FastQCFastQC Report
Sat 18 Jun 2016
SRR1931128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796412
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83570.2988472371024012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69340.24796060094149217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43640.15605711890808652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.055.9464871
GTATCAA78450.043.280971
ATCAACG96650.034.2477533
TCAACGC97150.034.070274
CAACGCA97750.033.811965
TATCAAC101650.033.1237872
AACGCAG101400.032.6416936
ACGCAGA117550.027.9147197
CGCAGAG118300.027.4968958
GTGGTAT18650.024.988431
GCAGAGT132500.023.833279
TGGTATC19200.023.0029142
GTACATG83200.022.977061
TACATGG82950.022.3279762
ACATGGG85150.021.3606913
AGAGTAC120650.021.15577710-11
GAGTACT81600.021.1539812-13
TATAGCG1150.00373034920.6466715
CAGAGTA130800.020.22205510-11
AGTACTT88550.019.54730212-13
Produced by FastQC (version 0.11.5)