FastQCFastQC Report
Sat 18 Jun 2016
SRR1931127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933029
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85770.29242806668464577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68370.2331037299665295No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41950.14302620260488388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.058.7821271
GTATCAA81800.044.7198451
ATCAACG106500.033.6213653
CAACGCA107150.033.5043335
TCAACGC109950.032.6522254
TATCAAC119000.030.568592
AACGCAG120800.030.347426
GTGGTAT18000.027.7487851
CGCAGAG130800.026.8655268
ACGCAGA141600.025.1182827
GACTGAC13050.023.2884657
GCAGAGT148150.022.9820589
GTACATG110250.022.1774581
GTACTAG9850.021.732191
ACATGGG107700.021.1650413
CAGAGTA140250.020.67065210-11
GTACTTT86400.020.50059514-15
AGAGTAC134600.020.47993510-11
GAGTACT88500.020.17511212-13
TACATGG121050.020.1254772
Produced by FastQC (version 0.11.5)