##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931126_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2410938 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.254692986713057 31.0 31.0 34.0 30.0 34.0 2 31.254388540891554 31.0 31.0 34.0 28.0 34.0 3 31.40868616281298 31.0 31.0 34.0 28.0 34.0 4 35.07344071062798 35.0 35.0 37.0 32.0 37.0 5 34.928596255897084 35.0 35.0 37.0 32.0 37.0 6 35.04911159059254 36.0 35.0 37.0 32.0 37.0 7 34.89902021536846 36.0 35.0 37.0 32.0 37.0 8 34.95082619295892 36.0 35.0 37.0 32.0 37.0 9 36.361860819315964 38.0 35.0 39.0 32.0 39.0 10-11 36.22062346688301 38.0 35.0 39.0 31.5 39.0 12-13 36.15944831430754 38.0 35.0 39.0 31.5 39.0 14-15 37.115271732412864 39.0 36.0 40.0 31.0 41.0 16-17 37.14810107103543 39.0 36.0 40.0 31.5 41.0 18-19 37.1801054195504 39.0 36.0 40.0 31.0 41.0 20-21 37.14686897796625 39.0 36.0 40.0 31.0 41.0 22-23 37.02395665089687 39.0 36.0 40.0 31.0 41.0 24-25 36.89992235387223 39.0 36.0 40.0 31.0 41.0 26-27 36.627942734321664 38.5 35.0 40.0 30.0 41.0 28-29 36.341040914366104 38.0 35.0 40.0 30.0 41.0 30-31 36.1259980555286 38.0 35.0 40.0 29.5 41.0 32-33 35.97752721140071 38.0 34.5 40.0 29.5 41.0 34-35 35.74329078557806 38.0 34.0 40.0 28.5 41.0 36-37 36.01275105373925 38.0 35.0 40.0 30.0 41.0 38-39 36.00208300669698 38.0 35.0 40.0 29.5 41.0 40-41 35.84696163899694 38.0 35.0 40.0 29.0 41.0 42-43 35.69887778117894 38.0 34.0 40.0 28.0 41.0 44-45 35.46153468069274 38.0 34.0 40.0 27.0 41.0 46-47 35.25244075127606 38.0 34.0 40.0 27.0 41.0 48-49 34.991416203983675 38.0 33.5 40.0 26.0 41.0 50-51 34.649350792098346 38.0 33.0 40.0 25.0 41.0 52-53 34.509907139876674 38.0 33.0 40.0 24.5 41.0 54-55 34.2687389721345 37.5 33.0 40.0 24.0 41.0 56-57 34.03889876056539 37.0 33.0 40.0 24.0 41.0 58-59 33.782012644041444 37.0 32.0 40.0 23.0 41.0 60-61 33.50197661656998 36.5 32.0 40.0 23.0 41.0 62-63 33.22230040755922 36.0 31.5 39.0 22.5 41.0 64-65 32.965833629898405 36.0 31.0 39.0 22.0 41.0 66-67 32.71352353316428 35.5 31.0 39.0 22.0 40.0 68-69 32.385134126219754 35.0 31.0 39.0 21.0 40.0 70-71 31.56279962404674 35.0 30.0 38.0 17.5 40.0 72-73 31.50841539682895 35.0 30.0 37.5 19.0 40.0 74-75 31.184256915773034 34.0 30.0 37.0 18.0 39.0 76-77 30.459583365478498 34.0 29.0 36.0 18.0 39.0 78-79 30.47970126150071 34.0 30.0 36.0 16.0 39.0 80-81 30.20162360044099 34.0 29.0 36.0 13.0 37.5 82-83 29.851153990687443 34.0 29.0 35.0 9.5 37.0 84-85 29.497620013455347 34.0 29.0 35.0 7.0 37.0 86-87 29.157388535084685 34.0 29.0 35.0 6.0 36.0 88-89 28.8402014070872 34.0 29.0 35.0 2.0 36.0 90-91 28.53973702351533 33.5 29.0 35.0 2.0 35.5 92-93 28.181332950080012 33.0 28.0 35.0 2.0 35.0 94-95 27.78916587651777 33.0 27.0 35.0 2.0 35.0 96-97 27.417238643216873 33.0 27.0 35.0 2.0 35.0 98-99 26.926295284242066 33.0 26.0 35.0 2.0 35.0 100-101 26.224337166696117 32.5 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7563577572466436 1101 2 0.6565864829099404 1101 3 0.5395162696108748 1101 4 0.7456645228818033 1101 5 0.7762114641139277 1101 6 0.8469042669138176 1101 7 0.7592436806377023 1101 8 0.7456931102659397 1101 9 1.0889314275319322 1101 10-11 1.0135971200706564 1101 12-13 0.9800071491138098 1101 14-15 1.372659770500448 1101 16-17 1.1254503398611675 1101 18-19 0.9930666600484557 1101 20-21 0.8993877217723067 1101 22-23 0.6941303653146136 1101 24-25 0.6994354315588112 1101 26-27 0.9488821414748116 1101 28-29 0.9233706090840599 1101 30-31 1.1674200382593511 1101 32-33 1.055908248330276 1101 34-35 0.987053642233235 1101 36-37 1.1509247279501338 1101 38-39 0.9865497014011524 1101 40-41 0.971070340807394 1101 42-43 1.0572053862811686 1101 44-45 0.9633511204546039 1101 46-47 1.143033424502697 1101 48-49 1.0385938853515526 1101 50-51 0.905300016715664 1101 52-53 0.782224664124449 1101 54-55 0.8161812108940367 1101 56-57 0.6010171186098887 1101 58-59 0.7341179357324918 1101 60-61 0.7090774839683931 1101 62-63 0.5649781767791495 1101 64-65 0.5630325147132922 1101 66-67 0.3207771525107006 1101 68-69 0.29945134125304307 1101 70-71 0.5401964389457738 1101 72-73 -0.010112755528258077 1101 74-75 -0.3466355268878125 1101 76-77 -0.13526255426540956 1101 78-79 -0.11137156454674724 1101 80-81 -0.12810771841557056 1101 82-83 -0.09275543895783755 1101 84-85 -0.12780006040919645 1101 86-87 -0.17222914870220052 1101 88-89 -0.18969210439566808 1101 90-91 -0.28987689482929113 1101 92-93 -0.5185064889377067 1101 94-95 -0.487143106517113 1101 96-97 -0.47690361488402644 1101 98-99 -0.4429680008436918 1101 100-101 -0.49829071883762666 1102 1 0.9417696249535332 1102 2 0.8864839421785398 1102 3 0.6894914654470057 1102 4 0.9215226304499851 1102 5 1.0391783444433855 1102 6 0.9869223515131935 1102 7 1.0338491010446873 1102 8 1.098127100618143 1102 9 1.444780922840046 1102 10-11 1.3736349145585294 1102 12-13 1.2959061203495779 1102 14-15 1.7983897132311597 1102 16-17 1.6366335070784501 1102 18-19 1.3750880015854463 1102 20-21 1.3247423832636258 1102 22-23 0.969881124569099 1102 24-25 0.7536757029810488 1102 26-27 1.0874177361666924 1102 28-29 1.416692797528043 1102 30-31 1.6717086800164864 1102 32-33 1.6967304548880477 1102 34-35 1.626780420602131 1102 36-37 1.819701985620334 1102 38-39 1.575642781208849 1102 40-41 1.5683994351142516 1102 42-43 1.7219644058930115 1102 44-45 1.767226470682779 1102 46-47 1.8636333229924986 1102 48-49 1.579983217731467 1102 50-51 1.4731618060484877 1102 52-53 1.4481354080205264 1102 54-55 1.2263080131574924 1102 56-57 1.2803229450107665 1102 58-59 1.4584161488683591 1102 60-61 1.7417698254423684 1102 62-63 1.5672034623893083 1102 64-65 1.6304558982034933 1102 66-67 1.3373031319337443 1102 68-69 1.2019527070849243 1102 70-71 1.529814891638214 1102 72-73 1.1642346880217431 1102 74-75 0.9989239804567127 1102 76-77 0.9953783991944505 1102 78-79 0.9600529857321582 1102 80-81 0.8230770250923811 1102 82-83 0.9785143491848416 1102 84-85 1.0425056883760462 1102 86-87 0.8988951557347811 1102 88-89 0.9049316497730437 1102 90-91 0.8940350468183453 1102 92-93 0.8232460470841048 1102 94-95 0.8148617798797133 1102 96-97 0.8150199700471568 1102 98-99 0.7676780182222345 1102 100-101 0.6883221582999504 1103 1 0.6247683001473376 1103 2 0.42590477684504435 1103 3 0.29194596129090655 1103 4 0.6378314860788663 1103 5 0.74339525295143 1103 6 0.6341189190977659 1103 7 0.6297057511513628 1103 8 0.6232322249651645 1103 9 0.9358934335110547 1103 10-11 0.8776782650047679 1103 12-13 0.9136333396346856 1103 14-15 1.2073936590650618 1103 16-17 0.9858143524610767 1103 18-19 0.836845372286966 1103 20-21 0.8836806364869929 1103 22-23 0.5628893213836221 1103 24-25 0.40144052155750387 1103 26-27 0.7593761510854762 1103 28-29 0.657004128548806 1103 30-31 1.1025281642966291 1103 32-33 1.1066250379131475 1103 34-35 0.8893657143248817 1103 36-37 1.0411685249008542 1103 38-39 0.9233847163898403 1103 40-41 0.9159063030017833 1103 42-43 1.0150681005885573 1103 44-45 1.1221146028541824 1103 46-47 1.218909382704446 1103 48-49 1.0283731541284595 1103 50-51 0.9282621273340652 1103 52-53 0.46032046401051474 1103 54-55 0.6452779463475764 1103 56-57 0.704273733243852 1103 58-59 0.823078286775278 1103 60-61 0.8786558767739905 1103 62-63 0.6814482795636536 1103 64-65 0.5761903018419616 1103 66-67 0.5034730180112632 1103 68-69 0.3530613598668815 1103 70-71 0.647005835134113 1103 72-73 0.2176422222558152 1103 74-75 0.03895711678176639 1103 76-77 0.1284234358240539 1103 78-79 0.147567597565196 1103 80-81 0.122137392292899 1103 82-83 0.30187104545473886 1103 84-85 0.3057626178406281 1103 86-87 0.2030527771051922 1103 88-89 -0.07032945409858371 1103 90-91 -0.14553499676704007 1103 92-93 -0.4924675325080159 1103 94-95 -0.5375773543048226 1103 96-97 -0.6978881793313931 1103 98-99 -0.7699558825031616 1103 100-101 -0.8475052217166201 1104 1 0.46069569432032154 1104 2 0.3707341335893517 1104 3 0.1944526977920198 1104 4 0.5718407827163006 1104 5 0.3596862247521315 1104 6 0.6038281523040112 1104 7 0.4768628022171768 1104 8 0.4930898327301563 1104 9 0.8044586424559839 1104 10-11 0.6374017569341603 1104 12-13 0.5504156211117177 1104 14-15 1.0350578447583914 1104 16-17 0.8464168247030202 1104 18-19 0.6255495052415867 1104 20-21 0.6416178776670236 1104 22-23 0.330480736910701 1104 24-25 0.3555625011070731 1104 26-27 0.1519472979325016 1104 28-29 0.5409778096612285 1104 30-31 0.9037149105037656 1104 32-33 0.9027245409433604 1104 34-35 0.6992193481251263 1104 36-37 0.9720184361461932 1104 38-39 0.5353151456249563 1104 40-41 0.7895928794994589 1104 42-43 0.9090767986807933 1104 44-45 0.9806989662883296 1104 46-47 1.0114159721761595 1104 48-49 0.9090672976347847 1104 50-51 0.8036563564198431 1104 52-53 0.5599323585426816 1104 54-55 0.8811275400719865 1104 56-57 0.7486276271609995 1104 58-59 0.6564472912475807 1104 60-61 0.9831421086180754 1104 62-63 0.04493088562111325 1104 64-65 -0.8865634763703412 1104 66-67 -0.2488110992998429 1104 68-69 0.2524468499841568 1104 70-71 0.8961858334218995 1104 72-73 0.49966965648127726 1104 74-75 0.14722291652418207 1104 76-77 -0.004491842188201645 1104 78-79 -0.08376561286129558 1104 80-81 0.00789018123720453 1104 82-83 -0.15433382447169208 1104 84-85 -0.2556118577936317 1104 86-87 -0.0013543716934591998 1104 88-89 -0.08318143960544688 1104 90-91 0.06828977589266927 1104 92-93 0.14152340631013516 1104 94-95 0.046562198907253105 1104 96-97 0.22684072088142315 1104 98-99 0.1344353068280526 1104 100-101 -0.1443494129970695 1105 1 -0.25526247080813036 1105 2 -1.743905926370509 1105 3 -0.35219122459697516 1105 4 0.3692540693572752 1105 5 0.6101242217829537 1105 6 0.08253910499917794 1105 7 -0.1660218150760926 1105 8 -0.2779055143618052 1105 9 0.5261185008869447 1105 10-11 0.9312454021913013 1105 12-13 0.9753213095183213 1105 14-15 1.2612486296760892 1105 16-17 0.8285739324658792 1105 18-19 0.6916992070270922 1105 20-21 1.0145506504614943 1105 22-23 0.30133188323317484 1105 24-25 -0.162802030370095 1105 26-27 0.8566282841453798 1105 28-29 0.4879061956019086 1105 30-31 0.8819734108733996 1105 32-33 0.8412918115307875 1105 34-35 0.479602024438897 1105 36-37 0.6484677035044726 1105 38-39 0.7576649202152126 1105 40-41 0.8411783676001718 1105 42-43 1.2161635749515725 1105 44-45 1.2293942918974494 1105 46-47 1.3664641676975435 1105 48-49 1.2057104356279424 1105 50-51 1.0530352269730727 1105 52-53 0.9438221807025329 1105 54-55 0.10434684146024154 1105 56-57 0.32794266069952016 1105 58-59 -0.4528181524659445 1105 60-61 0.2670446732082681 1105 62-63 0.7784703069547021 1105 64-65 1.19114085634412 1105 66-67 1.0579286858653418 1105 68-69 0.8231674024037297 1105 70-71 0.34600764750215873 1105 72-73 -0.33689365883016364 1105 74-75 -0.28123931865817653 1105 76-77 -0.07263070778533631 1105 78-79 -0.5582442537626413 1105 80-81 -0.34305335090685674 1105 82-83 -0.20076912958406368 1105 84-85 -0.15594748316361517 1105 86-87 -0.534072290463989 1105 88-89 -0.7710528550365296 1105 90-91 -1.122541149025281 1105 92-93 -0.589817113369719 1105 94-95 -0.7861101608723899 1105 96-97 -0.9739357029330904 1105 98-99 -1.6238475450303085 1105 100-101 -0.9801287806971857 1106 1 0.36723324158903026 1106 2 0.38308307925286655 1106 3 -0.2502067383629445 1106 4 0.4028650811313099 1106 5 0.18070333722837972 1106 6 0.4500123886961944 1106 7 0.40117597380595527 1106 8 0.47062234601788333 1106 9 0.82736291698005 1106 10-11 0.29050750135726133 1106 12-13 0.2625860104206552 1106 14-15 0.010581997374686125 1106 16-17 -0.1959051564538683 1106 18-19 0.4302989802879864 1106 20-21 0.553842704676974 1106 22-23 0.4705539647726269 1106 24-25 0.3799188532179514 1106 26-27 0.6541007910443781 1106 28-29 0.7146522102164496 1106 30-31 0.27670267528863235 1106 32-33 0.26766539266626665 1106 34-35 -0.9339964153784024 1106 36-37 -0.7244936402343711 1106 38-39 -0.797276785926762 1106 40-41 0.5235159854935958 1106 42-43 0.8013802138933457 1106 44-45 0.7101484589534053 1106 46-47 0.30488552799614865 1106 48-49 0.29774095340855666 1106 50-51 0.7996440382967123 1106 52-53 0.20897917444412428 1106 54-55 0.8843064909202951 1106 56-57 0.5429520449206393 1106 58-59 0.8835531412320776 1106 60-61 1.0275958275050598 1106 62-63 0.8346588633955179 1106 64-65 0.9163993922624343 1106 66-67 0.550272822418556 1106 68-69 0.4984409194265247 1106 70-71 0.8379076846953843 1106 72-73 0.3345824614324897 1106 74-75 0.40100103839684564 1106 76-77 0.5417828687824269 1106 78-79 0.4712184060581812 1106 80-81 0.4624893355756754 1106 82-83 0.41337518087167524 1106 84-85 0.6892651321792549 1106 86-87 0.5435671313548696 1106 88-89 0.4697993460761154 1106 90-91 0.6442929408806748 1106 92-93 0.06759488203822173 1106 94-95 0.3401490483901952 1106 96-97 0.7276702643075126 1106 98-99 0.7380110283318189 1106 100-101 0.635243801340021 1107 1 0.46453948891901575 1107 2 0.4457233074189091 1107 3 0.18700793056691012 1107 4 -0.9920098555150787 1107 5 0.20718372982071998 1107 6 0.39838789333051494 1107 7 0.561427141416651 1107 8 0.4886494511444326 1107 9 0.816507588844587 1107 10-11 0.7824533068376311 1107 12-13 0.8223494595891196 1107 14-15 1.1673623994550724 1107 16-17 1.0275564817440141 1107 18-19 0.759728765688223 1107 20-21 0.15264032205372047 1107 22-23 0.39896129515069134 1107 24-25 0.5288042662460555 1107 26-27 0.9101847759337218 1107 28-29 0.719125265062857 1107 30-31 1.022922780612717 1107 32-33 1.0655863102884027 1107 34-35 0.9116137109143665 1107 36-37 1.1843309859493658 1107 38-39 1.0281246550482095 1107 40-41 0.32782867404942095 1107 42-43 0.060546999920141786 1107 44-45 -0.12511465506332797 1107 46-47 0.5708419307373447 1107 48-49 0.7836886605284406 1107 50-51 -0.838773531309748 1107 52-53 0.5688443539395323 1107 54-55 1.0485183682141752 1107 56-57 0.8788584564735373 1107 58-59 0.9379666343343374 1107 60-61 1.1208522407895956 1107 62-63 1.232277132335625 1107 64-65 1.3582175887326287 1107 66-67 1.1149290935454204 1107 68-69 0.882786134057465 1107 70-71 1.4437619081300674 1107 72-73 1.0137656006765 1107 74-75 0.8688035434150088 1107 76-77 0.9224964815070464 1107 78-79 0.8502697391005718 1107 80-81 0.9763505243334976 1107 82-83 1.0569769774422966 1107 84-85 0.9775982255131836 1107 86-87 0.9182601008049787 1107 88-89 0.9955900082029743 1107 90-91 1.196940975343118 1107 92-93 1.1113238607046938 1107 94-95 1.092363270738879 1107 96-97 1.0420394715237649 1107 98-99 1.1349286301247439 1107 100-101 0.825856588412325 1108 1 0.5561810603833202 1108 2 0.3937756340390237 1108 3 0.24916903115396138 1108 4 0.5349378111482679 1108 5 0.4973176279158835 1108 6 0.48380673223557125 1108 7 0.42242448193864135 1108 8 0.40924929655673026 1108 9 0.7149533564756823 1108 10-11 0.7195070436608049 1108 12-13 0.798939726152156 1108 14-15 1.074010463119194 1108 16-17 0.6630392083013703 1108 18-19 0.6465320296003796 1108 20-21 0.5080167312828792 1108 22-23 0.538333766880136 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1.6207300527499626 2311 82-83 2.21361202372114 2311 84-85 1.9300933044594473 2311 86-87 1.5896970484952249 2311 88-89 2.017308607692023 2311 90-91 2.1584363474585437 2311 92-93 2.0639100314966683 2311 94-95 2.0281655072007467 2311 96-97 2.218188066405869 2311 98-99 2.1306179964605505 2311 100-101 2.400215804394236 2312 1 -0.16422021157862332 2312 2 -0.19124678178397048 2312 3 -0.3569867316136417 2312 4 0.28359451223843735 2312 5 -1.1410773086877484 2312 6 0.09341258785737949 2312 7 0.2172173113204252 2312 8 -2.640674353939815 2312 9 -5.922764887571319 2312 10-11 -1.005634743907855 2312 12-13 -5.750733603114092 2312 14-15 -1.8905053563267558 2312 16-17 -2.3980657627909565 2312 18-19 -2.328414758035528 2312 20-21 -3.5120077445707594 2312 22-23 -2.3375643636879033 2312 24-25 -2.033447341856103 2312 26-27 -2.877087666862188 2312 28-29 -5.934331225178621 2312 30-31 -6.763484270791594 2312 32-33 -1.7013247047183953 2312 34-35 0.5429155996464345 2312 36-37 1.0826140390838148 2312 38-39 0.754567550530787 2312 40-41 -2.463561893863279 2312 42-43 -5.026659689940001 2312 44-45 -2.83915048233159 2312 46-47 0.5304006425487557 2312 48-49 -1.219768379270512 2312 50-51 -7.73578128782863 2312 52-53 -1.7758833532614169 2312 54-55 0.7663868739803306 2312 56-57 1.3819689517929206 2312 58-59 1.2845922544453003 2312 60-61 1.9408611759476244 2312 62-63 1.6862261553840625 2312 64-65 1.9703842093873334 2312 66-67 1.594341054114345 2312 68-69 1.638924206171044 2312 70-71 2.3416331164259283 2312 72-73 1.9449988270508598 2312 74-75 1.5261305346395453 2312 76-77 1.4287636996277016 2312 78-79 1.6576126674919394 2312 80-81 1.6017954234379772 2312 82-83 0.816866264239529 2312 84-85 0.9657374820847586 2312 86-87 1.2855422933970715 2312 88-89 1.4369150494682756 2312 90-91 1.53305517726605 2312 92-93 1.9460522079784681 2312 94-95 1.8743347467662161 2312 96-97 1.9817863130229831 2312 98-99 2.1112116690353524 2312 100-101 2.385593162884799 2313 1 -0.6066842282838216 2313 2 -0.37101410224038034 2313 3 -0.13055299307982082 2313 4 -1.2633515687519434 2313 5 -2.421784296531733 2313 6 -2.359920994065213 2313 7 -1.2561214909765397 2313 8 -4.09449268491155 2313 9 -3.28397750596195 2313 10-11 -1.1972784160683503 2313 12-13 -2.206584151253402 2313 14-15 -1.936146343723287 2313 16-17 -4.416700634891786 2313 18-19 -4.19817573946591 2313 20-21 -5.152799377056397 2313 22-23 -2.2176355845097433 2313 24-25 -0.028059169732905787 2313 26-27 -4.271225932206363 2313 28-29 -4.547799791067202 2313 30-31 -4.3189973590159205 2313 32-33 -5.98943343765896 2313 34-35 -0.6089413620956776 2313 36-37 -3.785692695199124 2313 38-39 -1.2703131266263554 2313 40-41 -0.5801999727655058 2313 42-43 -2.0628387487810826 2313 44-45 -3.171958670861681 2313 46-47 0.00626573315953749 2313 48-49 0.18490385579548985 2313 50-51 1.5783547402122338 2313 52-53 1.1731807081898467 2313 54-55 -3.0006445974194733 2313 56-57 -3.2297470598410527 2313 58-59 0.4936029935011632 2313 60-61 -5.654662353025451 2313 62-63 -0.7892940176340559 2313 64-65 -0.850426440176399 2313 66-67 -2.69847533786119 2313 68-69 -4.664836327798678 2313 70-71 -4.991846263095287 2313 72-73 0.14822986590536402 2313 74-75 2.194058791780275 2313 76-77 0.7206105935049472 2313 78-79 1.7885881317323786 2313 80-81 2.4119136276291897 2313 82-83 2.416631347392638 2313 84-85 2.374054657116453 2313 86-87 2.2399385943889456 2313 88-89 -1.9800343874770618 2313 90-91 1.622808328134873 2313 92-93 2.0988134131391796 2313 94-95 2.5926582608239386 2313 96-97 2.6184296122995896 2313 98-99 2.975062440904992 2313 100-101 3.399128847872497 2314 1 -28.737119010892517 2314 2 -15.006357720753703 2314 3 -4.8889681403449 2314 4 -2.8482043036748053 2314 5 -0.9652625574012959 2314 6 -0.6407765340371654 2314 7 0.22634695222121337 2314 8 -4.204941492765688 2314 9 -3.539236973002623 2314 10-11 -0.4237861299533421 2314 12-13 0.7857019637536098 2314 14-15 -0.44070454849186547 2314 16-17 -0.11463191118912164 2314 18-19 -2.397509540307418 2314 20-21 -2.2308148048263874 2314 22-23 -6.6908123026023105 2314 24-25 -1.7987464067595482 2314 26-27 -3.5282385549272632 2314 28-29 -2.3090199874206405 2314 30-31 -3.823380248216484 2314 32-33 -2.603388556873547 2314 34-35 -3.2288572106230333 2314 36-37 -6.633343466587625 2314 38-39 -7.979815230413166 2314 40-41 -7.112458037281634 2314 42-43 -4.809858384124695 2314 44-45 -5.703340157537699 2314 46-47 -9.338229358004554 2314 48-49 -7.1916739843167115 2314 50-51 -2.8616873355240884 2314 52-53 -2.9253466410387645 2314 54-55 -5.14808499012635 2314 56-57 -1.8787652926601268 2314 58-59 -2.646824776484813 2314 60-61 -0.8582037274995002 2314 62-63 1.5245208491261053 2314 64-65 -1.8977616435424594 2314 66-67 -0.6190573855470234 2314 68-69 1.0800795207286704 2314 70-71 1.336169164674697 2314 72-73 0.8584332319642698 2314 74-75 0.39882737523048206 2314 76-77 -1.2034777655413365 2314 78-79 -2.416531082853883 2314 80-81 -2.4445029221604315 2314 82-83 -2.3386281196480354 2314 84-85 -0.18397479589897614 2314 86-87 0.8153242886385925 2314 88-89 0.7645308299142428 2314 90-91 1.2655152005051384 2314 92-93 1.1694655870522226 2314 94-95 1.188380560964191 2314 96-97 1.3792991775169803 2314 98-99 1.9307076021953158 2314 100-101 2.854248062458751 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 15.0 4 53.0 5 109.0 6 245.0 7 705.0 8 1743.0 9 2922.0 10 4331.0 11 6032.0 12 7852.0 13 9693.0 14 12310.0 15 14489.0 16 17031.0 17 18761.0 18 17570.0 19 15410.0 20 15216.0 21 16288.0 22 17982.0 23 20493.0 24 24528.0 25 30128.0 26 38035.0 27 47724.0 28 59659.0 29 74218.0 30 90557.0 31 110060.0 32 132486.0 33 159023.0 34 194386.0 35 237620.0 36 295164.0 37 357916.0 38 299733.0 39 60398.0 40 45.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.181326335501474 21.388369287877875 15.149281774149683 23.281022602470973 2 15.0368445808229 21.714204181111253 39.87970657063765 23.369244667428195 3 18.119331603649343 26.55060777445253 30.812184109720864 24.517876512177267 4 12.813032989786505 17.98714022152466 38.65814325438171 30.541683534307122 5 12.318706228081638 38.61381580175326 35.92398799635826 13.143489973806844 6 30.042373549216112 38.973918035221146 17.820906219902792 13.162802195659948 7 26.251359429400505 33.067959441511974 22.910087277234005 17.770593851853512 8 25.081150987706856 36.22507090601251 20.4042575960062 18.289520510274425 9 25.38273485257605 16.343307044809947 21.01937088386346 37.254587218750544 10-11 23.909988560468996 27.044888752842255 29.040356906730906 20.004765779957843 12-13 24.716500382838547 24.582797234935118 28.965738646120307 21.73496373610603 14-15 22.088083559179044 26.187069099246852 26.510781280978605 25.214066060595503 16-17 21.523884065040246 29.306892172258266 27.16845061963435 22.000773143067136 18-19 21.438191276590274 28.22994618691978 29.14956751272741 21.182295023762535 20-21 22.200404987602333 27.422915894145767 29.07022080202809 21.30645831622381 22-23 22.18271477740199 27.1875925469672 28.817808670318357 21.811884005312454 24-25 21.969580685896915 27.512416469889335 28.90527639817001 21.61272644604374 26-27 21.835070097635175 27.554528564003373 28.88422036836466 21.726180969996793 28-29 21.737963589001208 27.455730646546183 29.02495426848238 21.781351495970235 30-31 22.153411778759757 27.344460480473913 28.84459082650166 21.657536914264668 32-33 22.031842682135206 27.507771945506164 28.695661190875665 21.764724181482965 34-35 22.12706343009251 27.45594196316079 28.83977132854878 21.57722327819792 36-37 22.14145296318057 27.212412551336847 28.80077248695464 21.845361998527945 38-39 21.89236950389778 27.493291473872368 28.907085228094036 21.707253794135816 40-41 22.1189567563274 27.205264292005694 28.916005723210713 21.759773228456194 42-43 22.023143528910126 27.347374634033645 28.613057758445304 22.01642407861093 44-45 22.05676244594917 27.437995789997615 28.59099723135311 21.914244532700106 46-47 22.151496694435018 27.371526086455027 28.389272493566864 22.08770472554309 48-49 22.110178461752906 27.551066337857467 28.271824731338697 22.066930469050927 50-51 22.206511267985405 27.54791973658182 28.072248617409507 22.173320378023273 52-53 22.226971455449185 27.663599159738283 27.9626764189786 22.146752965833933 54-55 22.169708273384654 27.538689590142933 28.076334595065035 22.21526754140738 56-57 22.246878712947776 27.685542915532324 27.920765837054102 22.1468125344658 58-59 22.11793233088944 27.729759907885327 28.05167181825271 22.100635942972524 60-61 22.264210736113423 27.785056232370025 27.84822911639469 22.10250391512186 62-63 22.18083527749428 27.665204596799096 27.922914372784884 22.231045752921737 64-65 22.063021143800327 27.540369264644088 27.919929746051224 22.476679845504364 66-67 22.042712140003896 27.46748990994653 27.94652375362433 22.54327419642524 68-69 22.084468986960324 27.51683726185494 27.917231641816052 22.48146210936868 70-71 22.168459402076785 27.246163038782445 27.91610815438645 22.669269404754324 72-73 22.260438968554354 27.353580880786772 27.886804043702313 22.49917610695656 74-75 22.104120107444995 27.63757472288449 27.734156591624398 22.524148578046113 76-77 22.04380618663773 27.548157605048324 27.669002686921022 22.73903352139292 78-79 21.98592415068326 27.639470612682697 27.53314270213502 22.841462534499023 80-81 22.024790423284614 27.617360264561697 27.546205179658813 22.811644132494873 82-83 22.194021929725416 27.50226728001266 27.70059336060775 22.603117429654176 84-85 22.106508797202086 27.509097085489998 27.490162538465334 22.894231578842582 86-87 21.954180153166963 27.579800642789397 27.632229229061284 22.83378997498236 88-89 22.156018843248216 27.37908037747886 27.526829893518308 22.938070885754616 90-91 22.051530069853495 27.289961479163615 27.625313005127122 23.033195445855764 92-93 22.189313217107696 27.36125107404775 27.55815205094123 22.891283657903323 94-95 21.921239528104962 27.2584192532511 27.631140426829205 23.189200791814734 96-97 21.976041980558918 27.21593844355622 27.563045809586402 23.24497376629846 98-99 21.90102706820278 27.251495971510952 27.456461644997297 23.39101531528897 100-101 21.907332219069588 27.199822304037212 27.41173842772671 23.481107049166493 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 357.0 1 247.5 2 137.5 3 160.0 4 150.0 5 148.0 6 279.5 7 547.5 8 916.0 9 1211.5 10 1192.5 11 1044.0 12 1101.0 13 1272.5 14 1446.5 15 1665.0 16 1872.0 17 2031.0 18 2226.5 19 2445.0 20 2776.0 21 3273.0 22 3996.5 23 5052.5 24 6394.5 25 8429.0 26 11396.0 27 14986.5 28 19074.0 29 24617.0 30 31195.5 31 37238.0 32 44556.0 33 52490.0 34 59554.5 35 66142.5 36 73364.0 37 79966.5 38 82379.0 39 82784.0 40 83554.0 41 83550.0 42 83134.5 43 83953.5 44 86060.5 45 88617.5 46 88031.0 47 86364.5 48 86958.5 49 87457.5 50 87114.5 51 84567.0 52 79258.0 53 74708.5 54 71183.0 55 65664.5 56 59665.5 57 53618.5 58 47651.5 59 41796.5 60 34921.5 61 28361.5 62 22134.5 63 16860.5 64 13177.5 65 9756.0 66 7256.5 67 5978.0 68 5025.0 69 4030.5 70 2988.5 71 2115.0 72 1464.5 73 1106.5 74 838.5 75 586.5 76 410.0 77 282.5 78 205.5 79 150.5 80 103.0 81 79.0 82 59.5 83 46.5 84 37.5 85 33.5 86 26.0 87 17.5 88 17.0 89 11.0 90 3.0 91 1.5 92 2.5 93 2.0 94 0.0 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10672194805507235 2 0.0 3 0.003857419809219482 4 4.147763235719873E-4 5 1.2443289707159618E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.017835381913595457 26-27 0.00949837780979851 28-29 0.005309136941721438 30-31 0.0019701875369669396 32-33 9.539855442155708E-4 34-35 0.0023020085958245294 36-37 9.747243603941703E-4 38-39 5.599480368221829E-4 40-41 0.0022190533311101323 42-43 0.0010991572574657665 44-45 5.392092206435835E-4 46-47 0.031523000591471034 48-49 0.017482822038559265 50-51 0.08894878259001268 52-53 9.332467280369715E-4 54-55 0.03693583161408547 56-57 0.0013895006839661575 58-59 0.001244328970715962 60-61 0.0038781586253980815 62-63 0.0035878151988976903 64-65 0.010576796251085678 66-67 0.006138689588865412 68-69 0.0026960461032179174 70-71 0.005952040243258018 72-73 0.005288398125542839 74-75 0.001244328970715962 76-77 0.0 78-79 0.0 80-81 2.9034342650039114E-4 82-83 3.940375073933879E-4 84-85 4.147763235719873E-5 86-87 0.0016383664781093501 88-89 0.004189240868077072 90-91 0.0014517171325019557 92-93 0.0033804270371116966 94-95 8.502914633225741E-4 96-97 5.184704044649841E-4 98-99 0.001928709904609741 100-101 0.0032559941400401005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2410938.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.26316804750949 #Duplication Level Percentage of deduplicated Percentage of total 1 79.80555763110874 48.0933573064871 2 12.066122852231556 14.542855782518496 3 3.5941627711405264 6.4978690520203175 4 1.5480651570091397 3.731652428213438 5 0.7969367344039309 2.401296617430876 6 0.45218756066838905 1.6350152974531513 7 0.32037358126230575 1.3514708875914938 8 0.2089458047692505 1.0073388916505162 9 0.1665482843243699 0.9033054521637505 >10 0.9054529069319208 10.37028001066663 >50 0.09063902056360419 3.7589250229275937 >100 0.04361876099961513 4.596540802022084 >500 0.0010417009400295583 0.4208516367836236 >1k 2.7778691734099616E-4 0.42972400621435247 >5k 6.944672933524904E-5 0.259516805856564 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6201 0.25720279824698933 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4883 0.20253527880020142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3205 0.13293581170482194 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 4.147763235719873E-5 0.0 0.0 0.0 5 0.0 4.147763235719873E-5 0.0 0.0 0.0 6 0.0 4.147763235719873E-5 0.0 0.0 4.147763235719873E-5 7 0.0 4.147763235719873E-5 0.0 0.0 4.147763235719873E-5 8 0.0 4.147763235719873E-5 0.0 0.0 4.147763235719873E-5 9 0.0 8.295526471439746E-5 0.0 4.147763235719873E-5 4.147763235719873E-5 10-11 0.0 8.295526471439746E-5 0.0 1.244328970715962E-4 4.147763235719873E-5 12-13 0.0 8.295526471439746E-5 0.0 1.244328970715962E-4 4.147763235719873E-5 14-15 0.0 8.295526471439746E-5 0.0 1.244328970715962E-4 8.295526471439746E-5 16-17 0.0 8.295526471439746E-5 0.0 1.244328970715962E-4 8.295526471439746E-5 18-19 0.0 1.244328970715962E-4 0.0 1.244328970715962E-4 2.488657941431924E-4 20-21 0.0 1.4517171325019557E-4 0.0 1.4517171325019557E-4 4.1477632357198735E-4 22-23 0.0 1.6591052942879493E-4 0.0 2.696046103217918E-4 4.1477632357198735E-4 24-25 0.0 1.6591052942879493E-4 0.0 6.22164485357981E-4 4.977315882863848E-4 26-27 0.0 1.6591052942879493E-4 0.0 0.001140634889822965 4.977315882863848E-4 28-29 0.0 1.6591052942879493E-4 0.0 0.0024057026767175266 4.977315882863848E-4 30-31 0.0 1.6591052942879493E-4 0.0 0.008337004103796945 4.977315882863848E-4 32-33 0.0 1.6591052942879493E-4 0.0 0.022273488575815723 4.977315882863848E-4 34-35 0.0 2.488657941431924E-4 0.0 0.04502396992373922 4.977315882863848E-4 36-37 0.0 3.3182105885758986E-4 0.0 0.08177315219221731 4.977315882863848E-4 38-39 0.0 3.3182105885758986E-4 0.0 0.1310900570649266 5.184704044649841E-4 40-41 0.0 3.3182105885758986E-4 0.0 0.18183794025395925 5.392092206435835E-4 42-43 0.0 3.3182105885758986E-4 0.0 0.2363188103551398 5.599480368221829E-4 44-45 0.0 3.3182105885758986E-4 0.0 0.30525463533280406 5.806868530007823E-4 46-47 0.0 3.3182105885758986E-4 0.0 0.38883206453255953 5.806868530007823E-4 48-49 0.0 3.3182105885758986E-4 0.0 0.4742967260045675 5.806868530007823E-4 50-51 0.0 3.3182105885758986E-4 0.0 0.5656926889036549 5.806868530007823E-4 52-53 0.0 3.3182105885758986E-4 0.0 0.6572960399645282 5.806868530007823E-4 54-55 0.0 3.3182105885758986E-4 0.0 0.7560957602393757 5.806868530007823E-4 56-57 0.0 3.3182105885758986E-4 0.0 0.8712169288467808 5.806868530007823E-4 58-59 0.0 3.3182105885758986E-4 0.0 0.9973504088450222 5.806868530007823E-4 60-61 0.0 3.3182105885758986E-4 0.0 1.125889591519981 5.806868530007823E-4 62-63 0.0 3.3182105885758986E-4 0.0 1.2524793254741518 6.014256691793817E-4 64-65 0.0 3.3182105885758986E-4 0.0 1.391595304400196 6.429033015365804E-4 66-67 0.0 3.3182105885758986E-4 0.0 1.5495213896002302 6.843809338937791E-4 68-69 0.0 3.3182105885758986E-4 0.0 1.7233748856254287 7.258585662509778E-4 70-71 0.0 3.3182105885758986E-4 0.0 1.9117455529756469 8.295526471439747E-4 72-73 0.0 3.732986912147886E-4 0.0 2.1093449935253417 8.710302795011734E-4 74-75 0.0 3.732986912147886E-4 0.0 2.31073963743572 8.710302795011734E-4 76-77 0.0 3.732986912147886E-4 0.0 2.529409715222872 8.710302795011734E-4 78-79 0.0 3.732986912147886E-4 0.0 2.775600202079025 8.710302795011734E-4 80-81 0.0 3.732986912147886E-4 0.0 3.040497101128275 8.917690956797727E-4 82-83 0.0 3.732986912147886E-4 0.0 3.319289007017186 9.125079118583721E-4 84-85 0.0 3.732986912147886E-4 0.0 3.598205345803169 9.125079118583721E-4 86-87 0.0 3.732986912147886E-4 0.0 3.888092518347631 9.539855442155709E-4 88-89 0.0 3.732986912147886E-4 0.0 4.206412607872952 9.954631765727697E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3410 0.0 51.71299 1 GTATCAA 6150 0.0 38.025078 1 ATCAACG 7395 0.0 31.01584 3 CAACGCA 7475 0.0 30.6191 5 TCAACGC 7500 0.0 30.580349 4 TATCAAC 7860 0.0 29.54343 2 AACGCAG 7920 0.0 29.498268 6 ACGCAGA 8870 0.0 25.642979 7 CGCAGAG 9255 0.0 24.422329 8 GTACATG 6905 0.0 22.991266 1 TACATGG 7270 0.0 21.685942 2 GCAGAGT 10300 0.0 21.529613 9 ACATGGG 6990 0.0 21.195908 3 GAGTACT 6005 0.0 20.362019 12-13 GTGGTAT 1660 0.0 20.043339 1 AGAGTAC 9285 0.0 19.842957 10-11 TCTAACG 325 4.9112714E-10 18.994795 2 CGTACGA 180 9.640644E-11 18.469841 60-61 CAGAGTA 10320 0.0 18.451057 10-11 GGCGTCG 335 7.348717E-10 18.427021 8 >>END_MODULE