FastQCFastQC Report
Sat 18 Jun 2016
SRR1931125_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931125_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843819
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59880.2105619239480431No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45700.16069939753549717No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35050.050.19431
GTATCAA66400.044.9273831
ATCAACG81650.035.1421553
TCAACGC82900.034.846564
CAACGCA83000.034.3767475
AACGCAG84200.034.146336
TATCAAC86750.034.0024572
ACGCAGA93800.029.6482627
CGCAGAG93650.029.2373438
GCAGAGT103450.024.9057549
GTACATG69600.022.1177651
GAGTACT63950.021.74053812-13
GTGGTAT17500.021.5816021
AGAGTAC91850.021.47791710-11
TACATGG69400.021.217092
CAGAGTA103050.020.61971910-11
ACATGGG69600.020.3392683
CCGTCGA3302.7284841E-1120.167179
TGGTATC19850.019.02662
GTACTTT74250.018.97472414-15
Produced by FastQC (version 0.11.5)