##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931128_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2796412 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.298753188013784 31.0 31.0 34.0 30.0 34.0 2 31.308810361277235 31.0 31.0 34.0 28.0 34.0 3 31.461920847142697 31.0 31.0 34.0 28.0 34.0 4 35.08551565363044 35.0 35.0 37.0 32.0 37.0 5 34.98409461838956 35.0 35.0 37.0 32.0 37.0 6 35.09643822154961 36.0 35.0 37.0 32.0 37.0 7 34.96656537019581 36.0 35.0 37.0 32.0 37.0 8 35.00384707260589 36.0 35.0 37.0 32.0 37.0 9 36.40698902736793 38.0 35.0 39.0 32.0 39.0 10-11 36.293678113239395 38.0 35.0 39.0 32.0 39.0 12-13 36.213766605206956 38.0 35.0 39.0 32.0 39.0 14-15 37.16995921917085 39.0 36.0 40.5 31.5 41.0 16-17 37.20193161808775 39.0 36.0 40.5 31.5 41.0 18-19 37.2778290538018 39.0 36.0 40.5 31.5 41.0 20-21 37.27492336608483 39.0 36.0 40.0 31.0 41.0 22-23 37.159024671614915 39.0 36.0 40.0 31.0 41.0 24-25 37.018211372287055 39.0 36.0 40.0 31.0 41.0 26-27 36.77841838041033 39.0 36.0 40.0 30.5 41.0 28-29 36.50491486948275 38.0 35.0 40.0 30.0 41.0 30-31 36.284530677167744 38.0 35.0 40.0 30.0 41.0 32-33 36.131604177066905 38.0 35.0 40.0 30.0 41.0 34-35 35.91394258070699 38.0 34.5 40.0 29.0 41.0 36-37 36.168286540037734 38.0 35.0 40.0 30.0 41.0 38-39 36.16028342747778 38.0 35.0 40.0 30.0 41.0 40-41 36.00663886437334 38.0 35.0 40.0 29.5 41.0 42-43 35.82866616221072 38.0 35.0 40.0 29.0 41.0 44-45 35.599458162817214 38.0 34.0 40.0 28.0 41.0 46-47 35.396559054960434 38.0 34.0 40.0 27.0 41.0 48-49 35.12928656435461 38.0 34.0 40.0 26.5 41.0 50-51 34.77353122501262 38.0 33.0 40.0 25.0 41.0 52-53 34.66387570930178 38.0 33.0 40.0 25.0 41.0 54-55 34.43975619472381 38.0 33.0 40.0 24.0 41.0 56-57 34.23217233369046 38.0 33.0 40.0 24.0 41.0 58-59 33.994782778789386 37.0 33.0 40.0 23.5 41.0 60-61 33.71152551912951 37.0 32.0 40.0 23.0 41.0 62-63 33.45330587910508 36.5 32.0 40.0 22.5 41.0 64-65 33.220578727312 36.0 32.0 39.0 22.0 41.0 66-67 32.9851649184741 36.0 31.5 39.0 22.0 40.5 68-69 32.67151764475335 35.5 31.0 39.0 21.5 40.0 70-71 31.84583602130158 35.0 30.0 38.5 18.0 40.0 72-73 31.81501009150297 35.0 31.0 38.0 20.0 40.0 74-75 31.50341062046651 35.0 30.0 37.0 18.5 39.5 76-77 30.779836089960995 34.0 29.5 36.0 18.0 39.0 78-79 30.806394765864255 34.0 30.0 36.0 18.0 39.0 80-81 30.524639073212388 34.0 30.0 36.0 15.5 38.5 82-83 30.16513553796794 34.0 29.5 35.5 12.0 37.0 84-85 29.800903801013582 34.0 29.0 35.0 8.5 37.0 86-87 29.460084744308062 34.0 29.0 35.0 7.0 36.5 88-89 29.140476439094094 34.0 29.0 35.0 2.0 36.0 90-91 28.826419533316262 34.0 29.0 35.0 2.0 36.0 92-93 28.48959112605725 34.0 29.0 35.0 2.0 35.5 94-95 28.10548034409808 33.0 28.5 35.0 2.0 35.0 96-97 27.74281847596134 33.0 27.0 35.0 2.0 35.0 98-99 27.271087736714044 33.0 27.0 35.0 2.0 35.0 100-101 26.596671198664573 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.825671867092808 1101 2 0.7160190470752106 1101 3 0.5625634120846357 1101 4 0.8133196166275525 1101 5 0.8352519508617533 1101 6 0.891622121692528 1101 7 0.8110008844325662 1101 8 0.7562479786777914 1101 9 1.0595766264159039 1101 10-11 0.8778902267842312 1101 12-13 1.0322329143966584 1101 14-15 1.4433956659195104 1101 16-17 1.1540271935293305 1101 18-19 1.0138545628658875 1101 20-21 0.9714980093413459 1101 22-23 0.8939230186781657 1101 24-25 0.7797868233910492 1101 26-27 1.1712984949552592 1101 28-29 0.9455593135739591 1101 30-31 1.109619744558394 1101 32-33 1.173122832631158 1101 34-35 1.0098640296774093 1101 36-37 1.292642818277848 1101 38-39 1.0164905302408798 1101 40-41 1.0817332751196318 1101 42-43 1.1921400630401422 1101 44-45 1.0341727318762821 1101 46-47 1.1428260375580876 1101 48-49 0.9816218573234252 1101 50-51 0.960417875830295 1101 52-53 0.8769787411747032 1101 54-55 0.9338388040550711 1101 56-57 0.7918556617868049 1101 58-59 1.0474817207891007 1101 60-61 1.0005021325582035 1101 62-63 0.8727338987379696 1101 64-65 0.8160877435173788 1101 66-67 0.6993818791516304 1101 68-69 0.5955714170049262 1101 70-71 0.9029430149811262 1101 72-73 0.4614419317059344 1101 74-75 0.17057063736547207 1101 76-77 0.20338995455999154 1101 78-79 0.22869046641427815 1101 80-81 0.22207505782609616 1101 82-83 0.13842576329021838 1101 84-85 0.25007467739152744 1101 86-87 0.055201395005838094 1101 88-89 -0.011840577739771874 1101 90-91 0.09790827740125252 1101 92-93 -0.08376364092260147 1101 94-95 -0.11514038024185069 1101 96-97 -0.15897310902037276 1101 98-99 -0.1650733968672391 1101 100-101 -0.3308385521019872 1102 1 0.9858027187965455 1102 2 0.9058241888771548 1102 3 0.6582051719733037 1102 4 0.8257471194758281 1102 5 0.9315129074736959 1102 6 0.862822464887131 1102 7 0.9895083542994954 1102 8 0.9707833151053578 1102 9 1.2605562220758202 1102 10-11 1.1717937356400014 1102 12-13 1.2493785306049219 1102 14-15 1.628542654651305 1102 16-17 1.5283502906025532 1102 18-19 1.3100922458371684 1102 20-21 1.4244712766737564 1102 22-23 0.9847265773627925 1102 24-25 0.6844271428946413 1102 26-27 1.0630422147361642 1102 28-29 1.259257364450356 1102 30-31 1.45393306702114 1102 32-33 1.496883746232264 1102 34-35 1.5061458226493798 1102 36-37 1.5967277484282505 1102 38-39 1.3009313368182873 1102 40-41 1.443749897055035 1102 42-43 1.6201942464969648 1102 44-45 1.5706331759265169 1102 46-47 1.4905869970374184 1102 48-49 1.2716920255180924 1102 50-51 1.2233598360538736 1102 52-53 1.3355378813912964 1102 54-55 1.0975534141029044 1102 56-57 1.2727337216094625 1102 58-59 1.3728798338559898 1102 60-61 1.443899595046389 1102 62-63 1.3988136379584972 1102 64-65 1.3714601711508507 1102 66-67 1.1273509170852734 1102 68-69 1.1288923488808535 1102 70-71 1.5202749796141077 1102 72-73 1.0451847441175275 1102 74-75 0.8314046763950991 1102 76-77 0.9881850828023744 1102 78-79 0.9962106910646469 1102 80-81 0.9918200577368914 1102 82-83 0.9807434228381169 1102 84-85 0.8765538916887117 1102 86-87 0.8354373349232063 1102 88-89 0.7661299655730929 1102 90-91 0.9223891047640436 1102 92-93 0.7627201755339144 1102 94-95 0.7177184002130055 1102 96-97 0.8792380751348219 1102 98-99 0.7906596527220238 1102 100-101 0.7359405878979643 1103 1 0.6705726171807953 1103 2 0.4656477592388697 1103 3 0.3180928254188835 1103 4 0.5867029680031521 1103 5 0.5737874115302546 1103 6 0.6537763090220352 1103 7 0.6201354704960593 1103 8 0.5199064951972616 1103 9 0.7671021079346545 1103 10-11 0.7419492664340055 1103 12-13 0.8532078540757766 1103 14-15 1.2039299011118274 1103 16-17 0.985638352446081 1103 18-19 0.8592339252498036 1103 20-21 0.8794071708399258 1103 22-23 0.6511135390894367 1103 24-25 0.44050599574038785 1103 26-27 0.8218822558393768 1103 28-29 0.6103692035606301 1103 30-31 1.011376391448458 1103 32-33 1.0617986409753257 1103 34-35 0.9716793131251578 1103 36-37 1.1806876040641043 1103 38-39 0.9142255473810295 1103 40-41 1.0060527356051736 1103 42-43 1.2329958602603668 1103 44-45 1.0368889181994803 1103 46-47 1.1368584918711377 1103 48-49 1.0172559602481783 1103 50-51 0.8376219382615986 1103 52-53 0.5144303240655574 1103 54-55 0.6224499675776798 1103 56-57 0.6052808637457971 1103 58-59 0.8342418173088575 1103 60-61 0.8681163793064712 1103 62-63 0.6418443032467565 1103 64-65 0.6281860212754609 1103 66-67 0.5077906453404282 1103 68-69 0.4408998568511251 1103 70-71 0.8549806153094366 1103 72-73 0.36409896450346224 1103 74-75 0.19241640604207078 1103 76-77 0.33835417497544995 1103 78-79 0.3107174033845226 1103 80-81 0.2399174415138603 1103 82-83 0.26572893110486007 1103 84-85 0.3401295673210214 1103 86-87 0.3594396946941636 1103 88-89 0.21347179877555433 1103 90-91 0.2508859732900284 1103 92-93 -0.019216177702769954 1103 94-95 -0.05924025877422778 1103 96-97 -0.04116192208729075 1103 98-99 -0.16283850297352842 1103 100-101 -0.25887654901065815 1104 1 0.5198955284450086 1104 2 0.43649948369883873 1104 3 0.22587847547827522 1104 4 0.5790207125041178 1104 5 0.43366568664119853 1104 6 0.556442592190912 1104 7 0.5920745866570698 1104 8 0.4104119482416806 1104 9 0.8599406233559179 1104 10-11 0.5974534552418405 1104 12-13 0.6653398136921709 1104 14-15 1.0164865648926522 1104 16-17 0.8829888923778739 1104 18-19 0.6780697843118872 1104 20-21 0.7319590036384156 1104 22-23 0.43140450541289965 1104 24-25 0.4141755194495218 1104 26-27 0.25749575433637517 1104 28-29 0.5571304401719104 1104 30-31 0.8024527922440257 1104 32-33 0.8933830569426959 1104 34-35 0.758911861782444 1104 36-37 0.8705990161560706 1104 38-39 0.3131100383852825 1104 40-41 0.696261666976902 1104 42-43 0.8297809733347208 1104 44-45 0.5637648531806647 1104 46-47 0.6083961813658973 1104 48-49 0.37144126443840975 1104 50-51 0.36316538671358245 1104 52-53 0.16147389950209856 1104 54-55 0.5178343001066423 1104 56-57 0.5116154562675774 1104 58-59 0.5207563172470984 1104 60-61 0.6710686912448125 1104 62-63 -0.2888671784934118 1104 64-65 -1.095418948442358 1104 66-67 -0.2730406313798639 1104 68-69 0.17444662200605165 1104 70-71 0.786068543474677 1104 72-73 0.23711588492090385 1104 74-75 0.05418322344355886 1104 76-77 -0.076688124966374 1104 78-79 -0.23644406997614098 1104 80-81 -0.15536520721062175 1104 82-83 -0.25157250011672616 1104 84-85 -0.3578598778359705 1104 86-87 -0.2508002330101746 1104 88-89 -0.16028052631999046 1104 90-91 0.059574065110904684 1104 92-93 0.16577422704003553 1104 94-95 0.1769268275084066 1104 96-97 0.3214800050459701 1104 98-99 0.3298353752303491 1104 100-101 0.2356506747846936 1105 1 -0.2648229305923806 1105 2 -1.5894308071706114 1105 3 -0.30869566673663584 1105 4 0.3232584607322124 1105 5 0.5965365366641251 1105 6 0.04608967598056779 1105 7 0.03725314161935245 1105 8 -0.07886818555296315 1105 9 0.5013117405386467 1105 10-11 0.8531373906636972 1105 12-13 0.971586426825823 1105 14-15 1.2162598895859205 1105 16-17 0.8524240420189386 1105 18-19 0.6049879980422546 1105 20-21 0.9962435269381587 1105 22-23 0.28846737702082237 1105 24-25 -0.19538937006758061 1105 26-27 0.9195610196456414 1105 28-29 0.4617193361766425 1105 30-31 0.8801568221255067 1105 32-33 0.9591725804887616 1105 34-35 0.5438002167727731 1105 36-37 0.608965880255731 1105 38-39 0.5559223027256976 1105 40-41 0.7897417045182209 1105 42-43 1.1149860674343373 1105 44-45 0.9501237239599476 1105 46-47 0.9533304398426949 1105 48-49 0.8921101492262125 1105 50-51 0.6746794059718226 1105 52-53 0.7273186866016275 1105 54-55 -0.1778094160799597 1105 56-57 0.012673674368507193 1105 58-59 -0.6057653252236079 1105 60-61 0.0011111589172116965 1105 62-63 0.5865463507823776 1105 64-65 0.844275567265754 1105 66-67 0.6779036490634098 1105 68-69 0.6560347007417704 1105 70-71 0.264191123720515 1105 72-73 -0.3589271183342788 1105 74-75 -0.32686908303610807 1105 76-77 -0.11713788818573079 1105 78-79 -0.5854138310228514 1105 80-81 -0.3232795670186732 1105 82-83 -0.4114490129170285 1105 84-85 -0.2839734542278869 1105 86-87 -0.5729933813400763 1105 88-89 -0.802040698919118 1105 90-91 -0.8794920250463747 1105 92-93 -0.4479272184018903 1105 94-95 -0.698203120790172 1105 96-97 -0.8149334977033149 1105 98-99 -1.4478144016312129 1105 100-101 -1.0310515500312292 1106 1 0.3509821238919173 1106 2 0.40428468418638985 1106 3 -0.2364415756146201 1106 4 0.3309306743792675 1106 5 -7.711172473037209E-4 1106 6 0.3749940001679093 1106 7 0.45280663657536735 1106 8 0.5180411071110527 1106 9 0.8250431279492858 1106 10-11 0.12648023987132717 1106 12-13 0.33928393721200933 1106 14-15 0.03649657696097819 1106 16-17 -0.12302970584047301 1106 18-19 0.42629706109660503 1106 20-21 0.5281785003039516 1106 22-23 0.46221479487201833 1106 24-25 0.36789775336105635 1106 26-27 0.8332790131592347 1106 28-29 0.7331311732331329 1106 30-31 0.31368344865527575 1106 32-33 0.21794068752731022 1106 34-35 -1.150789173571333 1106 36-37 -0.810070155918666 1106 38-39 -0.6774794719496597 1106 40-41 0.4451865828404564 1106 42-43 0.6555306032618944 1106 44-45 0.3687431606611469 1106 46-47 -0.06811611383655958 1106 48-49 -0.07131276899230699 1106 50-51 0.5538901963461385 1106 52-53 -0.010971092568659913 1106 54-55 0.6601553067118004 1106 56-57 0.34561472586302244 1106 58-59 0.7940714586540594 1106 60-61 0.8804204997045417 1106 62-63 0.746954746253671 1106 64-65 0.7019553321782226 1106 66-67 0.4621070417573634 1106 68-69 0.5839002779784224 1106 70-71 0.907381674143771 1106 72-73 0.25925370503522416 1106 74-75 0.36003001541959634 1106 76-77 0.3959787558271408 1106 78-79 0.36813260797956104 1106 80-81 0.3583907600919076 1106 82-83 0.20325742844035588 1106 84-85 0.3065870160495656 1106 86-87 0.25709210794352444 1106 88-89 0.37890377880208703 1106 90-91 0.4809764285824585 1106 92-93 0.051142952051929313 1106 94-95 0.22682945204114446 1106 96-97 0.50486147264197 1106 98-99 0.521280604806563 1106 100-101 0.35337089335347827 1107 1 0.43961333395170143 1107 2 0.43856455775197745 1107 3 0.1804140555267253 1107 4 -1.2047996446843854 1107 5 0.08750621388717406 1107 6 0.4573584611038015 1107 7 0.6588815581264811 1107 8 0.4260308686722425 1107 9 0.879174578509307 1107 10-11 0.7117115209359355 1107 12-13 0.8427686139109696 1107 14-15 1.1060154619560478 1107 16-17 1.0275517893838853 1107 18-19 0.8922396502534724 1107 20-21 0.22718872729036832 1107 22-23 0.5388063911064691 1107 24-25 0.4928195907828936 1107 26-27 0.8997896409793853 1107 28-29 0.73422917135494 1107 30-31 0.9745594994122726 1107 32-33 1.09453371531081 1107 34-35 0.9248533056425785 1107 36-37 1.0159136045889028 1107 38-39 0.7841409843136589 1107 40-41 0.2764284230108629 1107 42-43 0.20513549572392975 1107 44-45 -0.039660032265857126 1107 46-47 0.2841062229095854 1107 48-49 0.6804082392763249 1107 50-51 -0.8143025016084593 1107 52-53 0.5562097865169875 1107 54-55 0.8225579613763614 1107 56-57 0.9858698851657977 1107 58-59 1.0471295337359479 1107 60-61 1.1903709108454308 1107 62-63 1.218239590157559 1107 64-65 1.114817514088024 1107 66-67 0.9676488544420323 1107 68-69 1.0964211785945608 1107 70-71 1.4798260727786428 1107 72-73 1.0615058180016277 1107 74-75 0.714115942719804 1107 76-77 0.7974415126470369 1107 78-79 0.8180212334634263 1107 80-81 0.9585321971381049 1107 82-83 1.073364107475605 1107 84-85 0.8372490788544873 1107 86-87 0.8997594273965568 1107 88-89 1.04519795640579 1107 90-91 1.1793882680657255 1107 92-93 1.0705123833261219 1107 94-95 1.014102408361719 1107 96-97 1.1980092199563828 1107 98-99 1.1904504781431022 1107 100-101 1.1138171930854917 1108 1 0.4984369429511126 1108 2 0.44826887869474064 1108 3 0.21441525856035426 1108 4 0.514219956194836 1108 5 0.5195488511127735 1108 6 0.5021192045492668 1108 7 0.6141881259346746 1108 8 0.6060728000880502 1108 9 0.7930408658877681 1108 10-11 0.5819167638850331 1108 12-13 0.819147550336794 1108 14-15 1.0489099811625877 1108 16-17 0.6668737948082466 1108 18-19 0.6991181387832199 1108 20-21 0.6568152625817021 1108 22-23 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1.1934344081376835 2311 80-81 1.2984944516323793 2311 82-83 1.4447393660429846 2311 84-85 1.4233312233110134 2311 86-87 1.2614594679143316 2311 88-89 1.5118592917863367 2311 90-91 1.8335466712389064 2311 92-93 2.0101984771994097 2311 94-95 1.9264432694710543 2311 96-97 2.0682585108953333 2311 98-99 2.271231191682439 2311 100-101 2.1707627926419306 2312 1 -0.1519442029278828 2312 2 -0.15267648070800988 2312 3 -0.45490293698670925 2312 4 0.13399089710691214 2312 5 -1.3781372841858612 2312 6 -0.3056791443086837 2312 7 0.13613834913313383 2312 8 -2.801172128737594 2312 9 -5.541531154510221 2312 10-11 -0.8494696834560642 2312 12-13 -5.9278808202472 2312 14-15 -2.2369545569865394 2312 16-17 -3.1212482584945036 2312 18-19 -2.569759244646498 2312 20-21 -3.4381534431316396 2312 22-23 -1.9333069526283495 2312 24-25 -3.3227424166020754 2312 26-27 -3.872125926038528 2312 28-29 -6.0969904610114085 2312 30-31 -6.569238091915054 2312 32-33 -1.8468680080328284 2312 34-35 0.48018652670294415 2312 36-37 0.7700399840501859 2312 38-39 0.5897879897570348 2312 40-41 -2.9203628898451726 2312 42-43 -4.95577500091693 2312 44-45 -3.732004083028084 2312 46-47 0.04176238091940121 2312 48-49 -1.528832985686691 2312 50-51 -6.86588951710074 2312 52-53 -1.5469248561418212 2312 54-55 0.5708948136784926 2312 56-57 0.9096135297968075 2312 58-59 1.2334730583926117 2312 60-61 1.3616600225482216 2312 62-63 1.2846032126030593 2312 64-65 1.2491592627905064 2312 66-67 1.219170315512315 2312 68-69 1.2690183167473705 2312 70-71 1.6970529676285153 2312 72-73 1.218465234107967 2312 74-75 1.1214597081602342 2312 76-77 1.1411022886944835 2312 78-79 0.9541914077324094 2312 80-81 1.0025648657876438 2312 82-83 -0.09352882169360655 2312 84-85 0.3839897992850574 2312 86-87 0.8058381734281497 2312 88-89 0.9616690964542514 2312 90-91 1.4415764496194612 2312 92-93 1.6123841221328092 2312 94-95 1.5202000830586861 2312 96-97 1.6600536271620854 2312 98-99 1.6197730685309608 2312 100-101 1.6640274265343962 2313 1 0.02971719180224497 2313 2 -0.07330354469049993 2313 3 -0.35167094342130767 2313 4 -0.8222584745143493 2313 5 -1.9454337474769403 2313 6 -1.9254887358818351 2313 7 -3.594428313150992 2313 8 -4.363109004709166 2313 9 -2.352839651737618 2313 10-11 -1.4011282166460646 2313 12-13 -4.703095242409439 2313 14-15 -3.0014382036714053 2313 16-17 -6.884467413798795 2313 18-19 -3.4018100408507053 2313 20-21 -3.629080040760428 2313 22-23 -2.3719810541434185 2313 24-25 -0.8945013357132936 2313 26-27 -3.344643539978101 2313 28-29 -4.861021675000472 2313 30-31 -4.883903098372855 2313 32-33 -7.898936437511331 2313 34-35 -3.6534551311341943 2313 36-37 -4.756603274702648 2313 38-39 -3.124116001704742 2313 40-41 -1.4229576106903394 2313 42-43 -2.6547023100638043 2313 44-45 -5.211165110398326 2313 46-47 -2.772558467333898 2313 48-49 -1.8220093725669173 2313 50-51 -0.448321207497564 2313 52-53 -0.5277486703427954 2313 54-55 -3.588891266862259 2313 56-57 -6.7446505516874815 2313 58-59 -2.1237940934468895 2313 60-61 -7.096940452312765 2313 62-63 -1.3858363044927096 2313 64-65 -1.6902108890257956 2313 66-67 -3.4788327935123675 2313 68-69 -5.6239716833299696 2313 70-71 -4.9571840452329425 2313 72-73 -0.39086493348547435 2313 74-75 0.8694972330053652 2313 76-77 -2.773964106770361 2313 78-79 -0.24272508943311522 2313 80-81 0.8808798053472771 2313 82-83 1.8548590484698124 2313 84-85 1.4678058563921184 2313 86-87 1.4968885133327134 2313 88-89 -2.4655627114977356 2313 90-91 0.781987583330725 2313 92-93 2.3914809581974943 2313 94-95 2.4574472814562256 2313 96-97 2.7365418213755817 2313 98-99 2.755974644698199 2313 100-101 2.9622115086373952 2314 1 -28.764097241187446 2314 2 -14.893030488128783 2314 3 -5.801935641108685 2314 4 -3.9006461405308777 2314 5 -1.184311800787988 2314 6 -0.8040064288325226 2314 7 -0.31617067406860855 2314 8 -4.9140516999817585 2314 9 -3.4806375527313946 2314 10-11 -1.2335792243548056 2314 12-13 -0.5805912951632308 2314 14-15 -1.806769307971578 2314 16-17 -1.9063398016505673 2314 18-19 -3.6072990349994924 2314 20-21 -2.7755925326337447 2314 22-23 -5.330372435215214 2314 24-25 -1.8304166324533284 2314 26-27 -4.071354787307904 2314 28-29 -2.65952636525774 2314 30-31 -3.4953547562162655 2314 32-33 -2.644594466674512 2314 34-35 -2.3594085071906647 2314 36-37 -7.110555164737015 2314 38-39 -9.960467817438005 2314 40-41 -7.061668486514613 2314 42-43 -4.124749677018386 2314 44-45 -4.781472531221212 2314 46-47 -6.056110060163839 2314 48-49 -5.119399546245745 2314 50-51 -2.677632993510386 2314 52-53 -3.5218510201152498 2314 54-55 -5.541477874088045 2314 56-57 -2.202623989042362 2314 58-59 -0.8903957466464831 2314 60-61 0.3306624082797853 2314 62-63 0.5827250403572961 2314 64-65 -0.24607603251980237 2314 66-67 0.7748126044629231 2314 68-69 1.131531242276047 2314 70-71 1.9572768073722386 2314 72-73 0.5992901700716899 2314 74-75 0.7914346343242151 2314 76-77 -0.35016917782079204 2314 78-79 -1.625375781362159 2314 80-81 -1.5336739236394443 2314 82-83 -1.6158384531555257 2314 84-85 -1.063121978659428 2314 86-87 0.1786475919990096 2314 88-89 0.48488773895271464 2314 90-91 0.9411777012837312 2314 92-93 0.7684197526620657 2314 94-95 1.0509273594723858 2314 96-97 1.181559003506166 2314 98-99 0.8495940372866642 2314 100-101 0.9662516312342149 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 15.0 4 60.0 5 126.0 6 260.0 7 740.0 8 1643.0 9 2990.0 10 4600.0 11 6950.0 12 9205.0 13 11658.0 14 14003.0 15 16872.0 16 19959.0 17 22324.0 18 20375.0 19 17058.0 20 16433.0 21 17517.0 22 19277.0 23 21800.0 24 26169.0 25 32289.0 26 40555.0 27 51324.0 28 64828.0 29 81314.0 30 100738.0 31 122732.0 32 148497.0 33 179669.0 34 219809.0 35 271113.0 36 339658.0 37 422495.0 38 381250.0 39 90019.0 40 78.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.165505083017486 21.40914549309602 15.248443802528152 23.17690562135834 2 14.776041584716415 21.9676142142145 40.359610815573674 22.896733385495413 3 18.009818660891433 26.72902984935851 31.134624655080913 24.126526834669143 4 12.564118151909598 18.31916034902017 39.09111715062223 30.025604348448002 5 12.121317234562675 38.661509567287126 36.19162324184917 13.025549956301022 6 29.389052829125323 39.26374225257223 18.202253459075415 13.14495145922704 7 25.768878119533174 33.399012734890285 23.123023359934088 17.709085785642458 8 24.544094360916773 36.75327526845115 20.737251878478567 17.965378492153516 9 25.101487191443894 16.406094667023314 21.46060737831192 37.03181076322087 10-11 23.473633355886044 27.427199568590037 29.37582158852129 19.723345487002632 12-13 24.413087198881996 24.972875956761737 29.378950598123595 21.235086246232672 14-15 21.588199449866472 26.615391437313242 27.127833809896394 24.66857530292389 16-17 20.91283401730503 29.64808118403154 27.80407536514648 21.63500943351695 18-19 20.911171172202092 28.48226584637743 29.912276874795275 20.694286106625203 20-21 21.729112162299405 27.64907316947574 29.937273191503973 20.684541476720884 22-23 21.457675049313192 27.604283632025613 29.714326787326044 21.22371453133515 24-25 21.31987039468531 27.76889365146414 29.779249629722095 21.13198632412846 26-27 21.30540354619174 27.818920592549517 29.709320425605952 21.166355435652786 28-29 21.23074029093229 27.81346244902105 29.812853061884265 21.142944198162397 30-31 21.53821946465929 27.629785255779854 29.6853040570744 21.146691222486456 32-33 21.331799684699675 27.814016077563092 29.556500069822377 21.297684167914856 34-35 21.56755252877657 27.74682696007541 29.60743420403294 21.078186307115075 36-37 21.492128201689688 27.65952349023289 29.540673192973046 21.307675115104377 38-39 21.395970735136824 27.73104691010663 29.67072558880357 21.202256765952974 40-41 21.596669653302854 27.599486544134123 29.61663714301398 21.187206659549044 42-43 21.497936791694098 27.672506493232486 29.365574621125294 21.463982093948125 44-45 21.55529585602029 27.808313134701336 29.228517154592666 21.407873854685715 46-47 21.607433955906092 27.791605838502164 29.028493873779578 21.57246633181217 48-49 21.557523605210083 27.961752388419132 28.92976361347937 21.550960392891415 50-51 21.63256548254127 28.040840533015697 28.72933644334319 21.59725754109984 52-53 21.690352335688104 28.051680359064022 28.624474164201132 21.633493141046742 54-55 21.65909662883645 27.99224280548291 28.723459628621274 21.625200937059365 56-57 21.675332925782836 28.07950202788854 28.57185258878304 21.67331245754558 58-59 21.58197280730002 28.091255984755982 28.696414340634867 21.63035686730913 60-61 21.714315038744942 28.072233295384386 28.60622454560946 21.607227120261214 62-63 21.650580239648693 28.053966998909097 28.573617687506157 21.721835073936056 64-65 21.559643362945796 28.001820379668967 28.622823770081396 21.815712487303834 66-67 21.557310449302708 28.03784290641303 28.519524762502847 21.88532188178142 68-69 21.599538254317377 28.06138514049324 28.44563679475115 21.893439810438235 70-71 21.653764004209197 27.90334938752958 28.437080633668394 22.005805974592825 72-73 21.692458112146298 27.958309551703763 28.34630773214089 22.002924604009046 74-75 21.570843198009143 28.10134947513458 28.255816843433674 22.071990483422606 76-77 21.540084222210464 28.09421501552704 28.147622739424662 22.218078022837837 78-79 21.577028273557023 28.136202415130963 28.04279699178751 22.243972319524506 80-81 21.58642341850881 28.200919502496692 28.03552849222224 22.17712858677226 82-83 21.614376780415377 28.113916545498892 28.142292334639063 22.129414339446672 84-85 21.652249677846054 28.126547183774637 27.930849948228293 22.29035319015102 86-87 21.438298334946737 28.174180999847305 28.02711518194858 22.360405483257384 88-89 21.552131233251327 28.034439037107383 27.969370992812475 22.44405873682882 90-91 21.560267532264117 28.065265256101384 27.945502978322278 22.428964233312225 92-93 21.58733723500259 28.065358652044853 27.93232688123285 22.41497723171971 94-95 21.4293428312932 28.026044292820384 27.912773254088307 22.631839621798104 96-97 21.398170074502985 27.95987910161572 27.957107679235932 22.684843144645363 98-99 21.335923123081884 28.04225197989104 27.846015149662094 22.775809747364974 100-101 21.305494513354088 28.010324227962673 27.82754927431799 22.85663198436525 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 378.0 1 286.5 2 211.0 3 252.0 4 223.0 5 208.0 6 380.0 7 775.5 8 1310.5 9 1728.5 10 1773.0 11 1691.0 12 1787.5 13 2021.0 14 2301.0 15 2588.0 16 2872.5 17 3163.0 18 3477.0 19 3834.0 20 4499.0 21 5473.5 22 6754.0 23 8508.0 24 10962.5 25 14701.0 26 19534.0 27 24989.5 28 31085.0 29 38186.0 30 45518.0 31 52530.0 32 59953.5 33 67070.5 34 73637.5 35 79403.0 36 86416.0 37 92880.5 38 95487.5 39 96982.0 40 97920.0 41 98437.0 42 98464.0 43 98981.5 44 100119.5 45 101072.5 46 100549.0 47 98855.5 48 97760.0 49 95897.5 50 92933.5 51 88829.0 52 84183.5 53 79919.5 54 75887.0 55 70337.0 56 63927.0 57 57227.0 58 50335.5 59 43667.5 60 36123.5 61 28790.5 62 22637.5 63 17415.5 64 12989.5 65 9498.5 66 6944.0 67 5078.5 68 3756.0 69 2921.5 70 2278.5 71 1710.0 72 1263.0 73 980.5 74 800.5 75 609.5 76 470.5 77 338.0 78 201.5 79 143.0 80 119.5 81 95.5 82 73.0 83 56.5 84 44.0 85 38.0 86 25.0 87 16.5 88 18.0 89 12.0 90 10.0 91 5.0 92 1.5 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12108373158175548 2 0.0 3 0.0037190514130249763 4 4.291213168874972E-4 5 2.860808779249982E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.01927469915019675 26-27 0.009208228258210879 28-29 0.005721617558499963 30-31 0.0022171268039187357 32-33 0.0010906833470890557 34-35 0.0014304043896249908 36-37 0.0013231240604031165 38-39 7.509623045531202E-4 40-41 0.0023780472977515473 42-43 0.0013946442798843661 44-45 6.436819753312459E-4 46-47 0.03465154633866541 48-49 0.018774057613828006 50-51 0.08664674590153382 52-53 0.0010906833470890557 54-55 0.038316957587079446 56-57 0.0010906833470890557 58-59 0.0013052440055328041 60-61 0.003987252236079662 62-63 0.003951492126339037 64-65 0.010441952044262433 66-67 0.006007698436424962 68-69 0.002413807407492172 70-71 0.005524936954926527 72-73 0.0048633749247249695 74-75 0.001019163127607806 76-77 0.0 78-79 1.7880054870312387E-5 80-81 3.039609327953106E-4 82-83 5.006415363687467E-4 84-85 1.7880054870312387E-5 86-87 0.0016092049383281146 88-89 0.003879971906857788 90-91 0.0013946442798843661 92-93 0.003129009602304668 94-95 7.867224142937449E-4 96-97 5.006415363687467E-4 98-99 0.0016449650480687395 100-101 0.0027535284500281075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2796412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.92857772886715 #Duplication Level Percentage of deduplicated Percentage of total 1 83.8715754195006 59.48891556384592 2 10.502776687271366 14.898940252641218 3 2.6741448099001577 5.690198640217501 4 1.05430886363793 2.991225127391061 5 0.52382106667704 1.8576941621910266 6 0.29343123388920395 1.248759604859268 7 0.2046055277952342 1.015866535738611 8 0.1487431449772455 0.8440111776123771 9 0.10710503725340989 0.6837127163983525 >10 0.574000698626884 7.390553745079205 >50 0.03309950925441375 1.5976764795739977 >100 0.011982543662360328 1.4286476687208884 >500 1.5204658288825817E-4 0.058990929861871516 >1k 1.520465827524841E-4 0.25512408828301575 >5k 1.0136438850165607E-4 0.5496833075856832 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8357 0.2988472371024012 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6934 0.24796060094149217 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4364 0.15605711890808652 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.5760109740624773E-5 0.0 0.0 0.0 3 0.0 3.5760109740624773E-5 0.0 0.0 0.0 4 0.0 7.152021948124955E-5 0.0 0.0 0.0 5 0.0 7.152021948124955E-5 0.0 0.0 0.0 6 0.0 7.152021948124955E-5 0.0 0.0 0.0 7 0.0 7.152021948124955E-5 0.0 3.5760109740624773E-5 3.5760109740624773E-5 8 0.0 7.152021948124955E-5 0.0 3.5760109740624773E-5 7.152021948124955E-5 9 0.0 2.1456065844374864E-4 0.0 7.152021948124955E-5 7.152021948124955E-5 10-11 0.0 2.1456065844374864E-4 0.0 7.152021948124955E-5 7.152021948124955E-5 12-13 0.0 2.1456065844374864E-4 0.0 7.152021948124955E-5 7.152021948124955E-5 14-15 0.0 2.5032076818437343E-4 0.0 7.152021948124955E-5 2.1456065844374864E-4 16-17 0.0 2.860808779249982E-4 0.0 1.0728032922187432E-4 2.1456065844374864E-4 18-19 0.0 3.0396093279531054E-4 0.0 1.0728032922187432E-4 2.5032076818437343E-4 20-21 0.0 3.2184098766562295E-4 0.0 1.0728032922187432E-4 3.2184098766562295E-4 22-23 0.0 3.576010974062477E-4 0.0 1.6092049383281147E-4 3.2184098766562295E-4 24-25 0.0 4.112412620171849E-4 0.0 3.3972104253593535E-4 3.576010974062477E-4 26-27 0.0 4.291213168874973E-4 0.0 8.046024691640574E-4 3.576010974062477E-4 28-29 0.0 4.291213168874973E-4 0.0 0.00277140850489842 4.112412620171849E-4 30-31 0.0 4.291213168874973E-4 0.0 0.008868507215674943 4.6488142662812204E-4 32-33 0.0 4.291213168874973E-4 0.0 0.025085716983048276 4.6488142662812204E-4 34-35 1.7880054870312387E-5 4.470013717578097E-4 0.0 0.04866950935699031 4.6488142662812204E-4 36-37 3.5760109740624773E-5 4.6488142662812204E-4 0.0 0.08766590902914162 4.6488142662812204E-4 38-39 3.5760109740624773E-5 4.6488142662812204E-4 0.0 0.14200339578002097 4.6488142662812204E-4 40-41 3.5760109740624773E-5 4.6488142662812204E-4 0.0 0.19669848362830655 5.006415363687467E-4 42-43 3.5760109740624773E-5 4.6488142662812204E-4 0.0 0.2542365002009718 5.364016461093716E-4 44-45 3.5760109740624773E-5 4.8276148149843445E-4 0.0 0.3256851994627401 5.364016461093716E-4 46-47 3.5760109740624773E-5 5.006415363687469E-4 0.0 0.41211738470583015 5.364016461093716E-4 48-49 3.5760109740624773E-5 5.006415363687469E-4 0.0 0.5026977426788327 5.364016461093716E-4 50-51 3.5760109740624773E-5 5.900418107203087E-4 0.0 0.5932602205969649 5.364016461093716E-4 52-53 3.5760109740624773E-5 6.079218655906211E-4 0.0 0.6818201323696222 5.364016461093716E-4 54-55 3.5760109740624773E-5 6.436819753312459E-4 0.0 0.7816087186008356 5.364016461093716E-4 56-57 3.5760109740624773E-5 6.794420850718707E-4 0.0 0.8979363555870881 6.079218655906211E-4 58-59 3.5760109740624773E-5 6.794420850718707E-4 0.0 1.025850268129303 6.794420850718707E-4 60-61 3.5760109740624773E-5 6.973221399421831E-4 0.0 1.1559813074754364 7.509623045531202E-4 62-63 3.5760109740624773E-5 7.152021948124954E-4 0.0 1.2885619143388027 7.509623045531202E-4 64-65 3.5760109740624773E-5 8.224825240343697E-4 0.0 1.4261846966755973 7.867224142937449E-4 66-67 3.5760109740624773E-5 8.224825240343697E-4 0.0 1.5817232939924446 7.867224142937449E-4 68-69 3.5760109740624773E-5 8.224825240343697E-4 0.0 1.7589861579767216 8.761226886453069E-4 70-71 3.5760109740624773E-5 8.582426337749945E-4 0.0 1.9501060644854906 9.655229629968689E-4 72-73 3.5760109740624773E-5 9.297628532562441E-4 0.0 2.1484137530521252 9.655229629968689E-4 74-75 3.5760109740624773E-5 9.834030178671814E-4 0.0 2.3512629755558194 0.0010370431824781184 76-77 3.5760109740624773E-5 0.0010906833470890555 0.0 2.57510338247726 0.0010728032922187431 78-79 3.5760109740624773E-5 0.0011085634019593678 0.0 2.8233679443515474 0.0010906833470890555 80-81 3.5760109740624773E-5 0.0011085634019593678 0.0 3.091586647461104 0.001162203566570305 82-83 3.5760109740624773E-5 0.0011085634019593678 0.0 3.367440134000283 0.0012158437311812422 84-85 3.5760109740624773E-5 0.0011085634019593678 0.0 3.64141621477808 0.0012158437311812422 86-87 3.5760109740624773E-5 0.0011085634019593678 0.0 3.9353464367911455 0.0012158437311812422 88-89 3.5760109740624773E-5 0.0011443235116999927 0.0 4.261210436802589 0.0012516038409218669 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3995 0.0 55.946487 1 GTATCAA 7845 0.0 43.28097 1 ATCAACG 9665 0.0 34.247753 3 TCAACGC 9715 0.0 34.07027 4 CAACGCA 9775 0.0 33.81196 5 TATCAAC 10165 0.0 33.123787 2 AACGCAG 10140 0.0 32.641693 6 ACGCAGA 11755 0.0 27.914719 7 CGCAGAG 11830 0.0 27.496895 8 GTGGTAT 1865 0.0 24.98843 1 GCAGAGT 13250 0.0 23.83327 9 TGGTATC 1920 0.0 23.002914 2 GTACATG 8320 0.0 22.97706 1 TACATGG 8295 0.0 22.327976 2 ACATGGG 8515 0.0 21.360691 3 AGAGTAC 12065 0.0 21.155777 10-11 GAGTACT 8160 0.0 21.15398 12-13 TATAGCG 115 0.003730349 20.646671 5 CAGAGTA 13080 0.0 20.222055 10-11 AGTACTT 8855 0.0 19.547302 12-13 >>END_MODULE