FastQCFastQC Report
Sat 18 Jun 2016
SRR1931124_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931124_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691570
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40780.15151008519191403No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.11056743833524672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.047.6627041
GTATCAA50400.039.151511
ATCAACG56050.034.2343753
CAACGCA56750.034.187625
TCAACGC56750.033.9849784
AACGCAG59950.032.7416766
TATCAAC63650.030.702392
ACGCAGA68750.028.0065637
CGCAGAG70200.027.2249098
GCAGAGT78000.022.8567339
GTACATG59750.021.936811
TACATGG60050.019.9226672
AGAGTAC70250.019.3548210-11
GAGTACT46250.019.32212412-13
CAGAGTA80100.018.96303210-11
ACATGGG62800.018.8787273
TATACCG3557.8216544E-1118.753155
GTGGTAT16000.017.8735141
CCTATAC9100.017.7897783
TGTACCG3751.6916601E-1017.7529815
Produced by FastQC (version 0.11.5)