FastQCFastQC Report
Sat 18 Jun 2016
SRR1931124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2691570
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46700.1735046831403233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36210.13453114724863183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.048.2006951
GTATCAA52750.041.094581
ATCAACG62250.033.7964173
TCAACGC62400.033.6384434
CAACGCA63300.033.1595575
AACGCAG66500.031.5633226
TATCAAC67850.031.4969832
ACGCAGA74900.027.57977
CGCAGAG76700.026.5609748
GTACATG61500.022.7255521
TACATGG61750.021.8417632
GCAGAGT90200.021.7433079
ACATGGG62200.021.3019873
AGAGTAC77650.020.91542810-11
GAGTACT52150.020.89827712-13
CAGAGTA87650.018.6917210-11
AGTACTT59750.017.88243712-13
GTGGTAT15450.017.8460331
GTACTTT62900.017.47762514-15
CATGGGG37900.017.0411134
Produced by FastQC (version 0.11.5)