FastQCFastQC Report
Sat 18 Jun 2016
SRR1931126_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931126_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410938
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50410.2090887447126388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33980.1409409947497613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26250.053.12451
GTATCAA53750.042.4708251
ATCAACG62050.034.6595843
CAACGCA63150.034.3595965
TATCAAC67000.033.572792
TCAACGC64950.033.41064
AACGCAG66100.033.3798186
ACGCAGA75600.028.6275047
CGCAGAG77200.027.2936948
GTACATG61750.025.444871
GCAGAGT83100.024.2111389
TACATGG65400.023.5136152
ACATGGG62300.023.345353
TAGTACT10600.021.5966994
GAGTACT46650.020.84534512-13
AGAGTAC73650.020.73042110-11
CAGAGTA82300.019.76524210-11
GTGGTAT14850.018.9743481
CATGGGG42400.017.5473184
GTACTTT56250.017.44828414-15
Produced by FastQC (version 0.11.5)