##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931125_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2843819 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.497693418603646 31.0 28.0 31.0 22.0 34.0 2 28.19166655824439 31.0 28.0 33.0 16.0 34.0 3 28.23251092984469 31.0 28.0 33.0 16.0 34.0 4 31.406247373690096 35.0 31.0 37.0 17.0 37.0 5 31.468176420510588 35.0 32.0 37.0 17.0 37.0 6 31.734945859775184 35.0 32.0 37.0 19.0 37.0 7 31.399827485504527 35.0 31.0 37.0 17.0 37.0 8 31.545195386907537 35.0 32.0 37.0 17.0 37.0 9 32.523219304744785 37.0 31.0 39.0 17.0 39.0 10-11 32.52829751119885 37.0 31.5 39.0 17.0 39.0 12-13 32.603346767146576 37.0 32.0 39.0 17.0 39.0 14-15 33.25907292271414 38.0 31.0 40.0 17.0 41.0 16-17 33.243473477039146 37.5 31.0 40.0 17.0 41.0 18-19 33.3201759675985 37.5 31.5 40.0 17.5 41.0 20-21 33.53567034329541 38.0 32.0 40.0 18.0 41.0 22-23 33.42634271027798 38.0 32.0 40.0 18.0 41.0 24-25 33.30148314643091 37.5 31.5 40.0 17.5 41.0 26-27 32.92533737203387 37.0 31.0 40.0 16.5 41.0 28-29 32.8864069759714 37.0 31.0 40.0 16.5 41.0 30-31 32.63284038119163 37.0 30.5 40.0 15.5 41.0 32-33 32.4321827444011 37.0 30.0 40.0 15.5 41.0 34-35 32.4486257740032 37.0 30.0 40.0 15.5 41.0 36-37 32.070784040756465 36.0 30.0 40.0 9.5 41.0 38-39 31.87413703192784 36.0 30.0 40.0 9.0 41.0 40-41 31.734259810487238 36.0 30.0 40.0 9.0 41.0 42-43 31.961686380181018 37.0 30.0 40.0 9.0 41.0 44-45 32.03133480014023 37.0 30.0 40.0 8.5 41.0 46-47 31.785228068312364 36.5 30.0 40.0 7.5 41.0 48-49 31.560370051680504 36.0 30.0 40.0 4.5 41.0 50-51 31.04385018877784 35.5 29.0 39.5 2.0 40.5 52-53 30.982550049774616 36.0 29.0 39.5 2.0 40.5 54-55 30.98183411110201 36.0 29.0 40.0 2.0 41.0 56-57 30.726026339932325 35.5 28.5 40.0 2.0 41.0 58-59 30.593320109331856 35.0 28.0 39.5 2.0 41.0 60-61 30.449564652321406 35.0 28.0 39.0 2.0 41.0 62-63 30.313933130062075 35.0 28.0 39.0 2.0 41.0 64-65 29.970888970078615 35.0 28.0 39.0 2.0 40.0 66-67 29.753002037049477 34.0 28.0 39.0 2.0 40.0 68-69 29.557481506382793 34.0 28.0 38.0 2.0 40.0 70-71 29.244679074160487 34.0 28.0 38.0 2.0 40.0 72-73 28.913816420805965 34.0 27.5 37.0 2.0 40.0 74-75 28.510987161981827 34.0 26.5 37.0 2.0 39.5 76-77 28.19999461991076 33.5 26.0 36.0 2.0 39.0 78-79 27.77030254035155 33.0 26.0 36.0 2.0 39.0 80-81 27.367906677605006 33.0 26.0 35.5 2.0 37.5 82-83 27.03161857347461 33.0 26.0 35.0 2.0 37.0 84-85 26.68855437002144 33.0 25.0 35.0 2.0 37.0 86-87 26.358465675909756 32.0 24.5 35.0 2.0 36.0 88-89 25.999628140890824 32.0 24.0 35.0 2.0 36.0 90-91 25.617284890494084 32.0 22.0 35.0 2.0 35.5 92-93 25.255416571870434 32.0 20.0 35.0 2.0 35.0 94-95 24.93940648121417 32.0 18.5 35.0 2.0 35.0 96-97 24.545370327717762 32.0 11.0 34.5 2.0 35.0 98-99 24.183049097006524 32.0 2.0 34.0 2.0 35.0 100-101 23.594635242256977 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.168894764575725 1101 2 5.859046181808704 1101 3 5.291558267342939 1101 4 6.682596244180786 1101 5 6.167225924016037 1101 6 5.710944500713595 1101 7 6.288968948529256 1101 8 5.8053431624583425 1101 9 6.220206441653016 1101 10-11 6.220307575935934 1101 12-13 6.097302890097179 1101 14-15 6.367951155920725 1101 16-17 6.437570432986817 1101 18-19 6.463676580235454 1101 20-21 5.805436895224357 1101 22-23 5.903795036874438 1101 24-25 5.942795212164118 1101 26-27 6.046891498117233 1101 28-29 5.890459031153984 1101 30-31 6.111984043189224 1101 32-33 5.8770431623067125 1101 34-35 5.663136415854851 1101 36-37 5.719273997563729 1101 38-39 5.551162124058045 1101 40-41 5.793680461900671 1101 42-43 5.606892860099254 1101 44-45 5.603166659855244 1101 46-47 5.448722937421369 1101 48-49 5.347753934634564 1101 50-51 5.312710168320791 1101 52-53 5.112450139151765 1101 54-55 5.370343034366083 1101 56-57 5.249628171127274 1101 58-59 5.203717832596791 1101 60-61 5.027830033357056 1101 62-63 4.939918378296451 1101 64-65 4.962454569883988 1101 66-67 4.891319306039172 1101 68-69 4.957281682211466 1101 70-71 4.7689267274261375 1101 72-73 4.541640244279783 1101 74-75 4.731247537435316 1101 76-77 4.627300279292498 1101 78-79 4.6141157749476776 1101 80-81 4.521026685054295 1101 82-83 4.383353062231613 1101 84-85 4.303365096662251 1101 86-87 4.196199920564329 1101 88-89 4.190548424614857 1101 90-91 4.144666301157638 1101 92-93 4.106496746700156 1101 94-95 4.078103935958712 1101 96-97 4.021245664105599 1101 98-99 3.6541136698303553 1101 100-101 3.666591483299456 1102 1 5.343945917112212 1102 2 5.9210587829149866 1102 3 5.655925772675062 1102 4 7.018137847413811 1102 5 6.5541529951254915 1102 6 6.14746844790789 1102 7 6.668669363420225 1102 8 6.180847438837702 1102 9 6.858609293761344 1102 10-11 6.7756813512991485 1102 12-13 6.795729791969929 1102 14-15 7.09263693549148 1102 16-17 7.107914965822033 1102 18-19 7.131845975653203 1102 20-21 6.425657926230091 1102 22-23 6.4939560310191915 1102 24-25 6.293287195388562 1102 26-27 6.410746955996885 1102 28-29 6.238632503674264 1102 30-31 6.571254865126988 1102 32-33 6.370392810475899 1102 34-35 6.312402906875185 1102 36-37 6.316876386572321 1102 38-39 6.131043092904832 1102 40-41 6.344123722303998 1102 42-43 6.326028125606495 1102 44-45 6.282998327568496 1102 46-47 6.303354914660279 1102 48-49 5.975202063625215 1102 50-51 5.969275805414647 1102 52-53 5.823172364613615 1102 54-55 6.148401467695262 1102 56-57 5.977425512464453 1102 58-59 6.056601981037385 1102 60-61 5.954879697060349 1102 62-63 5.858180143058561 1102 64-65 5.904863689292487 1102 66-67 5.809338621352609 1102 68-69 5.761877466731619 1102 70-71 5.74050568417945 1102 72-73 5.598052572358341 1102 74-75 5.607090287220899 1102 76-77 5.532355396481886 1102 78-79 5.322714830788399 1102 80-81 5.403679746278783 1102 82-83 5.244638563987515 1102 84-85 5.195182241023009 1102 86-87 5.210500221174335 1102 88-89 5.176963223372578 1102 90-91 5.177225089426056 1102 92-93 5.158857514371331 1102 94-95 5.109567504927046 1102 96-97 5.033328658684404 1102 98-99 4.891104692654579 1102 100-101 4.844785603179442 1103 1 5.13498205589536 1103 2 5.634292946517377 1103 3 5.365031990630303 1103 4 6.744419459824343 1103 5 6.453879163284256 1103 6 5.762073097940181 1103 7 6.283450230386752 1103 8 5.716858136900619 1103 9 6.24832547656035 1103 10-11 6.219279899380346 1103 12-13 6.293672575194872 1103 14-15 6.573091507221566 1103 16-17 6.345250692901978 1103 18-19 6.2695686044420675 1103 20-21 5.743327167844054 1103 22-23 5.855831637214639 1103 24-25 5.596948090204403 1103 26-27 5.807823813684365 1103 28-29 5.5913427505051025 1103 30-31 5.9006216850613455 1103 32-33 5.710306421802905 1103 34-35 5.828549802878648 1103 36-37 5.673735666716549 1103 38-39 5.587811438235015 1103 40-41 5.73335145079016 1103 42-43 5.6003723271698895 1103 44-45 5.393389082223916 1103 46-47 5.44016687608028 1103 48-49 5.169822853817447 1103 50-51 5.230315848314355 1103 52-53 5.000964417597661 1103 54-55 5.311999282605274 1103 56-57 5.389772239110972 1103 58-59 5.5039869145374745 1103 60-61 5.269740101506997 1103 62-63 5.334729638789494 1103 64-65 5.3395907061548655 1103 66-67 5.452522735827738 1103 68-69 5.334156152373957 1103 70-71 5.20395154396498 1103 72-73 5.120862971012379 1103 74-75 5.191746973218393 1103 76-77 5.108726468569245 1103 78-79 5.089163930039234 1103 80-81 5.095872536559604 1103 82-83 4.958695866152624 1103 84-85 4.889997997183546 1103 86-87 4.76401989858541 1103 88-89 4.7066890573268125 1103 90-91 4.709502970738832 1103 92-93 4.802507116591986 1103 94-95 4.722257434840838 1103 96-97 4.558775015619155 1103 98-99 4.4027773655416595 1103 100-101 4.341475079438567 1104 1 4.704456571388 1104 2 5.524275497415545 1104 3 5.152372563210541 1104 4 6.617272323567768 1104 5 6.246453435916315 1104 6 5.786621338612939 1104 7 6.383520918571005 1104 8 5.810572239163655 1104 9 6.403152694781131 1104 10-11 6.4059288692293705 1104 12-13 6.4069957224539955 1104 14-15 6.425098134114979 1104 16-17 6.364570128387371 1104 18-19 6.535403718852091 1104 20-21 5.940742719046423 1104 22-23 6.0940199626656515 1104 24-25 5.915449706797798 1104 26-27 6.012405763524882 1104 28-29 5.838317159479939 1104 30-31 6.2418801210233426 1104 32-33 6.1865651203436585 1104 34-35 5.897167102756477 1104 36-37 6.03736618966445 1104 38-39 5.873778931975238 1104 40-41 6.053757268742821 1104 42-43 6.023873443377344 1104 44-45 5.880251523651506 1104 46-47 5.912746994805513 1104 48-49 5.691534312457332 1104 50-51 5.616301336131201 1104 52-53 5.458599524278956 1104 54-55 5.756633431121809 1104 56-57 5.650661360522438 1104 58-59 5.547923574595085 1104 60-61 5.382703684309973 1104 62-63 5.3293267983809045 1104 64-65 5.445281431972106 1104 66-67 5.417619676933796 1104 68-69 5.362786690184805 1104 70-71 5.226594988760521 1104 72-73 5.224802583842472 1104 74-75 5.351208835387332 1104 76-77 5.219612666934122 1104 78-79 5.130216603338738 1104 80-81 5.031739447771322 1104 82-83 4.81082870920682 1104 84-85 4.888011970337097 1104 86-87 4.919675161406936 1104 88-89 4.871336978842518 1104 90-91 4.842898728456916 1104 92-93 4.896810723036953 1104 94-95 4.808970969339228 1104 96-97 4.732193028125835 1104 98-99 4.5662018483853615 1104 100-101 4.605228251439545 1105 1 4.597968192939632 1105 2 5.179849725101771 1105 3 4.9236075850311565 1105 4 6.285018791515867 1105 5 3.7860791261268147 1105 6 5.132762544313863 1105 7 5.829346289199275 1105 8 1.4255301194671013 1105 9 0.6852546341130186 1105 10-11 2.4034826689522717 1105 12-13 5.516364446118001 1105 14-15 3.7438775639029593 1105 16-17 5.892966023700922 1105 18-19 6.377275769011472 1105 20-21 3.4477407344861284 1105 22-23 5.4774628585245715 1105 24-25 5.664172177438122 1105 26-27 5.803104345453335 1105 28-29 3.3229608157488464 1105 30-31 5.703587511153522 1105 32-33 5.848926830621728 1105 34-35 5.821878780634776 1105 36-37 5.965450832413762 1105 38-39 5.8205632402253045 1105 40-41 5.972291940073141 1105 42-43 3.195039584368235 1105 44-45 5.27656427507787 1105 46-47 5.535797584982479 1105 48-49 5.367036341768678 1105 50-51 5.512537664682725 1105 52-53 5.489499903648596 1105 54-55 5.6083425277590315 1105 56-57 5.522142381896732 1105 58-59 5.43334511166865 1105 60-61 5.474766063705868 1105 62-63 5.189321905598472 1105 64-65 5.295593078345657 1105 66-67 5.419689085229333 1105 68-69 5.340637231195274 1105 70-71 5.171726777645308 1105 72-73 5.20117812995456 1105 74-75 5.1813085884808245 1105 76-77 5.164790209301437 1105 78-79 5.218882675711342 1105 80-81 5.0628306217885 1105 82-83 4.895830612618521 1105 84-85 4.8851273290198165 1105 86-87 4.871427147458924 1105 88-89 4.774382365963042 1105 90-91 4.9002407065014495 1105 92-93 4.977367378474089 1105 94-95 4.930661413735141 1105 96-97 4.888318254715358 1105 98-99 4.795962877731313 1105 100-101 4.845885292451804 1106 1 4.448032714576442 1106 2 4.581800356519757 1106 3 4.702440814252807 1106 4 6.275789696860663 1106 5 6.248925801842333 1106 6 5.429596370492057 1106 7 6.2221305265734905 1106 8 5.629351384605108 1106 9 6.242714249557057 1106 10-11 6.090640569691203 1106 12-13 6.395971494325057 1106 14-15 6.0595263251650415 1106 16-17 6.203992273967572 1106 18-19 6.407589337901609 1106 20-21 5.665050727477048 1106 22-23 5.797987640874876 1106 24-25 5.78753443587485 1106 26-27 5.899666339793612 1106 28-29 5.9026341535269715 1106 30-31 6.047158245672335 1106 32-33 5.847724529142951 1106 34-35 5.444869427784717 1106 36-37 5.663752320101235 1106 38-39 5.576728658772858 1106 40-41 5.736962821325193 1106 42-43 2.4405486331654522 1106 44-45 4.796646309351164 1106 46-47 5.010430621823151 1106 48-49 4.928076501908482 1106 50-51 5.2592130722712085 1106 52-53 5.066089762331718 1106 54-55 5.41485685583093 1106 56-57 5.2401307466594815 1106 58-59 5.13179769668054 1106 60-61 4.887356978265164 1106 62-63 4.979294952299224 1106 64-65 5.191530928182949 1106 66-67 5.150431051667038 1106 68-69 5.111528241392911 1106 70-71 4.997277961678137 1106 72-73 4.853146785807414 1106 74-75 5.024066789970327 1106 76-77 4.983107675292064 1106 78-79 4.991764045411244 1106 80-81 4.894936939727209 1106 82-83 4.8992297980521045 1106 84-85 4.913588734590199 1106 86-87 4.942287332952922 1106 88-89 5.086643117472079 1106 90-91 5.030398150338215 1106 92-93 4.967129844560812 1106 94-95 4.852239619448579 1106 96-97 4.909785807918766 1106 98-99 4.7672562708282555 1106 100-101 4.76782945524786 1107 1 2.78190040217007 1107 2 -0.26014669380504785 1107 3 0.45522424589825405 1107 4 2.234139396653042 1107 5 2.249508901227408 1107 6 3.608162799044454 1107 7 4.666586448794408 1107 8 4.129578106115705 1107 9 4.507700824809607 1107 10-11 2.2543466076817964 1107 12-13 4.530556217036146 1107 14-15 2.725238565122556 1107 16-17 1.2995104714862151 1107 18-19 1.953644270256408 1107 20-21 1.5455890380276962 1107 22-23 3.666663244336686 1107 24-25 3.7651801200325146 1107 26-27 2.7916511817602156 1107 28-29 3.645197546278343 1107 30-31 2.9618455627009936 1107 32-33 3.5157382049844763 1107 34-35 4.071606397957808 1107 36-37 4.23361981271437 1107 38-39 4.269654706750529 1107 40-41 2.8377505227034874 1107 42-43 3.3021066690543357 1107 44-45 3.8326729950397684 1107 46-47 4.154472394694675 1107 48-49 1.5240681222814487 1107 50-51 3.2886374150197213 1107 52-53 3.57632198750699 1107 54-55 4.014730302717734 1107 56-57 2.6461356217387646 1107 58-59 1.6902931925743268 1107 60-61 2.882545735258809 1107 62-63 3.77340369303694 1107 64-65 3.9496725684790697 1107 66-67 3.8221042960915312 1107 68-69 3.6737084577872565 1107 70-71 3.455796994098744 1107 72-73 3.5616789255653565 1107 74-75 3.841735650734414 1107 76-77 3.738738516559234 1107 78-79 3.636296670353218 1107 80-81 3.7077770574183475 1107 82-83 3.6858552410801053 1107 84-85 3.583432053022264 1107 86-87 3.5264943943077327 1107 88-89 3.601498335949426 1107 90-91 3.5281604798790624 1107 92-93 3.5334165543159024 1107 94-95 3.4996050588150247 1107 96-97 3.548795992815826 1107 98-99 3.399086114634141 1107 100-101 3.479732873017955 1108 1 -2.4792830121389464 1108 2 -1.8265596171734018 1108 3 -2.427041202332852 1108 4 -4.360322392763404 1108 5 -9.203893831482677 1108 6 -7.471864563136101 1108 7 -6.224167414359609 1108 8 -6.350566773122363 1108 9 -8.029558221372842 1108 10-11 -3.9059716530657553 1108 12-13 -2.497890803818745 1108 14-15 -7.145980718198793 1108 16-17 -6.615250197133221 1108 18-19 -5.0762208482253435 1108 20-21 -5.351518639168731 1108 22-23 -4.161273805108177 1108 24-25 -8.968895441681479 1108 26-27 -9.99755873527625 1108 28-29 -6.867977519057632 1108 30-31 -6.624757054246018 1108 32-33 -3.2123062760987864 1108 34-35 -1.6958426926004861 1108 36-37 -5.177873180111828 1108 38-39 -6.039746219354043 1108 40-41 -3.8830539429446986 1108 42-43 -4.806144957767618 1108 44-45 -2.3425181894269365 1108 46-47 -3.0201312044844606 1108 48-49 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-4.175159961029198 2313 44-45 -5.330906969596537 2313 46-47 -5.820344689629454 2313 48-49 -5.173014372688371 2313 50-51 -4.6242246055887755 2313 52-53 -3.4711232902199782 2313 54-55 -7.180637357790776 2313 56-57 -5.000411844879128 2313 58-59 -4.183917221424817 2313 60-61 -2.754282811781003 2313 62-63 -5.522826719742767 2313 64-65 -7.116196890700248 2313 66-67 -7.174206904445022 2313 68-69 -7.154683103702901 2313 70-71 -6.8597447411613 2313 72-73 -6.17880793499193 2313 74-75 -7.237860105621905 2313 76-77 -5.754772549839153 2313 78-79 -5.768177142219187 2313 80-81 -7.501082158483122 2313 82-83 -6.266086713678753 2313 84-85 -7.770424419144071 2313 86-87 -6.39405618187665 2313 88-89 -8.086502395713275 2313 90-91 -8.181124014968812 2313 92-93 -8.017873001199007 2313 94-95 -7.781960089651532 2313 96-97 -9.102703915726334 2313 98-99 -8.065454157300497 2313 100-101 -7.559078784807788 2314 1 -11.312657856472695 2314 2 -14.658460820992415 2314 3 -12.155040372112843 2314 4 -24.734170462501886 2314 5 -18.65802417602398 2314 6 -16.76586622357612 2314 7 -17.019414404812082 2314 8 -12.62146756183137 2314 9 -11.96664830024374 2314 10-11 -11.586285061118886 2314 12-13 -12.919123680531072 2314 14-15 -14.776916791258152 2314 16-17 -15.293041811911142 2314 18-19 -14.233402251297157 2314 20-21 -14.370053300854078 2314 22-23 -14.745084514946285 2314 24-25 -16.135396064346484 2314 26-27 -14.719435032495015 2314 28-29 -14.347055974848416 2314 30-31 -15.626801471016464 2314 32-33 -13.023036869706093 2314 34-35 -14.270967225601733 2314 36-37 -13.565419879987296 2314 38-39 -11.54885788394516 2314 40-41 -16.54017307951589 2314 42-43 -16.001350437219674 2314 44-45 -11.369002207691775 2314 46-47 -10.914392308677069 2314 48-49 -10.713490563164562 2314 50-51 -10.525415081779254 2314 52-53 -10.078266147362836 2314 54-55 -10.966351643505059 2314 56-57 -9.268268987736267 2314 58-59 -9.108917221424818 2314 60-61 -9.094759002257192 2314 62-63 -11.485921957838004 2314 64-65 -9.894768319271677 2314 66-67 -10.711111666349783 2314 68-69 -14.040397389417183 2314 70-71 -12.933554264970825 2314 72-73 -11.704998411182402 2314 74-75 -10.550955343717145 2314 76-77 -10.815486835553443 2314 78-79 -10.930081904123949 2314 80-81 -10.50465358705455 2314 82-83 -10.957753380345418 2314 84-85 -11.021614895334546 2314 86-87 -11.894056181876646 2314 88-89 -11.229359538570419 2314 90-91 -10.578743062587861 2314 92-93 -10.728587286913292 2314 94-95 -10.385531518222962 2314 96-97 -10.226513439535857 2314 98-99 -10.078549395395735 2314 100-101 -9.501935927664931 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 105172.0 3 17780.0 4 12167.0 5 9544.0 6 9839.0 7 11441.0 8 13194.0 9 15092.0 10 16774.0 11 18075.0 12 19338.0 13 20632.0 14 23051.0 15 25637.0 16 27530.0 17 29242.0 18 27418.0 19 25316.0 20 25413.0 21 26791.0 22 31429.0 23 37815.0 24 46080.0 25 55488.0 26 65793.0 27 74291.0 28 82917.0 29 91394.0 30 102980.0 31 113159.0 32 129375.0 33 152893.0 34 183316.0 35 222092.0 36 270963.0 37 325053.0 38 299349.0 39 79893.0 40 93.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.9513197271158 20.381218931068705 14.399626266986226 23.26783507482927 2 14.528864719299037 20.55861439185222 41.47742028363669 23.43510060521205 3 17.729539045909743 25.98154101931241 30.935794436987727 25.353125497790117 4 11.282039148492565 17.73734863940113 40.506001452821614 30.474610759284694 5 12.209465994190111 37.93306826529588 36.13764042469607 13.719825315817937 6 29.274991464483453 38.58908791406055 18.85145385365373 13.284466767802272 7 25.652915754497833 32.772250495202506 23.42527009525982 18.149563655039834 8 24.368115843052575 35.924505160928526 21.415609199375783 18.291769796643116 9 24.902533341077863 16.41734563305365 21.904590921651767 36.77553010421672 10-11 23.268925460780128 27.033103073839253 29.891599994225615 19.806371471155007 12-13 24.27444652996626 24.617133948210995 29.459123821346022 21.64929570047672 14-15 21.596346287268112 26.223043297087383 27.356228717234245 24.824381698410257 16-17 20.853749087694357 29.243475496341798 28.077185370296633 21.825590045667212 18-19 20.731372600454886 28.32865059989398 30.028906247386704 20.91107055226443 20-21 21.721484239760724 27.669827638251366 29.881328477557172 20.72735964443074 22-23 21.375340011387248 27.512168249646457 29.70024437699706 21.412247361969236 24-25 21.194056695703164 27.606443255107788 29.79770541443842 21.40179463475063 26-27 21.10160723600369 27.8293696558307 29.673808392512253 21.395214715653353 28-29 21.015464164312732 27.877440654051632 29.837620642166957 21.269474539468682 30-31 21.29396742749915 27.651821299284034 29.78837609828895 21.26583517492786 32-33 21.189471149818473 27.774226500025694 29.607436751078346 21.428865599077483 34-35 21.45878807984803 27.8817634409511 29.630120718691426 21.029327760509442 36-37 21.308181243086107 27.52901700392997 29.65351787871409 21.509283874269833 38-39 21.221517875283055 27.93268480832456 29.601796912164613 21.244000404227773 40-41 21.389092181489776 27.619332756721594 29.615314075627662 21.376260986160965 42-43 21.25391077062533 27.849667997025396 29.51194462656816 21.38447660578112 44-45 21.43136319457875 27.977764117970754 29.22912640802235 21.361746279428147 46-47 21.405589095289507 27.850937514023773 29.12070168584459 21.62277170484213 48-49 21.38794164275476 27.978351866817974 29.194919788355023 21.43878670207224 50-51 21.234384153218265 28.172709466107104 28.97575108191095 21.617155298763684 52-53 21.308515687570935 28.331110935517145 28.821598855815626 21.538774521096297 54-55 21.364494027878425 28.123087059630215 28.77998780656206 21.7324311059293 56-57 21.377044229729865 28.1954473421662 28.64041877339866 21.787089654705273 58-59 21.27067534673402 28.347616445084086 28.834344247632426 21.54736396054947 60-61 21.467138725687192 28.327169023028727 28.615631272689107 21.590060978594973 62-63 21.351446769291577 28.247156376689237 28.66777034684697 21.73362650717222 64-65 21.1972527084178 28.150349934366425 28.816285424634973 21.836111932580803 66-67 21.295694275901525 28.158296994288314 28.613582650653928 21.932426079156233 68-69 21.35904218939391 28.104074134113315 28.570682592668522 21.966201083824252 70-71 21.32876600093044 27.89486602347055 28.64352478128882 22.132843194310187 72-73 21.451716863836975 27.974582770563106 28.69064451710886 21.88305584849106 74-75 21.276072773970494 28.241829033423016 28.527184746989874 21.954913445616615 76-77 21.243721910571665 28.124205513782698 28.51596743674615 22.116105138899485 78-79 21.34534581842234 28.151246615906288 28.384137668395915 22.11926989727546 80-81 21.296256899612807 28.18213817405397 28.387249680798952 22.134355245534262 82-83 21.423427440353976 28.077982459502522 28.5615047933782 21.937085306765304 84-85 21.531961070658856 28.037332896362248 28.323180905676487 22.107525127302406 86-87 21.218386261572906 28.076822048098 28.499651349118913 22.205140341210182 88-89 21.487601707422307 27.981246345143624 28.334116904064572 22.1970350433695 90-91 21.39348530971908 27.926882829040807 28.45806994045683 22.221561920783284 92-93 21.477263496727463 27.916720438255737 28.433437571097176 22.17257849391962 94-95 21.264591733862108 27.88619810191858 28.44822402550936 22.400986138709953 96-97 21.241488997717507 27.781761075511486 28.53140442482451 22.445345501946502 98-99 21.246675685055905 27.831799421833807 28.246541007005018 22.674983886105267 100-101 21.306454454379832 27.72004828718002 28.234778655040987 22.73871860339916 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 258.0 1 190.5 2 144.5 3 164.0 4 165.5 5 185.0 6 283.0 7 533.5 8 922.5 9 1207.0 10 1257.0 11 1307.5 12 1427.5 13 1589.5 14 1838.0 15 2146.0 16 2436.5 17 2770.5 18 3080.0 19 3506.5 20 4142.0 21 5020.0 22 6250.0 23 8033.5 24 10516.0 25 13879.0 26 18713.5 27 24862.0 28 31281.0 29 38233.5 30 45469.5 31 53057.5 32 62014.5 33 70116.0 34 78500.5 35 86675.5 36 93912.5 37 100148.0 38 102396.5 39 103023.5 40 104858.0 41 105597.0 42 104382.0 43 103967.5 44 104124.5 45 104355.5 46 103165.5 47 100846.5 48 99053.5 49 96485.0 50 92846.5 51 88547.5 52 83937.5 53 78906.0 54 73225.0 55 66939.5 56 60549.0 57 53591.0 58 46957.0 59 41029.5 60 34379.5 61 27648.0 62 21369.0 63 16166.0 64 12266.0 65 9168.5 66 6715.5 67 5058.5 68 3999.0 69 3105.0 70 2320.5 71 1685.5 72 1243.0 73 929.5 74 703.5 75 534.0 76 398.5 77 274.0 78 194.0 79 152.0 80 125.5 81 93.5 82 73.0 83 65.5 84 50.5 85 44.5 86 38.0 87 31.0 88 28.0 89 21.0 90 14.5 91 8.5 92 6.0 93 5.0 94 4.5 95 4.0 96 1.5 97 0.5 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04036825128462817 2 0.3435872676847577 3 0.0 4 0.42506221387507437 5 0.19530075577946418 6 0.09666578639498506 7 0.10767211274697862 8 0.11069621519513022 9 0.10914899998909916 10-11 0.12975509341487626 12-13 0.17351666895818615 14-15 0.1279793123261361 16-17 0.12265196905991554 18-19 0.13276161387205024 20-21 0.10825231844924027 22-23 0.10273157328226584 24-25 0.09633172856641017 26-27 0.12773316445244934 28-29 0.11206761049138501 30-31 0.12878808391110685 32-33 0.1020107116521832 34-35 0.15208422195646065 36-37 0.13979441026310044 38-39 0.13488903478034292 40-41 0.10851604831390466 42-43 0.1092369099439873 44-45 0.08986155588664399 46-47 0.0935889379739006 48-49 0.09968988884313665 50-51 0.08560671407005861 52-53 0.09383508584758735 54-55 0.30548709323624323 56-57 0.3254426529958482 58-59 0.3370819310230363 60-61 0.16266858052499122 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2843819.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.2438859281348 #Duplication Level Percentage of deduplicated Percentage of total 1 84.02330380128643 59.0212336752258 2 10.19530435068586 14.323155916243879 3 2.7821836681211294 5.862941766438609 4 1.0911508317022431 3.065866982099272 5 0.5478880263934666 1.9242892013686785 6 0.2933694985222155 1.236444815339317 7 0.19447803416824566 0.9562624993349508 8 0.12906059933168015 0.7252574413817002 9 0.1073155612264857 0.6784435836996325 >10 0.577342549189945 7.482275865293242 >50 0.04114402840020851 1.9755267266604406 >100 0.01674175423890369 2.0002176941992826 >500 5.123548094085593E-4 0.23184624512175836 >1k 1.5370644278063953E-4 0.300730708444685 >5k 5.123548092687983E-5 0.21550687914872832 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5988 0.2105619239480431 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4570 0.16069939753549717 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.516398195525102E-5 0.0 5 3.516398195525102E-5 0.0 0.0 3.516398195525102E-5 0.0 6 3.516398195525102E-5 0.0 0.0 3.516398195525102E-5 0.0 7 3.516398195525102E-5 0.0 0.0 3.516398195525102E-5 0.0 8 3.516398195525102E-5 0.0 0.0 3.516398195525102E-5 0.0 9 3.516398195525102E-5 0.0 0.0 7.032796391050204E-5 0.0 10-11 3.516398195525102E-5 1.758199097762551E-5 0.0 8.790995488812755E-5 0.0 12-13 3.516398195525102E-5 3.516398195525102E-5 0.0 1.0549194586575305E-4 0.0 14-15 3.516398195525102E-5 5.274597293287653E-5 0.0 1.2307393684337856E-4 0.0 16-17 3.516398195525102E-5 7.032796391050204E-5 0.0 1.582379187986296E-4 0.0 18-19 3.516398195525102E-5 7.032796391050204E-5 0.0 2.2856588270913164E-4 1.758199097762551E-5 20-21 3.516398195525102E-5 7.032796391050204E-5 0.0 2.6372986466438266E-4 3.516398195525102E-5 22-23 3.516398195525102E-5 7.032796391050204E-5 0.0 2.8131185564200816E-4 3.516398195525102E-5 24-25 7.032796391050204E-5 7.032796391050204E-5 0.0 3.868038015077612E-4 3.516398195525102E-5 26-27 7.032796391050204E-5 7.032796391050204E-5 0.0 8.439355669260245E-4 3.516398195525102E-5 28-29 7.032796391050204E-5 7.032796391050204E-5 0.0 0.0023559867910018183 5.274597293287653E-5 30-31 7.032796391050204E-5 7.032796391050204E-5 0.0 0.0073141082466922115 7.032796391050204E-5 32-33 7.032796391050204E-5 7.032796391050204E-5 0.0 0.019920395777649702 7.032796391050204E-5 34-35 7.032796391050204E-5 7.032796391050204E-5 0.0 0.041317678797419946 7.032796391050204E-5 36-37 7.032796391050204E-5 1.0549194586575305E-4 0.0 0.07516301142934906 7.032796391050204E-5 38-39 7.032796391050204E-5 1.582379187986296E-4 0.0 0.12099926190801877 7.032796391050204E-5 40-41 7.032796391050204E-5 1.758199097762551E-4 0.0 0.16908600723182454 7.032796391050204E-5 42-43 7.032796391050204E-5 1.758199097762551E-4 0.0 0.22271107971358234 7.032796391050204E-5 44-45 7.032796391050204E-5 1.758199097762551E-4 0.0 0.2867446908540945 7.032796391050204E-5 46-47 8.790995488812755E-5 1.758199097762551E-4 0.0 0.36505487866843844 7.032796391050204E-5 48-49 1.0549194586575305E-4 1.758199097762551E-4 0.0 0.44594961915649345 7.032796391050204E-5 50-51 1.0549194586575305E-4 1.934019007538806E-4 0.0 0.528743214670132 7.032796391050204E-5 52-53 1.0549194586575305E-4 2.109838917315061E-4 0.0 0.6126620576063385 7.032796391050204E-5 54-55 1.0549194586575305E-4 2.109838917315061E-4 0.0 0.7066202173907692 7.032796391050204E-5 56-57 1.0549194586575305E-4 2.109838917315061E-4 0.0 0.8172636866129666 7.032796391050204E-5 58-59 1.0549194586575305E-4 2.109838917315061E-4 0.0 0.9421837325089959 7.032796391050204E-5 60-61 1.0549194586575305E-4 2.4614787368675716E-4 0.0 1.0712179642937896 8.790995488812755E-5 62-63 1.0549194586575305E-4 2.4614787368675716E-4 0.0 1.1992324406018808 1.0549194586575305E-4 64-65 1.0549194586575305E-4 2.4614787368675716E-4 0.0 1.33674119203789 1.0549194586575305E-4 66-67 1.0549194586575305E-4 2.4614787368675716E-4 0.0 1.4943109951793696 1.0549194586575305E-4 68-69 1.0549194586575305E-4 2.4614787368675716E-4 0.0 1.6737703770879933 1.0549194586575305E-4 70-71 1.0549194586575305E-4 2.4614787368675716E-4 0.0 1.8635679696914607 1.0549194586575305E-4 72-73 1.0549194586575305E-4 2.4614787368675716E-4 0.0 2.0582357737957304 1.0549194586575305E-4 74-75 1.0549194586575305E-4 2.4614787368675716E-4 0.0 2.254274973196255 1.0549194586575305E-4 76-77 1.0549194586575305E-4 2.4614787368675716E-4 0.0 2.470322478329317 1.0549194586575305E-4 78-79 1.0549194586575305E-4 2.4614787368675716E-4 0.0 2.711793542416026 1.0549194586575305E-4 80-81 1.0549194586575305E-4 2.4614787368675716E-4 0.0 2.9735718060818916 1.4065592782100408E-4 82-83 1.4065592782100408E-4 2.4614787368675716E-4 0.0 3.243244383696712 1.758199097762551E-4 84-85 1.758199097762551E-4 2.4614787368675716E-4 0.0 3.5115455660152772 1.758199097762551E-4 86-87 1.758199097762551E-4 2.4614787368675716E-4 0.0 3.7995737422107387 1.758199097762551E-4 88-89 1.758199097762551E-4 2.8131185564200816E-4 0.0 4.107381658256028 1.758199097762551E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3505 0.0 50.1943 1 GTATCAA 6640 0.0 44.927383 1 ATCAACG 8165 0.0 35.142155 3 TCAACGC 8290 0.0 34.84656 4 CAACGCA 8300 0.0 34.376747 5 AACGCAG 8420 0.0 34.14633 6 TATCAAC 8675 0.0 34.002457 2 ACGCAGA 9380 0.0 29.648262 7 CGCAGAG 9365 0.0 29.237343 8 GCAGAGT 10345 0.0 24.905754 9 GTACATG 6960 0.0 22.117765 1 GAGTACT 6395 0.0 21.740538 12-13 GTGGTAT 1750 0.0 21.581602 1 AGAGTAC 9185 0.0 21.477917 10-11 TACATGG 6940 0.0 21.21709 2 CAGAGTA 10305 0.0 20.619719 10-11 ACATGGG 6960 0.0 20.339268 3 CCGTCGA 330 2.7284841E-11 20.16717 9 TGGTATC 1985 0.0 19.0266 2 GTACTTT 7425 0.0 18.974724 14-15 >>END_MODULE