FastQCFastQC Report
Sat 18 Jun 2016
SRR1931129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2427938
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57590.23719716071827204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47070.19386821245023556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29860.12298501856307699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.056.9581871
GTATCAA55050.043.5320741
ATCAACG66550.035.322173
TCAACGC66700.035.2427334
CAACGCA67650.034.8173075
TATCAAC70700.033.517482
AACGCAG71700.032.850646
ACGCAGA82600.027.8832237
CGCAGAG84400.027.1760258
GCAGAGT93650.023.731189
GAGTACT64950.020.72789412-13
GTACATG57950.020.6767961
AGAGTAC86900.020.46507510-11
CAGAGTA92050.020.06813810-11
TACATGG57650.019.9345442
ACATGGG57100.019.0453783
GTGGTAT13000.019.019371
GTACTTT71400.018.15706814-15
AGTACTT70150.018.04059212-13
ACCGTCC2654.02144E-717.9198918
Produced by FastQC (version 0.11.5)