##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931128_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2796412 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.929044790252654 31.0 28.0 31.0 24.0 34.0 2 28.614954091171114 31.0 28.0 33.0 20.0 34.0 3 28.685149040985376 31.0 28.0 34.0 19.0 34.0 4 31.849348379280308 35.0 32.0 37.0 19.0 37.0 5 31.972433961805343 35.0 32.0 37.0 19.0 37.0 6 32.259960620967156 35.0 32.0 37.0 23.0 37.0 7 31.918543118825124 35.0 32.0 37.0 19.0 37.0 8 32.091102097974115 35.0 32.0 37.0 22.0 37.0 9 33.142387101757535 37.0 32.0 39.0 19.0 39.0 10-11 33.10271662401678 37.0 32.0 39.0 20.0 39.0 12-13 33.19178021693513 37.0 32.0 39.0 22.0 39.0 14-15 33.940907312656364 38.0 32.0 40.0 19.0 41.0 16-17 33.9085363673164 38.0 32.0 40.0 19.0 41.0 18-19 33.960091717529465 38.0 32.0 40.0 20.0 41.0 20-21 34.19850347516747 38.0 32.0 40.0 23.0 41.0 22-23 34.098310442095084 38.0 32.0 40.0 23.0 41.0 24-25 33.98130246902102 38.0 32.0 40.0 21.0 41.0 26-27 33.625463987423885 38.0 32.0 40.0 18.5 41.0 28-29 33.59759756430741 38.0 32.0 40.0 19.0 41.0 30-31 33.331202269193525 37.5 31.5 40.0 17.5 41.0 32-33 33.123368981394734 37.0 31.0 40.0 17.5 41.0 34-35 33.102836420384406 37.0 31.0 40.0 18.0 41.0 36-37 32.735368929900176 37.0 30.5 40.0 16.0 41.0 38-39 32.56280118952429 37.0 30.0 40.0 15.5 41.0 40-41 32.43880586980745 37.0 30.0 40.0 15.0 41.0 42-43 32.651761078124395 37.0 30.0 40.0 15.0 41.0 44-45 32.70135838352861 37.0 30.5 40.0 14.0 41.0 46-47 32.45894703641667 37.0 30.0 40.0 10.5 41.0 48-49 32.224377702570294 37.0 30.0 40.0 9.0 41.0 50-51 31.721099215709273 36.5 30.0 39.5 8.0 40.5 52-53 31.666468853659616 36.0 30.0 39.5 7.5 40.5 54-55 31.668103090674766 36.0 30.0 40.0 6.5 41.0 56-57 31.42832118443205 36.0 29.5 40.0 2.0 41.0 58-59 31.270067679583697 36.0 29.0 40.0 2.0 41.0 60-61 31.11196275799131 35.5 29.0 39.5 2.0 41.0 62-63 30.950243562107442 35.0 29.0 39.0 2.0 41.0 64-65 30.60770909293766 35.0 29.0 39.0 2.0 40.5 66-67 30.388362301406232 35.0 29.0 39.0 2.0 40.0 68-69 30.203050194320436 35.0 29.0 39.0 2.0 40.0 70-71 29.89113388871168 34.0 28.5 38.0 2.0 40.0 72-73 29.565329250482407 34.0 28.0 37.5 2.0 40.0 74-75 29.161707037446554 34.0 28.0 37.0 2.0 39.5 76-77 28.838165656562765 34.0 28.0 36.5 2.0 39.0 78-79 28.421167553278988 34.0 27.5 36.0 2.0 39.0 80-81 28.02197959385098 33.0 26.5 35.5 2.0 37.5 82-83 27.686373645943448 33.0 26.0 35.0 2.0 37.0 84-85 27.347411969337852 33.0 26.0 35.0 2.0 37.0 86-87 27.011631690895335 33.0 26.0 35.0 2.0 36.0 88-89 26.660845218801807 33.0 25.0 35.0 2.0 36.0 90-91 26.275756934242878 32.5 25.0 35.0 2.0 36.0 92-93 25.91956085154834 32.0 24.0 35.0 2.0 35.0 94-95 25.62688616698827 32.0 23.0 35.0 2.0 35.0 96-97 25.24658598232306 32.0 20.0 35.0 2.0 35.0 98-99 24.882208522921516 32.0 18.5 35.0 2.0 35.0 100-101 24.283647044856053 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.987990435626706 1101 2 5.603578924492833 1101 3 5.21378414463312 1101 4 6.5345933784233345 1101 5 6.003875145919295 1101 6 5.575920429757602 1101 7 6.085997682432225 1101 8 5.520523148197185 1101 9 6.208147850812679 1101 10-11 6.099058048415749 1101 12-13 6.03011143371214 1101 14-15 6.291292799741239 1101 16-17 6.235004513539479 1101 18-19 6.166636169223718 1101 20-21 5.624757618986131 1101 22-23 5.679444031084188 1101 24-25 5.62363258029194 1101 26-27 5.8604573009660434 1101 28-29 5.793003381160336 1101 30-31 5.834514195527731 1101 32-33 5.668206462707275 1101 34-35 5.53405792194825 1101 36-37 5.646916038081443 1101 38-39 5.5834294541771 1101 40-41 5.597610623554555 1101 42-43 5.559070526418022 1101 44-45 5.518731876704901 1101 46-47 5.194467611531188 1101 48-49 5.192255629030278 1101 50-51 5.170802895184259 1101 52-53 5.0266728720093035 1101 54-55 5.196603309828447 1101 56-57 5.113413171944121 1101 58-59 5.096312295941537 1101 60-61 4.9208292540330305 1101 62-63 4.9433900574322465 1101 64-65 4.928959046639722 1101 66-67 4.8596747702595735 1101 68-69 4.839233642608452 1101 70-71 4.670235655286536 1101 72-73 4.509495377608491 1101 74-75 4.6775485679941795 1101 76-77 4.546646689190737 1101 78-79 4.511321453930705 1101 80-81 4.481775431981706 1101 82-83 4.346485821102831 1101 84-85 4.289619723692287 1101 86-87 4.103375285250799 1101 88-89 4.164669048158977 1101 90-91 4.097406563357588 1101 92-93 4.052421767018707 1101 94-95 3.9944036502799527 1101 96-97 3.896462778372687 1101 98-99 3.705834782424116 1101 100-101 3.7038981200476258 1102 1 5.184002917052396 1102 2 5.717260384337919 1102 3 5.480058090517208 1102 4 6.808753838785822 1102 5 6.418490434709973 1102 6 5.960512881387935 1102 7 6.510302993728718 1102 8 6.039582762488763 1102 9 6.786937885935011 1102 10-11 6.613930199505482 1102 12-13 6.691930062437525 1102 14-15 6.900215150528677 1102 16-17 6.806257855640652 1102 18-19 6.87474158495689 1102 20-21 6.1852865678268145 1102 22-23 6.3572164139226714 1102 24-25 6.048286518300259 1102 26-27 6.275442841360547 1102 28-29 6.237465839710641 1102 30-31 6.449693706822693 1102 32-33 6.203384546679715 1102 34-35 6.20329273370734 1102 36-37 6.3644413045574595 1102 38-39 6.143994325029901 1102 40-41 6.289620349419419 1102 42-43 6.292049267277424 1102 44-45 6.25668645344005 1102 46-47 6.147478314219903 1102 48-49 5.940506123495581 1102 50-51 5.853253383341311 1102 52-53 5.57454827216937 1102 54-55 5.945604940735592 1102 56-57 5.927893900541289 1102 58-59 5.8840045623385535 1102 60-61 5.677445196588394 1102 62-63 5.556546347018141 1102 64-65 5.64782226868013 1102 66-67 5.640924610960184 1102 68-69 5.558202719092549 1102 70-71 5.559089285910762 1102 72-73 5.388318124860259 1102 74-75 5.482606220035887 1102 76-77 5.330679291085001 1102 78-79 5.292848833991009 1102 80-81 5.361394477051963 1102 82-83 5.143839157341787 1102 84-85 5.091882985684986 1102 86-87 5.067727068957911 1102 88-89 5.099453989205461 1102 90-91 5.0370360377588135 1102 92-93 5.071034148276944 1102 94-95 5.051085439237578 1102 96-97 4.986573862615302 1102 98-99 4.710233436790322 1102 100-101 4.57095214707245 1103 1 5.0045732756566395 1103 2 5.561587941812135 1103 3 5.279768040325976 1103 4 6.69782891824909 1103 5 6.240266062974442 1103 6 5.710740081783349 1103 7 6.189087014817705 1103 8 5.67994602927962 1103 9 6.315113728384496 1103 10-11 6.305031295838695 1103 12-13 6.364553151170323 1103 14-15 6.598285641441986 1103 16-17 6.421829278704195 1103 18-19 6.339158530669945 1103 20-21 5.663048528386021 1103 22-23 5.9565893767523335 1103 24-25 5.542245143042798 1103 26-27 5.825997473746117 1103 28-29 5.731303470514003 1103 30-31 5.999253866015039 1103 32-33 5.734733651252718 1103 34-35 5.701972816153248 1103 36-37 5.740220401354762 1103 38-39 5.4951108937399695 1103 40-41 5.529355860619713 1103 42-43 5.5283634817723275 1103 44-45 5.463093971564003 1103 46-47 5.527264818887595 1103 48-49 5.302172274097053 1103 50-51 5.194266988065067 1103 52-53 4.934658983120418 1103 54-55 5.227903557369558 1103 56-57 5.223438809109144 1103 58-59 5.279108145363615 1103 60-61 5.036906156316391 1103 62-63 5.048598104884668 1103 64-65 5.019014611565819 1103 66-67 4.959270082003179 1103 68-69 4.9863011278157 1103 70-71 4.848099324396532 1103 72-73 4.744069461900157 1103 74-75 4.783948540327263 1103 76-77 4.865099821714967 1103 78-79 4.777819423064457 1103 80-81 4.760390999658615 1103 82-83 4.61476822316321 1103 84-85 4.675695385570133 1103 86-87 4.550544432887328 1103 88-89 4.553090740135637 1103 90-91 4.536242123544412 1103 92-93 4.419903019583696 1103 94-95 4.379185919405707 1103 96-97 4.351710880917533 1103 98-99 4.258795770161733 1103 100-101 4.241633824890194 1104 1 4.500467201099013 1104 2 5.3228081266322675 1104 3 4.9722054616166815 1104 4 6.367664810284285 1104 5 5.993965252072144 1104 6 5.716917913776463 1104 7 6.227125091115578 1104 8 5.6386631658893265 1104 9 6.373184595906402 1104 10-11 6.234499174233356 1104 12-13 6.232288483371672 1104 14-15 6.265347104093404 1104 16-17 6.0913441335741965 1104 18-19 6.3290721623374715 1104 20-21 5.740486494754812 1104 22-23 5.928998209589324 1104 24-25 5.498958311419997 1104 26-27 5.820395950139709 1104 28-29 5.75156434916865 1104 30-31 5.921174506413067 1104 32-33 5.678039321222585 1104 34-35 5.666159684062496 1104 36-37 5.731645808371432 1104 38-39 5.483576572871797 1104 40-41 5.721526512165699 1104 42-43 5.745872391224733 1104 44-45 5.593772924903515 1104 46-47 5.603285702449348 1104 48-49 5.32465801682973 1104 50-51 5.191045137922231 1104 52-53 4.976188964598176 1104 54-55 5.335347240547662 1104 56-57 5.334270470048384 1104 58-59 5.164014859287214 1104 60-61 4.9150085890873925 1104 62-63 4.986660107726287 1104 64-65 5.058460856310404 1104 66-67 5.090393030583506 1104 68-69 5.042281327438879 1104 70-71 5.0144974785109575 1104 72-73 4.860631874622062 1104 74-75 4.86200031549291 1104 76-77 4.779237203906689 1104 78-79 4.80392209237651 1104 80-81 4.695655415811881 1104 82-83 4.4790057807294446 1104 84-85 4.475890756296746 1104 86-87 4.513500578200933 1104 88-89 4.6690256620199975 1104 90-91 4.637104564868391 1104 92-93 4.548007926340087 1104 94-95 4.337775049807654 1104 96-97 4.2973764925646485 1104 98-99 4.113340934057156 1104 100-101 4.140552230528204 1105 1 4.436323584071019 1105 2 5.054810208331379 1105 3 4.750283452069663 1105 4 6.134887775669721 1105 5 3.697801869007929 1105 6 4.9204538969814 1105 7 5.71231761670418 1105 8 1.0267320489086984 1105 9 0.7538607944307714 1105 10-11 2.2214260280255687 1105 12-13 5.420689200345226 1105 14-15 3.5527510560374758 1105 16-17 5.622946359779604 1105 18-19 6.0802481707552225 1105 20-21 3.2232660557032418 1105 22-23 5.283456430974482 1105 24-25 5.412011689065231 1105 26-27 5.602465232319833 1105 28-29 3.2900243086715157 1105 30-31 5.360376702807734 1105 32-33 5.55018756396791 1105 34-35 5.453419634975834 1105 36-37 5.654919469698697 1105 38-39 5.532313658568832 1105 40-41 5.631192391879363 1105 42-43 2.7690338201696996 1105 44-45 4.9931588362974075 1105 46-47 5.322321691338523 1105 48-49 5.218936944299955 1105 50-51 5.258824465184787 1105 52-53 5.074394509648389 1105 54-55 5.261403974427438 1105 56-57 5.284028952530154 1105 58-59 5.220437684208484 1105 60-61 5.177827207157506 1105 62-63 4.9675152338471875 1105 64-65 5.021082167705053 1105 66-67 5.09620454586593 1105 68-69 5.011516265120537 1105 70-71 4.846945647613641 1105 72-73 4.815707658322406 1105 74-75 4.941137092471948 1105 76-77 4.822143511025516 1105 78-79 4.754852793775214 1105 80-81 4.722965084975829 1105 82-83 4.511376012432155 1105 84-85 4.511638696387262 1105 86-87 4.439126572155416 1105 88-89 4.4668542148980634 1105 90-91 4.476789733141175 1105 92-93 4.455076734832609 1105 94-95 4.326165282495634 1105 96-97 4.259269588220494 1105 98-99 4.1868383022488445 1105 100-101 4.306890789583154 1106 1 4.244851330491844 1106 2 4.570762896453875 1106 3 4.573017496501823 1106 4 6.1031215858846615 1106 5 6.0116814148057145 1106 6 5.241910625670833 1106 7 5.869667109745496 1106 8 5.538624971965142 1106 9 6.14752841844431 1106 10-11 5.968364565844617 1106 12-13 6.273307057680061 1106 14-15 5.894469333683428 1106 16-17 5.88587118245286 1106 18-19 6.100168792877096 1106 20-21 5.337835550382799 1106 22-23 5.4491420730752225 1106 24-25 5.453906173569777 1106 26-27 5.703428850579048 1106 28-29 5.683050555078264 1106 30-31 5.70476240096702 1106 32-33 5.497759704150106 1106 34-35 5.073000802119896 1106 36-37 5.278430974499507 1106 38-39 5.240855291638773 1106 40-41 5.432684982680126 1106 42-43 2.2606098396545242 1106 44-45 4.445640738857929 1106 46-47 4.831100837303211 1106 48-49 4.601595559269882 1106 50-51 4.862857224877928 1106 52-53 4.6443150677765 1106 54-55 4.928559233563831 1106 56-57 4.900611691361323 1106 58-59 4.910977388506055 1106 60-61 4.664677126397358 1106 62-63 4.689573632006216 1106 64-65 4.651559874833701 1106 66-67 4.71187521342339 1106 68-69 4.759180332360078 1106 70-71 4.603982243093636 1106 72-73 4.429577221091343 1106 74-75 4.5582290617651715 1106 76-77 4.4800964653621165 1106 78-79 4.491073494225475 1106 80-81 4.47609406058908 1106 82-83 4.455934365795539 1106 84-85 4.47621542236336 1106 86-87 4.532829776707679 1106 88-89 4.6875281077417625 1106 90-91 4.697679924943316 1106 92-93 4.685740215889172 1106 94-95 4.588795893789769 1106 96-97 4.6307092945658574 1106 98-99 4.543843732760564 1106 100-101 4.5916048525991044 1107 1 3.035932779906627 1107 2 -0.19801845447033273 1107 3 1.1286520735649717 1107 4 2.6602180608382824 1107 5 2.7560909294533253 1107 6 4.132701756938154 1107 7 5.005191858365482 1107 8 4.47978429764828 1107 9 5.035648279183214 1107 10-11 2.5282777567101817 1107 12-13 5.0086640287513156 1107 14-15 3.577376924329112 1107 16-17 2.0704871837355228 1107 18-19 2.8301328323649493 1107 20-21 2.147087651896811 1107 22-23 4.22981272517444 1107 24-25 4.035628727911373 1107 26-27 3.376243161488599 1107 28-29 4.146585851351663 1107 30-31 3.510101506306121 1107 32-33 4.053106253544279 1107 34-35 4.6104756068566 1107 36-37 4.700712176542616 1107 38-39 4.757281458064931 1107 40-41 3.395931964974732 1107 42-43 3.9455015572843415 1107 44-45 4.355333184859898 1107 46-47 4.382579627472474 1107 48-49 2.2245470125784834 1107 50-51 3.83657398430897 1107 52-53 3.7858855343469635 1107 54-55 4.364508865942035 1107 56-57 3.164679523881535 1107 58-59 2.4211285028238336 1107 60-61 3.3787475357058625 1107 62-63 4.070497381977585 1107 64-65 4.3147126612007725 1107 66-67 4.315345288322035 1107 68-69 4.167961167926627 1107 70-71 4.001964617861724 1107 72-73 4.005275817028032 1107 74-75 4.262761918678983 1107 76-77 4.033711413024683 1107 78-79 3.947385193989554 1107 80-81 4.02056541815568 1107 82-83 3.826477667291222 1107 84-85 3.7431125684271755 1107 86-87 3.82992831309193 1107 88-89 3.807639081424167 1107 90-91 3.9043629685311245 1107 92-93 3.9123615883438774 1107 94-95 3.900039566342965 1107 96-97 3.973126665554659 1107 98-99 3.9531851629353234 1107 100-101 4.1562444458101275 1108 1 -2.702379894960284 1108 2 -1.7483969816166685 1108 3 -2.747183580991873 1108 4 -5.069768764421365 1108 5 -9.534067251992784 1108 6 -8.084872455973994 1108 7 -6.630596757238212 1108 8 -6.945847872846503 1108 9 -8.4322166856475 1108 10-11 -3.872073682448388 1108 12-13 -2.5053400944857778 1108 14-15 -7.515930341396437 1108 16-17 -6.509821173965726 1108 18-19 -5.3338881750601495 1108 20-21 -5.379209513194468 1108 22-23 -4.254916132806137 1108 24-25 -9.342451818967188 1108 26-27 -10.296838030878959 1108 28-29 -7.272955101683241 1108 30-31 -7.313584895411271 1108 32-33 -3.51065932063932 1108 34-35 -1.6953138374534866 1108 36-37 -5.455502078447523 1108 38-39 -6.320870289913614 1108 40-41 -4.107058650348218 1108 42-43 -5.327364678793256 1108 44-45 -2.359003943046705 1108 46-47 -2.997394767191331 1108 48-49 -1.7481868559751526 1108 50-51 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-0.31562738572265303 2313 54-55 -3.667585696343984 2313 56-57 -2.0174925014421845 2313 58-59 -1.964130568002247 2313 60-61 -0.47248900389591597 2313 62-63 -1.8612682014816642 2313 64-65 -1.6179097973421541 2313 66-67 -3.12609456784536 2313 68-69 -3.875089154357866 2313 70-71 -4.376779907127279 2313 72-73 -4.9185541085692 2313 74-75 -6.953807520718147 2313 76-77 -5.5075601283941324 2313 78-79 -6.834104009001859 2313 80-81 -9.116991722788796 2313 82-83 -7.077852612149513 2313 84-85 -6.832720876478437 2313 86-87 -5.17677176150211 2313 88-89 -7.08586549941473 2313 90-91 -7.327259164858315 2313 92-93 -5.876164318738731 2313 94-95 -6.49257944939829 2313 96-97 -7.066421562358812 2313 98-99 -7.901815506498332 2313 100-101 -7.2463376554464975 2314 1 -12.727329183355335 2314 2 -15.072433871742891 2314 3 -14.226246052761727 2314 4 -25.129021271258193 2314 5 -19.41796847317751 2314 6 -17.839682872589336 2314 7 -16.57521229346724 2314 8 -13.815878340871501 2314 9 -14.350749621500398 2314 10-11 -13.983146468999756 2314 12-13 -16.037253502880496 2314 14-15 -17.992915074534316 2314 16-17 -17.859862646614307 2314 18-19 -17.035133175758205 2314 20-21 -17.59981936643878 2314 22-23 -17.910717786784637 2314 24-25 -18.631101453104517 2314 26-27 -17.658027844210977 2314 28-29 -19.376952900758525 2314 30-31 -19.408922712718095 2314 32-33 -16.178303263579526 2314 34-35 -17.099084974132552 2314 36-37 -17.29252688812502 2314 38-39 -16.04618174539827 2314 40-41 -19.452033068704587 2314 42-43 -18.593902410691058 2314 44-45 -16.095254322870467 2314 46-47 -14.863006960971255 2314 48-49 -14.833008687834358 2314 50-51 -14.865585503564805 2314 52-53 -14.919695371233939 2314 54-55 -14.643530713526115 2314 56-57 -13.737934593018299 2314 58-59 -12.9102158285205 2314 60-61 -13.686197557810658 2314 62-63 -15.49254896678305 2314 64-65 -13.827624666850838 2314 66-67 -14.805856804308627 2314 68-69 -17.678516292836548 2314 70-71 -17.265467563856173 2314 72-73 -15.664630081449294 2314 74-75 -14.382664212461435 2314 76-77 -14.656069462470475 2314 78-79 -13.971421739103093 2314 80-81 -14.339012712851023 2314 82-83 -14.682477855950015 2314 84-85 -14.807922882608278 2314 86-87 -14.945881077003175 2314 88-89 -14.007756462542805 2314 90-91 -13.50126306566634 2314 92-93 -13.47572779974551 2314 94-95 -13.272508863348323 2314 96-97 -12.78913912528256 2314 98-99 -12.78455907480891 2314 100-101 -12.247591486597237 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 84314.0 3 14305.0 4 9545.0 5 7816.0 6 8531.0 7 9440.0 8 11337.0 9 13064.0 10 14424.0 11 15766.0 12 17617.0 13 18660.0 14 21121.0 15 23789.0 16 25961.0 17 27212.0 18 25454.0 19 23021.0 20 22885.0 21 24262.0 22 27779.0 23 34081.0 24 41444.0 25 51322.0 26 61066.0 27 70028.0 28 78295.0 29 86880.0 30 99128.0 31 111152.0 32 127665.0 33 153505.0 34 187005.0 35 227582.0 36 280708.0 37 340436.0 38 314966.0 39 84731.0 40 115.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.304161347751176 20.109149870342492 14.275635264552838 23.311053517353496 2 14.6509625013749 20.703610539384663 41.17170892250313 23.47371803673731 3 17.961587920521012 26.112032132604206 30.6619696954526 25.26441025142218 4 11.54581546060038 17.79911995298359 40.0120346465461 30.643029939869926 5 12.458568663325643 37.81375839286905 35.84967116507964 13.878001778725668 6 29.377425708835837 38.16998601531765 18.91586676216483 13.536721513681687 7 25.86358297574074 32.62973478305851 23.27810530995912 18.22857693124163 8 24.573843855572473 35.72419427071158 21.38089017652675 18.321071697189197 9 25.287942259048158 16.378002478662523 21.730037709750192 36.604017552539126 10-11 23.40123765905058 26.87305541167781 29.66674332186371 20.0589636074079 12-13 24.485994471544366 24.594334426050903 29.252952469832 21.66671863257273 14-15 21.754310834080197 26.144903893563033 27.16352721240309 24.937258059953688 16-17 21.11384776449552 29.120563963036922 27.79886063082426 21.966727641643295 18-19 20.943217501207563 28.20008134582021 29.805022558152324 21.051678594819908 20-21 21.89545262129162 27.38468670600312 29.877900675604923 20.841959997100332 22-23 21.558212033838267 27.359126990874593 29.583372188907358 21.49928878637978 24-25 21.403175764482864 27.505383379674303 29.601375768491767 21.490065087351063 26-27 21.261242578966673 27.63278348920369 29.654835992298175 21.451137939531474 28-29 21.248448773687695 27.615524631764426 29.708295169839705 21.427731424708178 30-31 21.473377326842026 27.488035583430804 29.628050812090763 21.41053627763641 32-33 21.34471450831341 27.639577909514507 29.47944941668853 21.536258165483556 34-35 21.60162984812486 27.711787496570757 29.471194722942297 21.21538793236209 36-37 21.508483613410924 27.441265707925073 29.42997236644168 21.620278312222325 38-39 21.438489974748887 27.679386038410914 29.499045384689204 21.383078602151 40-41 21.554469137252052 27.445989430391688 29.493112714924496 21.506428717431763 42-43 21.44114557351615 27.562937399015297 29.34241607228102 21.65350095518754 44-45 21.54936632564439 27.72001609933915 29.182611353613325 21.54800622140314 46-47 21.57405814953817 27.73987207967791 28.972991914200076 21.713077856583848 48-49 21.503759802197624 27.89489556403297 28.947568589655827 21.65377604411358 50-51 21.47566875752936 28.013820022605078 28.74485247643037 21.765658743435186 52-53 21.54841637769843 28.108132582731777 28.617967047598867 21.725483991970933 54-55 21.484678359804132 28.033739282277104 28.644581781476287 21.837000576442477 56-57 21.5505231793663 28.09744966876369 28.507795220079757 21.844231931790254 58-59 21.49017625210109 28.16601544594252 28.591606832641688 21.752201469314702 60-61 21.687857354440805 28.112903497332077 28.50398532023865 21.69525382798847 62-63 21.513568100837787 28.1049609285041 28.568912592278963 21.81255837837915 64-65 21.457442608599877 28.000022886470234 28.62012106942754 21.92241343550235 66-67 21.4582114509593 28.02271267610066 28.532294239904566 21.986781633035477 68-69 21.49411460113889 28.005154462218012 28.512572539382607 21.98815839726049 70-71 21.53833197683317 27.855730843666816 28.440229837377323 22.165707342122694 72-73 21.57545097074394 27.937549974753363 28.393759574769383 22.093239479733313 74-75 21.45985641600737 28.07184706688428 28.355639297785878 22.11265721932247 76-77 21.544429075543945 27.964083976180902 28.297725800060935 22.19376114821421 78-79 21.536865812333804 28.025895325867577 28.25549311045726 22.18174575134136 80-81 21.553905504625213 28.0626209585712 28.226044660085854 22.157428876717734 82-83 21.626087286136663 27.9658362215582 28.319610987222198 22.08846550508294 84-85 21.652961008606745 27.997555438898132 28.13210285179723 22.217380700697895 86-87 21.5211492441028 27.947580685535605 28.265291380526186 22.265978689835404 88-89 21.587126646574255 27.80572033019455 28.314264850815974 22.292888172415225 90-91 21.587251806958346 27.84770269903004 28.26822370952492 22.296821784486692 92-93 21.64312697842807 27.825084429619096 28.262663012460255 22.26912557949258 94-95 21.432017170574294 27.731035341001252 28.358374946181037 22.478572542243416 96-97 21.45883725287976 27.668329988571067 28.348379995508534 22.52445276304064 98-99 21.477039148737738 27.700907448544775 28.21887475808286 22.603178644634625 100-101 21.487552621001484 27.6289223476369 28.21440474436528 22.669120286996336 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 341.0 1 251.0 2 185.0 3 223.0 4 214.0 5 222.5 6 371.0 7 703.0 8 1205.0 9 1563.0 10 1568.0 11 1535.5 12 1680.0 13 1880.0 14 2121.0 15 2390.5 16 2633.5 17 2869.5 18 3212.0 19 3744.0 20 4346.0 21 5204.5 22 6519.5 23 8327.0 24 10749.0 25 14131.0 26 18770.5 27 24284.5 28 30290.0 29 36942.5 30 44160.0 31 51538.0 32 58956.0 33 66225.0 34 73353.0 35 79688.0 36 86474.0 37 92790.0 38 95835.5 39 97349.5 40 98375.0 41 98951.5 42 99357.0 43 100019.0 44 100776.0 45 101902.0 46 101382.0 47 98896.5 48 97811.0 49 96408.5 50 93097.0 51 89022.0 52 84462.0 53 80076.5 54 75818.5 55 70251.5 56 63926.0 57 56952.5 58 50162.0 59 43576.5 60 36254.5 61 29342.5 62 23212.5 63 17824.5 64 13364.5 65 9972.5 66 7351.0 67 5489.0 68 4109.0 69 3107.5 70 2441.0 71 1869.5 72 1406.0 73 1085.0 74 847.5 75 641.5 76 509.5 77 392.0 78 289.0 79 213.0 80 155.5 81 122.5 82 101.0 83 84.5 84 67.5 85 51.0 86 48.5 87 36.5 88 23.5 89 24.0 90 23.0 91 14.5 92 10.0 93 9.5 94 6.5 95 4.0 96 3.5 97 2.0 98 1.0 99 2.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.048490708808287195 2 0.5145879791675905 3 0.0 4 0.5759523274825026 5 0.20204462003452997 6 0.09444244982499002 7 0.1059572051614712 8 0.1076736904290212 9 0.10620752592965559 10-11 0.1677149146835302 12-13 0.21888763172236425 14-15 0.12955887759028356 16-17 0.12383726003178358 18-19 0.16547990782474115 20-21 0.10690484806959775 22-23 0.10540292346049152 24-25 0.0939775683983619 26-27 0.1554849571522365 28-29 0.10933653553196022 30-31 0.13057804071789136 32-33 0.099877986505565 34-35 0.15198046639765528 36-37 0.13563809624618978 38-39 0.13102504208964916 40-41 0.1048128816497712 42-43 0.10661876719167276 44-45 0.08970423528435724 46-47 0.0916710413200916 48-49 0.09707081789092593 50-51 0.08403625789046822 52-53 0.09168892137496192 54-55 0.3083594262934074 56-57 0.3263825216026823 58-59 0.3385230788596244 60-61 0.16238665833217708 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2796412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.25098532371668 #Duplication Level Percentage of deduplicated Percentage of total 1 86.3778298408689 65.00016805649722 2 9.05546850431135 13.628658550346238 3 2.1589083347903157 4.87379938249667 4 0.8318437707250692 2.5038825352982927 5 0.4323434422888621 1.6267135015242153 6 0.25707943711276693 1.1607288569521301 7 0.18069633213943126 0.9518303926511644 8 0.11913738240911863 0.7172164332139684 9 0.09059688733614411 0.6135754535375935 >10 0.466137752567482 6.235497895050874 >50 0.021803224447009956 1.1062420910368882 >100 0.007865708665449098 0.961642829975714 >500 9.646077945372792E-5 0.04395184446165793 >1k 9.646077931344678E-5 0.14804258740053916 >5k 9.646077931344678E-5 0.428049589556744 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6693 0.2393424144940016 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5101 0.18241231978692696 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3022 0.10806705163616806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.5760109740624773E-5 2 0.0 0.0 0.0 0.0 3.5760109740624773E-5 3 0.0 0.0 0.0 0.0 3.5760109740624773E-5 4 0.0 0.0 0.0 3.5760109740624773E-5 3.5760109740624773E-5 5 0.0 0.0 0.0 3.5760109740624773E-5 3.5760109740624773E-5 6 3.5760109740624773E-5 0.0 0.0 3.5760109740624773E-5 3.5760109740624773E-5 7 3.5760109740624773E-5 0.0 0.0 7.152021948124955E-5 7.152021948124955E-5 8 3.5760109740624773E-5 0.0 0.0 7.152021948124955E-5 7.152021948124955E-5 9 3.5760109740624773E-5 0.0 0.0 7.152021948124955E-5 7.152021948124955E-5 10-11 3.5760109740624773E-5 0.0 0.0 1.0728032922187432E-4 7.152021948124955E-5 12-13 3.5760109740624773E-5 0.0 0.0 1.430404389624991E-4 1.2516038409218671E-4 14-15 3.5760109740624773E-5 8.940027435156193E-5 0.0 1.430404389624991E-4 1.6092049383281147E-4 16-17 5.364016461093716E-5 1.0728032922187432E-4 0.0 1.9668060357343626E-4 1.7880054870312385E-4 18-19 7.152021948124955E-5 1.0728032922187432E-4 0.0 2.1456065844374864E-4 2.503207681843734E-4 20-21 7.152021948124955E-5 1.0728032922187432E-4 0.0 2.1456065844374864E-4 3.576010974062477E-4 22-23 7.152021948124955E-5 1.0728032922187432E-4 0.0 2.5032076818437343E-4 3.576010974062477E-4 24-25 8.940027435156193E-5 1.6092049383281147E-4 0.0 4.112412620171849E-4 4.6488142662812204E-4 26-27 1.0728032922187432E-4 1.7880054870312385E-4 0.0 8.403625789046821E-4 4.6488142662812204E-4 28-29 1.0728032922187432E-4 1.7880054870312385E-4 0.0 0.00269988828541717 5.364016461093717E-4 30-31 1.0728032922187432E-4 2.3244071331406102E-4 0.0 0.008332105569565571 5.900418107203087E-4 32-33 1.0728032922187432E-4 2.5032076818437343E-4 0.0 0.0235301522093311 6.079218655906211E-4 34-35 1.0728032922187432E-4 2.860808779249982E-4 0.0 0.04516501860240909 6.079218655906211E-4 36-37 1.0728032922187432E-4 3.3972104253593535E-4 0.0 0.08144364993427292 6.436819753312459E-4 38-39 1.0728032922187432E-4 3.576010974062477E-4 0.0 0.13256272680849604 6.794420850718707E-4 40-41 1.0728032922187432E-4 4.291213168874973E-4 0.0 0.18407516488986603 6.794420850718707E-4 42-43 1.0728032922187432E-4 4.291213168874973E-4 0.0 0.2390026934514657 6.794420850718707E-4 44-45 1.0728032922187432E-4 4.6488142662812204E-4 0.0 0.3069290219037824 6.794420850718707E-4 46-47 1.0728032922187432E-4 5.006415363687469E-4 0.0 0.3893560748559225 6.794420850718707E-4 48-49 1.0728032922187432E-4 5.006415363687469E-4 0.0 0.4753591387821251 6.794420850718707E-4 50-51 1.0728032922187432E-4 5.006415363687469E-4 0.0 0.5618628442446965 6.794420850718707E-4 52-53 1.0728032922187432E-4 5.006415363687469E-4 0.0 0.6477943879514176 6.794420850718707E-4 54-55 1.0728032922187432E-4 5.006415363687469E-4 0.0 0.7437387623855141 6.973221399421831E-4 56-57 1.0728032922187432E-4 5.006415363687469E-4 0.0 0.8569195097145914 7.509623045531201E-4 58-59 1.0728032922187432E-4 5.006415363687469E-4 0.0 0.9821692940811297 7.867224142937449E-4 60-61 1.0728032922187432E-4 5.006415363687469E-4 0.0 1.114124099024035 7.867224142937449E-4 62-63 1.0728032922187432E-4 5.006415363687469E-4 0.0 1.2501197963676312 7.867224142937449E-4 64-65 1.430404389624991E-4 6.079218655906211E-4 0.0 1.3897630249047708 7.867224142937449E-4 66-67 1.430404389624991E-4 6.079218655906211E-4 0.0 1.5473220684219635 7.867224142937449E-4 68-69 1.430404389624991E-4 6.079218655906211E-4 0.0 1.7256934958082 8.046024691640574E-4 70-71 1.7880054870312385E-4 6.079218655906211E-4 0.0 1.9192629698342019 8.224825240343697E-4 72-73 1.7880054870312385E-4 6.079218655906211E-4 0.0 2.1146562094569754 8.224825240343697E-4 74-75 1.7880054870312385E-4 6.079218655906211E-4 0.0 2.314287022084013 8.403625789046821E-4 76-77 1.7880054870312385E-4 6.258019204609334E-4 0.0 2.5350341795128903 8.582426337749946E-4 78-79 1.7880054870312385E-4 6.436819753312459E-4 0.0 2.780777653650463 8.582426337749946E-4 80-81 1.7880054870312385E-4 6.436819753312459E-4 0.0 3.046367988694084 8.582426337749946E-4 82-83 1.7880054870312385E-4 6.436819753312459E-4 0.0 3.318198462887443 8.582426337749946E-4 84-85 1.7880054870312385E-4 6.436819753312459E-4 0.0 3.5911017403730208 9.118827983859317E-4 86-87 1.7880054870312385E-4 6.436819753312459E-4 0.0 3.8821890336617066 9.297628532562441E-4 88-89 1.7880054870312385E-4 6.436819753312459E-4 0.0 4.2035114997360905 9.655229629968689E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3485 0.0 55.020634 1 GTATCAA 6845 0.0 42.92946 1 ATCAACG 8040 0.0 35.375324 3 TCAACGC 8195 0.0 34.94168 4 CAACGCA 8170 0.0 34.88097 5 TATCAAC 8685 0.0 33.559074 2 AACGCAG 8620 0.0 33.26409 6 ACGCAGA 9785 0.0 28.87822 7 CGCAGAG 9795 0.0 28.702007 8 GCAGAGT 10790 0.0 24.733093 9 GTACATG 7425 0.0 24.404152 1 GTGGTAT 1790 0.0 24.370068 1 TGGTATC 1730 0.0 23.276058 2 TACATGG 7940 0.0 22.15755 2 GAGTACT 6440 0.0 21.497837 12-13 CAGAGTA 10640 0.0 21.101532 10-11 AGAGTAC 9650 0.0 21.097456 10-11 ACATGGG 7790 0.0 21.011976 3 GTACTTT 7825 0.0 18.10345 14-15 AGTACTT 7305 0.0 17.3566 12-13 >>END_MODULE