FastQCFastQC Report
Sat 18 Jun 2016
SRR1931127_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931127_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933029
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68060.2320468021284481No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50530.17227923760726538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36650.057.8290821
GTATCAA76500.045.255751
CAACGCA94500.035.8863225
ATCAACG94600.035.6613543
TCAACGC99600.034.3117184
AACGCAG105700.032.1551066
TATCAAC109800.031.3128262
CGCAGAG111950.029.4318168
ACGCAGA124050.026.7530947
GCAGAGT124300.025.1696729
GTACATG97950.022.5680731
GTGGTAT16050.022.4070261
CAGAGTA120800.021.41096310-11
TACATGG103500.021.2192782
AGAGTAC114800.020.76984610-11
ACATGGG97650.020.7187353
GAGTACT69950.020.119612-13
GTACTTT71200.019.76502414-15
TCTAACG4450.019.3962572
TAACGCC5000.019.0918834
Produced by FastQC (version 0.11.5)