FastQCFastQC Report
Sat 18 Jun 2016
SRR1931123_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931123_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733869
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52800.19313288237293008No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38790.1418868277887492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31200.056.293361
GTATCAA59400.040.474551
ATCAACG68050.033.584663
TCAACGC68850.033.482094
CAACGCA68300.033.4781955
AACGCAG71550.032.2066656
TATCAAC74200.031.9493332
ACGCAGA81350.027.809797
CGCAGAG81450.027.3068438
GTACATG65950.024.0120871
GCAGAGT90900.023.2114099
TACATGG68500.022.1378422
GAGTACT55700.021.330912-13
ACATGGG67150.020.8623283
CAGAGTA91550.020.52307110-11
AGAGTAC81400.020.27367410-11
GTGGTAT15100.020.0213951
GTACTTT64550.018.6150314-15
TGGTATC15300.017.8781342
GTATAGG10550.017.28471
Produced by FastQC (version 0.11.5)