FastQCFastQC Report
Sat 18 Jun 2016
SRR1931123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2733869
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69390.25381611189124276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53270.19485205765162852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35390.1294502406662499No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34400.053.494661
GTATCAA65200.043.466691
ATCAACG80250.035.150143
TCAACGC81750.034.388384
CAACGCA81700.034.3512955
AACGCAG86200.032.7783746
TATCAAC86950.032.550852
ACGCAGA99950.027.9365167
CGCAGAG101300.027.4704598
GCAGAGT114050.023.8581669
GTGGTAT17500.023.3678261
GTACATG73100.022.3768521
TGGTATC17200.021.8114382
GAGTACT68750.021.68875712-13
AGAGTAC101850.021.19095610-11
TACATGG77250.020.7780082
ACATGGG73000.020.686643
CCGTCGA2151.082677E-619.8784149
CAGAGTA111550.019.83782610-11
GTACTTT77750.018.90331514-15
Produced by FastQC (version 0.11.5)