FastQCFastQC Report
Sat 18 Jun 2016
SRR1931125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2843819
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77200.2714659406945379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61910.21770021228495906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40310.14174601126161684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39850.053.4386521
GTATCAA74400.041.5923921
ATCAACG90650.033.7379723
TCAACGC92700.033.143824
CAACGCA94000.032.533325
TATCAAC95950.032.0228652
AACGCAG97600.031.4792846
ACGCAGA111850.026.959277
CGCAGAG111550.026.7337848
GTGGTAT17300.024.7287541
GCAGAGT125600.022.9492999
TGGTATC18700.021.8402582
GTACATG76250.021.382581
AGAGTAC112550.021.05355510-11
GAGTACT81350.020.6640812-13
TACATGG77600.020.5014252
CTAACGC2401.4182115E-719.7874453
ACATGGG76100.019.719843
CAGAGTA124650.019.5431910-11
AGTACTT87450.019.22267512-13
Produced by FastQC (version 0.11.5)