##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931125_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2843819 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28371425888919 31.0 31.0 34.0 30.0 34.0 2 31.288653743434445 31.0 31.0 34.0 28.0 34.0 3 31.44584201737171 31.0 31.0 34.0 28.0 34.0 4 35.0818199048533 35.0 35.0 37.0 32.0 37.0 5 34.9536489488255 35.0 35.0 37.0 32.0 37.0 6 35.08002232209574 36.0 35.0 37.0 32.0 37.0 7 34.93004934561588 36.0 35.0 37.0 32.0 37.0 8 34.982623366677 36.0 35.0 37.0 32.0 37.0 9 36.39008952398166 38.0 35.0 39.0 32.0 39.0 10-11 36.253060057619706 38.0 35.0 39.0 32.0 39.0 12-13 36.18387474730284 38.0 35.0 39.0 32.0 39.0 14-15 37.158395101797964 39.0 36.0 40.0 31.5 41.0 16-17 37.196035507182415 39.0 36.0 40.0 31.5 41.0 18-19 37.262262999157116 39.0 36.0 40.0 31.5 41.0 20-21 37.2359207811749 39.0 36.0 40.0 31.0 41.0 22-23 37.129413299510276 39.0 36.0 40.0 31.0 41.0 24-25 37.00274296641242 39.0 36.0 40.0 31.0 41.0 26-27 36.76191593065522 39.0 36.0 40.0 30.5 41.0 28-29 36.47596823145214 38.0 35.0 40.0 30.0 41.0 30-31 36.26250774047153 38.0 35.0 40.0 30.0 41.0 32-33 36.130583381009835 38.0 35.0 40.0 30.0 41.0 34-35 35.90525908997725 38.0 34.5 40.0 29.0 41.0 36-37 36.12789562204908 38.0 35.0 40.0 30.0 41.0 38-39 36.114010772134236 38.0 35.0 40.0 30.0 41.0 40-41 35.96247088861844 38.0 35.0 40.0 29.0 41.0 42-43 35.8301644724928 38.0 35.0 40.0 29.0 41.0 44-45 35.587094150506765 38.0 34.0 40.0 27.5 41.0 46-47 35.39761549521963 38.0 34.0 40.0 27.0 41.0 48-49 35.15695830149528 38.0 34.0 40.0 26.5 41.0 50-51 34.819101532129864 38.0 33.0 40.0 26.0 41.0 52-53 34.69976077943076 38.0 33.0 40.0 25.5 41.0 54-55 34.474372489951016 38.0 33.0 40.0 24.5 41.0 56-57 34.26738445730899 38.0 33.0 40.0 24.0 41.0 58-59 34.039508140286 37.0 33.0 40.0 24.0 41.0 60-61 33.776318042744634 37.0 32.0 40.0 23.0 41.0 62-63 33.52097584269604 37.0 32.0 40.0 22.5 41.0 64-65 33.28710230855058 36.0 32.0 39.0 22.0 41.0 66-67 33.05035112994182 36.0 31.5 39.0 22.0 41.0 68-69 32.74742837008966 35.5 31.0 39.0 22.0 40.0 70-71 31.93558749695392 35.0 30.5 38.5 18.5 40.0 72-73 31.894296191142967 35.0 31.0 38.0 20.0 40.0 74-75 31.582889065724647 35.0 30.5 37.0 19.5 39.5 76-77 30.85345357774176 34.0 29.5 36.0 18.0 39.0 78-79 30.876319308647986 34.0 30.0 36.0 18.0 39.0 80-81 30.59746418460528 34.0 30.0 36.0 17.0 38.5 82-83 30.236026624760576 34.0 30.0 36.0 13.5 37.0 84-85 29.8762044982469 34.0 29.0 35.0 9.5 37.0 86-87 29.534495163018462 34.0 29.0 35.0 7.0 36.5 88-89 29.207429868075288 34.0 29.0 35.0 4.5 36.0 90-91 28.90479914509327 34.0 29.0 35.0 2.0 36.0 92-93 28.56106278212502 34.0 29.0 35.0 2.0 35.5 94-95 28.177831817003824 33.5 29.0 35.0 2.0 35.0 96-97 27.813767331887156 33.0 27.5 35.0 2.0 35.0 98-99 27.34204778855476 33.0 27.0 35.0 2.0 35.0 100-101 26.669620148117723 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7840830678186208 1101 2 0.7180987173235387 1101 3 0.5810566196576232 1101 4 0.7639278828684297 1101 5 0.8432099191258118 1101 6 0.9111624373337293 1101 7 0.8298732829731179 1101 8 0.7853014688102817 1101 9 1.2385860667932036 1101 10-11 0.939460362953497 1101 12-13 1.0532238519533124 1101 14-15 1.3144832491590108 1101 16-17 1.1046589361825667 1101 18-19 0.9543244703697269 1101 20-21 0.8361188286036025 1101 22-23 0.7304395370702679 1101 24-25 0.6502311333369022 1101 26-27 0.9049609350490471 1101 28-29 0.7095833045438908 1101 30-31 1.0305490620414375 1101 32-33 1.193418111933589 1101 34-35 0.8586391754749698 1101 36-37 1.2237089428594032 1101 38-39 1.0159891412474238 1101 40-41 1.1065301271387114 1101 42-43 1.1947286310986485 1101 44-45 1.1300950149640343 1101 46-47 1.1886922327797862 1101 48-49 1.016166277811429 1101 50-51 0.9740425436553082 1101 52-53 0.7947117989302726 1101 54-55 0.8388791784549667 1101 56-57 0.6793072989276467 1101 58-59 0.8272338851090382 1101 60-61 0.9823067387306921 1101 62-63 0.881485253224568 1101 64-65 0.6674523673598358 1101 66-67 0.4635893611687223 1101 68-69 0.5531931700080932 1101 70-71 0.7356751100820453 1101 72-73 0.27551560739468783 1101 74-75 -0.025940842381395157 1101 76-77 0.1171457691360942 1101 78-79 0.18070893047472936 1101 80-81 0.16351105401160382 1101 82-83 0.16816582163418659 1101 84-85 0.2709682183530653 1101 86-87 0.20327161269036864 1101 88-89 0.18560922348676456 1101 90-91 0.15193014889166534 1101 92-93 0.02183088184650117 1101 94-95 0.03965559727021173 1101 96-97 0.004521334738697647 1101 98-99 -0.035199223385657064 1101 100-101 -0.1165578120429629 1102 1 0.9935389095692138 1102 2 0.8765420223854292 1102 3 0.6601499992816535 1102 4 0.8804384774715857 1102 5 0.9561141735614314 1102 6 0.905907906778026 1102 7 0.9718967724170682 1102 8 0.98398077995666 1102 9 1.4721060504590966 1102 10-11 1.2433013292878385 1102 12-13 1.2701229359546318 1102 14-15 1.5632240541923892 1102 16-17 1.4331080597099444 1102 18-19 1.3157372008573418 1102 20-21 1.3747136724022226 1102 22-23 0.9092840977769328 1102 24-25 0.7760949272687725 1102 26-27 1.092217170341243 1102 28-29 1.1080236521803286 1102 30-31 1.4528106202174982 1102 32-33 1.6506107039443023 1102 34-35 1.5805513847585217 1102 36-37 1.7337703740958972 1102 38-39 1.478050481239059 1102 40-41 1.6067576368499417 1102 42-43 1.7947806952804015 1102 44-45 1.7409794329624617 1102 46-47 1.6641445574270293 1102 48-49 1.4616733847087815 1102 50-51 1.369828276655852 1102 52-53 1.3651549099362228 1102 54-55 1.1983747229594002 1102 56-57 1.2903607929868528 1102 58-59 1.5035924654741422 1102 60-61 1.5172074914846263 1102 62-63 1.6032265792210438 1102 64-65 1.4559760064421212 1102 66-67 1.1358570639142087 1102 68-69 1.1497684119073384 1102 70-71 1.5104125441966012 1102 72-73 1.0405832562928623 1102 74-75 1.041248417907223 1102 76-77 1.0656213857978543 1102 78-79 0.9745004334267726 1102 80-81 0.8515137139274387 1102 82-83 0.958385602842533 1102 84-85 1.0876528608973537 1102 86-87 0.9933516752252167 1102 88-89 0.9415763232933685 1102 90-91 0.8675922467295933 1102 92-93 0.718367826612365 1102 94-95 0.68569647046413 1102 96-97 0.7467664865127759 1102 98-99 0.7785543792195782 1102 100-101 0.5818968809542682 1103 1 0.7286694664091264 1103 2 0.4708697719712518 1103 3 0.4529323167598065 1103 4 0.7299271485376551 1103 5 0.7495377559765473 1103 6 0.6984943388256539 1103 7 0.7843282774783873 1103 8 0.6205824443751737 1103 9 0.9517144171176 1103 10-11 0.8131356645818144 1103 12-13 0.8893857415249116 1103 14-15 1.2245520366819918 1103 16-17 1.0179708851406062 1103 18-19 0.9425011391651097 1103 20-21 0.9549456463253136 1103 22-23 0.7489233695596482 1103 24-25 0.47982522948960593 1103 26-27 0.8490527123694136 1103 28-29 0.7454802165849443 1103 30-31 1.1307125889140508 1103 32-33 1.2285222950129153 1103 34-35 0.9485984413960367 1103 36-37 1.1936862984700412 1103 38-39 0.9862918610208524 1103 40-41 1.0503215851678576 1103 42-43 1.2890006159332117 1103 44-45 1.2212521310668052 1103 46-47 1.3263368743846868 1103 48-49 1.2017446318377623 1103 50-51 0.9936849953382278 1103 52-53 0.708849989558054 1103 54-55 0.8714529129876141 1103 56-57 0.8598094938307028 1103 58-59 1.1477536484814266 1103 60-61 1.1758919601126152 1103 62-63 1.0608948382097267 1103 64-65 0.9222538298687581 1103 66-67 0.7589554861090377 1103 68-69 0.7480165285488809 1103 70-71 1.036523252565491 1103 72-73 0.601645342300003 1103 74-75 0.424648574823447 1103 76-77 0.5995007904116818 1103 78-79 0.6650634769138968 1103 80-81 0.6025175667939742 1103 82-83 0.5267644094461303 1103 84-85 0.734420139651359 1103 86-87 0.736169515926548 1103 88-89 0.6318942501443132 1103 90-91 0.5650095013257257 1103 92-93 0.28818864980929604 1103 94-95 0.2087362149783587 1103 96-97 0.18576756356283042 1103 98-99 0.14422506574844007 1103 100-101 -0.07121860486039111 1104 1 0.5143598949673915 1104 2 0.43706833502136533 1104 3 0.31711175813092396 1104 4 0.6645086524897081 1104 5 0.4875979429920747 1104 6 0.662304326925387 1104 7 0.6363764215419749 1104 8 0.5574886720497858 1104 9 0.9955548841709074 1104 10-11 0.7487966347756583 1104 12-13 0.6820489342250227 1104 14-15 1.099015330648328 1104 16-17 1.0526750381457433 1104 18-19 0.8252190968770492 1104 20-21 0.8411804353358505 1104 22-23 0.5888587256412166 1104 24-25 0.6149084669569191 1104 26-27 0.4687782828837044 1104 28-29 0.7547785169572094 1104 30-31 0.8864683252691208 1104 32-33 1.1820399487280113 1104 34-35 0.9564588406544701 1104 36-37 1.1319626323949237 1104 38-39 0.6963657695106704 1104 40-41 1.0602353107046767 1104 42-43 1.2239275942659944 1104 44-45 1.1261832412993442 1104 46-47 1.0006201681330253 1104 48-49 0.7875164895379072 1104 50-51 0.6944999803613285 1104 52-53 0.34051298507637995 1104 54-55 0.7499838591332022 1104 56-57 0.7682594170883448 1104 58-59 0.9150548045215814 1104 60-61 0.9252465415174811 1104 62-63 0.14382589097220233 1104 64-65 -0.6879950056192072 1104 66-67 -0.08855513842215856 1104 68-69 0.3816605569628706 1104 70-71 0.9459981049516379 1104 72-73 0.5288699640627286 1104 74-75 0.24537263823473054 1104 76-77 0.14270859127987734 1104 78-79 0.0669083057472264 1104 80-81 0.08937560878873896 1104 82-83 -0.06264417400586098 1104 84-85 -0.05506434392557935 1104 86-87 0.10404487722006195 1104 88-89 0.15869182966356377 1104 90-91 0.2830175689343051 1104 92-93 0.3333673173162133 1104 94-95 0.2944187562651521 1104 96-97 0.419784216010612 1104 98-99 0.44930012859394 1104 100-101 0.0877900166407457 1105 1 -0.28191051599053196 1105 2 -1.7158601384663186 1105 3 -0.2716684473420692 1105 4 0.3734991760054953 1105 5 0.59667863690796 1105 6 0.127631898709069 1105 7 -0.09344332119313492 1105 8 -0.09713358724620491 1105 9 0.6879706179271636 1105 10-11 0.8970099935037226 1105 12-13 1.0041058838257868 1105 14-15 1.2230108722495032 1105 16-17 0.934923975755602 1105 18-19 0.7245596452744252 1105 20-21 1.0847728116648057 1105 22-23 0.3742987598190979 1105 24-25 -0.09548447120666026 1105 26-27 1.0014222828407497 1105 28-29 0.4816991309656018 1105 30-31 0.9956062050713541 1105 32-33 1.1621316227636882 1105 34-35 0.6280942830157059 1105 36-37 0.8878294723376925 1105 38-39 0.7792023660912051 1105 40-41 1.0568199576307578 1105 42-43 1.457573874736994 1105 44-45 1.3667569140724396 1105 46-47 1.3928357035525991 1105 48-49 1.271962047786488 1105 50-51 0.8595284002670809 1105 52-53 1.0146317889939453 1105 54-55 0.274035082985165 1105 56-57 0.5059682583461935 1105 58-59 -0.09873467933761049 1105 60-61 0.6007079010665777 1105 62-63 1.0272706962096336 1105 64-65 1.4030849269319958 1105 66-67 1.0964007251002386 1105 68-69 1.1563771156899847 1105 70-71 0.5222101366616378 1105 72-73 0.06945697861815603 1105 74-75 0.17087472721415864 1105 76-77 0.34790821083620926 1105 78-79 -0.32092031321123216 1105 80-81 -0.08525739076136318 1105 82-83 -0.01971146408460811 1105 84-85 0.09313443192412407 1105 86-87 -0.07750188644303435 1105 88-89 -0.41454837373298403 1105 90-91 -0.5749485133431982 1105 92-93 -0.15492895617361668 1105 94-95 -0.3874897391565142 1105 96-97 -0.44241030431174266 1105 98-99 -1.008801233150944 1105 100-101 -0.41852078960626216 1106 1 0.39591797442402665 1106 2 0.3758978263429782 1106 3 -0.22941338507733633 1106 4 0.4243248437776259 1106 5 0.058718964709520094 1106 6 0.51732840969877 1106 7 0.5792824756010333 1106 8 0.5607795219173184 1106 9 1.0789793290117728 1106 10-11 0.2510076910480805 1106 12-13 0.4537854045956209 1106 14-15 0.14091103142730077 1106 16-17 -0.08989484814656379 1106 18-19 0.5692529118658456 1106 20-21 0.5928442279266832 1106 22-23 0.5247308259629904 1106 24-25 0.4438572159080536 1106 26-27 0.7856198387167623 1106 28-29 0.6146064027605931 1106 30-31 0.17457572981346203 1106 32-33 0.33031603179210833 1106 34-35 -0.9741410813820934 1106 36-37 -0.6381145381930367 1106 38-39 -0.7026501072806752 1106 40-41 0.5261856387457513 1106 42-43 0.8238303381825389 1106 44-45 0.5723891969130435 1106 46-47 0.17986187460729752 1106 48-49 0.05047176627507355 1106 50-51 0.6415139146738369 1106 52-53 -0.002227693998371194 1106 54-55 0.63108902388052 1106 56-57 0.3724359448083092 1106 58-59 0.8526994498209746 1106 60-61 0.9091032209357905 1106 62-63 0.7678196010407206 1106 64-65 0.8225526544866355 1106 66-67 0.5909976950201852 1106 68-69 0.6952108004334363 1106 70-71 1.0783614542080109 1106 72-73 0.48822273539860817 1106 74-75 0.5067299948778121 1106 76-77 0.5107194004478472 1106 78-79 0.41667131011449854 1106 80-81 0.5297964792042613 1106 82-83 0.46636159396702226 1106 84-85 0.6779373805199214 1106 86-87 0.6673854122606606 1106 88-89 0.6635513963718545 1106 90-91 0.7438640007856776 1106 92-93 0.2891862967880421 1106 94-95 0.46036584684148707 1106 96-97 0.7587179669108224 1106 98-99 0.778238900868029 1106 100-101 0.5422598983081102 1107 1 0.41360196884894407 1107 2 0.3679298405538809 1107 3 0.18494964934068747 1107 4 -1.3124938509979742 1107 5 -0.01081581408680421 1107 6 0.20758318232827122 1107 7 0.5023956850197209 1107 8 0.39968396956987107 1107 9 0.8364804101899779 1107 10-11 0.6416788944121734 1107 12-13 0.7981617759065074 1107 14-15 0.960326285477251 1107 16-17 0.8829888340828305 1107 18-19 0.7320955660038777 1107 20-21 0.18672712569964744 1107 22-23 0.3250447927448832 1107 24-25 0.2986608637437911 1107 26-27 0.740856368766714 1107 28-29 0.6753497007310401 1107 30-31 0.8816028638438453 1107 32-33 1.185586938491106 1107 34-35 0.9778344848407059 1107 36-37 1.1654946840444893 1107 38-39 0.9044434986626015 1107 40-41 0.3258221333137854 1107 42-43 0.2697303347250326 1107 44-45 -0.06067316170484105 1107 46-47 0.4028872577808187 1107 48-49 0.6848751826281543 1107 50-51 -0.7973108120275612 1107 52-53 0.527901681797637 1107 54-55 1.0893342900770833 1107 56-57 0.9707042034000679 1107 58-59 1.1911497459476124 1107 60-61 1.3257922763516348 1107 62-63 1.3577865932227908 1107 64-65 1.246097910083968 1107 66-67 1.0109933419671862 1107 68-69 1.068864995716602 1107 70-71 1.4242617483224898 1107 72-73 0.8694778401751222 1107 74-75 0.8006424798079159 1107 76-77 0.9748512072652531 1107 78-79 0.9342942314324993 1107 80-81 1.158713872655067 1107 82-83 1.170579719331549 1107 84-85 1.1574057639218687 1107 86-87 1.1766471985200901 1107 88-89 1.0626787452775517 1107 90-91 1.0791297098940404 1107 92-93 1.0627757489098464 1107 94-95 0.8320637897405412 1107 96-97 1.0281820794463101 1107 98-99 1.0903553518375162 1107 100-101 1.0313135003744485 1108 1 0.45650446477092643 1108 2 0.35351821513511794 1108 3 0.22312515317314663 1108 4 0.464566643976859 1108 5 0.4513463243728424 1108 6 0.47906337105715835 1108 7 0.5846286845110669 1108 8 0.4877358173940465 1108 9 0.7748690233133004 1108 10-11 0.6369492713437452 1108 12-13 0.7468989060347155 1108 14-15 0.9435358166368175 1108 16-17 0.6861714415583151 1108 18-19 0.6245258012133945 1108 20-21 0.5360294777947843 1108 22-23 0.44100211826713576 1108 24-25 -1.6810731442894564 1108 26-27 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0.8258479032283219 2311 90-91 1.101970277302577 2311 92-93 1.0733840873983844 2311 94-95 1.025799493030764 2311 96-97 1.0792995606357962 2311 98-99 1.5562896193424436 2311 100-101 1.4614520435846785 2312 1 -0.06623499534970634 2312 2 -0.07492560984504948 2312 3 -0.2401497988528405 2312 4 0.28548597229637096 2312 5 -1.2536334445541826 2312 6 -0.006311313732531687 2312 7 0.4126918196404503 2312 8 -2.4576950180406847 2312 9 -4.753947957492663 2312 10-11 -0.415350600577824 2312 12-13 -5.558795154937702 2312 14-15 -1.6882302917733725 2312 16-17 -2.1620721334694935 2312 18-19 -2.1715342361021897 2312 20-21 -3.5079743246719417 2312 22-23 -1.9470542362397651 2312 24-25 -2.423281189741786 2312 26-27 -3.117585450053028 2312 28-29 -5.960095777053169 2312 30-31 -6.3234745758834805 2312 32-33 -1.4060284282483124 2312 34-35 0.9237588176378821 2312 36-37 1.2729927133070902 2312 38-39 0.9614792590027079 2312 40-41 -2.6690482536798115 2312 42-43 -4.924285344598395 2312 44-45 -3.5226527389417974 2312 46-47 0.39161076674140816 2312 48-49 -1.0847256803953442 2312 50-51 -6.2760843754397335 2312 52-53 -1.2440492570935575 2312 54-55 0.7732849692410326 2312 56-57 1.3909508531464851 2312 58-59 1.640560461291109 2312 60-61 1.7202894763097092 2312 62-63 1.5864462411784714 2312 64-65 1.641905137791852 2312 66-67 1.1836088636316262 2312 68-69 1.3812097859071244 2312 70-71 1.7311215092822998 2312 72-73 1.2580924459592921 2312 74-75 1.1634347299115824 2312 76-77 1.4157774994082786 2312 78-79 1.245085214796287 2312 80-81 1.1690004383774522 2312 82-83 0.41408198169251875 2312 84-85 0.850676436159759 2312 86-87 1.243471144460372 2312 88-89 1.1197520977183082 2312 90-91 1.260598171260753 2312 92-93 1.2889964669417502 2312 94-95 1.353211624663377 2312 96-97 1.6238135356048105 2312 98-99 1.6287585305344159 2312 100-101 1.6660134014958317 2313 1 -0.3763010858428437 2313 2 -0.2874234915600127 2313 3 -0.38116827029835676 2313 4 -1.0258680354989167 2313 5 -1.8671142105260472 2313 6 -2.658065253742702 2313 7 -1.5873335997799884 2313 8 -3.4957309441549036 2313 9 -3.2260797995533324 2313 10-11 -1.9949133865463082 2313 12-13 -3.0334308099114367 2313 14-15 -2.792839680012655 2313 16-17 -5.656365473581339 2313 18-19 -3.5847311518791685 2313 20-21 -4.148314944905799 2313 22-23 -3.7584760291562134 2313 24-25 -0.714714845054452 2313 26-27 -3.1290326623899247 2313 28-29 -3.014006131230424 2313 30-31 -2.1416198055055773 2313 32-33 -7.022068082544198 2313 34-35 -2.7053464187627227 2313 36-37 -5.735954922686808 2313 38-39 -2.377408217696157 2313 40-41 -0.9300845187196316 2313 42-43 -3.0530304725428152 2313 44-45 -6.028088258345285 2313 46-47 -3.7278944018740745 2313 48-49 -2.602121037114543 2313 50-51 -1.078918640818486 2313 52-53 -1.395464271497893 2313 54-55 -5.48118884875592 2313 56-57 -6.596402985185996 2313 58-59 -2.7658833617897223 2313 60-61 -9.527177054786716 2313 62-63 -4.423636795594959 2313 64-65 -4.501655275697388 2313 66-67 -6.254838009779661 2313 68-69 -7.230720395418082 2313 70-71 -7.36426486590835 2313 72-73 -3.2819473777993906 2313 74-75 -1.2955086695122482 2313 76-77 -3.30046127368103 2313 78-79 -1.2126083964560443 2313 80-81 -0.8011147963285303 2313 82-83 -0.3891971235438838 2313 84-85 -0.6316528300663045 2313 86-87 -0.659693573384061 2313 88-89 -5.372694097841766 2313 90-91 -1.6099746130130974 2313 92-93 -1.2453960089098004 2313 94-95 -0.5275712934860906 2313 96-97 -0.4427175608195242 2313 98-99 0.05667752731462272 2313 100-101 0.523838346420419 2314 1 -28.747729657271414 2314 2 -14.692185396321918 2314 3 -4.154977794107882 2314 4 -3.818725178356061 2314 5 -1.0790189724308092 2314 6 -0.44854144421888975 2314 7 0.1364759240295399 2314 8 -3.157635706059665 2314 9 -2.2736988471723762 2314 10-11 0.22056280392988725 2314 12-13 0.6808549043742786 2314 14-15 0.7523984152254428 2314 16-17 -0.08493690215276928 2314 18-19 -3.5930644852125084 2314 20-21 -3.562600659191517 2314 22-23 -5.057285552965741 2314 24-25 -0.21352436886397186 2314 26-27 -2.862365995723259 2314 28-29 -1.5901966074209 2314 30-31 -3.292810281696056 2314 32-33 -0.12325855873466907 2314 34-35 -0.08510832352462927 2314 36-37 -2.8216692084010973 2314 38-39 -7.541693931981868 2314 40-41 -5.787227375862489 2314 42-43 -3.5923161868285263 2314 44-45 -2.4673739726310018 2314 46-47 -3.5719420209216963 2314 48-49 -3.422359132352639 2314 50-51 -0.6741567360565739 2314 52-53 -2.039511890545512 2314 54-55 -4.399045991613061 2314 56-57 -0.8999744137574268 2314 58-59 -0.6551690760754312 2314 60-61 0.16925151664185734 2314 62-63 2.026363204405037 2314 64-65 1.6471542481121304 2314 66-67 2.5487334187917625 2314 68-69 2.8883272236295383 2314 70-71 2.7809732293297493 2314 72-73 2.635909765057754 2314 74-75 2.6259199019163297 2314 76-77 -0.1540327022524579 2314 78-79 -0.9590369678846145 2314 80-81 -0.29873384394757707 2314 82-83 0.09056478121802058 2314 84-85 2.1445376461241707 2314 86-87 2.8129254742349836 2314 88-89 2.8546868545391852 2314 90-91 2.804311101272617 2314 92-93 3.2438897053759135 2314 94-95 2.997428706513908 2314 96-97 3.8441872010852336 2314 98-99 4.069772765409862 2314 100-101 4.341695489277562 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 19.0 4 56.0 5 106.0 6 222.0 7 673.0 8 1726.0 9 3074.0 10 4723.0 11 6904.0 12 9270.0 13 11579.0 14 14376.0 15 17206.0 16 19599.0 17 21993.0 18 20025.0 19 17183.0 20 16723.0 21 17348.0 22 19299.0 23 22284.0 24 26349.0 25 32535.0 26 41533.0 27 52494.0 28 66278.0 29 83273.0 30 102071.0 31 124801.0 32 151103.0 33 182201.0 34 223679.0 35 274981.0 36 344417.0 37 429327.0 38 391697.0 39 92598.0 40 89.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.936183768220005 21.687135027825004 15.319485950221226 23.05719525373376 2 14.67800869183306 21.85712241179906 40.705931003344446 22.758937893023433 3 17.89347531841674 26.671962623066808 31.4339628347644 24.000599223752047 4 12.337985892874034 18.19108231876169 39.57168742929822 29.899244359066056 5 11.784772140512706 38.898641050364056 36.576407599090516 12.740179210032723 6 29.265505294113304 39.73607321703667 18.11852301429873 12.879898474551299 7 25.534220004859666 33.586209248900865 23.294239190328216 17.58533155591126 8 24.285863481466297 36.95949003786809 20.77199709264197 17.98264938802364 9 24.796268679546767 16.53537021870942 21.592689267495576 37.07567183424824 10-11 23.322106646027756 27.537107671057825 29.675921709504017 19.464863973410402 12-13 24.224414422999494 24.976571997022315 29.612978181804117 21.186035398174074 14-15 21.414900174729826 26.77533274797025 27.32434799823758 24.485419079062346 16-17 20.788278016287254 29.80107032128275 27.957106271531345 21.453545390898647 18-19 20.722275222157247 28.724138209921236 30.02729779919186 20.526288768729657 20-21 21.532435784415252 27.93665841602437 29.960257667594174 20.570648131966205 22-23 21.315227867877667 27.7708250771234 29.74667164119798 21.167275413800947 24-25 21.17499026175865 27.921714211853132 29.926358671519225 20.97693685486899 26-27 21.178870222994334 28.06229493207482 29.711805934089895 21.04702891084095 28-29 21.014342150886574 28.13423330470811 29.917278733006853 20.934145811398466 30-31 21.412015160884398 27.83000215735088 29.798395824128253 20.959586857636467 32-33 21.28763546164033 28.01732474293145 29.57992034584784 21.115119449580387 34-35 21.42567789958731 27.962584359430952 29.715565598512406 20.89617214246933 36-37 21.340833887171698 27.779442223230266 29.715344663283854 21.16437922631418 38-39 21.193595546807487 28.012616909929577 29.715178884609607 21.078608658653337 40-41 21.401484515549154 27.818191856122677 29.724104318373307 21.056219309954862 42-43 21.319048149025306 27.891010063874365 29.549310241577643 21.240631545522685 44-45 21.405741080824004 28.089837462341354 29.298061310516466 21.20636014631818 46-47 21.492366837824633 27.967213933620915 29.094802189255425 21.445617039299027 48-49 21.450882203879537 28.05009853067429 29.121872402864724 21.37714686258145 50-51 21.46998040363997 28.210206155959995 28.915928518334987 21.40388492206505 52-53 21.451906953584572 28.30711836620732 28.819063188623105 21.421911491585007 54-55 21.54570962677013 28.147502845380668 28.830464559950514 21.47632296789869 56-57 21.664336738936914 28.264095125050392 28.63704766186434 21.434520474148353 58-59 21.443566618807647 28.339116151839338 28.85107756642681 21.36623966292621 60-61 21.535374833470154 28.327999044909475 28.707925334848728 21.428700786771646 62-63 21.520056320653566 28.25543118390473 28.630837738073094 21.593674757368614 64-65 21.2578162269452 28.164528972593335 28.7818264909715 21.79582830948997 66-67 21.438687966313672 28.23177916101738 28.559281190541792 21.770251682127157 68-69 21.498134861078093 28.164406510263184 28.577645198814526 21.75981342984419 70-71 21.52326625747368 27.914673626745383 28.593661095649743 21.968399020131198 72-73 21.558297379423262 28.04772466168042 28.624352820827326 21.769625138068992 74-75 21.42932118241481 28.292660799028617 28.427076467130753 21.85094155142582 76-77 21.30218203057227 28.242567477044073 28.40822499603526 22.047025496348397 78-79 21.381160334043763 28.33351911637133 28.212537436454287 22.07278311313062 80-81 21.356379443604805 28.332312401056893 28.20079850735247 22.110509647985833 82-83 21.4317688560815 28.253933815410626 28.375355685553576 21.938941642954298 84-85 21.524074325431513 28.2203361818513 28.117182604512664 22.13840688820452 86-87 21.21143923331474 28.309646211399674 28.30467043009212 22.17424412519347 88-89 21.440551504997256 28.191575123970996 28.14538545515989 22.22248791587186 90-91 21.340904000059076 28.17730818420393 28.230371304702224 22.251416511034773 92-93 21.40793823298614 28.201736564290737 28.15938005115485 22.23094515156827 94-95 21.29681594601019 28.143087619505756 28.13496467397802 22.42513176050603 96-97 21.174700177385485 28.10363503986646 28.239175204079466 22.48248957866859 98-99 21.12577223809223 28.26615152772022 28.02175708352681 22.586319150660735 100-101 21.13227133481367 28.19699784771848 27.99400715926795 22.6767236581999 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 298.0 1 217.5 2 147.5 3 175.5 4 168.0 5 187.5 6 307.5 7 612.5 8 1069.0 9 1405.5 10 1451.0 11 1376.5 12 1510.5 13 1752.5 14 1985.0 15 2233.0 16 2501.0 17 2800.0 18 3169.5 19 3685.0 20 4290.0 21 5117.5 22 6368.0 23 8309.0 24 10940.0 25 14431.5 26 19442.5 27 25755.0 28 32372.5 29 39372.0 30 46482.0 31 53901.0 32 62860.0 33 71138.5 34 79684.5 35 87724.0 36 95051.0 37 101951.5 38 103480.5 39 102823.0 40 104433.5 41 105370.0 42 103997.0 43 103212.5 44 103404.0 45 103690.0 46 102088.0 47 99843.0 48 98631.5 49 96002.0 50 92074.0 51 87930.5 52 83130.5 53 77785.0 54 72973.0 55 66979.5 56 60064.0 57 52901.5 58 46571.5 59 41009.0 60 34099.5 61 27080.5 62 20744.0 63 15614.5 64 11624.5 65 8461.0 66 6242.5 67 4724.5 68 3723.0 69 2923.0 70 2183.5 71 1591.0 72 1128.5 73 827.5 74 645.5 75 468.5 76 335.5 77 229.0 78 161.0 79 119.5 80 86.5 81 73.0 82 56.0 83 48.5 84 44.5 85 29.0 86 20.0 87 20.0 88 13.5 89 5.5 90 4.0 91 3.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10018218459051016 2 0.0 3 0.004922957473735143 4 2.8131185564200816E-4 5 1.7581990977625508E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.022733514334069786 26-27 0.008755831506857504 28-29 0.0060833688782584265 30-31 0.0018812730346059294 32-33 8.790995488812755E-4 34-35 0.0012307393684337857 36-37 0.0010197554767022797 38-39 5.802057022616418E-4 40-41 0.0022153308631808143 42-43 0.0011779933955009092 44-45 5.802057022616418E-4 46-47 0.03607824548608755 48-49 0.019058878219746053 50-51 0.08703085533924627 52-53 0.0019516009985164314 54-55 0.03704525498985695 56-57 8.96681539858901E-4 58-59 0.001758199097762551 60-61 0.0038504560240999867 62-63 0.003586726159435604 64-65 0.010390956667776676 66-67 0.006065786887280801 68-69 0.0022153308631808143 70-71 0.0060833688782584265 72-73 0.004641645618093135 74-75 0.0010021734857246542 76-77 0.0 78-79 0.0 80-81 4.571317654182633E-4 82-83 5.274597293287653E-4 84-85 3.516398195525102E-5 86-87 0.0015647971970086704 88-89 0.0038504560240999867 90-91 0.0012659033503890367 92-93 0.0035339801865027273 94-95 8.087715849707734E-4 96-97 4.395497744406378E-4 98-99 0.0019516009985164314 100-101 0.0032526683308607193 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2843819.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.20224213504707 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2624104639262 51.53026710947526 2 12.21759574571186 15.687940807486275 3 3.5349547862194894 6.808560693639216 4 1.4538043619733552 3.7334999865760388 5 0.733832111357944 2.3556833449937775 6 0.40544230583244134 1.5618183054507726 7 0.2564124632478786 1.1523578536318908 8 0.18272923160739676 0.9385301098247346 9 0.13851022870506446 0.8003400517352965 >10 0.7254366739740273 8.614176281365674 >50 0.0604356324173388 2.6385278384398223 >100 0.02733289516339023 3.0679583828135746 >500 7.721699045751698E-4 0.3368623912333149 >1k 2.2061997269406117E-4 0.28087800766048737 >5k 1.1030998634703059E-4 0.4925988356738578 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7720 0.2714659406945379 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6191 0.21770021228495906 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4031 0.14174601126161684 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.516398195525102E-5 2 0.0 0.0 0.0 0.0 3.516398195525102E-5 3 0.0 0.0 0.0 3.516398195525102E-5 3.516398195525102E-5 4 0.0 0.0 0.0 7.032796391050204E-5 3.516398195525102E-5 5 0.0 0.0 0.0 7.032796391050204E-5 3.516398195525102E-5 6 0.0 0.0 0.0 7.032796391050204E-5 3.516398195525102E-5 7 0.0 0.0 0.0 7.032796391050204E-5 3.516398195525102E-5 8 0.0 0.0 0.0 7.032796391050204E-5 3.516398195525102E-5 9 0.0 0.0 0.0 1.4065592782100408E-4 3.516398195525102E-5 10-11 3.516398195525102E-5 0.0 0.0 1.582379187986296E-4 3.516398195525102E-5 12-13 3.516398195525102E-5 0.0 0.0 1.758199097762551E-4 3.516398195525102E-5 14-15 3.516398195525102E-5 0.0 0.0 1.934019007538806E-4 3.516398195525102E-5 16-17 3.516398195525102E-5 0.0 0.0 2.109838917315061E-4 3.516398195525102E-5 18-19 3.516398195525102E-5 0.0 0.0 2.6372986466438266E-4 8.790995488812755E-5 20-21 3.516398195525102E-5 0.0 0.0 2.9889384661963366E-4 1.4065592782100408E-4 22-23 3.516398195525102E-5 0.0 0.0 3.1647583759725916E-4 1.4065592782100408E-4 24-25 7.032796391050204E-5 0.0 0.0 4.571317654182633E-4 1.4065592782100408E-4 26-27 1.0549194586575305E-4 0.0 0.0 9.67009503769403E-4 1.4065592782100408E-4 28-29 1.0549194586575305E-4 0.0 0.0 0.0026548806376214523 1.4065592782100408E-4 30-31 1.0549194586575305E-4 0.0 0.0 0.008316281732416867 1.582379187986296E-4 32-33 1.0549194586575305E-4 0.0 0.0 0.022100562658875264 1.758199097762551E-4 34-35 1.0549194586575305E-4 0.0 0.0 0.04573075853280395 1.758199097762551E-4 36-37 1.0549194586575305E-4 0.0 0.0 0.08251228365799651 1.758199097762551E-4 38-39 1.0549194586575305E-4 0.0 0.0 0.13186493233219132 1.758199097762551E-4 40-41 1.758199097762551E-4 0.0 0.0 0.18353840381543268 1.934019007538806E-4 42-43 1.758199097762551E-4 0.0 0.0 0.23966011901601333 2.109838917315061E-4 44-45 1.758199097762551E-4 0.0 0.0 0.30803648192799893 2.109838917315061E-4 46-47 1.758199097762551E-4 0.0 0.0 0.3920080708371384 2.109838917315061E-4 48-49 1.758199097762551E-4 0.0 0.0 0.47910925414029515 2.109838917315061E-4 50-51 1.758199097762551E-4 0.0 0.0 0.5675818327397066 2.109838917315061E-4 52-53 2.109838917315061E-4 0.0 0.0 0.656581871068447 2.109838917315061E-4 54-55 2.109838917315061E-4 0.0 0.0 0.7552695864258591 2.109838917315061E-4 56-57 2.109838917315061E-4 3.516398195525102E-5 0.0 0.8698514216270445 2.2856588270913164E-4 58-59 2.109838917315061E-4 3.516398195525102E-5 0.0 0.9979362258990463 2.4614787368675716E-4 60-61 2.4614787368675716E-4 7.032796391050204E-5 0.0 1.1265484899003768 2.4614787368675716E-4 62-63 2.8131185564200816E-4 7.032796391050204E-5 0.0 1.254158580415983 2.4614787368675716E-4 64-65 2.8131185564200816E-4 7.032796391050204E-5 0.0 1.3915618399061263 2.4614787368675716E-4 66-67 2.8131185564200816E-4 7.032796391050204E-5 0.0 1.5495008648581363 2.4614787368675716E-4 68-69 3.1647583759725916E-4 7.032796391050204E-5 0.0 1.7288020088479612 2.6372986466438266E-4 70-71 3.1647583759725916E-4 7.032796391050204E-5 0.0 1.9202523086033254 2.8131185564200816E-4 72-73 3.1647583759725916E-4 7.032796391050204E-5 0.0 2.121021063576831 2.9889384661963366E-4 74-75 3.1647583759725916E-4 7.032796391050204E-5 0.0 2.321367850766874 3.1647583759725916E-4 76-77 3.1647583759725916E-4 7.032796391050204E-5 0.0 2.541441631833812 3.1647583759725916E-4 78-79 3.1647583759725916E-4 7.032796391050204E-5 0.0 2.789031228780735 3.1647583759725916E-4 80-81 3.1647583759725916E-4 7.032796391050204E-5 0.0 3.054659948470701 3.1647583759725916E-4 82-83 3.1647583759725916E-4 7.032796391050204E-5 0.0 3.329853271252495 3.1647583759725916E-4 84-85 3.1647583759725916E-4 7.032796391050204E-5 0.0 3.603464214846303 3.516398195525102E-4 86-87 3.1647583759725916E-4 7.032796391050204E-5 0.0 3.8966614963891866 3.516398195525102E-4 88-89 3.1647583759725916E-4 7.032796391050204E-5 0.0 4.210623109276645 3.516398195525102E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3985 0.0 53.438652 1 GTATCAA 7440 0.0 41.592392 1 ATCAACG 9065 0.0 33.737972 3 TCAACGC 9270 0.0 33.14382 4 CAACGCA 9400 0.0 32.53332 5 TATCAAC 9595 0.0 32.022865 2 AACGCAG 9760 0.0 31.479284 6 ACGCAGA 11185 0.0 26.95927 7 CGCAGAG 11155 0.0 26.733784 8 GTGGTAT 1730 0.0 24.728754 1 GCAGAGT 12560 0.0 22.949299 9 TGGTATC 1870 0.0 21.840258 2 GTACATG 7625 0.0 21.38258 1 AGAGTAC 11255 0.0 21.053555 10-11 GAGTACT 8135 0.0 20.66408 12-13 TACATGG 7760 0.0 20.501425 2 CTAACGC 240 1.4182115E-7 19.787445 3 ACATGGG 7610 0.0 19.71984 3 CAGAGTA 12465 0.0 19.54319 10-11 AGTACTT 8745 0.0 19.222675 12-13 >>END_MODULE