FastQCFastQC Report
Sat 18 Jun 2016
SRR1931126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2410938
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62010.25720279824698933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48830.20253527880020142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32050.13293581170482194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34100.051.712991
GTATCAA61500.038.0250781
ATCAACG73950.031.015843
CAACGCA74750.030.61915
TCAACGC75000.030.5803494
TATCAAC78600.029.543432
AACGCAG79200.029.4982686
ACGCAGA88700.025.6429797
CGCAGAG92550.024.4223298
GTACATG69050.022.9912661
TACATGG72700.021.6859422
GCAGAGT103000.021.5296139
ACATGGG69900.021.1959083
GAGTACT60050.020.36201912-13
GTGGTAT16600.020.0433391
AGAGTAC92850.019.84295710-11
TCTAACG3254.9112714E-1018.9947952
CGTACGA1809.640644E-1118.46984160-61
CAGAGTA103200.018.45105710-11
GGCGTCG3357.348717E-1018.4270218
Produced by FastQC (version 0.11.5)