FastQCFastQC Report
Sat 18 Jun 2016
SRR1931128_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931128_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796412
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66930.2393424144940016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51010.18241231978692696No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30220.10806705163616806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34850.055.0206341
GTATCAA68450.042.929461
ATCAACG80400.035.3753243
TCAACGC81950.034.941684
CAACGCA81700.034.880975
TATCAAC86850.033.5590742
AACGCAG86200.033.264096
ACGCAGA97850.028.878227
CGCAGAG97950.028.7020078
GCAGAGT107900.024.7330939
GTACATG74250.024.4041521
GTGGTAT17900.024.3700681
TGGTATC17300.023.2760582
TACATGG79400.022.157552
GAGTACT64400.021.49783712-13
CAGAGTA106400.021.10153210-11
AGAGTAC96500.021.09745610-11
ACATGGG77900.021.0119763
GTACTTT78250.018.1034514-15
AGTACTT73050.017.356612-13
Produced by FastQC (version 0.11.5)