##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931127_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2933029 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.289810636035305 31.0 31.0 34.0 30.0 34.0 2 31.3005265205356 31.0 31.0 34.0 28.0 34.0 3 31.448656320820557 31.0 31.0 34.0 28.0 34.0 4 35.08134525775231 35.0 35.0 37.0 32.0 37.0 5 34.9614497504116 35.0 35.0 37.0 32.0 37.0 6 35.09309693153392 36.0 35.0 37.0 32.0 37.0 7 34.9460121260308 36.0 35.0 37.0 32.0 37.0 8 34.98810206104338 36.0 35.0 37.0 32.0 37.0 9 36.383340226093914 38.0 35.0 39.0 32.0 39.0 10-11 36.2662155744113 38.0 35.0 39.0 32.0 39.0 12-13 36.185453843108945 38.0 35.0 39.0 32.0 39.0 14-15 37.11325049973935 39.0 36.0 40.0 31.0 41.0 16-17 37.165365565768354 39.0 36.0 40.0 31.5 41.0 18-19 37.203682609343446 39.0 36.0 40.0 31.0 41.0 20-21 37.16740628885701 39.0 36.0 40.0 31.0 41.0 22-23 37.05358965083536 39.0 36.0 40.0 31.0 41.0 24-25 36.89452644348215 39.0 36.0 40.0 31.0 41.0 26-27 36.62537141637536 38.5 35.0 40.0 30.0 41.0 28-29 36.34711726341608 38.0 35.0 40.0 30.0 41.0 30-31 36.13509361823562 38.0 35.0 40.0 29.5 41.0 32-33 35.969306304165414 38.0 34.5 40.0 29.5 41.0 34-35 35.7206198779487 38.0 34.0 40.0 28.5 41.0 36-37 36.00970924597063 38.0 35.0 40.0 30.0 41.0 38-39 35.97705921080221 38.0 35.0 40.0 29.5 41.0 40-41 35.81323249787165 38.0 34.5 40.0 29.0 41.0 42-43 35.634971389645315 38.0 34.0 40.0 28.0 41.0 44-45 35.37186352402244 38.0 34.0 40.0 27.0 41.0 46-47 35.13364767276423 38.0 34.0 40.0 26.5 41.0 48-49 34.84205355623828 38.0 33.0 40.0 26.0 41.0 50-51 34.45670755386326 38.0 33.0 40.0 24.0 41.0 52-53 34.3100259151887 37.5 33.0 40.0 24.0 41.0 54-55 34.04843218392999 37.0 33.0 40.0 24.0 41.0 56-57 33.802205842492526 37.0 32.0 40.0 23.0 41.0 58-59 33.522271685687386 36.5 32.0 40.0 23.0 41.0 60-61 33.19784802673277 36.0 31.5 39.0 22.0 41.0 62-63 32.90928439507417 36.0 31.5 39.0 21.5 40.5 64-65 32.64344334815646 35.0 31.0 39.0 22.0 40.0 66-67 32.37365944898602 35.0 31.0 39.0 21.0 40.0 68-69 32.03970400565422 35.0 31.0 38.0 20.0 40.0 70-71 31.19992454899014 34.5 30.0 37.5 15.5 40.0 72-73 31.172461813367683 34.0 30.0 37.0 18.0 39.0 74-75 30.869304906293117 34.0 30.0 36.5 17.5 39.0 76-77 30.164960694217477 34.0 29.0 36.0 15.5 39.0 78-79 30.201947031549977 34.0 29.0 36.0 13.5 38.0 80-81 29.95426247064042 34.0 29.0 35.0 11.0 37.0 82-83 29.613826354938872 34.0 29.0 35.0 7.5 37.0 84-85 29.278911664357906 34.0 29.0 35.0 7.0 36.0 86-87 28.96850815317544 34.0 29.0 35.0 3.5 36.0 88-89 28.66855476028365 33.5 29.0 35.0 2.0 36.0 90-91 28.370658796759255 33.0 28.5 35.0 2.0 35.0 92-93 28.024043403594035 33.0 27.0 35.0 2.0 35.0 94-95 27.64408364185966 33.0 27.0 35.0 2.0 35.0 96-97 27.269261231307294 33.0 26.5 35.0 2.0 35.0 98-99 26.789046409019484 33.0 25.5 35.0 2.0 35.0 100-101 26.075838493243673 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7190493116896164 1101 2 0.7039517351872782 1101 3 0.46485756398454825 1101 4 0.7703812779438053 1101 5 0.7443357681120588 1101 6 0.6610238945936828 1101 7 0.7205891233428119 1101 8 0.6486437737949515 1101 9 0.992127596728146 1101 10-11 0.7978500215155009 1101 12-13 0.9589424169060337 1101 14-15 1.3511066993289234 1101 16-17 1.1438833979968166 1101 18-19 0.8748076413895518 1101 20-21 0.7363797657158884 1101 22-23 0.6829739529771146 1101 24-25 0.5379857015602667 1101 26-27 0.8479526291464623 1101 28-29 0.7634317709580003 1101 30-31 1.0277494510972005 1101 32-33 1.0072135771831725 1101 34-35 0.7870524965431542 1101 36-37 1.0487479366980068 1101 38-39 0.8357803348038644 1101 40-41 0.8662791237024265 1101 42-43 1.0677248554603267 1101 44-45 1.0095411410397332 1101 46-47 0.9579793557540413 1101 48-49 0.7063609634234496 1101 50-51 0.6706204856106694 1101 52-53 0.4732943846170272 1101 54-55 0.6311530821700373 1101 56-57 0.33646526814715827 1101 58-59 0.5815227181444342 1101 60-61 0.5981818791488038 1101 62-63 0.5088376876896561 1101 64-65 0.4766314351267269 1101 66-67 0.26085168473290565 1101 68-69 0.22971918970989336 1101 70-71 0.5563923090835843 1101 72-73 -0.0060675538983936406 1101 74-75 -0.3504323638099258 1101 76-77 -0.40077470120943204 1101 78-79 -0.3071488944921903 1101 80-81 -0.2662210259848976 1101 82-83 -0.36950966518608297 1101 84-85 -0.2703797164711439 1101 86-87 -0.2876793079281512 1101 88-89 -0.529276626193564 1101 90-91 -0.4859810993664837 1101 92-93 -0.7251764352567172 1101 94-95 -0.7331416220272722 1101 96-97 -0.7541550984701288 1101 98-99 -0.7653811472618273 1101 100-101 -1.006477104898412 1102 1 0.8652841114946916 1102 2 0.8162935001664842 1102 3 0.5991417240498258 1102 4 0.8665023241931991 1102 5 0.8959759158925209 1102 6 0.7664150764973812 1102 7 0.8904211987289727 1102 8 0.9364904175692459 1102 9 1.2037493002832633 1102 10-11 1.183065388303433 1102 12-13 1.2511624811633268 1102 14-15 1.647545419735998 1102 16-17 1.4495474184412913 1102 18-19 1.2147294021755997 1102 20-21 1.2222701458199197 1102 22-23 0.8613940676656284 1102 24-25 0.5462184557845902 1102 26-27 0.8724517876818041 1102 28-29 1.0747091681406502 1102 30-31 1.3608831136500399 1102 32-33 1.4723073140795577 1102 34-35 1.2393982563545691 1102 36-37 1.449513187890787 1102 38-39 1.3760493060070687 1102 40-41 1.438454904819622 1102 42-43 1.601146160098864 1102 44-45 1.6068377587484548 1102 46-47 1.6788595079543214 1102 48-49 1.3428989454948734 1102 50-51 1.2869417834494996 1102 52-53 1.3034196693550726 1102 54-55 1.1594466230079874 1102 56-57 1.2172587664869 1102 58-59 1.3093363833038296 1102 60-61 1.58153929480401 1102 62-63 1.4707542580270356 1102 64-65 1.450002761864333 1102 66-67 1.1966173325756913 1102 68-69 1.1093146633666713 1102 70-71 1.4888447960791318 1102 72-73 1.0043340967322756 1102 74-75 0.9719154642479602 1102 76-77 0.9337106879042274 1102 78-79 0.9029971293748069 1102 80-81 0.8643894467085858 1102 82-83 0.878303447496517 1102 84-85 0.897630035452611 1102 86-87 1.0713850655348303 1102 88-89 0.8576366151169736 1102 90-91 0.9158528402747166 1102 92-93 0.78009645994835 1102 94-95 0.7835496227737799 1102 96-97 0.7958179859936543 1102 98-99 0.8498461048602515 1102 100-101 0.7652660443893176 1103 1 0.7001096001350327 1103 2 0.5387594382450231 1103 3 0.3514806284375638 1103 4 0.7059604047815284 1103 5 0.6634466742555034 1103 6 0.5685389876676439 1103 7 0.5638831867337188 1103 8 0.531007237375924 1103 9 0.9273426730320224 1103 10-11 0.7804430255962203 1103 12-13 0.8046684915887923 1103 14-15 1.2736453403932586 1103 16-17 0.9841756166786908 1103 18-19 0.938547579340252 1103 20-21 0.9165935143882535 1103 22-23 0.6918697828166742 1103 24-25 0.49805613590539366 1103 26-27 0.877404736586783 1103 28-29 0.5983816703375098 1103 30-31 1.1206190217830994 1103 32-33 1.2143139601000428 1103 34-35 1.031070468481218 1103 36-37 1.1682207971150191 1103 38-39 0.9327774419646815 1103 40-41 1.0193414105826335 1103 42-43 1.293680051390794 1103 44-45 1.1550939248733698 1103 46-47 1.2171389650111308 1103 48-49 1.0476279431316584 1103 50-51 0.8462018484593514 1103 52-53 0.41065393517882853 1103 54-55 0.59947055380065 1103 56-57 0.6314139796458491 1103 58-59 0.7776683942582423 1103 60-61 0.9664780387999841 1103 62-63 0.7733294843244636 1103 64-65 0.5953629181609941 1103 66-67 0.5056739218618631 1103 68-69 0.3969602316910006 1103 70-71 0.7046035003184237 1103 72-73 0.3259140409364214 1103 74-75 0.08776289584684704 1103 76-77 0.1921242171202664 1103 78-79 0.2804202820967774 1103 80-81 0.27063192834457794 1103 82-83 0.20285149250858936 1103 84-85 0.25943120085235094 1103 86-87 0.29545662756293467 1103 88-89 0.07640074537555819 1103 90-91 0.03044379861551505 1103 92-93 -0.31795132485367716 1103 94-95 -0.33929170028765654 1103 96-97 -0.4485958162296484 1103 98-99 -0.6211856919540963 1103 100-101 -0.7601270434081115 1104 1 0.5267864024176276 1104 2 0.4117778732642705 1104 3 0.21745394261041895 1104 4 0.6929508904927388 1104 5 0.4634105748015074 1104 6 0.5094523385647491 1104 7 0.5410556622884855 1104 8 0.5249761987876909 1104 9 0.9668569965317246 1104 10-11 0.7116671441968094 1104 12-13 0.6495475757734184 1104 14-15 1.105393334964461 1104 16-17 0.9436459960353574 1104 18-19 0.7800393892766238 1104 20-21 0.7401383419265812 1104 22-23 0.3713879418833699 1104 24-25 0.5363669417909946 1104 26-27 0.23671908993796364 1104 28-29 0.6212896375564227 1104 30-31 0.9150773217070807 1104 32-33 0.9232046655319763 1104 34-35 0.7527896702667505 1104 36-37 0.9531781719904941 1104 38-39 0.5559493864409504 1104 40-41 0.841906172655996 1104 42-43 1.0608339739088706 1104 44-45 1.021762500645778 1104 46-47 1.034235787912074 1104 48-49 0.8022571774414047 1104 50-51 0.6455865957458187 1104 52-53 0.41553256812027684 1104 54-55 0.7816068778349106 1104 56-57 0.6406041543463843 1104 58-59 0.5634770567387619 1104 60-61 0.8386633227187161 1104 62-63 0.06070500195244932 1104 64-65 -0.8822741643089067 1104 66-67 -0.1836318752244921 1104 68-69 0.35668998909572736 1104 70-71 0.879810551513696 1104 72-73 0.5006992665406536 1104 74-75 0.33780958215994517 1104 76-77 0.041460629320493325 1104 78-79 -0.04291515125287049 1104 80-81 0.12911894468999563 1104 82-83 0.0015811561935805685 1104 84-85 -0.14388908113992116 1104 86-87 0.19651340436764642 1104 88-89 0.32393374687160303 1104 90-91 0.29263508753957623 1104 92-93 0.31928257183940545 1104 94-95 0.32737227627546517 1104 96-97 0.34342108456280585 1104 98-99 0.2700207934516605 1104 100-101 -0.02564139120552511 1105 1 -0.07215868316491836 1105 2 -1.395549137074788 1105 3 -0.26338258121624847 1105 4 0.5072951442674452 1105 5 0.6341677475301992 1105 6 0.07527326462663808 1105 7 -0.027178458464803157 1105 8 0.014505727309547467 1105 9 0.7074117867579304 1105 10-11 0.9788414266503906 1105 12-13 0.943468906359584 1105 14-15 1.336831447782636 1105 16-17 0.9212801362653309 1105 18-19 0.7312442085537327 1105 20-21 1.016563887903061 1105 22-23 0.36221477660395607 1105 24-25 -0.160855687715312 1105 26-27 0.9476696946454126 1105 28-29 0.49210969920343217 1105 30-31 0.9079908430160017 1105 32-33 1.1398056713169922 1105 34-35 0.6528195144641771 1105 36-37 0.7579652797348828 1105 38-39 0.8203744465943785 1105 40-41 1.0936603330629424 1105 42-43 1.486270776974365 1105 44-45 1.292668046359644 1105 46-47 1.4530661634510977 1105 48-49 1.2257397189962376 1105 50-51 0.9692041610939341 1105 52-53 1.0967005239322631 1105 54-55 0.1436053941050588 1105 56-57 0.4568830288125483 1105 58-59 -0.3185935834644056 1105 60-61 0.460273722176872 1105 62-63 1.0438668291723303 1105 64-65 1.2355379797354402 1105 66-67 1.0579542768490668 1105 68-69 0.9457824683579723 1105 70-71 0.4041636354505691 1105 72-73 -0.1740789946286263 1105 74-75 -0.18466468508431078 1105 76-77 0.10840772931797815 1105 78-79 -0.32566331765383083 1105 80-81 -0.07753598307665044 1105 82-83 -0.061839596109972916 1105 84-85 0.04661067102466987 1105 86-87 -0.23736487507470727 1105 88-89 -0.49929658249234876 1105 90-91 -0.5664111509973537 1105 92-93 -0.22458787863017093 1105 94-95 -0.5127059095037048 1105 96-97 -0.6559273763957307 1105 98-99 -1.1224154121827965 1105 100-101 -0.528490157991861 1106 1 0.3515422823993788 1106 2 0.4424723222785012 1106 3 -0.21555276891896114 1106 4 0.4217189105278578 1106 5 0.25621182288846 1106 6 0.3417708753680415 1106 7 0.37556219304457983 1106 8 0.4919310313303811 1106 9 0.9344693075256885 1106 10-11 0.2762228365395032 1106 12-13 0.3724024548550062 1106 14-15 0.08518774980198174 1106 16-17 -0.16333866172304567 1106 18-19 0.5069983641047315 1106 20-21 0.5232745997235568 1106 22-23 0.5068998422052999 1106 24-25 0.3879905255829783 1106 26-27 0.871060176521425 1106 28-29 0.8621492892050568 1106 30-31 0.30405509581635215 1106 32-33 0.35357735520462086 1106 34-35 -1.0526124176505007 1106 36-37 -0.7798870792748716 1106 38-39 -0.7061472566151963 1106 40-41 0.5793021281650326 1106 42-43 0.855751363082284 1106 44-45 0.6013034905378731 1106 46-47 0.11786337629366272 1106 48-49 0.12858630807307492 1106 50-51 0.8014883349541435 1106 52-53 0.15238837189385634 1106 54-55 0.8184150396206249 1106 56-57 0.4816860782154393 1106 58-59 0.89693439871143 1106 60-61 1.1716594788376113 1106 62-63 0.9607161659631771 1106 64-65 0.7906035277724612 1106 66-67 0.5182733288211026 1106 68-69 0.6240164291437473 1106 70-71 1.0429258582472691 1106 72-73 0.32634775387425563 1106 74-75 0.4738835438261155 1106 76-77 0.5515449071411815 1106 78-79 0.5303552020985265 1106 80-81 0.6403053024590726 1106 82-83 0.48392743185487674 1106 84-85 0.5872200718844311 1106 86-87 0.6712489818256273 1106 88-89 0.658779591282574 1106 90-91 0.8389702775474248 1106 92-93 0.36489105511820696 1106 94-95 0.65703105884295 1106 96-97 0.8335101817366741 1106 98-99 0.7135006213014705 1106 100-101 0.6445801792227428 1107 1 0.4224709647763554 1107 2 0.39298926700347536 1107 3 0.16238230154799282 1107 4 -1.2632516422182505 1107 5 -0.019678795798959925 1107 6 0.19985226786356236 1107 7 0.41929821411498125 1107 8 0.38252331654537386 1107 9 0.7800150389318645 1107 10-11 0.7616632397331955 1107 12-13 0.8091369851847148 1107 14-15 0.9444146612850375 1107 16-17 0.8405784670511451 1107 18-19 0.6304621702086308 1107 20-21 -0.08170021154212037 1107 22-23 0.38692462092897273 1107 24-25 0.2714804377682256 1107 26-27 0.7422621423774842 1107 28-29 0.6853151956609196 1107 30-31 0.8478959750365362 1107 32-33 1.0194839922621668 1107 34-35 0.8634921323700766 1107 36-37 0.9469210469288853 1107 38-39 0.8582136714003994 1107 40-41 0.25185236119809673 1107 42-43 0.09770267825352619 1107 44-45 -0.10744164809231194 1107 46-47 0.3208399706914662 1107 48-49 0.6475375226053472 1107 50-51 -0.8724691871155059 1107 52-53 0.49304715959180356 1107 54-55 0.9968967339916617 1107 56-57 0.7634388136386789 1107 58-59 0.9738713935363918 1107 60-61 1.2400245075808058 1107 62-63 1.2326662303948979 1107 64-65 1.210012649639424 1107 66-67 0.9186423228641374 1107 68-69 1.0676071966981588 1107 70-71 1.5387469335395103 1107 72-73 0.9925862821182925 1107 74-75 0.8147531867420561 1107 76-77 0.8845935194933112 1107 78-79 0.8297921570613447 1107 80-81 0.9729755868138739 1107 82-83 0.8935627439589098 1107 84-85 0.703606871778323 1107 86-87 0.8965092661038092 1107 88-89 0.9819590094526269 1107 90-91 0.979529767088323 1107 92-93 0.9222579367781627 1107 94-95 0.8606194854022782 1107 96-97 1.0119201760151597 1107 98-99 0.908120479317688 1107 100-101 0.9100595044363473 1108 1 0.5611664542405208 1108 2 0.45117729627642333 1108 3 0.2853452129416105 1108 4 0.6436577208128185 1108 5 0.5479048998833136 1108 6 0.46067511591717647 1108 7 0.6180118742969398 1108 8 0.6106895337104277 1108 9 0.9223900922780999 1108 10-11 0.748948298723974 1108 12-13 0.8679889146685653 1108 14-15 1.1663236464604125 1108 16-17 0.8719471858796624 1108 18-19 0.7854247676714579 1108 20-21 0.710890044853663 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2311 58-59 1.4068750878305707 2311 60-61 1.797466442839621 2311 62-63 1.8767117973269691 2311 64-65 -1.0729881943817219 2311 66-67 1.0002705264434084 2311 68-69 1.609624049894638 2311 70-71 2.0862386507311648 2311 72-73 1.6358331344809578 2311 74-75 1.2421584343074095 2311 76-77 1.4007790288753874 2311 78-79 1.1341828112287828 2311 80-81 1.3089642827161185 2311 82-83 1.389740825614549 2311 84-85 1.5707798944120448 2311 86-87 1.648710570252618 2311 88-89 1.681973090652228 2311 90-91 1.8103165020027205 2311 92-93 1.8422199303016633 2311 94-95 1.8583390542708322 2311 96-97 1.767123625951605 2311 98-99 1.874398558761179 2311 100-101 1.9355101028235175 2312 1 -0.03957232850342152 2312 2 -0.07321675853255627 2312 3 -0.3191394743782432 2312 4 0.19941251362833867 2312 5 -1.0871840950986495 2312 6 -0.0437928940540786 2312 7 0.2634296583551148 2312 8 -2.57302198733516 2312 9 -4.723038675763782 2312 10-11 -0.4578189702494413 2312 12-13 -5.893549073713679 2312 14-15 -2.0992586447217008 2312 16-17 -3.0714908815689483 2312 18-19 -2.5833525080772404 2312 20-21 -3.8368474467229916 2312 22-23 -2.1385545128308934 2312 24-25 -2.7902742300450853 2312 26-27 -3.0353353607754485 2312 28-29 -5.731396217046303 2312 30-31 -6.348797085491803 2312 32-33 -1.5418541862480666 2312 34-35 0.655167622199663 2312 36-37 1.0036162610292436 2312 38-39 0.9462964773246583 2312 40-41 -2.3286712449042213 2312 42-43 -4.5774266621124085 2312 44-45 -3.2486445765180925 2312 46-47 0.4082951616283168 2312 48-49 -1.0712373938266992 2312 50-51 -6.529482053087701 2312 52-53 -1.3565949801224448 2312 54-55 0.9565373359546498 2312 56-57 1.451094344393347 2312 58-59 1.5328077810525897 2312 60-61 1.9821472237320918 2312 62-63 1.7444887285240327 2312 64-65 1.7790014107305083 2312 66-67 1.5130324298296856 2312 68-69 1.5344801813256872 2312 70-71 1.9812383678688867 2312 72-73 1.5683548336286357 2312 74-75 1.5355161058332847 2312 76-77 1.6868629270305213 2312 78-79 1.3569380583240296 2312 80-81 1.4278374556966327 2312 82-83 0.8498542353327387 2312 84-85 1.1980853724312404 2312 86-87 1.7299973649174731 2312 88-89 1.8509929307327297 2312 90-91 2.017347013814927 2312 92-93 2.21490181375518 2312 94-95 2.246188374137514 2312 96-97 2.331841311343755 2312 98-99 2.5045964540251013 2312 100-101 2.5357938076693713 2313 1 -0.7088303119314077 2313 2 -0.11289338702773222 2313 3 -0.6700223841011095 2313 4 -1.4473615297568188 2313 5 -2.6162471866791996 2313 6 -1.8339117532540357 2313 7 -2.6997953672477166 2313 8 -4.080082161744741 2313 9 -3.466094877855454 2313 10-11 -1.611366126007752 2313 12-13 -4.0256441610736395 2313 14-15 -4.137724957603808 2313 16-17 -7.077907106004915 2313 18-19 -4.81223405231583 2313 20-21 -5.2093143182123 2313 22-23 -2.810690875056629 2313 24-25 -1.3301680946759404 2313 26-27 -3.114673100898962 2313 28-29 -4.7797855662689095 2313 30-31 -3.6908896901530674 2313 32-33 -7.693298806550338 2313 34-35 -3.8483547065717048 2313 36-37 -3.4682594178336856 2313 38-39 -2.7254907553973027 2313 40-41 -1.7321485747617729 2313 42-43 -3.893109559421781 2313 44-45 -5.788197070078596 2313 46-47 -3.649955156467456 2313 48-49 -1.56482116939074 2313 50-51 0.0788024021151088 2313 52-53 -0.5386621371221025 2313 54-55 -4.405626335956718 2313 56-57 -6.493463890407888 2313 58-59 -1.5593003404755095 2313 60-61 -7.698849269456005 2313 62-63 -2.549602448585489 2313 64-65 -3.0891672886457187 2313 66-67 -4.356323309859356 2313 68-69 -6.555160758375763 2313 70-71 -4.62650299699472 2313 72-73 -0.8389939545040619 2313 74-75 0.41809997449852787 2313 76-77 -2.2511627068334867 2313 78-79 -0.2660175944705614 2313 80-81 0.5520111825377079 2313 82-83 1.1107858680174623 2313 84-85 1.0186560827934024 2313 86-87 1.2045186695756342 2313 88-89 -3.827381211960379 2313 90-91 0.5934262119032496 2313 92-93 1.150429232058876 2313 94-95 0.9234220271183027 2313 96-97 0.7756469781035236 2313 98-99 0.6184902565884869 2313 100-101 0.9622593757418656 2314 1 -28.774047703235755 2314 2 -14.836513491868928 2314 3 -4.851798517311103 2314 4 -3.334564736664003 2314 5 -1.1412548955906985 2314 6 -0.5859314880674731 2314 7 -0.030415163732456563 2314 8 -5.021124406579773 2314 9 -2.7517871381083054 2314 10-11 -0.5507586637814157 2314 12-13 0.5123145191607108 2314 14-15 -1.1241572733608223 2314 16-17 -0.8875432453820338 2314 18-19 -2.825138770169673 2314 20-21 -2.3450528319341615 2314 22-23 -5.2150849546126 2314 24-25 -1.021564949440048 2314 26-27 -2.913100482952622 2314 28-29 -1.8671275335831226 2314 30-31 -4.0301280496967 2314 32-33 -2.266780150984502 2314 34-35 -1.8444694151748493 2314 36-37 -5.174441964858044 2314 38-39 -7.3720217452215415 2314 40-41 -6.908266366929521 2314 42-43 -4.769227351589528 2314 44-45 -3.9461372489253392 2314 46-47 -6.915295890355829 2314 48-49 -5.977880065474608 2314 50-51 -2.206411905624641 2314 52-53 -3.1059609345319075 2314 54-55 -4.823973545330752 2314 56-57 -2.2270747445552814 2314 58-59 -1.3803302510521362 2314 60-61 -0.09167998175942671 2314 62-63 1.0235705393886079 2314 64-65 -0.9321213435331686 2314 66-67 -0.22849413933823115 2314 68-69 0.935403533946161 2314 70-71 1.3047489302331563 2314 72-73 1.0628253486103354 2314 74-75 0.7450503291084623 2314 76-77 -0.557977384600985 2314 78-79 -2.0056722352352807 2314 80-81 -0.5580874915295126 2314 82-83 -0.714406854770079 2314 84-85 0.6020202517727462 2314 86-87 1.2623663414843591 2314 88-89 1.3128438882554718 2314 90-91 1.9439966713543768 2314 92-93 1.7047616403228325 2314 94-95 1.9046739543461761 2314 96-97 2.250346330554958 2314 98-99 2.291879094084635 2314 100-101 2.6361415835741155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 19.0 4 66.0 5 136.0 6 291.0 7 766.0 8 2018.0 9 3487.0 10 5317.0 11 7756.0 12 10154.0 13 12505.0 14 15511.0 15 19139.0 16 22394.0 17 25145.0 18 22964.0 19 19245.0 20 18742.0 21 19781.0 22 21532.0 23 24480.0 24 29904.0 25 37126.0 26 46043.0 27 58800.0 28 73856.0 29 91621.0 30 112019.0 31 135669.0 32 163943.0 33 197335.0 34 241884.0 35 296431.0 36 369564.0 37 432364.0 38 331998.0 39 62986.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.81632443980614 21.2382592873886 14.763314336919523 23.182101935885736 2 15.475844255205114 21.37694513078459 39.313010543025655 23.834200070984636 3 18.52770036161889 26.15667924343027 30.64657021223203 24.669050182718806 4 13.274883908053814 17.609631028768984 37.89974156330335 31.21574349987385 5 12.901475986704494 38.111319352981106 35.095145926333295 13.892058733981107 6 30.40668878487052 38.2072253632678 17.54762738452296 13.838458467338713 7 26.946647987455975 33.00795866662075 22.223032912391933 17.822360433531344 8 26.048429797318747 35.26998880679325 19.96628741140984 18.715293984478162 9 25.974478943099438 15.793843156682053 20.875006691035104 37.3566712091834 10-11 24.708773762550592 26.504749867798783 28.26088661244059 20.52558975721004 12-13 25.194704859720108 24.321085812653063 28.283201427602663 22.201007900024173 14-15 22.760770520850627 25.71820803681109 25.98581534652402 25.53520609581426 16-17 22.165532628555667 28.61386641591338 26.578257494215023 22.64234346131593 18-19 21.984218362655124 27.594186760512766 28.804505512901507 21.6170893639306 20-21 22.924048824611006 26.71125992958133 28.648983695694792 21.71570755011287 22-23 22.786034505625413 26.41397340428615 28.584187200331122 22.21580488975731 24-25 22.532242554033132 26.818268620884854 28.47261343718243 22.17687538789958 26-27 22.51826251097933 26.81489669883633 28.411519566391526 22.25532122379282 28-29 22.26887511445624 26.807658454230886 28.69562593476502 22.227840496547856 30-31 22.645693469256454 26.51709531751223 28.575137620199786 22.262073593031527 32-33 22.611411220153506 26.802313529318706 28.292404930365006 22.29387032016278 34-35 22.692921335040577 26.707613511742885 28.57763384423981 22.02183130897673 36-37 22.74944979636925 26.504608754502634 28.35527044788536 22.390671001242755 38-39 22.69374071349034 26.632192506148954 28.523520823943123 22.150545956417588 40-41 22.43833151322835 26.569827762266446 28.632746001423126 22.35909472308208 42-43 22.552168357083726 26.706867771063386 28.30976364164522 22.431200230207665 44-45 22.607989597049315 26.816953481981333 28.10499711218562 22.47005980878373 46-47 22.809546424609728 26.506633061542228 27.995467400840866 22.688353113007178 48-49 22.95911416786828 26.77907171631766 27.762935591732642 22.498878524081427 50-51 22.73218418005316 26.96187333744066 27.378060213930254 22.92788226857592 52-53 22.69826105723547 26.987758903971343 27.342073222913555 22.971906815879635 54-55 22.852838549407366 27.060998555055487 27.27085913743651 22.815303758100633 56-57 22.864363155689343 26.960457906424175 27.15138862891625 23.02379030897023 58-59 22.763018935471084 27.108758561023528 27.327852573527846 22.800369929977542 60-61 22.789244722756102 27.134432100672264 27.457556846872556 22.618766329699074 62-63 22.766047872515436 27.061096297548993 27.257230852843506 22.915624977092065 64-65 22.559933659839942 27.040093754576528 27.35392744761042 23.04604513797311 66-67 22.918532727828804 26.883674733733603 27.090963087022917 23.106829451414672 68-69 22.690449896572172 26.895105734371693 27.172862392058818 23.24158197699732 70-71 22.75481850388687 26.715507061047195 27.343489084899808 23.186185350166124 72-73 22.86835700613249 26.682719027093306 27.395051045005935 23.05387292176827 74-75 22.631791770741064 27.199796931331015 27.109820736325084 23.058590561602838 76-77 22.67625038825051 27.05655143539324 26.939897287070806 23.327300889285446 78-79 22.498498991997693 27.132633192511907 26.918929202541126 23.449938612949275 80-81 22.560620111693748 27.058560800812813 27.07605130548036 23.304767782013077 82-83 22.60063835347558 26.8293593502521 27.291784612725234 23.27821768354708 84-85 22.858812998237145 26.920297569559924 26.8090985137035 23.411790918499428 86-87 22.418099949863286 27.251596115282727 27.013834561804963 23.31646937304902 88-89 22.703715712959394 26.667645212642814 27.08277714757599 23.54586192682181 90-91 22.64429813944132 26.71870684864251 27.161309789668564 23.475685222247606 92-93 22.66745381686586 26.888930333797827 26.98216433118543 23.46145151815088 94-95 22.38614581763556 26.688284988165723 27.087141996981934 23.83842719721678 96-97 22.571833907486354 26.6045258532514 26.94702313250252 23.87661710675973 98-99 22.211152712691845 26.922570087809966 27.059921789482814 23.806355410015374 100-101 22.55195498726111 26.758457149335268 26.868363651241626 23.821224212161997 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 427.0 1 302.0 2 196.5 3 249.5 4 230.5 5 231.0 6 440.5 7 841.0 8 1424.5 9 1774.5 10 1611.5 11 1421.5 12 1510.0 13 1719.0 14 1915.0 15 2172.0 16 2442.5 17 2742.0 18 3056.5 19 3367.0 20 3749.5 21 4198.0 22 4785.0 23 5643.5 24 6972.5 25 9061.5 26 12327.5 27 16004.0 28 19644.0 29 25102.5 30 32035.5 31 38508.5 32 46803.5 33 55906.0 34 64260.5 35 71860.5 36 80155.0 37 89191.5 38 93182.0 39 92788.5 40 90583.0 41 87862.0 42 86420.0 43 89297.0 44 95563.5 45 101704.5 46 103967.5 47 104493.0 48 105204.5 49 102883.5 50 100731.5 51 100578.0 52 101100.5 53 100919.0 54 100684.5 55 97361.0 56 89376.0 57 79521.5 58 71992.5 59 66635.5 60 58303.0 61 51127.0 62 42950.0 63 30898.0 64 20913.0 65 14494.5 66 10221.5 67 7034.0 68 5035.0 69 3781.0 70 2966.5 71 2229.0 72 1644.0 73 1206.5 74 861.0 75 656.5 76 481.5 77 306.5 78 212.0 79 181.0 80 149.5 81 112.5 82 87.0 83 69.0 84 52.0 85 41.5 86 35.0 87 23.0 88 15.5 89 10.5 90 6.0 91 4.5 92 2.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16218728147590766 2 0.0 3 0.0036481057637002563 4 3.068500175074982E-4 5 2.727555711177762E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.01939973999575183 26-27 0.008353139365481895 28-29 0.0047561752713662225 30-31 0.0016194862035117962 32-33 7.159833741841625E-4 34-35 0.00134673063239402 36-37 8.182667133533286E-4 38-39 3.750389102869423E-4 40-41 0.0016365334267066571 42-43 8.523611597430505E-4 44-45 5.114166958458304E-4 46-47 0.03192944904397468 48-49 0.01737112043556337 50-51 0.08504859651916159 52-53 0.001363777855588881 54-55 0.03690723821687409 56-57 9.546444989122166E-4 58-59 0.0014149195251734641 60-61 0.0036140113173105345 62-63 0.0031878307374390096 64-65 0.009052075516471199 66-67 0.00538692252957608 68-69 0.0022502334617216535 70-71 0.004858458610535389 72-73 0.004329994691494698 74-75 0.0010569278380813828 76-77 0.0 78-79 0.0 80-81 3.068500175074982E-4 82-83 4.943694726509694E-4 84-85 1.704722319486101E-5 86-87 0.0016535806499015182 88-89 0.0036140113173105345 90-91 0.0013296834091991588 92-93 0.0029321223895160944 94-95 7.841722669636066E-4 96-97 4.432278030663863E-4 98-99 0.0016194862035117962 100-101 0.002778697380762345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2933029.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.79708698412026 #Duplication Level Percentage of deduplicated Percentage of total 1 73.02541309438166 32.7132578244033 2 13.994300403903233 12.53807784951125 3 5.128036838955839 6.891633369974334 4 2.407073209746525 4.313194718166426 5 1.3192707692204537 2.954974370218795 6 0.8326620387238467 2.2380500266252246 7 0.5555483252271266 1.7420862654357345 8 0.3858987011743223 1.3829710146852117 9 0.2790359493227589 1.1249997924157382 >10 1.7496335243793064 14.977690406096219 >50 0.19061632060553765 5.998206303450123 >100 0.12598840900705108 10.246694625618403 >500 0.0052024027213975425 1.4870487883339625 >1k 0.0011647170271629039 0.8549547886146341 >5k 1.5529560362172052E-4 0.5361598564508715 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8577 0.29242806668464577 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6837 0.2331037299665295 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4195 0.14302620260488388 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.704722319486101E-5 0.0 20-21 0.0 0.0 0.0 1.1933056236402707E-4 0.0 22-23 0.0 0.0 0.0 2.557083479229152E-4 0.0 24-25 0.0 0.0 0.0 5.455111422355524E-4 0.0 26-27 0.0 0.0 0.0 0.0012444472932248538 0.0 28-29 0.0 0.0 0.0 0.003614011317310535 0.0 30-31 0.0 0.0 0.0 0.011472781210141461 0.0 32-33 0.0 0.0 0.0 0.0279403988163772 0.0 34-35 0.0 0.0 0.0 0.05494320035703704 0.0 36-37 0.0 0.0 0.0 0.09602700825665209 0.0 38-39 0.0 0.0 0.0 0.1518566642198219 0.0 40-41 0.0 0.0 0.0 0.21249363712394254 0.0 42-43 0.0 0.0 0.0 0.2740511600805856 0.0 44-45 0.0 0.0 0.0 0.35146260060844947 0.0 46-47 0.0 0.0 0.0 0.44428473090446774 0.0 48-49 0.0 0.0 0.0 0.5409254391961348 0.0 50-51 0.0 0.0 0.0 0.6350602056781574 0.0 52-53 0.0 0.0 0.0 0.731138355604394 0.0 54-55 0.0 0.0 0.0 0.839933734033997 0.0 56-57 0.0 0.0 0.0 0.9648728328291333 0.0 58-59 0.0 0.0 0.0 1.1040804574383682 0.0 60-61 0.0 0.0 0.0 1.2441063487609567 0.0 62-63 1.704722319486101E-5 0.0 0.0 1.388189479203922 0.0 64-65 3.409444638972202E-5 0.0 0.0 1.5391767350408059 0.0 66-67 3.409444638972202E-5 0.0 0.0 1.7070407418406024 0.0 68-69 3.409444638972202E-5 0.0 0.0 1.8956341720453498 0.0 70-71 3.409444638972202E-5 1.704722319486101E-5 0.0 2.101121400436204 0.0 72-73 3.409444638972202E-5 3.409444638972202E-5 0.0 2.316035743253817 0.0 74-75 3.409444638972202E-5 3.409444638972202E-5 0.0 2.5308989444018453 0.0 76-77 3.409444638972202E-5 3.409444638972202E-5 0.0 2.765400546670353 0.0 78-79 3.409444638972202E-5 3.409444638972202E-5 0.0 3.029240420057217 0.0 80-81 3.409444638972202E-5 3.409444638972202E-5 0.0 3.3171509725952246 0.0 82-83 3.409444638972202E-5 3.409444638972202E-5 0.0 3.6071412863630057 0.0 84-85 3.409444638972202E-5 3.409444638972202E-5 0.0 3.89483022499948 0.0 86-87 3.409444638972202E-5 3.409444638972202E-5 0.0 4.203623625951192 0.0 88-89 3.409444638972202E-5 3.409444638972202E-5 0.0 4.538959553417303 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4200 0.0 58.782127 1 GTATCAA 8180 0.0 44.719845 1 ATCAACG 10650 0.0 33.621365 3 CAACGCA 10715 0.0 33.504333 5 TCAACGC 10995 0.0 32.652225 4 TATCAAC 11900 0.0 30.56859 2 AACGCAG 12080 0.0 30.34742 6 GTGGTAT 1800 0.0 27.748785 1 CGCAGAG 13080 0.0 26.865526 8 ACGCAGA 14160 0.0 25.118282 7 GACTGAC 1305 0.0 23.288465 7 GCAGAGT 14815 0.0 22.982058 9 GTACATG 11025 0.0 22.177458 1 GTACTAG 985 0.0 21.73219 1 ACATGGG 10770 0.0 21.165041 3 CAGAGTA 14025 0.0 20.670652 10-11 GTACTTT 8640 0.0 20.500595 14-15 AGAGTAC 13460 0.0 20.479935 10-11 GAGTACT 8850 0.0 20.175112 12-13 TACATGG 12105 0.0 20.125477 2 >>END_MODULE