##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931126_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2410938 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.71225224373252 31.0 28.0 31.0 23.0 34.0 2 28.447201047890903 31.0 28.0 33.0 16.0 34.0 3 28.463895380138354 31.0 28.0 33.0 16.0 34.0 4 31.668904384932336 35.0 32.0 37.0 19.0 37.0 5 31.715844206694655 35.0 32.0 37.0 19.0 37.0 6 31.975673368622502 35.0 32.0 37.0 21.0 37.0 7 31.696253906156027 35.0 32.0 37.0 18.0 37.0 8 31.832389302420882 35.0 32.0 37.0 19.0 37.0 9 32.83892037041185 37.0 32.0 39.0 17.0 39.0 10-11 32.8037193407711 37.0 32.0 39.0 18.0 39.0 12-13 32.88941461787901 37.0 32.0 39.0 18.5 39.0 14-15 33.57619482541649 38.0 32.0 40.0 17.5 41.0 16-17 33.53976253225923 38.0 32.0 40.0 17.5 41.0 18-19 33.58957799827287 38.0 32.0 40.0 17.5 41.0 20-21 33.73820230964048 38.0 32.0 40.0 19.0 41.0 22-23 33.639061643227656 38.0 32.0 40.0 19.0 41.0 24-25 33.52616243138563 38.0 32.0 40.0 18.5 41.0 26-27 33.135999764407046 37.0 31.0 40.0 17.5 41.0 28-29 33.08131275047305 37.0 31.0 40.0 17.0 41.0 30-31 32.855795752524536 37.0 30.5 40.0 16.5 41.0 32-33 32.64083709328071 37.0 30.0 40.0 16.0 41.0 34-35 32.6419930748945 37.0 30.0 40.0 16.0 41.0 36-37 32.288959110520466 36.5 30.0 40.0 14.0 41.0 38-39 32.09029514653633 36.0 30.0 40.0 11.5 41.0 40-41 31.948298131266753 36.5 30.0 40.0 9.0 41.0 42-43 32.149756443342795 37.0 30.0 40.0 9.0 41.0 44-45 32.23117786521263 37.0 30.0 40.0 9.0 41.0 46-47 31.991132496978352 37.0 30.0 40.0 8.5 41.0 48-49 31.747788620030875 36.0 30.0 40.0 7.5 41.0 50-51 31.225835338776857 35.5 29.5 39.5 6.0 40.5 52-53 31.14626133065222 36.0 29.0 39.0 2.0 40.5 54-55 31.128843006331977 36.0 29.0 40.0 2.0 41.0 56-57 30.870925133703146 35.5 29.0 40.0 2.0 41.0 58-59 30.714040966627927 35.0 28.5 39.0 2.0 41.0 60-61 30.550589023857103 35.0 28.0 39.0 2.0 41.0 62-63 30.379729798111775 35.0 28.5 39.0 2.0 40.0 64-65 30.007582111194896 34.5 28.0 39.0 2.0 40.0 66-67 29.78955389976847 34.0 28.0 38.0 2.0 40.0 68-69 29.583476223776806 34.0 28.0 38.0 2.0 40.0 70-71 29.261781306694736 34.0 28.0 37.5 2.0 40.0 72-73 28.91810780700292 34.0 27.5 37.0 2.0 40.0 74-75 28.495047155920226 34.0 26.5 36.5 2.0 39.0 76-77 28.181232159433385 33.5 26.0 36.0 2.0 39.0 78-79 27.770832555627727 33.0 26.0 35.5 2.0 38.5 80-81 27.36465869300662 33.0 26.0 35.0 2.0 37.0 82-83 27.02328886101592 33.0 26.0 35.0 2.0 37.0 84-85 26.682127661515977 32.5 25.0 35.0 2.0 36.5 86-87 26.364249101387095 32.0 25.0 35.0 2.0 36.0 88-89 26.02082695614736 32.0 24.0 35.0 2.0 36.0 90-91 25.654206578518405 32.0 23.0 35.0 2.0 35.0 92-93 25.289498734517437 32.0 20.0 35.0 2.0 35.0 94-95 24.97802680948245 32.0 19.0 34.5 2.0 35.0 96-97 24.582862147429758 32.0 13.5 34.0 2.0 35.0 98-99 24.19328472984374 32.0 2.0 34.0 2.0 35.0 100-101 23.598800549827494 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.29991928437029 1101 2 5.859314433221087 1101 3 5.4692003771666435 1101 4 6.760570858707485 1101 5 6.3499107609090615 1101 6 5.746870156787928 1101 7 6.418896138696443 1101 8 5.795411089312626 1101 9 6.52332179817396 1101 10-11 6.525154289429089 1101 12-13 6.310376782924102 1101 14-15 6.605102211226825 1101 16-17 6.525578219679691 1101 18-19 6.587566397294637 1101 20-21 5.875474105281903 1101 22-23 5.914574929622766 1101 24-25 5.943126655107715 1101 26-27 5.9036898596319745 1101 28-29 5.800285961295057 1101 30-31 5.967839061392606 1101 32-33 5.7894018563858225 1101 34-35 5.695573817403314 1101 36-37 5.72503949231967 1101 38-39 5.608747362984126 1101 40-41 5.777650990553159 1101 42-43 5.701251715666853 1101 44-45 5.656588870954696 1101 46-47 5.455018599497436 1101 48-49 5.403297921881219 1101 50-51 5.431648513276905 1101 52-53 5.064545498079557 1101 54-55 5.399414632764479 1101 56-57 5.287951951948532 1101 58-59 5.357863173771257 1101 60-61 5.2542556229654025 1101 62-63 5.225558989151445 1101 64-65 5.2202313185098 1101 66-67 5.044093341203894 1101 68-69 5.19499106752987 1101 70-71 5.01315434325571 1101 72-73 4.753436326044461 1101 74-75 4.843409458688779 1101 76-77 4.738141818032577 1101 78-79 4.7435063176632575 1101 80-81 4.650132159204951 1101 82-83 4.554313992176368 1101 84-85 4.583305152712821 1101 86-87 4.491303865232979 1101 88-89 4.649356295972858 1101 90-91 4.582190768557826 1101 92-93 4.463418387520111 1101 94-95 4.479060457998514 1101 96-97 4.422120781115684 1101 98-99 4.0972947250818486 1101 100-101 4.037718901964503 1102 1 5.440001640345201 1102 2 5.963921648326746 1102 3 5.681521726329674 1102 4 6.989470678189885 1102 5 6.662318383838233 1102 6 6.08545509917748 1102 7 6.654334332646808 1102 8 6.227576666137271 1102 9 6.9217858738892915 1102 10-11 6.918637959052539 1102 12-13 6.937973603216378 1102 14-15 7.2466631997841695 1102 16-17 7.178047384666296 1102 18-19 7.2006587110584235 1102 20-21 6.458701158016069 1102 22-23 6.558703385010638 1102 24-25 6.392529809638571 1102 26-27 6.599167834071359 1102 28-29 6.470643914283553 1102 30-31 6.811995988009446 1102 32-33 6.523081657435419 1102 34-35 6.539650000824125 1102 36-37 6.612705429621766 1102 38-39 6.389413839946847 1102 40-41 6.6014038665408314 1102 42-43 6.682160328390847 1102 44-45 6.680066375634436 1102 46-47 6.6165821380594 1102 48-49 6.222072867762691 1102 50-51 6.270768424950411 1102 52-53 5.943769132181206 1102 54-55 6.304553314285943 1102 56-57 6.098130249501441 1102 58-59 6.344722257711812 1102 60-61 6.153177286896586 1102 62-63 6.040890777226458 1102 64-65 6.168267233750608 1102 66-67 6.0195804575036505 1102 68-69 6.0542498908612075 1102 70-71 5.970053922332934 1102 72-73 5.749054899982006 1102 74-75 5.765433815194438 1102 76-77 5.585138335337188 1102 78-79 5.469390386592821 1102 80-81 5.445510760610819 1102 82-83 5.3771719948148835 1102 84-85 5.361986983810045 1102 86-87 5.283754678735015 1102 88-89 5.3434930159402825 1102 90-91 5.302566942194584 1102 92-93 5.3506487077213585 1102 94-95 5.307527636775472 1102 96-97 5.223259202841099 1102 98-99 4.959384772875669 1102 100-101 4.951490042386304 1103 1 5.061316185265039 1103 2 5.59341688975935 1103 3 5.250746736091763 1103 4 6.743795598202329 1103 5 6.313218702265345 1103 6 5.556721284863372 1103 7 6.268337823107924 1103 8 5.638122150576198 1103 9 6.123528277274886 1103 10-11 6.241288671014587 1103 12-13 6.234201035269592 1103 14-15 6.552669332137722 1103 16-17 6.366513584790585 1103 18-19 6.244530697369491 1103 20-21 5.691549252434825 1103 22-23 5.831223160343363 1103 24-25 5.553447234016691 1103 26-27 5.7303875717328125 1103 28-29 5.59897182608999 1103 30-31 5.761783183385656 1103 32-33 5.554364959504689 1103 34-35 5.6379798520074615 1103 36-37 5.614565755259743 1103 38-39 5.369978576274534 1103 40-41 5.5606196259237315 1103 42-43 5.35228439426373 1103 44-45 5.25489739998952 1103 46-47 5.4002641280291 1103 48-49 5.010763436788807 1103 50-51 4.9292412068545985 1103 52-53 4.8120623334185275 1103 54-55 5.1767603220610745 1103 56-57 5.192701684242866 1103 58-59 5.287673123457374 1103 60-61 4.967468309021228 1103 62-63 4.914041627260879 1103 64-65 4.865382100258568 1103 66-67 5.115178880463702 1103 68-69 5.05736752528459 1103 70-71 5.024151114221603 1103 72-73 4.826503170311323 1103 74-75 4.871682406172962 1103 76-77 4.906550751501388 1103 78-79 4.843977199685398 1103 80-81 4.781565941777732 1103 82-83 4.613427717359009 1103 84-85 4.624479977172033 1103 86-87 4.6372305032388255 1103 88-89 4.625771539672822 1103 90-91 4.559348814873186 1103 92-93 4.588678819286127 1103 94-95 4.563151386391166 1103 96-97 4.474536176636978 1103 98-99 4.253384007974571 1103 100-101 4.115597475728961 1104 1 4.567146474342561 1104 2 5.302125586881818 1104 3 4.958649222025166 1104 4 6.374485336040575 1104 5 5.951909446160059 1104 6 5.528914272103414 1104 7 6.160007189658362 1104 8 5.5961823281439145 1104 9 6.318447702558871 1104 10-11 6.157264082277187 1104 12-13 6.0852837972825 1104 14-15 6.080830779591029 1104 16-17 5.993665988267612 1104 18-19 6.222224017824026 1104 20-21 5.557041885897114 1104 22-23 5.787397738043133 1104 24-25 5.4399904884487675 1104 26-27 5.6254295162998105 1104 28-29 5.422525914503044 1104 30-31 5.751157911159684 1104 32-33 5.658026653623274 1104 34-35 5.564488459640117 1104 36-37 5.649379735440387 1104 38-39 5.44558236917414 1104 40-41 5.716752175114191 1104 42-43 5.6235668993610055 1104 44-45 5.639352289545943 1104 46-47 5.540487340550499 1104 48-49 5.320084740753941 1104 50-51 5.172846418744292 1104 52-53 4.970243648610033 1104 54-55 5.3060546883638615 1104 56-57 5.159657244555735 1104 58-59 5.169631459080438 1104 60-61 5.010209936974498 1104 62-63 4.9288775450616455 1104 64-65 5.15585014279365 1104 66-67 5.124362925709548 1104 68-69 4.9740520926933485 1104 70-71 4.849463194542615 1104 72-73 4.859951523733287 1104 74-75 4.823601238855492 1104 76-77 4.804227129164225 1104 78-79 4.698264576878053 1104 80-81 4.67523592480887 1104 82-83 4.368171448771129 1104 84-85 4.411064253848405 1104 86-87 4.312285970339225 1104 88-89 4.3515571537119015 1104 90-91 4.395376235959848 1104 92-93 4.250557845584471 1104 94-95 4.164781389731132 1104 96-97 4.147418997649336 1104 98-99 3.8983676882329767 1104 100-101 3.9268282066663147 1105 1 4.39168858727443 1105 2 4.984428240092733 1105 3 4.7192108878642145 1105 4 6.093435282941773 1105 5 3.399157097214683 1105 6 4.725525618053034 1105 7 5.566874463159262 1105 8 1.2568330949468738 1105 9 0.5136838265164663 1105 10-11 1.959018887822257 1105 12-13 5.279639060722644 1105 14-15 3.432672076783245 1105 16-17 5.50287559580396 1105 18-19 6.005212265013078 1105 20-21 3.0052491892306605 1105 22-23 5.132825910916296 1105 24-25 5.254889719196573 1105 26-27 5.524443706820925 1105 28-29 2.978613514549526 1105 30-31 5.204085644611094 1105 32-33 5.387079426814736 1105 34-35 5.357451372444437 1105 36-37 5.413247133484131 1105 38-39 5.366963815107013 1105 40-41 5.388295574775395 1105 42-43 2.761791917234028 1105 44-45 4.944262639504945 1105 46-47 5.29025251804967 1105 48-49 5.027374115263896 1105 50-51 5.158710485186781 1105 52-53 5.011289193833971 1105 54-55 5.2190584665905 1105 56-57 5.112920315231722 1105 58-59 5.112705708069452 1105 60-61 5.051553520600542 1105 62-63 4.706568943248826 1105 64-65 4.777319633157269 1105 66-67 4.88925077471367 1105 68-69 4.953864977467518 1105 70-71 4.8210013435541335 1105 72-73 4.725823709473097 1105 74-75 4.749870463356945 1105 76-77 4.541381430710956 1105 78-79 4.5627167180065555 1105 80-81 4.576918309807635 1105 82-83 4.429765155419254 1105 84-85 4.412390651201566 1105 86-87 4.403932186685843 1105 88-89 4.403699338744154 1105 90-91 4.418236956393809 1105 92-93 4.478144566947538 1105 94-95 4.434875610078489 1105 96-97 4.33645103077453 1105 98-99 4.2287479473269265 1105 100-101 4.336127939683582 1106 1 4.274204725521564 1106 2 4.495454511974184 1106 3 4.6302945783635465 1106 4 6.232705715682702 1106 5 6.1894023756609755 1106 6 5.2644243332577645 1106 7 6.051326224539796 1106 8 5.616286386655286 1106 9 6.1201364763821005 1106 10-11 5.956247222406702 1106 12-13 6.187685828974445 1106 14-15 5.968073446883114 1106 16-17 5.890596781877871 1106 18-19 6.1834516111567766 1106 20-21 5.517240516298777 1106 22-23 5.544287082291895 1106 24-25 5.563364621282435 1106 26-27 5.691962775064233 1106 28-29 5.699920618600014 1106 30-31 5.885792409447841 1106 32-33 5.6378976242209475 1106 34-35 5.253535021777054 1106 36-37 5.382631089705658 1106 38-39 5.364250033672199 1106 40-41 5.515630738774636 1106 42-43 2.577583003673748 1106 44-45 4.646714994402121 1106 46-47 4.874206699138114 1106 48-49 4.789957187009605 1106 50-51 4.9582429029007855 1106 52-53 4.842372032361293 1106 54-55 5.167605263659002 1106 56-57 5.1102074645226345 1106 58-59 4.929773392234118 1106 60-61 4.829643628453752 1106 62-63 4.8445618833362225 1106 64-65 5.006178359373198 1106 66-67 5.107015572447281 1106 68-69 5.122552822440113 1106 70-71 5.052242906614918 1106 72-73 4.7694141414694435 1106 74-75 4.923027939644022 1106 76-77 4.778041499964111 1106 78-79 4.84629610300447 1106 80-81 4.828256675503376 1106 82-83 4.871852859136318 1106 84-85 4.902256945291565 1106 86-87 4.829550125923056 1106 88-89 4.87814561958205 1106 90-91 4.909007880092556 1106 92-93 4.914152233263753 1106 94-95 4.855368006944236 1106 96-97 4.861551629696535 1106 98-99 4.625737586559737 1106 100-101 4.742763047588699 1107 1 3.2151372426458664 1107 2 0.03920061518173057 1107 3 1.0441579928320301 1107 4 2.8978756558868746 1107 5 2.7931877914424312 1107 6 4.010094582519127 1107 7 4.942412704239732 1107 8 4.494677681363651 1107 9 4.847426960500073 1107 10-11 2.5706236152147 1107 12-13 4.8566806817307295 1107 14-15 3.413298419702098 1107 16-17 2.1713060487105516 1107 18-19 2.7440340642871064 1107 20-21 2.442342138697949 1107 22-23 4.171375640728847 1107 24-25 4.318482244814415 1107 26-27 3.2850259736387812 1107 28-29 3.9096873247890294 1107 30-31 3.582404062367072 1107 32-33 3.9871536468548854 1107 34-35 4.4097069989861595 1107 36-37 4.467855853384595 1107 38-39 4.470146900571919 1107 40-41 3.361815136792334 1107 42-43 3.7720251643130744 1107 44-45 4.161678053840973 1107 46-47 4.4613552635019715 1107 48-49 2.3244131067619875 1107 50-51 3.7115612504219015 1107 52-53 3.7481730018309705 1107 54-55 4.4091439415696065 1107 56-57 3.169050121840801 1107 58-59 2.3801916579507783 1107 60-61 3.3287478758484 1107 62-63 3.9428576125524266 1107 64-65 4.297915111142277 1107 66-67 4.370747559660572 1107 68-69 4.305270572492233 1107 70-71 4.071694850307889 1107 72-73 3.9655093239858736 1107 74-75 3.9985176175750574 1107 76-77 3.8805793665456143 1107 78-79 3.8455259783349334 1107 80-81 3.892845983438793 1107 82-83 3.7551356327018226 1107 84-85 3.698846130045407 1107 86-87 3.686245922217573 1107 88-89 3.8523573078824 1107 90-91 3.834207836691199 1107 92-93 3.890252082097703 1107 94-95 3.9676199013645785 1107 96-97 3.915408695133486 1107 98-99 3.8639297837745836 1107 100-101 4.022344589041076 1108 1 -2.1598558729088424 1108 2 -1.4469648065533427 1108 3 -2.1389389678297803 1108 4 -3.7673871660398603 1108 5 -8.174648080157894 1108 6 -6.823684683761545 1108 7 -5.594608014112168 1108 8 -5.766579965072193 1108 9 -7.644434752016775 1108 10-11 -3.484520724401097 1108 12-13 -2.0627189865800517 1108 14-15 -6.6476903773912035 1108 16-17 -5.813195150727733 1108 18-19 -4.161722312589866 1108 20-21 -4.717108868096762 1108 22-23 -3.591163721506895 1108 24-25 -8.18421605474775 1108 26-27 -9.064338669300973 1108 28-29 -6.115054364107117 1108 30-31 -5.7785689643091125 1108 32-33 -2.7181315787359566 1108 34-35 -1.1697814108319058 1108 36-37 -4.5948333009476094 1108 38-39 -5.5097545490974795 1108 40-41 -3.2794433371202523 1108 42-43 -3.9491689732828767 1108 44-45 -1.8182740384796396 1108 46-47 -2.471470953755272 1108 48-49 -1.5768153200730808 1108 50-51 -0.846992333667334 1108 52-53 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-5.63137417573812 2313 56-57 -4.638259896350252 2313 58-59 -5.062869110006744 2313 60-61 -3.613737251734811 2313 62-63 -5.849434542350146 2313 64-65 -6.942829574939108 2313 66-67 -9.331162910848612 2313 68-69 -8.57847465440431 2313 70-71 -8.032479669104095 2313 72-73 -6.02520668250391 2313 74-75 -6.741559243730439 2313 76-77 -5.713499936622252 2313 78-79 -6.895407173634123 2313 80-81 -7.8490356435954105 2313 82-83 -6.821875216377972 2313 84-85 -6.997398072580005 2313 86-87 -5.83530679087179 2313 88-89 -7.737106629579703 2313 90-91 -7.841819302396626 2313 92-93 -7.281538671601963 2313 94-95 -7.924821387414326 2313 96-97 -8.848881935653647 2313 98-99 -7.765728601318582 2313 100-101 -6.729101681792599 2314 1 -11.431531774778108 2314 2 -12.88080473481766 2314 3 -13.62406301077922 2314 4 -24.825833352782556 2314 5 -18.621880968208846 2314 6 -16.29195543997519 2314 7 -18.0384610479337 2314 8 -13.484925445954175 2314 9 -14.100830034016179 2314 10-11 -13.84230818445214 2314 12-13 -16.333894807976602 2314 14-15 -16.25424775444038 2314 16-17 -18.654399482459276 2314 18-19 -15.753725623467478 2314 20-21 -16.253631986759537 2314 22-23 -15.767375985549382 2314 24-25 -18.15041176440218 2314 26-27 -17.079950376932086 2314 28-29 -16.123768410045543 2314 30-31 -17.746259167330287 2314 32-33 -15.368621312716137 2314 34-35 -16.201062306608236 2314 36-37 -16.50960119463165 2314 38-39 -15.518669776835274 2314 40-41 -17.673133126281726 2314 42-43 -17.0344355679073 2314 44-45 -14.619391459673857 2314 46-47 -13.0256405902485 2314 48-49 -12.752912623087362 2314 50-51 -12.6294343358286 2314 52-53 -11.969471660936682 2314 54-55 -12.1960656203384 2314 56-57 -11.20137350083833 2314 58-59 -10.613184678029185 2314 60-61 -11.681058429855428 2314 62-63 -14.32681883407525 2314 64-65 -13.388131117716103 2314 66-67 -14.003147483078626 2314 68-69 -16.463117010645544 2314 70-71 -16.14135063684603 2314 72-73 -14.553607804523544 2314 74-75 -12.760142693940818 2314 76-77 -13.16213247519168 2314 78-79 -12.655224845443382 2314 80-81 -13.027682207410278 2314 82-83 -13.594140293516821 2314 84-85 -12.880111957572991 2314 86-87 -13.64403750615931 2314 88-89 -13.975886433226266 2314 90-91 -13.777303173364368 2314 92-93 -13.765059008207853 2314 94-95 -13.516862060626107 2314 96-97 -12.787170855709746 2314 98-99 -12.540448096409747 2314 100-101 -12.113393406897789 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82343.0 3 14279.0 4 10007.0 5 8233.0 6 8427.0 7 9319.0 8 10829.0 9 12573.0 10 13702.0 11 14992.0 12 16107.0 13 17321.0 14 19154.0 15 20873.0 16 22847.0 17 23644.0 18 22345.0 19 20611.0 20 20571.0 21 22045.0 22 25824.0 23 31019.0 24 38199.0 25 46278.0 26 54851.0 27 62928.0 28 70827.0 29 78986.0 30 88416.0 31 100262.0 32 113773.0 33 135973.0 34 163051.0 35 196050.0 36 238862.0 37 280011.0 38 239941.0 39 55416.0 40 49.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.306659801163185 20.107654524655857 14.168191834670266 23.417493839510694 2 14.834990383512503 20.472562673400148 40.78188609490817 23.910560848179184 3 17.962635289667343 25.860806043125123 30.43126783019721 25.745290837010327 4 11.643402072159109 17.43786854116001 39.75642968984242 31.16229969683846 5 12.666360296028895 37.652937519104846 35.60227823305933 14.07842395180693 6 29.925417502557494 37.979114907863796 18.538051854016928 13.557415735561785 7 26.41287878945159 32.369622021265066 23.04337012319156 18.174129066091783 8 25.081437147131645 35.28012925305371 21.03218910940876 18.606244490405885 9 25.486025154845844 16.261917327246763 21.442158013555265 36.80989950435213 10-11 23.774928925787332 26.547337159535783 29.315156276053884 20.36257763862301 12-13 24.903225002000234 24.198103257837026 28.90332059763899 21.995351142523745 14-15 22.279546036918244 25.70823232736441 26.638061748581006 25.374159887136344 16-17 21.569826436522185 28.832745071128198 27.29276396999022 22.304664522359396 18-19 21.454452936778267 27.913662042037053 29.222023760073107 21.409861261111573 20-21 22.33779245605789 27.16710624000372 29.037396673968903 21.45770462996949 22-23 22.251790827822695 26.938376756211174 28.810229339723968 21.99960307624216 24-25 21.981415022427857 27.230537753693827 28.84765167656725 21.940395547311063 26-27 21.80925358880891 27.29067020800003 28.910356676211624 21.98971952697944 28-29 21.706787294993646 27.262144460836975 29.039056521275153 21.992011722894233 30-31 22.08251105507437 27.139293937068302 28.87562999803982 21.902565009817504 32-33 21.916960472364412 27.368055239830774 28.700597202508888 22.01438708529593 34-35 22.195324018699605 27.34492231447595 28.80388447350817 21.655869193316278 36-37 22.09988993416817 26.98843270408905 28.766701970801407 22.144975390941372 38-39 21.96887623820254 27.36373360545184 28.825103845895867 21.842286310449754 40-41 22.10729341517521 27.055778914816557 28.849760360253747 21.987167309754486 42-43 21.996390447079232 27.21045670000164 28.634886850086875 22.158266002832253 44-45 22.06736635144734 27.377460717976053 28.510286661527175 22.044886269049428 46-47 22.154864262316863 27.240927641165946 28.414221008099837 22.189987088417357 48-49 22.09256510592363 27.495552349418222 28.27651204550408 22.135370499154064 50-51 22.07455817714759 27.523139345854762 28.136992067696077 22.26531040930157 52-53 22.11899653421857 27.68571075089717 27.968499085810887 22.22679362907337 54-55 21.960965328678206 27.577924979343983 28.020001181532482 22.44110851044533 56-57 22.046128127213848 27.652492148481343 27.867081253205367 22.434298471099442 58-59 22.032822975666804 27.658944021574023 28.01553545938432 22.292697543374853 60-61 22.17452039969548 27.75407573275947 27.83957342550577 22.231830442039275 62-63 22.07756897937649 27.64253995747713 27.935620907713098 22.34427015543328 64-65 21.97478740639535 27.471797283878725 27.971001328113786 22.582413981612138 66-67 21.943368099884776 27.41408115845368 27.925272238439973 22.717278503221568 68-69 21.923666224515106 27.403379929305522 27.95517761136952 22.717776234809854 70-71 21.995940998897527 27.302485588596635 27.92832084441823 22.773252568087607 72-73 22.151295470891412 27.362586677882216 27.847418722505513 22.638699128720855 74-75 21.96462538646784 27.646604765448135 27.84358204151247 22.54518780657155 76-77 22.016493165730516 27.485526380188958 27.815895721914043 22.682084732166487 78-79 21.91634542240406 27.546871798445256 27.720725294470448 22.816057484680236 80-81 21.99641799166963 27.56373245599845 27.680491991083965 22.759357561247946 82-83 22.14781134977341 27.455496574362343 27.848434924498267 22.548257151365984 84-85 22.10753656875457 27.44664109985408 27.624269060423785 22.821553270967566 86-87 21.896996106909427 27.50412909830116 27.795302077448696 22.80357271734072 88-89 22.13918400224311 27.29180509826466 27.693889266335344 22.875121633156887 90-91 22.056996073727323 27.226954820074177 27.81137465998711 22.90467444621139 92-93 22.197543030969687 27.267602899784233 27.774272917843597 22.760581151402484 94-95 21.825364235828545 27.19651023792399 27.83091062482735 23.14721490142011 96-97 21.97080555368906 27.09090818594257 27.81919319368644 23.11909306668193 98-99 21.96715552204163 27.148416923205822 27.61151468847395 23.272912866278602 100-101 21.90085352671865 27.05604623594634 27.613422659562374 23.429677577772633 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 304.0 1 207.5 2 137.0 3 168.0 4 134.5 5 149.0 6 256.5 7 469.5 8 817.5 9 1041.5 10 1012.5 11 957.5 12 1070.0 13 1215.0 14 1364.5 15 1556.0 16 1765.5 17 1939.0 18 2139.5 19 2402.5 20 2727.5 21 3201.0 22 3901.5 23 4946.5 24 6352.0 25 8335.5 26 11166.0 27 14726.5 28 18704.5 29 23925.5 30 30398.5 31 36740.5 32 43555.0 33 51413.5 34 59051.5 35 65285.0 36 72579.0 37 79514.5 38 82120.0 39 83094.5 40 84402.5 41 84853.0 42 84577.5 43 85264.0 44 87146.5 45 89241.5 46 88358.0 47 86587.0 48 87465.5 49 87572.0 50 86402.5 51 83888.5 52 79382.5 53 74978.5 54 70814.0 55 65395.5 56 59411.5 57 53215.5 58 47167.0 59 41279.0 60 34855.0 61 28698.5 62 22734.5 63 17506.5 64 13685.0 65 10408.5 66 7792.0 67 6322.5 68 5240.0 69 4122.5 70 3098.5 71 2210.5 72 1569.0 73 1195.5 74 878.0 75 619.0 76 462.0 77 335.0 78 241.0 79 186.5 80 143.0 81 107.5 82 76.0 83 63.0 84 63.5 85 56.5 86 39.5 87 27.0 88 23.5 89 16.0 90 10.0 91 11.0 92 10.0 93 8.0 94 7.0 95 6.5 96 6.0 97 4.5 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034053136165260164 2 0.1930369009904029 3 0.0 4 0.3972727627172495 5 0.26724038527743144 6 0.09664288339227306 7 0.10680490331978673 8 0.10945947179064747 9 0.10680490331978673 10-11 0.12723263725570713 12-13 0.20616457163145632 14-15 0.1276266747631005 16-17 0.11848085682833819 18-19 0.1467478632797691 20-21 0.10543614145199919 22-23 0.10016848214263493 24-25 0.09647697286284425 26-27 0.14759815474309168 28-29 0.11028902443779144 30-31 0.12418403127745302 32-33 0.10197275915017308 34-35 0.14548279549287454 36-37 0.13637845519046943 38-39 0.1312144899619982 40-41 0.10931430007739727 42-43 0.1081114487390385 44-45 0.08872065561204809 46-47 0.09324171753898275 48-49 0.09801164526006062 50-51 0.0851950568616862 52-53 0.09315876227426836 54-55 0.30195716356040675 56-57 0.32105761326089677 58-59 0.3338119852107354 60-61 0.15991701155317972 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2410938.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.4402124557345 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39844970252938 55.41010716714939 2 10.21423076206055 13.572713238064038 3 2.9137561305752344 5.807717290788523 4 1.1496500936671836 3.0553198589201096 5 0.6016609301547374 1.9987240012897798 6 0.3559659321924822 1.4190271297123258 7 0.24439138059113505 1.1366190674177696 8 0.15864215442594126 0.8432174755595984 9 0.13613332435440412 0.8140254293170822 >10 0.7350300454061977 9.199256123880954 >50 0.06455793647823879 2.953032461834408 >100 0.02663255634559203 3.044941660333704 >500 7.063973863396052E-4 0.2950817807472777 >1k 1.2843588842494E-4 0.23513529405634986 >5k 6.421794421247E-5 0.2150820209286989 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5041 0.2090887447126388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3398 0.1409409947497613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 4.147763235719873E-5 8 0.0 0.0 0.0 0.0 4.147763235719873E-5 9 0.0 4.147763235719873E-5 0.0 4.147763235719873E-5 4.147763235719873E-5 10-11 0.0 4.147763235719873E-5 0.0 1.244328970715962E-4 4.147763235719873E-5 12-13 0.0 8.295526471439746E-5 0.0 1.4517171325019557E-4 4.147763235719873E-5 14-15 0.0 1.244328970715962E-4 0.0 1.6591052942879493E-4 4.147763235719873E-5 16-17 0.0 1.244328970715962E-4 0.0 1.6591052942879493E-4 8.295526471439746E-5 18-19 0.0 1.244328970715962E-4 0.0 1.6591052942879493E-4 8.295526471439746E-5 20-21 0.0 1.244328970715962E-4 0.0 1.8664934560739431E-4 1.0369408089299684E-4 22-23 0.0 1.244328970715962E-4 0.0 3.1108224267899047E-4 1.244328970715962E-4 24-25 0.0 1.4517171325019557E-4 0.0 6.22164485357981E-4 1.244328970715962E-4 26-27 0.0 1.6591052942879493E-4 0.0 0.0010784184412871672 1.244328970715962E-4 28-29 0.0 1.6591052942879493E-4 0.0 0.0024057026767175266 1.244328970715962E-4 30-31 0.0 2.0738816178599367E-4 0.0 0.00786001133168916 1.4517171325019557E-4 32-33 0.0 2.0738816178599367E-4 0.0 0.02065586091388497 1.8664934560739431E-4 34-35 0.0 2.0738816178599367E-4 0.0 0.04156058762191313 2.0738816178599367E-4 36-37 0.0 2.0738816178599367E-4 0.0 0.07617367182399548 2.0738816178599367E-4 38-39 0.0 2.0738816178599367E-4 0.0 0.12233827663755767 2.2812697796459303E-4 40-41 0.0 2.0738816178599367E-4 0.0 0.1700168150321576 2.488657941431924E-4 42-43 0.0 2.2812697796459303E-4 0.0 0.22144907915508405 2.488657941431924E-4 44-45 0.0 2.488657941431924E-4 0.0 0.28679708893385064 2.488657941431924E-4 46-47 0.0 2.488657941431924E-4 0.0 0.3661437996331718 2.488657941431924E-4 48-49 0.0 2.488657941431924E-4 0.0 0.44752291431799573 2.9034342650039114E-4 50-51 0.0 2.488657941431924E-4 0.0 0.5339415613342193 2.9034342650039114E-4 52-53 0.0 2.488657941431924E-4 0.0 0.621231238629944 2.9034342650039114E-4 54-55 0.0 2.488657941431924E-4 0.0 0.7166920095000369 2.9034342650039114E-4 56-57 0.0 2.488657941431924E-4 0.0 0.8299674234675467 3.1108224267899047E-4 58-59 0.0 2.488657941431924E-4 0.0 0.9555409554289658 3.3182105885758986E-4 60-61 0.0 2.488657941431924E-4 0.0 1.0866517513100709 3.3182105885758986E-4 62-63 0.0 2.488657941431924E-4 0.0 1.2144028589702431 3.3182105885758986E-4 64-65 0.0 2.488657941431924E-4 0.0 1.3546179951537534 3.3182105885758986E-4 66-67 0.0 2.488657941431924E-4 0.0 1.5143068797289687 3.3182105885758986E-4 68-69 0.0 2.488657941431924E-4 0.0 1.6889277119527752 3.5255987503618924E-4 70-71 0.0 2.488657941431924E-4 0.0 1.8791856115752459 3.732986912147886E-4 72-73 0.0 2.488657941431924E-4 0.0 2.0732387145584 4.977315882863848E-4 74-75 0.0 2.488657941431924E-4 0.0 2.272331349872954 4.977315882863848E-4 76-77 0.0 2.488657941431924E-4 0.0 2.488139471027459 4.977315882863848E-4 78-79 0.0 2.488657941431924E-4 0.0 2.730825097949429 4.977315882863848E-4 80-81 0.0 2.488657941431924E-4 0.0 2.9919475324541733 5.392092206435836E-4 82-83 0.0 2.488657941431924E-4 0.0 3.267587138283938 6.636421177151797E-4 84-85 0.0 2.488657941431924E-4 0.0 3.544636983613847 6.636421177151797E-4 86-87 0.0 2.488657941431924E-4 0.0 3.832118453481591 6.636421177151797E-4 88-89 0.0 2.488657941431924E-4 0.0 4.146270040955015 7.258585662509778E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2625 0.0 53.1245 1 GTATCAA 5375 0.0 42.470825 1 ATCAACG 6205 0.0 34.659584 3 CAACGCA 6315 0.0 34.359596 5 TATCAAC 6700 0.0 33.57279 2 TCAACGC 6495 0.0 33.4106 4 AACGCAG 6610 0.0 33.379818 6 ACGCAGA 7560 0.0 28.627504 7 CGCAGAG 7720 0.0 27.293694 8 GTACATG 6175 0.0 25.44487 1 GCAGAGT 8310 0.0 24.211138 9 TACATGG 6540 0.0 23.513615 2 ACATGGG 6230 0.0 23.34535 3 TAGTACT 1060 0.0 21.596699 4 GAGTACT 4665 0.0 20.845345 12-13 AGAGTAC 7365 0.0 20.730421 10-11 CAGAGTA 8230 0.0 19.765242 10-11 GTGGTAT 1485 0.0 18.974348 1 CATGGGG 4240 0.0 17.547318 4 GTACTTT 5625 0.0 17.448284 14-15 >>END_MODULE