##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931124_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2691570 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.92625939507425 31.0 28.0 31.0 24.0 34.0 2 28.68307196171751 31.0 28.0 33.0 22.0 34.0 3 28.678185965811775 31.0 28.0 33.0 20.0 34.0 4 31.885507714828147 35.0 32.0 37.0 19.0 37.0 5 31.976784181722934 35.0 32.0 37.0 20.0 37.0 6 32.261194767366256 35.0 32.0 37.0 23.0 37.0 7 31.974084642049064 35.0 32.0 37.0 19.0 37.0 8 32.08974761941915 35.0 32.0 37.0 22.0 37.0 9 33.12843619151648 37.0 32.0 39.0 19.0 39.0 10-11 33.10517913336826 37.0 32.0 39.0 20.5 39.0 12-13 33.21522661494964 37.0 32.0 39.0 22.5 39.0 14-15 33.92670876105767 38.0 32.0 40.0 20.0 41.0 16-17 33.8742899125789 38.0 32.0 40.0 19.0 41.0 18-19 33.905736800454754 38.0 32.0 40.0 19.5 41.0 20-21 34.08747273895905 38.0 32.0 40.0 23.0 41.0 22-23 34.00973038040995 38.0 32.0 40.0 22.5 41.0 24-25 33.88000572156771 38.0 32.0 40.0 21.0 41.0 26-27 33.525795353641186 38.0 32.0 40.0 18.0 41.0 28-29 33.48960569481753 37.5 32.0 40.0 18.5 41.0 30-31 33.274637107710376 37.0 31.5 40.0 18.0 41.0 32-33 33.07801896291012 37.0 31.0 40.0 17.5 41.0 34-35 33.06113104990767 37.0 31.0 40.0 18.0 41.0 36-37 32.732498504590254 37.0 30.0 40.0 16.0 41.0 38-39 32.55777984596351 37.0 30.0 40.0 15.5 41.0 40-41 32.41133966421085 37.0 30.0 40.0 15.0 41.0 42-43 32.62308355346508 37.0 30.0 40.0 15.0 41.0 44-45 32.7307820342774 37.0 30.5 40.0 15.0 41.0 46-47 32.53559632482157 37.0 30.0 40.0 13.0 41.0 48-49 32.31251462900835 37.0 30.0 40.0 9.0 41.0 50-51 31.789790531176973 36.5 30.0 39.5 8.5 40.5 52-53 31.73974966283619 36.0 30.0 39.5 8.0 40.5 54-55 31.768598253064198 36.0 30.0 40.0 7.5 41.0 56-57 31.53748184145313 36.0 30.0 40.0 6.5 41.0 58-59 31.39576362494752 36.0 29.0 40.0 2.0 41.0 60-61 31.23856671013572 36.0 29.0 39.5 2.0 41.0 62-63 31.081504846613687 35.0 29.0 39.0 2.0 41.0 64-65 30.745035239655664 35.0 29.0 39.0 2.0 40.5 66-67 30.54598022715367 35.0 29.0 39.0 2.0 40.0 68-69 30.32862511471 35.0 29.0 39.0 2.0 40.0 70-71 30.02222996243828 34.0 28.5 38.0 2.0 40.0 72-73 29.69147504987795 34.0 28.5 37.5 2.0 40.0 74-75 29.27038754332973 34.0 28.0 37.0 2.0 39.5 76-77 28.95501231623179 34.0 28.0 36.5 2.0 39.0 78-79 28.52121772794317 34.0 27.5 36.0 2.0 39.0 80-81 28.094456766868408 33.0 26.5 36.0 2.0 37.5 82-83 27.745329677474487 33.0 26.0 35.0 2.0 37.0 84-85 27.39650798604532 33.0 26.0 35.0 2.0 37.0 86-87 27.05192601344197 33.0 26.0 35.0 2.0 36.0 88-89 26.69627336461619 33.0 25.5 35.0 2.0 36.0 90-91 26.31350178520343 32.0 25.0 35.0 2.0 36.0 92-93 25.952400643490602 32.0 24.0 35.0 2.0 35.0 94-95 25.64234108717217 32.0 23.5 35.0 2.0 35.0 96-97 25.249597261078108 32.0 20.0 35.0 2.0 35.0 98-99 24.86190996332995 32.0 18.5 34.5 2.0 35.0 100-101 24.26206061889529 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.185657417332628 1101 2 5.81408195819796 1101 3 5.368171582280947 1101 4 6.692436456871828 1101 5 6.273069535405483 1101 6 5.723897663421518 1101 7 6.23252145034273 1101 8 5.6718188342450695 1101 9 6.460951645407427 1101 10-11 6.447058428592836 1101 12-13 6.271300322768688 1101 14-15 6.569719811271565 1101 16-17 6.3649170047684045 1101 18-19 6.57783344290598 1101 20-21 5.880222375038727 1101 22-23 5.834481605284147 1101 24-25 5.86040752221059 1101 26-27 5.96201700805403 1101 28-29 5.831840354771426 1101 30-31 5.998688475519245 1101 32-33 5.788130409012865 1101 34-35 5.615500506329489 1101 36-37 5.697935184394957 1101 38-39 5.715250099614824 1101 40-41 5.895706969344513 1101 42-43 5.609749724748063 1101 44-45 5.682907865799535 1101 46-47 5.571489554927968 1101 48-49 5.511766359221799 1101 50-51 5.451510112656472 1101 52-53 5.307327796765737 1101 54-55 5.415463742692868 1101 56-57 5.375373386597918 1101 58-59 5.246904590758607 1101 60-61 5.170685442748368 1101 62-63 5.160164077528108 1101 64-65 5.142282931342244 1101 66-67 5.123184012401765 1101 68-69 5.119132115083005 1101 70-71 4.831882305624045 1101 72-73 4.6429601375006335 1101 74-75 4.813415293304459 1101 76-77 4.685670381193688 1101 78-79 4.617489993659696 1101 80-81 4.562431277233287 1101 82-83 4.4274401208113865 1101 84-85 4.219663851389598 1101 86-87 4.0576383915898475 1101 88-89 4.217645694520883 1101 90-91 4.210478290520964 1101 92-93 4.009151051272031 1101 94-95 3.960209276553165 1101 96-97 4.034687377016457 1101 98-99 3.8041531123046077 1101 100-101 3.744069263192607 1102 1 5.303421648006289 1102 2 5.907032893886143 1102 3 5.527086224352189 1102 4 6.8429044980019675 1102 5 6.5254753488349735 1102 6 5.942075833708479 1102 7 6.462958228391919 1102 8 5.958171584795981 1102 9 6.712778118332373 1102 10-11 6.758774691708869 1102 12-13 6.678733831341916 1102 14-15 6.9820425067907586 1102 16-17 6.892393780697358 1102 18-19 7.001435544676074 1102 20-21 6.2687894898909775 1102 22-23 6.320580875959486 1102 24-25 6.129954114863022 1102 26-27 6.323132464146749 1102 28-29 6.2730470156368945 1102 30-31 6.436892335473878 1102 32-33 6.056364521298903 1102 34-35 6.2532091336527955 1102 36-37 6.38052613648032 1102 38-39 6.167557250204471 1102 40-41 6.267376356893518 1102 42-43 6.282942568225572 1102 44-45 6.204808197060359 1102 46-47 6.217664292304146 1102 48-49 5.82469647450873 1102 50-51 5.836132121342338 1102 52-53 5.622978144807423 1102 54-55 5.821174624845103 1102 56-57 5.850092024499009 1102 58-59 5.754451758854049 1102 60-61 5.703438101768857 1102 62-63 5.5271643781546445 1102 64-65 5.690224982048271 1102 66-67 5.516919352960702 1102 68-69 5.479288355136276 1102 70-71 5.285635295758109 1102 72-73 5.078654727271001 1102 74-75 5.163117615871791 1102 76-77 5.05680163338765 1102 78-79 4.975734882077536 1102 80-81 5.011652610336583 1102 82-83 4.899074630139253 1102 84-85 4.86492843781765 1102 86-87 4.944288265263474 1102 88-89 4.9204389272971945 1102 90-91 4.923536455735988 1102 92-93 4.839059355415316 1102 94-95 4.707515871533701 1102 96-97 4.680658427140191 1102 98-99 4.455199671135471 1102 100-101 4.553180832533272 1103 1 5.1708333197091925 1103 2 5.638897889400241 1103 3 5.258769949385904 1103 4 6.654276372963306 1103 5 6.2947097082300445 1103 6 5.686969223713895 1103 7 6.234850339819722 1103 8 5.631888602335575 1103 9 6.146956968175651 1103 10-11 6.3192851372656555 1103 12-13 6.424459832627747 1103 14-15 6.612759704790246 1103 16-17 6.490982417866714 1103 18-19 6.357312244913405 1103 20-21 5.785369360889874 1103 22-23 6.018066190661514 1103 24-25 5.809758262539798 1103 26-27 6.016723727157032 1103 28-29 5.702945104852343 1103 30-31 6.054850037037568 1103 32-33 5.740919478592197 1103 34-35 5.839872570812425 1103 36-37 5.760893037488891 1103 38-39 5.6514948042517865 1103 40-41 5.796846376445476 1103 42-43 5.726592790003544 1103 44-45 5.447133233077679 1103 46-47 5.628024994536343 1103 48-49 5.429338240268368 1103 50-51 5.394813095962341 1103 52-53 5.216691548882753 1103 54-55 5.46452369615918 1103 56-57 5.528416464955992 1103 58-59 5.529778133175672 1103 60-61 5.47189503307165 1103 62-63 5.5304027588618965 1103 64-65 5.534588852354336 1103 66-67 5.466113895989725 1103 68-69 5.4706503623964835 1103 70-71 5.28228319971419 1103 72-73 4.875393607762604 1103 74-75 4.877639817346104 1103 76-77 5.071492008134186 1103 78-79 4.995920068921794 1103 80-81 4.93970352392018 1103 82-83 4.8226684400659146 1103 84-85 4.786355555693799 1103 86-87 4.7771051547746595 1103 88-89 4.719227606189911 1103 90-91 4.699203634772264 1103 92-93 4.664741871410335 1103 94-95 4.6392401253874525 1103 96-97 4.567409363889208 1103 98-99 4.44297332066186 1103 100-101 4.5660639845321285 1104 1 4.56734181064493 1104 2 5.344131813829211 1104 3 4.980966718331899 1104 4 6.506703317650317 1104 5 6.243670471122698 1104 6 5.703970248145261 1104 7 6.300510216005918 1104 8 5.5897513335729165 1104 9 6.3751229084158965 1104 10-11 6.336821562542017 1104 12-13 6.2787172080925515 1104 14-15 6.333289284143358 1104 16-17 6.234312147419363 1104 18-19 6.555113623674533 1104 20-21 5.81575559545567 1104 22-23 5.923567752509825 1104 24-25 5.657338941235036 1104 26-27 5.901514197551219 1104 28-29 5.687695379231105 1104 30-31 6.018405355701248 1104 32-33 5.81816248555657 1104 34-35 5.767823670280556 1104 36-37 5.827941692308443 1104 38-39 5.595532311292391 1104 40-41 5.945873670674015 1104 42-43 5.859600649017587 1104 44-45 5.741944796929488 1104 46-47 5.856190304744995 1104 48-49 5.555064257170859 1104 50-51 5.55258390559073 1104 52-53 5.3541732622158555 1104 54-55 5.534122907863658 1104 56-57 5.482327197040096 1104 58-59 5.38495869392899 1104 60-61 5.304482093986881 1104 62-63 5.2074346422405355 1104 64-65 5.380894914140271 1104 66-67 5.297136651470684 1104 68-69 5.2228873525591695 1104 70-71 5.157610154607706 1104 72-73 5.026317108066909 1104 74-75 5.012276434026063 1104 76-77 4.980846339160344 1104 78-79 4.948701028359103 1104 80-81 4.998625905288382 1104 82-83 4.745234659536155 1104 84-85 4.741037320437485 1104 86-87 4.6541939300058495 1104 88-89 4.61927221242647 1104 90-91 4.7294893792994905 1104 92-93 4.632937372732769 1104 94-95 4.4398550992222425 1104 96-97 4.343442017166446 1104 98-99 4.247600776682507 1104 100-101 4.411757907625436 1105 1 4.355033863961982 1105 2 5.025653995351394 1105 3 4.697821622686803 1105 4 6.067289143861142 1105 5 3.593360133312359 1105 6 4.8145662493662655 1105 7 5.578224399970097 1105 8 0.8707596770812636 1105 9 0.2714586328766231 1105 10-11 1.845260461605914 1105 12-13 5.2461857927486335 1105 14-15 3.1798596697762456 1105 16-17 5.401144158001369 1105 18-19 6.110246280369697 1105 20-21 3.328630163017742 1105 22-23 5.224280639160206 1105 24-25 5.429688987022583 1105 26-27 5.534940377852404 1105 28-29 2.8921431305538583 1105 30-31 5.2631366082449915 1105 32-33 5.3927262319953115 1105 34-35 5.398146472478352 1105 36-37 5.511855001221747 1105 38-39 5.455927992000564 1105 40-41 5.476843857894192 1105 42-43 2.8466942845487253 1105 44-45 4.950548403970593 1105 46-47 5.283839623019315 1105 48-49 5.13827994194904 1105 50-51 5.184264435708762 1105 52-53 5.047301314718911 1105 54-55 5.197940571681325 1105 56-57 5.151203748729149 1105 58-59 5.089848422256221 1105 60-61 4.933504402696695 1105 62-63 4.641974350217744 1105 64-65 4.816003786749146 1105 66-67 4.851293143127176 1105 68-69 5.008747904044721 1105 70-71 4.721210327582881 1105 72-73 4.683845343720144 1105 74-75 4.721996112495745 1105 76-77 4.758245733747238 1105 78-79 4.626331268489341 1105 80-81 4.53502700418948 1105 82-83 4.373458865885365 1105 84-85 4.468620501145665 1105 86-87 4.452286751536171 1105 88-89 4.345672548201804 1105 90-91 4.305582556955169 1105 92-93 4.35200852149142 1105 94-95 4.294075820630468 1105 96-97 4.379709569058999 1105 98-99 4.335700466344694 1105 100-101 4.430479926347452 1106 1 4.043257788589305 1106 2 4.35684624236514 1106 3 4.306020936895244 1106 4 5.982001364956478 1106 5 6.0141218287951475 1106 6 5.063155124571516 1106 7 5.772378067184114 1106 8 5.140413803099488 1106 9 5.726390677533566 1106 10-11 5.781846374706792 1106 12-13 5.9442918670749165 1106 14-15 5.675279425322142 1106 16-17 5.628043969650168 1106 18-19 6.000976171464064 1106 20-21 5.305425586646962 1106 22-23 5.273401113499375 1106 24-25 5.261732688123711 1106 26-27 5.405127461306474 1106 28-29 5.334354425991567 1106 30-31 5.490241422871996 1106 32-33 5.352311718488764 1106 34-35 5.003889272471916 1106 36-37 5.064291493059862 1106 38-39 5.1156348704416 1106 40-41 5.266469047050318 1106 42-43 2.191902446836629 1106 44-45 4.371893917750825 1106 46-47 4.618345623695443 1106 48-49 4.439502765848719 1106 50-51 4.670784295089291 1106 52-53 4.461249868278262 1106 54-55 4.786868096714326 1106 56-57 4.694589673501358 1106 58-59 4.585097635447244 1106 60-61 4.4359888375625935 1106 62-63 4.483934661641165 1106 64-65 4.538751219055403 1106 66-67 4.720326676080585 1106 68-69 4.795341061422615 1106 70-71 4.58669466787072 1106 72-73 4.335322673157666 1106 74-75 4.408573473325131 1106 76-77 4.432437644707022 1106 78-79 4.468146442834943 1106 80-81 4.442314344412928 1106 82-83 4.452807343782272 1106 84-85 4.511775766125222 1106 86-87 4.6190965429814845 1106 88-89 4.622664020077913 1106 90-91 4.5730550991329615 1106 92-93 4.561309016114556 1106 94-95 4.479279032662404 1106 96-97 4.566275359168145 1106 98-99 4.466336664789591 1106 100-101 4.3749082723985175 1107 1 3.1082270043174596 1107 2 -0.1278980425777938 1107 3 1.0614596622109396 1107 4 2.787949702475501 1107 5 2.89830051391516 1107 6 4.044849755576731 1107 7 5.141014235450058 1107 8 4.414857684487945 1107 9 5.018132011163473 1107 10-11 2.5979770291345083 1107 12-13 4.90680560028968 1107 14-15 3.458590507861654 1107 16-17 1.9664827705053298 1107 18-19 2.6716209915583633 1107 20-21 2.3742832623797625 1107 22-23 4.291567618617059 1107 24-25 4.175479800910647 1107 26-27 3.481560295319273 1107 28-29 4.214092142723533 1107 30-31 3.8020342195090464 1107 32-33 4.057369341762737 1107 34-35 4.664399278659332 1107 36-37 4.759663312433361 1107 38-39 4.587558618700658 1107 40-41 3.490053732920142 1107 42-43 3.969986764393383 1107 44-45 4.201234672255154 1107 46-47 4.577768132998667 1107 48-49 2.271337214668865 1107 50-51 3.8769535661613546 1107 52-53 3.980879659741248 1107 54-55 4.5581859315825675 1107 56-57 3.2817822282570006 1107 58-59 2.3719898626532085 1107 60-61 3.404925480734473 1107 62-63 4.182729605491453 1107 64-65 4.3840465062457525 1107 66-67 4.481844738621991 1107 68-69 4.389266555930803 1107 70-71 4.125273930254966 1107 72-73 4.170132438564966 1107 74-75 4.259511884461787 1107 76-77 4.23221012665627 1107 78-79 4.095474884871432 1107 80-81 4.127019897928761 1107 82-83 3.9466952391798067 1107 84-85 3.8951175065204247 1107 86-87 3.9725398164866306 1107 88-89 3.8532685250532026 1107 90-91 4.005551200784577 1107 92-93 3.8911329827866084 1107 94-95 4.007823256832523 1107 96-97 4.048659927649325 1107 98-99 4.0520064090303265 1107 100-101 4.188380930291125 1108 1 -1.8968747297921382 1108 2 -1.1020075419747073 1108 3 -1.8355826805821636 1108 4 -3.747015004815932 1108 5 -8.110015518398566 1108 6 -6.652813526772022 1108 7 -5.303003261303644 1108 8 -5.790578313777651 1108 9 -7.485061029815203 1108 10-11 -3.268851139112087 1108 12-13 -1.8520155197009558 1108 14-15 -6.439338809806554 1108 16-17 -5.645058291482798 1108 18-19 -3.823241274192153 1108 20-21 -4.461198212633967 1108 22-23 -3.4634002864010895 1108 24-25 -7.917683189665542 1108 26-27 -8.907974704016727 1108 28-29 -6.028174912942742 1108 30-31 -5.669325440631347 1108 32-33 -2.505923247031326 1108 34-35 -0.770476068284875 1108 36-37 -4.494287160059898 1108 38-39 -5.248189771635939 1108 40-41 -2.9792745478983207 1108 42-43 -3.8361772252724045 1108 44-45 -1.6128313481156695 1108 46-47 -2.225900704114526 1108 48-49 -1.3557159075946963 1108 50-51 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-6.939570464699983 2313 48-49 -4.653014700908102 2313 50-51 -5.651007337474198 2313 52-53 -3.6192217638107493 2313 54-55 -4.5284587599811665 2313 56-57 -3.2840706351472093 2313 58-59 -3.5199638688883077 2313 60-61 -4.440901664554769 2313 62-63 -6.959483722046251 2313 64-65 -8.099879671897469 2313 66-67 -9.09508321443339 2313 68-69 -9.47186769745904 2313 70-71 -7.2136920324839515 2313 72-73 -5.605824142495397 2313 74-75 -6.78293809803057 2313 76-77 -5.95079901353764 2313 78-79 -6.226164554401219 2313 80-81 -7.106942502911604 2313 82-83 -6.076402767364428 2313 84-85 -6.270610388578643 2313 86-87 -6.205144587227405 2313 88-89 -7.037015943861686 2313 90-91 -6.925770565434137 2313 92-93 -6.238354103821864 2313 94-95 -6.166848625902535 2313 96-97 -6.171290326513267 2313 98-99 -6.6387604254681705 2313 100-101 -7.474699689358477 2314 1 -13.664444921141804 2314 2 -14.739303269605871 2314 3 -13.911381674016493 2314 4 -25.000419189472193 2314 5 -19.058120330668103 2314 6 -16.067702523527508 2314 7 -16.431276515780816 2314 8 -13.38908464526306 2314 9 -16.564641031348042 2314 10-11 -14.269609043888591 2314 12-13 -17.44157176219642 2314 14-15 -17.420271969417893 2314 16-17 -18.077144914037703 2314 18-19 -16.88800806944716 2314 20-21 -17.231761702061622 2314 22-23 -17.405288576346504 2314 24-25 -16.661647627751535 2314 26-27 -16.731676685639663 2314 28-29 -17.59547515393943 2314 30-31 -19.15212781483193 2314 32-33 -15.613664946270866 2314 34-35 -17.693714791257904 2314 36-37 -16.629628515261768 2314 38-39 -16.014052548292476 2314 40-41 -19.725270000469664 2314 42-43 -19.563476274059333 2314 44-45 -15.622931067946375 2314 46-47 -14.85117640262171 2314 48-49 -14.191476239369642 2314 50-51 -13.148983045976223 2314 52-53 -13.334471426428834 2314 54-55 -13.971778598037847 2314 56-57 -12.767876303163403 2314 58-59 -11.896482087511789 2314 60-61 -11.747919208414416 2314 62-63 -14.667310982505091 2314 64-65 -12.517558484313124 2314 66-67 -12.301560947226914 2314 68-69 -16.10749522782341 2314 70-71 -16.235959238961684 2314 72-73 -14.88180255005275 2314 74-75 -13.450279528529897 2314 76-77 -13.94202708371308 2314 78-79 -13.74370841405034 2314 80-81 -13.668346011683536 2314 82-83 -14.763312349010853 2314 84-85 -14.952796623396459 2314 86-87 -14.761149985338065 2314 88-89 -14.062656969502713 2314 90-91 -13.321182171372058 2314 92-93 -12.925263685468288 2314 94-95 -12.801801392434248 2314 96-97 -12.808267384542956 2314 98-99 -12.878976349894621 2314 100-101 -12.361339365471839 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79563.0 3 14355.0 4 9729.0 5 8005.0 6 8021.0 7 9050.0 8 10535.0 9 12489.0 10 13560.0 11 14896.0 12 16406.0 13 17528.0 14 19247.0 15 21231.0 16 22544.0 17 23448.0 18 22781.0 19 21591.0 20 21727.0 21 23440.0 22 27161.0 23 32906.0 24 40173.0 25 49896.0 26 59199.0 27 68672.0 28 77410.0 29 86606.0 30 98842.0 31 110401.0 32 126985.0 33 151999.0 34 183360.0 35 221922.0 36 270307.0 37 325890.0 38 295741.0 39 73884.0 40 70.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.78633190504383 20.253316043708704 14.43007235105817 23.530279700189286 2 14.4899788336835 20.7479385257841 41.24217145795329 23.519911182579108 3 17.744253353990423 25.795836630665374 30.901035455143283 25.558874560200923 4 11.452128303785205 17.860237192446363 40.45321830835018 30.234416195418255 5 12.48359435299479 37.82709369565193 36.14763946708129 13.541672484271988 6 29.4876467271897 38.2854023274045 19.0121695616667 13.21478138373911 7 25.923152940948935 32.64737289015 23.4116220139632 18.01785215493787 8 24.422456851870518 35.83235357457282 21.54789858018357 18.197290993373098 9 25.09184137635791 16.471974478939437 21.701828049734377 36.73435609496828 10-11 23.29485508306991 26.842052699872067 30.018726135521874 19.84436608153615 12-13 24.37058977424929 24.484151749911334 29.476952482602858 21.668305993236523 14-15 21.683749719374426 26.128802999638605 27.192511096218812 24.994936184768154 16-17 21.223207703117804 29.153638306688894 27.840554448315448 21.782599541877854 18-19 21.05530120719473 28.426141861284393 29.558805312088353 20.95975161943252 20-21 21.979103577815433 27.619728034480378 29.425957390072682 20.975210997631503 22-23 21.689643848414196 27.529253462569365 29.145980756664525 21.635121932351915 24-25 21.498222582040324 27.679152164753383 29.211387226465625 21.61123802674067 26-27 21.426251766981625 27.79305855219106 29.21467896733874 21.566010713488577 28-29 21.423014757312682 27.85444395946263 29.20960754969767 21.51293373352702 30-31 21.725523851826388 27.69738073156786 29.150479685222034 21.426615731383716 32-33 21.52788508831386 27.771415076533717 29.00521505961197 21.69548477554045 34-35 21.868144850341345 27.821635645336386 28.993230779534755 21.316988724787514 36-37 21.7323260301871 27.60063203237399 28.987844128940853 21.67919780849806 38-39 21.635476337296417 27.85592644160521 29.017878983196194 21.49071823790218 40-41 21.830011805303982 27.6018346945015 28.99490891616598 21.573244584028547 42-43 21.686786198606114 27.765233032234644 28.820969144513235 21.727011624646007 44-45 21.78194460790849 27.839007844228775 28.757523916726118 21.62152363113662 46-47 21.755981533834184 27.79895025187559 28.60251258262285 21.842555631667377 48-49 21.765288893517003 27.86464996578501 28.65574142393859 21.714319716759395 50-51 21.68098015559377 28.039620019957262 28.4760991601179 21.80330066433107 52-53 21.745939071192694 28.01849358879005 28.352144259661436 21.883423080355815 54-55 21.705078956611832 27.923817048367155 28.413609848403677 21.957494146617336 56-57 21.71828167732042 27.990248171765174 28.278195855864386 22.01327429505002 58-59 21.67442581784237 28.076974408221815 28.342461892676173 21.90613788125964 60-61 21.883128252580246 28.083593533550893 28.21925551695477 21.814022696914083 62-63 21.76092392172598 27.979822185564558 28.32129574932103 21.937958143388432 64-65 21.67038197037417 27.86477780626177 28.36474622618026 22.1000939971838 66-67 21.64249861604937 27.87791140486779 28.288675382769163 22.190914596313675 68-69 21.6598490843634 27.91900266387276 28.223230307961522 22.197917943802317 70-71 21.699212727144378 27.726289860564652 28.22839458011495 22.346102832176015 72-73 21.748812031639524 27.778731372396038 28.21195436120926 22.26050223475518 74-75 21.689478631430724 27.850195239209828 28.2249021946299 22.235423934729546 76-77 21.65128530931761 27.779660198322915 28.18734047414706 22.381714018212417 78-79 21.629829430406787 27.798942624564845 28.176380328209927 22.394847616818435 80-81 21.646845521387146 27.80977273487221 28.155778969151836 22.387602774588807 82-83 21.750985484308416 27.764520335714842 28.237627109828097 22.24686707014865 84-85 21.807067993773153 27.76461321830753 28.10926336673391 22.319055421185404 86-87 21.652418476948398 27.689285435637935 28.223899062628877 22.43439702478479 88-89 21.782082576340205 27.627815735797324 28.12390166334147 22.466200024521005 90-91 21.724402486281242 27.63619374565774 28.188269300073937 22.451134467987085 92-93 21.80812685532979 27.561367528988654 28.18637449518311 22.444131120498444 94-95 21.62199013958396 27.57572717781815 28.204653789424018 22.59762889317387 96-97 21.589889915551147 27.47628707408687 28.245224905909932 22.688598104452048 98-99 21.64376181930992 27.419238585658185 28.1103965343647 22.826603060667196 100-101 21.641941320493245 27.41138071831682 28.09096549597447 22.85571246521547 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 125.0 1 88.0 2 63.5 3 88.0 4 87.5 5 77.5 6 155.0 7 300.5 8 490.5 9 687.5 10 777.5 11 811.0 12 897.5 13 1061.5 14 1218.5 15 1388.0 16 1593.0 17 1792.0 18 1997.0 19 2256.5 20 2666.5 21 3220.5 22 4024.0 23 5228.0 24 7017.0 25 9628.5 26 13188.5 27 17683.5 28 23351.5 29 30607.5 30 38342.5 31 46085.5 32 54541.5 33 62532.5 34 70385.0 35 77770.5 36 85160.0 37 92473.0 38 96748.5 39 99335.0 40 101400.0 41 102812.0 42 103907.5 43 104267.5 44 104581.0 45 105274.5 46 103821.0 47 101313.5 48 99635.0 49 97109.0 50 93920.5 51 89856.5 52 83866.0 53 77318.5 54 71379.0 55 65014.5 56 57973.5 57 50709.5 58 44071.5 59 37941.0 60 31567.0 61 25525.0 62 19906.0 63 15063.0 64 11504.5 65 8614.5 66 6434.0 67 5027.5 68 4030.5 69 3121.0 70 2314.5 71 1684.5 72 1178.5 73 852.5 74 655.0 75 484.5 76 356.5 77 288.5 78 216.5 79 167.5 80 120.5 81 85.0 82 68.5 83 50.0 84 40.0 85 34.5 86 28.5 87 22.5 88 15.5 89 15.0 90 13.5 91 9.0 92 5.0 93 5.0 94 4.5 95 1.0 96 2.0 97 2.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03488670181343974 2 0.1415159182187348 3 0.0 4 0.30075383512225207 5 0.21389003444086535 6 0.09533469313449028 7 0.10889555166687101 8 0.11082750959477182 9 0.11023306100157158 10-11 0.11448708374666088 12-13 0.1668542895038955 14-15 0.12555868879501553 16-17 0.11903833078835029 18-19 0.12769498842682894 20-21 0.10166928595578045 22-23 0.10191078069676807 24-25 0.09500031580081514 26-27 0.1252057349428029 28-29 0.11288950315243519 30-31 0.1282894370200292 32-33 0.10114914343673023 34-35 0.15000538719037587 36-37 0.1386922874010336 38-39 0.1351441723603696 40-41 0.10952715329714628 42-43 0.11041882618694665 44-45 0.0890001003132001 46-47 0.09451732631883994 48-49 0.09994166973179223 50-51 0.08632508164379897 52-53 0.09399718379978972 54-55 0.30809155994456766 56-57 0.32661234892646296 58-59 0.33980167708809356 60-61 0.1626002667588062 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2691570.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.68890396765748 #Duplication Level Percentage of deduplicated Percentage of total 1 87.10892581945464 67.6739697271341 2 8.81611472183347 13.698285799847435 3 2.0260223928004097 4.72198377331784 4 0.7675794099535452 2.385296122697287 5 0.35636908935266787 1.384296197988048 6 0.2225979987747683 1.0376036730123428 7 0.14087158042126938 0.7660911078184093 8 0.09686979430668817 0.6020566517807224 9 0.06952797253866687 0.4861396783460174 >10 0.3694524767715573 5.040909278923573 >50 0.018122712469868414 0.9476161009910542 >100 0.007352489592488847 0.8999614762553805 >500 4.8385432502400496E-5 0.024771883933951803 >1k 1.4515629750670692E-4 0.3310185279539246 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4078 0.15151008519191403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2976 0.11056743833524672 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.43060741500314E-5 0.0 5 0.0 0.0 0.0 7.43060741500314E-5 0.0 6 0.0 0.0 0.0 7.43060741500314E-5 0.0 7 0.0 0.0 0.0 1.114591112250471E-4 0.0 8 0.0 0.0 0.0 1.114591112250471E-4 0.0 9 0.0 0.0 0.0 1.486121483000628E-4 0.0 10-11 0.0 0.0 0.0 1.486121483000628E-4 0.0 12-13 0.0 0.0 0.0 1.6718866683757065E-4 0.0 14-15 0.0 3.71530370750157E-5 0.0 1.857651853750785E-4 5.5729555612523554E-5 16-17 0.0 3.71530370750157E-5 0.0 1.857651853750785E-4 7.43060741500314E-5 18-19 0.0 3.71530370750157E-5 0.0 1.857651853750785E-4 1.1145911122504711E-4 20-21 0.0 3.71530370750157E-5 0.0 2.2291822245009416E-4 1.486121483000628E-4 22-23 0.0 7.43060741500314E-5 0.0 2.6007125952510986E-4 1.6718866683757065E-4 24-25 0.0 1.1145911122504711E-4 0.0 3.9010688928766486E-4 1.857651853750785E-4 26-27 0.0 1.486121483000628E-4 0.0 9.288259268753925E-4 1.857651853750785E-4 28-29 0.0 1.486121483000628E-4 0.0 0.002452100446951036 1.857651853750785E-4 30-31 0.0 1.857651853750785E-4 0.0 0.0077464082301407725 1.857651853750785E-4 32-33 0.0 1.857651853750785E-4 0.0 0.02195744491133428 1.857651853750785E-4 34-35 0.0 2.2291822245009416E-4 0.0 0.0440077724153561 1.857651853750785E-4 36-37 0.0 2.6007125952510986E-4 0.0 0.07887589771025832 2.229182224500942E-4 38-39 0.0 2.7864777806261773E-4 0.0 0.126598973833116 2.229182224500942E-4 40-41 0.0 3.343773336751413E-4 0.0 0.17642119655071203 2.229182224500942E-4 42-43 0.0 3.343773336751413E-4 0.0 0.2310175845324476 2.229182224500942E-4 44-45 0.0 3.343773336751413E-4 0.0 0.3003637282329644 2.229182224500942E-4 46-47 0.0 3.343773336751413E-4 0.0 0.3841066738000498 2.229182224500942E-4 48-49 0.0 3.343773336751413E-4 0.0 0.47106335707412406 2.4149474098760204E-4 50-51 0.0 3.343773336751413E-4 0.0 0.5550663739007344 2.6007125952510986E-4 52-53 0.0 3.343773336751413E-4 0.0 0.642840423990459 2.6007125952510986E-4 54-55 1.857651853750785E-5 3.343773336751413E-4 0.0 0.7425220224627262 2.6007125952510986E-4 56-57 3.71530370750157E-5 3.343773336751413E-4 0.0 0.8594797831748756 2.6007125952510986E-4 58-59 3.71530370750157E-5 3.343773336751413E-4 0.0 0.988140750565655 2.6007125952510986E-4 60-61 3.71530370750157E-5 3.343773336751413E-4 0.0 1.1209071285532235 2.6007125952510986E-4 62-63 7.43060741500314E-5 3.343773336751413E-4 0.0 1.2547509446159677 2.6007125952510986E-4 64-65 7.43060741500314E-5 4.458364449001884E-4 0.0 1.3999635900236664 2.6007125952510986E-4 66-67 7.43060741500314E-5 4.458364449001884E-4 0.0 1.564180013895236 2.6007125952510986E-4 68-69 7.43060741500314E-5 4.458364449001884E-4 0.0 1.7494064802327267 2.6007125952510986E-4 70-71 9.288259268753925E-5 4.6441296343769625E-4 0.0 1.9447571491731592 2.6007125952510986E-4 72-73 1.486121483000628E-4 4.8298948197520407E-4 0.0 2.1459036918972942 2.6007125952510986E-4 74-75 1.486121483000628E-4 5.387190375877276E-4 0.0 2.3574345084838964 2.6007125952510986E-4 76-77 1.486121483000628E-4 5.572955561252355E-4 0.0 2.584885401457142 2.6007125952510986E-4 78-79 1.486121483000628E-4 5.572955561252355E-4 0.0 2.836671533714523 2.6007125952510986E-4 80-81 1.486121483000628E-4 5.572955561252355E-4 0.0 3.110433687401777 2.7864777806261773E-4 82-83 1.486121483000628E-4 5.758720746627433E-4 0.0 3.391942249319171 2.972242966001256E-4 84-85 1.6718866683757065E-4 5.944485932002512E-4 0.0 3.6793024145758793 3.343773336751413E-4 86-87 1.857651853750785E-4 5.944485932002512E-4 0.0 3.9844774611100586 3.529538522126491E-4 88-89 1.857651853750785E-4 7.059077044252982E-4 0.0 4.318761912192512 3.71530370750157E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2860 0.0 47.662704 1 GTATCAA 5040 0.0 39.15151 1 ATCAACG 5605 0.0 34.234375 3 CAACGCA 5675 0.0 34.18762 5 TCAACGC 5675 0.0 33.984978 4 AACGCAG 5995 0.0 32.741676 6 TATCAAC 6365 0.0 30.70239 2 ACGCAGA 6875 0.0 28.006563 7 CGCAGAG 7020 0.0 27.224909 8 GCAGAGT 7800 0.0 22.856733 9 GTACATG 5975 0.0 21.93681 1 TACATGG 6005 0.0 19.922667 2 AGAGTAC 7025 0.0 19.35482 10-11 GAGTACT 4625 0.0 19.322124 12-13 CAGAGTA 8010 0.0 18.963032 10-11 ACATGGG 6280 0.0 18.878727 3 TATACCG 355 7.8216544E-11 18.75315 5 GTGGTAT 1600 0.0 17.873514 1 CCTATAC 910 0.0 17.789778 3 TGTACCG 375 1.6916601E-10 17.752981 5 >>END_MODULE