908191663568235 1102 5 6.507161099712146 1102 6 5.926873315643181 1102 7 6.453946842249785 1102 8 6.0101555710447165 1102 9 6.70872927861565 1102 10-11 6.653179995973328 1102 12-13 6.628626280780775 1102 14-15 6.954919867379655 1102 16-17 6.903305709062135 1102 18-19 6.91622793665487 1102 20-21 6.173556405258616 1102 22-23 6.327174004593278 1102 24-25 6.167301687826502 1102 26-27 6.309927829368064 1102 28-29 6.232529759093431 1102 30-31 6.442415010304526 1102 32-33 6.2031132172430965 1102 34-35 6.218727183781905 1102 36-37 6.261892364246503 1102 38-39 6.081417669486719 1102 40-41 6.3241940247182775 1102 42-43 6.287035841526997 1102 44-45 6.170416341949185 1102 46-47 6.205961201404538 1102 48-49 5.8635074700552 1102 50-51 5.911312403658311 1102 52-53 5.642352958521336 1102 54-55 5.99841257418311 1102 56-57 5.8212096126389845 1102 58-59 5.954914741292786 1102 60-61 5.802990514441046 1102 62-63 5.592170181811433 1102 64-65 5.681998678213308 1102 66-67 5.63736746495222 1102 68-69 5.675769075268853 1102 70-71 5.5481700404501275 1102 72-73 5.321929215660191 1102 74-75 5.381766516089797 1102 76-77 5.283659884774057 1102 78-79 5.173639787083012 1102 80-81 5.141865978124454 1102 82-83 5.013684291051852 1102 84-85 4.917510278803054 1102 86-87 4.935580465380639 1102 88-89 4.850176611379151 1102 90-91 5.00615855530355 1102 92-93 5.026026871943142 1102 94-95 4.904765984091966 1102 96-97 4.757516478445378 1102 98-99 4.543109086362097 1102 100-101 4.674948192939979 1103 1 4.977206449715958 1103 2 5.596827445574387 1103 3 5.220613396363348 1103 4 6.674945052982277 1103 5 6.261297698689429 1103 6 5.6075761350453455 1103 7 6.127340506214857 1103 8 5.575826548998375 1103 9 6.256405286488658 1103 10-11 6.208281879986981 1103 12-13 6.33904168274071 1103 14-15 6.479789593077125 1103 16-17 6.40442108530954 1103 18-19 6.345008148248233 1103 20-21 5.639859267464281 1103 22-23 5.83666002777716 1103 24-25 5.53736677045908 1103 26-27 5.761026292143981 1103 28-29 5.697185827327495 1103 30-31 5.962738435437185 1103 32-33 5.698494394146845 1103 34-35 5.6754973903088235 1103 36-37 5.559813401917143 1103 38-39 5.53358977001816 1103 40-41 5.570560189458018 1103 42-43 5.641940722901939 1103 44-45 5.34665798600961 1103 46-47 5.215059634765712 1103 48-49 5.037409146745691 1103 50-51 5.120110451737201 1103 52-53 4.801494296820692 1103 54-55 5.0479353962123135 1103 56-57 5.1153894766313535 1103 58-59 5.054926963621501 1103 60-61 4.911018986888319 1103 62-63 4.890149338937327 1103 64-65 4.932629421376543 1103 66-67 5.07043221134397 1103 68-69 5.055560519740176 1103 70-71 4.961429213155693 1103 72-73 4.6786148294721315 1103 74-75 4.614612477725888 1103 76-77 4.59777180580463 1103 78-79 4.586301308200007 1103 80-81 4.643034072673551 1103 82-83 4.633224954638084 1103 84-85 4.583062507450876 1103 86-87 4.624467249594666 1103 88-89 4.613451531109899 1103 90-91 4.512485537590305 1103 92-93 4.458951340487381 1103 94-95 4.350212228413781 1103 96-97 4.412182466544582 1103 98-99 4.187146542474558 1103 100-101 4.206252112554587 1104 1 4.588755668358999 1104 2 5.420849038017032 1104 3 5.03321876709229 1104 4 6.5299060340302155 1104 5 6.105145612789055 1104 6 5.664731565427321 1104 7 6.2070310927704355 1104 8 5.610675656158694 1104 9 6.365613368007949 1104 10-11 6.23222426102318 1104 12-13 6.255764090767467 1104 14-15 6.282274484234787 1104 16-17 6.202737109483682 1104 18-19 6.387583790251796 1104 20-21 5.671227962334122 1104 22-23 5.916091465904255 1104 24-25 5.554977128572872 1104 26-27 5.7760510334845065 1104 28-29 5.754746116009066 1104 30-31 5.993624038519588 1104 32-33 5.85205890743676 1104 34-35 5.725548242075927 1104 36-37 5.787175564290745 1104 38-39 5.548669274800183 1104 40-41 5.8894154379471395 1104 42-43 5.813332692234415 1104 44-45 5.613499700105329 1104 46-47 5.767227680297598 1104 48-49 5.501955685891776 1104 50-51 5.481674339159216 1104 52-53 5.280127753371772 1104 54-55 5.435524631284384 1104 56-57 5.461782912841716 1104 58-59 5.269634859336268 1104 60-61 5.141122255092274 1104 62-63 4.942008362192233 1104 64-65 5.23346749761507 1104 66-67 5.167453471294294 1104 68-69 5.012652385413279 1104 70-71 4.85974425941124 1104 72-73 4.744625312759503 1104 74-75 4.855358434032485 1104 76-77 4.697789408339393 1104 78-79 4.716290159927986 1104 80-81 4.803073385001191 1104 82-83 4.5280126485104475 1104 84-85 4.500570184111869 1104 86-87 4.406544935448096 1104 88-89 4.4545348693328215 1104 90-91 4.464159734230172 1104 92-93 4.410411372954279 1104 94-95 4.341539068367624 1104 96-97 4.298162047604258 1104 98-99 4.201394816448229 1104 100-101 4.249628669889951 1105 1 4.318739984581686 1105 2 5.024266450547543 1105 3 4.729503734704245 1105 4 6.139811214756495 1105 5 3.560426709800794 1105 6 4.8551288121941845 1105 7 5.466217078421906 1105 8 0.8197092941872732 1105 9 0.4397322327165476 1105 10-11 2.064125916754744 1105 12-13 5.260720763085377 1105 14-15 3.436798242572177 1105 16-17 5.506313718250087 1105 18-19 5.944092376416869 1105 20-21 2.9662301983382307 1105 22-23 5.109818317819094 1105 24-25 5.322295910191016 1105 26-27 5.593980974441294 1105 28-29 3.0034709586950683 1105 30-31 5.283250249386111 1105 32-33 5.41355518877597 1105 34-35 5.3976197796938585 1105 36-37 5.553763403875362 1105 38-39 5.358801230791265 1105 40-41 5.5026887756164165 1105 42-43 2.8808294470803766 1105 44-45 4.898438103407905 1105 46-47 5.143115754912106 1105 48-49 5.102768085600413 1105 50-51 5.3291327780564295 1105 52-53 5.232295316707834 1105 54-55 5.462709905768623 1105 56-57 5.431513404046012 1105 58-59 5.436066042567962 1105 60-61 5.369160295282295 1105 62-63 5.065063424133626 1105 64-65 5.11359853743409 1105 66-67 5.110489611107592 1105 68-69 5.221039532516951 1105 70-71 5.027606122433539 1105 72-73 5.011636768538779 1105 74-75 5.0250120030669585 1105 76-77 4.888669686420297 1105 78-79 4.873766622923249 1105 80-81 4.914134031056708 1105 82-83 4.649705516791208 1105 84-85 4.645588884000208 1105 86-87 4.702392589639402 1105 88-89 4.761519184648407 1105 90-91 4.697418685276649 1105 92-93 4.628904895799163 1105 94-95 4.450230547876664 1105 96-97 4.378113481413546 1105 98-99 4.265768150216971 1105 100-101 4.516404279393832 1106 1 4.180638862380469 1106 2 4.485031849740828 1106 3 4.618624167109235 1106 4 6.282935423267734 1106 5 6.066580566526223 1106 6 5.244839791722288 1106 7 5.894933473117575 1106 8 5.445868308600101 1106 9 6.100958873061224 1106 10-11 5.926967709544247 1106 12-13 6.2221476478138875 1106 14-15 6.025067151296877 1106 16-17 5.995327662195404 1106 18-19 6.195064596449633 1106 20-21 5.496781513083683 1106 22-23 5.611450810764662 1106 24-25 5.621406209114568 1106 26-27 5.797547842686235 1106 28-29 5.751303193998787 1106 30-31 5.905532004632821 1106 32-33 5.584530201882398 1106 34-35 5.31334312716233 1106 36-37 5.3556175642010295 1106 38-39 5.343643248320127 1106 40-41 5.440660106796937 1106 42-43 2.4073785014976146 1106 44-45 4.683021387731081 1106 46-47 4.947838575698231 1106 48-49 4.719088386880504 1106 50-51 5.071074093598167 1106 52-53 4.852490755064778 1106 54-55 5.161805228805182 1106 56-57 5.146983374057857 1106 58-59 5.087425574204676 1106 60-61 4.931112568888718 1106 62-63 4.840906328089488 1106 64-65 4.9159450224660866 1106 66-67 5.121233748987613 1106 68-69 5.044574573465191 1106 70-71 4.945388906994591 1106 72-73 4.757019001275786 1106 74-75 4.685996826288839 1106 76-77 4.633939549478843 1106 78-79 4.674802970472157 1106 80-81 4.709741164833616 1106 82-83 4.770358720115247 1106 84-85 4.788982294690765 1106 86-87 4.71771538770907 1106 88-89 4.795886319023545 1106 90-91 4.687354121216433 1106 92-93 4.679598778563637 1106 94-95 4.633073193543943 1106 96-97 4.681774752314606 1106 98-99 4.594632666742388 1106 100-101 4.640264869043612 1107 1 3.1902037499195366 1107 2 0.1992125745354265 1107 3 1.4361246642894265 1107 4 3.18952917835718 1107 5 3.470622334572635 1107 6 4.343516146650838 1107 7 5.120233438499099 1107 8 4.55230967693819 1107 9 5.022237039444313 1107 10-11 2.9024373913734784 1107 12-13 5.110062465911415 1107 14-15 3.844724551179521 1107 16-17 2.258175473497726 1107 18-19 3.2441956155012477 1107 20-21 2.8675122083748263 1107 22-23 4.482073579080332 1107 24-25 4.363296136284831 1107 26-27 3.6550260260921164 1107 28-29 4.483829560916952 1107 30-31 3.974637493583966 1107 32-33 4.4150655155887755 1107 34-35 4.8220752890327425 1107 36-37 4.996549938692699 1107 38-39 5.064257341557571 1107 40-41 3.9951972954659816 1107 42-43 4.282428424117558 1107 44-45 4.5876624318176695 1107 46-47 4.593862545921436 1107 48-49 2.8092399429521926 1107 50-51 4.108165168006611 1107 52-53 4.370315483293403 1107 54-55 4.985469532740641 1107 56-57 3.9242754974960405 1107 58-59 3.1403175670256367 1107 60-61 3.857328573425942 1107 62-63 4.6109333804206685 1107 64-65 4.74210433209354 1107 66-67 4.7895665830947465 1107 68-69 4.791113033115028 1107 70-71 4.472555405479319 1107 72-73 4.358238647709058 1107 74-75 4.580473911397085 1107 76-77 4.532873865223866 1107 78-79 4.575276089596532 1107 80-81 4.563554229992459 1107 82-83 4.313073045697482 1107 84-85 4.315191363257437 1107 86-87 4.318169212968442 1107 88-89 4.331334755095174 1107 90-91 4.353716034840485 1107 92-93 4.374748243614121 1107 94-95 4.346293521707423 1107 96-97 4.37105994632946 1107 98-99 4.3293044787125226 1107 100-101 4.469553037955571 1108 1 -2.2774234975990915 1108 2 -1.4990118920133888 1108 3 -2.2189475878006704 1108 4 -4.137527784914287 1108 5 -9.237695873899149 1108 6 -7.567919644821533 1108 7 -6.311023439285574 1108 8 -6.475630864635669 1108 9 -7.679667511760773 1108 10-11 -3.6748067084985756 1108 12-13 -2.121844888315355 1108 14-15 -7.076255833396488 1108 16-17 -5.831391719191938 1108 18-19 -4.564964251242451 1108 20-21 -5.0671693318479925 1108 22-23 -3.912036191258043 1108 24-25 -8.744047870009975 1108 26-27 -9.470493946958776 1108 28-29 -6.6188438165344365 1108 30-31 -6.564730552112092 1108 32-33 -2.97709287912107 1108 34-35 -1.2598758646451564 1108 36-37 -4.951295421858941 1108 38-39 -6.108407988393516 1108 40-41 -3.6589567253844955 1108 42-43 -4.259238779526445 1108 44-45 -1.7822771832913027 1108 46-47 -2.4329423780891126 1108 48-49 -1.3346106463267304 1108 50-51 -0.6685779141144117 1108 52-53 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-4.421427112623881 2313 56-57 -3.450464260275016 2313 58-59 -3.641943417459398 2313 60-61 -2.0208780634297874 2313 62-63 -4.400641741327437 2313 64-65 -5.144984485184732 2313 66-67 -5.847214469460603 2313 68-69 -7.510743059721296 2313 70-71 -5.684721419837047 2313 72-73 -4.677066039366636 2313 74-75 -5.838796686209591 2313 76-77 -4.156614159107651 2313 78-79 -5.166445944547249 2313 80-81 -7.446950504153133 2313 82-83 -6.398424682274708 2313 84-85 -6.414543687523743 2313 86-87 -5.8596758003378575 2313 88-89 -7.36526127655028 2313 90-91 -7.4186082224508265 2313 92-93 -6.403364706039191 2313 94-95 -6.865149894843077 2313 96-97 -6.912619924047281 2313 98-99 -7.016792793043727 2313 100-101 -6.854894343328713 2314 1 -14.08209779379881 2314 2 -15.345817628649636 2314 3 -13.813387203426727 2314 4 -25.18402930599208 2314 5 -19.658442892103793 2314 6 -18.68279723427539 2314 7 -17.841432923084888 2314 8 -13.564674275712978 2314 9 -15.785804939932097 2314 10-11 -14.592475354980783 2314 12-13 -16.07192697822795 2314 14-15 -18.223408037752083 2314 16-17 -18.202697673125012 2314 18-19 -16.821114958664346 2314 20-21 -16.799749369512774 2314 22-23 -17.314337123219993 2314 24-25 -19.126544610557563 2314 26-27 -17.124376316942843 2314 28-29 -18.94762598853695 2314 30-31 -18.44802404769372 2314 32-33 -15.519791705718198 2314 34-35 -18.01701647983662 2314 36-37 -16.58057496215543 2314 38-39 -16.650002222784362 2314 40-41 -18.55951928272671 2314 42-43 -18.582099612980496 2314 44-45 -15.634636061073909 2314 46-47 -14.5854726789245 2314 48-49 -14.192872754588839 2314 50-51 -14.347407167096206 2314 52-53 -14.899247943395231 2314 54-55 -14.930228496425267 2314 56-57 -13.841032025842786 2314 58-59 -13.172468447984432 2314 60-61 -13.195481238032961 2314 62-63 -15.475122815808511 2314 64-65 -13.723840814041061 2314 66-67 -14.606880729126866 2314 68-69 -17.21042763440587 2314 70-71 -17.22806696318259 2314 72-73 -15.424267911568506 2314 74-75 -14.09703844445135 2314 76-77 -14.186884814378306 2314 78-79 -13.435066213167518 2314 80-81 -13.558570365772994 2314 82-83 -13.886062044912073 2314 84-85 -13.567016214996269 2314 86-87 -14.073351014013072 2314 88-89 -12.910183946472952 2314 90-91 -12.1411356949783 2314 92-93 -12.431905609580095 2314 94-95 -12.011873541566723 2314 96-97 -11.863983375410733 2314 98-99 -11.61162388787482 2314 100-101 -10.897324135758506 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67599.0 3 11577.0 4 7733.0 5 6392.0 6 6545.0 7 7557.0 8 8861.0 9 10074.0 10 11414.0 11 12680.0 12 13820.0 13 15025.0 14 16994.0 15 18786.0 16 20386.0 17 21544.0 18 20147.0 19 18578.0 20 18672.0 21 19860.0 22 22975.0 23 27748.0 24 34099.0 25 41903.0 26 50555.0 27 57891.0 28 65542.0 29 73957.0 30 85507.0 31 96087.0 32 111335.0 33 134187.0 34 164016.0 35 200725.0 36 249026.0 37 306671.0 38 291716.0 39 79625.0 40 129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87521090653035 20.27022405638513 14.509874854914848 23.34469018216968 2 14.370833269649493 20.832381518109823 41.61754749334211 23.17923771889857 3 17.712766965219046 25.96969115356323 31.114550701047555 25.20299118017017 4 11.366387628295369 17.98471765697255 40.75660192413017 29.892292790601903 5 12.374803266702191 37.79563696148778 36.413484854117875 13.416074917692159 6 29.100696630390104 38.572717831503425 19.126476531137694 13.200109006968777 7 25.595381919389755 32.751675085042784 23.633151221523555 18.019791774043913 8 24.103143913435378 36.11603855196739 21.674513461603485 18.106304072993748 9 24.83456447027438 16.721018115546133 21.95809530092588 36.48632211325361 10-11 23.07106246688434 27.074765027058678 30.22423052562041 19.62994198043657 12-13 24.190878752885407 24.639827455727524 29.69161509646782 21.47767869491925 14-15 21.39105149030479 26.38879151569239 27.501478468792968 24.718678525209853 16-17 20.871130465442107 29.407009003269135 28.168696087861516 21.553164443427242 18-19 20.757790928080073 28.546871875223793 29.924399635361514 20.770937561334616 20-21 21.641693504861387 27.78859185342078 29.967505546651868 20.602209095065966 22-23 21.26766411120979 27.700402160800003 29.67492345586168 21.357010272128534 24-25 21.15689664519845 27.79360826642166 29.776912076918656 21.272583011461236 26-27 21.111651815859904 27.962669909244152 29.672204969200465 21.25347330569548 28-29 21.072667306216978 28.052115721907363 29.700967964137348 21.17424900773831 30-31 21.323088800477404 27.84745211201213 29.669107170032 21.16035191747846 32-33 21.22989343152789 27.953948830476268 29.541694136486974 21.274463601508874 34-35 21.470527512268387 27.999559445652277 29.509757474419075 21.02015556766026 36-37 21.300547660666513 27.813762376809347 29.500258610662677 21.385431351861463 38-39 21.227791889948985 28.042630167766426 29.57101336662513 21.15856457565945 40-41 21.465786817435134 27.835567591816808 29.427126283983927 21.27151930676413 42-43 21.361350819831657 27.92820950316538 29.377189360073412 21.333250316929554 44-45 21.47960040771358 28.094206983692487 29.159600902411547 21.266591706182385 46-47 21.398315347782376 28.052429816979647 29.01390148311483 21.535353352123153 48-49 21.34293282759047 28.140920850555045 29.111143178140363 21.40500314371412 50-51 21.338494469401613 28.245842389540044 28.960321705318503 21.455341435739836 52-53 21.372098071142208 28.327904794120563 28.8406219228057 21.45937521193153 54-55 21.396006352056556 28.24146904632593 28.79963938630391 21.562885215313603 56-57 21.388593122267203 28.316578122646362 28.670223855786105 21.624604899300333 58-59 21.367102398765343 28.336983607451767 28.78150557171394 21.514408422068957 60-61 21.540558007686514 28.30306922174197 28.64471500054356 21.51165777002796 62-63 21.348362272842223 28.330274496300976 28.671551744731538 21.649811486125266 64-65 21.283513005686306 28.215671075620545 28.763481604554975 21.73733431413817 66-67 21.364425285983415 28.259885548972008 28.643029599602627 21.73265956544195 68-69 21.418710032958007 28.247694133869977 28.611974440862987 21.72162139230903 70-71 21.409813594910577 27.99019167705271 28.621365125468607 21.978629602568105 72-73 21.40917519310625 28.040728387627688 28.65602416536172 21.894072253904344 74-75 21.310984876879065 28.20568317642378 28.54483928337544 21.938492663321714 76-77 21.37857309371162 28.159471123232965 28.48262599786321 21.97932978519221 78-79 21.402070398832258 28.19752810821364 28.40482335216138 21.99557814079272 80-81 21.390640947174106 28.136159160571644 28.452785861912457 22.020414030341794 82-83 21.444740351689376 28.11653345349016 28.546054306164326 21.892671888656135 84-85 21.600633953585305 28.05308455158245 28.38455924327557 21.96172225155667 86-87 21.29834040243202 28.058809574214827 28.580301473925612 22.06254854942754 88-89 21.42945989559865 27.961751906350162 28.5281790556431 22.080609142408083 90-91 21.446387840216676 28.022544233007597 28.48845398852854 22.04261393824719 92-93 21.48755445979263 27.971966335219435 28.49108997017222 22.049389234815717 94-95 21.318789853777158 27.94171432713685 28.49595006132776 22.24354575775823 96-97 21.3248237805084 27.882322365727624 28.602707317896915 22.19014653586706 98-99 21.33126957937147 27.86765971783464 28.44137700386089 22.359693698933004 100-101 21.364157569097728 27.82214784726793 28.422575041043057 22.391119542591284 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 183.0 1 130.0 2 103.5 3 121.0 4 112.5 5 121.5 6 183.5 7 341.0 8 600.5 9 829.5 10 906.5 11 970.5 12 1082.5 13 1195.5 14 1397.0 15 1601.0 16 1770.0 17 2021.0 18 2335.5 19 2662.0 20 3125.0 21 3854.0 22 4878.5 23 6460.0 24 8615.5 25 11523.0 26 15582.0 27 20712.0 28 26809.5 29 33381.0 30 39752.0 31 46226.0 32 53191.0 33 60119.5 34 66563.5 35 72209.0 36 78413.5 37 84363.0 38 87952.5 39 90037.0 40 91448.0 41 92649.0 42 93639.0 43 93559.0 44 93404.5 45 93733.5 46 92472.5 47 90149.0 48 87885.5 49 84960.5 50 80785.0 51 76049.0 52 71083.0 53 65634.5 54 60197.0 55 54027.5 56 47936.0 57 42250.0 58 36740.5 59 31729.0 60 26314.5 61 21099.0 62 16461.0 63 12564.0 64 9360.5 65 6782.5 66 5063.5 67 3903.5 68 3033.5 69 2395.0 70 1891.0 71 1449.5 72 1151.0 73 919.5 74 679.5 75 520.5 76 406.5 77 300.0 78 219.5 79 155.5 80 119.0 81 98.0 82 91.0 83 67.5 84 46.5 85 41.0 86 29.5 87 20.5 88 21.5 89 15.0 90 8.0 91 7.5 92 8.5 93 6.5 94 2.5 95 2.5 96 4.0 97 2.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037686300062027944 2 0.29345889392562746 3 0.0 4 0.5837051852230164 5 0.29440619982882593 6 0.0990552477040188 7 0.1107112290346788 8 0.1130177129729013 9 0.11009342083694064 10-11 0.15113647877334596 12-13 0.24032738892014543 14-15 0.12899835168772844 16-17 0.12296442495648571 18-19 0.21683008379950394 20-21 0.10712794148779747 22-23 0.10461552148366227 24-25 0.09944652622925296 26-27 0.21310264100648368 28-29 0.11518004166498487 30-31 0.1303575297227524 32-33 0.10498620640230517 34-35 0.15319583943247314 36-37 0.14184876220068224 38-39 0.1366179861264991 40-41 0.1101346080501232 42-43 0.11180269018401623 44-45 0.09125027080592668 46-47 0.09611036196146688 48-49 0.10144410606860636 50-51 0.08713154948767225 52-53 0.0960279875351018 54-55 0.31382597084439556 56-57 0.3354080705520487 58-59 0.34947350385388753 60-61 0.1681467978177367 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2427938.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.40522406337074 #Duplication Level Percentage of deduplicated Percentage of total 1 88.49554762664144 71.15504335530801 2 7.9840683811124 12.839216142412324 3 1.8204804734128117 4.391284211032452 4 0.6496660344908187 2.089461722783833 5 0.3168497708199094 1.273818840860124 6 0.1875671932047622 0.9048829317939872 7 0.10997747334187097 0.6189934370183562 8 0.08523942171553535 0.5482955841655814 9 0.05545524216398717 0.4013002054515486 >10 0.27663496035878143 3.8694439043978477 >50 0.012810157111133966 0.6890477411007454 >100 0.0053931720882852 0.7163520365539191 >500 1.550467691382162E-4 0.08107426161633333 >1k 1.5504676912716093E-4 0.4217856255050218 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4598 0.18937880621333825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3539 0.14576154745302394 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.11872131825442E-5 2 0.0 4.11872131825442E-5 0.0 0.0 4.11872131825442E-5 3 0.0 4.11872131825442E-5 0.0 0.0 4.11872131825442E-5 4 0.0 4.11872131825442E-5 0.0 0.0 4.11872131825442E-5 5 0.0 8.23744263650884E-5 0.0 0.0 4.11872131825442E-5 6 0.0 8.23744263650884E-5 0.0 0.0 4.11872131825442E-5 7 0.0 8.23744263650884E-5 0.0 4.11872131825442E-5 4.11872131825442E-5 8 0.0 1.235616395476326E-4 0.0 4.11872131825442E-5 4.11872131825442E-5 9 0.0 1.235616395476326E-4 0.0 4.11872131825442E-5 4.11872131825442E-5 10-11 4.11872131825442E-5 1.235616395476326E-4 0.0 4.11872131825442E-5 4.11872131825442E-5 12-13 4.11872131825442E-5 1.235616395476326E-4 0.0 4.11872131825442E-5 4.11872131825442E-5 14-15 4.11872131825442E-5 1.235616395476326E-4 0.0 4.11872131825442E-5 6.17808197738163E-5 16-17 4.11872131825442E-5 1.235616395476326E-4 0.0 8.23744263650884E-5 8.23744263650884E-5 18-19 4.11872131825442E-5 1.441552461389047E-4 0.0 1.235616395476326E-4 8.23744263650884E-5 20-21 4.11872131825442E-5 1.647488527301768E-4 0.0 1.647488527301768E-4 8.23744263650884E-5 22-23 4.11872131825442E-5 2.05936065912721E-4 0.0 2.2652967250399308E-4 8.23744263650884E-5 24-25 4.11872131825442E-5 2.05936065912721E-4 0.0 4.942465581905304E-4 8.23744263650884E-5 26-27 4.11872131825442E-5 2.05936065912721E-4 0.0 9.267122966072445E-4 8.23744263650884E-5 28-29 4.11872131825442E-5 2.05936065912721E-4 0.0 0.0023476711514050196 1.0296803295636051E-4 30-31 4.11872131825442E-5 2.05936065912721E-4 0.0 0.006116301157607814 1.235616395476326E-4 32-33 4.11872131825442E-5 2.05936065912721E-4 0.0 0.018678401178283795 1.235616395476326E-4 34-35 4.11872131825442E-5 2.2652967250399308E-4 0.0 0.03842766989931374 1.235616395476326E-4 36-37 4.11872131825442E-5 2.471232790952652E-4 0.0 0.06859730355552737 1.235616395476326E-4 38-39 4.11872131825442E-5 2.471232790952652E-4 0.0 0.11174090936424241 1.235616395476326E-4 40-41 4.11872131825442E-5 2.471232790952652E-4 0.0 0.15622309960139014 1.235616395476326E-4 42-43 4.11872131825442E-5 2.6771688568653733E-4 0.0 0.20457688787769707 1.235616395476326E-4 44-45 4.11872131825442E-5 3.0890409886908147E-4 0.0 0.2698792143786209 1.235616395476326E-4 46-47 4.11872131825442E-5 3.294977054603536E-4 0.0 0.3490204445088796 1.647488527301768E-4 48-49 4.11872131825442E-5 3.294977054603536E-4 0.0 0.4267407157843405 1.647488527301768E-4 50-51 4.11872131825442E-5 4.11872131825442E-4 0.0 0.5035342747631941 1.647488527301768E-4 52-53 4.11872131825442E-5 4.11872131825442E-4 0.0 0.5845701166998498 1.647488527301768E-4 54-55 4.11872131825442E-5 4.11872131825442E-4 0.0 0.6754908898003162 1.647488527301768E-4 56-57 4.11872131825442E-5 4.11872131825442E-4 0.0 0.7856460914570307 1.647488527301768E-4 58-59 4.11872131825442E-5 4.11872131825442E-4 0.0 0.9044712014886707 1.647488527301768E-4 60-61 4.11872131825442E-5 4.11872131825442E-4 0.0 1.0291243021856407 1.647488527301768E-4 62-63 4.11872131825442E-5 4.11872131825442E-4 0.0 1.1510384532059714 1.647488527301768E-4 64-65 4.11872131825442E-5 4.942465581905304E-4 0.0 1.2853911426074305 1.647488527301768E-4 66-67 4.11872131825442E-5 4.942465581905304E-4 0.0 1.4383398587608085 1.647488527301768E-4 68-69 4.11872131825442E-5 4.942465581905304E-4 0.0 1.6080517706794821 1.647488527301768E-4 70-71 4.11872131825442E-5 4.942465581905304E-4 0.0 1.7932294811482006 1.647488527301768E-4 72-73 4.11872131825442E-5 4.942465581905304E-4 0.0 1.9818051367044793 1.647488527301768E-4 74-75 4.11872131825442E-5 4.942465581905304E-4 0.0 2.1737375501351353 1.647488527301768E-4 76-77 4.11872131825442E-5 4.942465581905304E-4 0.0 2.379076401456709 1.647488527301768E-4 78-79 4.11872131825442E-5 4.942465581905304E-4 0.0 2.6108368500348855 1.647488527301768E-4 80-81 4.11872131825442E-5 4.942465581905304E-4 0.0 2.8608020468397464 1.647488527301768E-4 82-83 4.11872131825442E-5 4.942465581905304E-4 0.0 3.112888385123508 1.647488527301768E-4 84-85 4.11872131825442E-5 4.942465581905304E-4 0.0 3.3735828509624217 1.647488527301768E-4 86-87 4.11872131825442E-5 5.354337713730746E-4 0.0 3.651534759124821 1.647488527301768E-4 88-89 4.11872131825442E-5 5.354337713730746E-4 0.0 3.95489917782085 1.647488527301768E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2790 0.0 52.85272 1 GTATCAA 4970 0.0 40.45887 1 TCAACGC 5720 0.0 33.990185 4 ATCAACG 5680 0.0 33.885406 3 CAACGCA 5790 0.0 33.541992 5 AACGCAG 6100 0.0 32.128933 6 TATCAAC 6185 0.0 31.892412 2 ACGCAGA 6920 0.0 27.77985 7 CGCAGAG 6895 0.0 27.397495 8 GCAGAGT 7795 0.0 22.830223 9 GTGGTAT 1335 0.0 20.800203 1 GAGTACT 5280 0.0 20.687864 12-13 CAGAGTA 7825 0.0 20.279495 10-11 TGGTATC 1310 0.0 20.101168 2 GTACATG 5220 0.0 19.810896 1 AGAGTAC 7080 0.0 19.55714 10-11 TACATGG 5045 0.0 19.454613 2 GTACTTT 6030 0.0 18.264675 14-15 ACATGGG 5105 0.0 17.96228 3 TAGACCG 280 6.984792E-7 17.000015 5 >>END_MODULE