##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931123_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2733869 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.24164983764767 31.0 31.0 34.0 30.0 34.0 2 31.252132051682068 31.0 31.0 34.0 28.0 34.0 3 31.411433759262056 31.0 31.0 34.0 28.0 34.0 4 35.04737717864316 35.0 35.0 37.0 32.0 37.0 5 34.92069993112325 35.0 35.0 37.0 32.0 37.0 6 35.033097416152714 36.0 35.0 37.0 32.0 37.0 7 34.89747131263422 36.0 35.0 37.0 32.0 37.0 8 34.944184596994226 36.0 35.0 37.0 32.0 37.0 9 36.355287323569634 38.0 35.0 39.0 32.0 39.0 10-11 36.2216871035152 38.0 35.0 39.0 31.5 39.0 12-13 36.16159003961053 38.0 35.0 39.0 32.0 39.0 14-15 37.09736055385243 39.0 36.0 40.0 31.0 41.0 16-17 37.14852558772934 39.0 36.0 40.0 31.0 41.0 18-19 37.204991899758184 39.0 36.0 40.0 31.5 41.0 20-21 37.179424471326165 39.0 36.0 40.0 31.0 41.0 22-23 37.06322834049473 39.0 36.0 40.0 31.0 41.0 24-25 36.93797325329048 39.0 36.0 40.0 31.0 41.0 26-27 36.68017048366253 39.0 35.5 40.0 30.0 41.0 28-29 36.39804339564185 38.0 35.0 40.0 30.0 41.0 30-31 36.18767450086306 38.0 35.0 40.0 29.5 41.0 32-33 36.02498784689391 38.0 34.5 40.0 29.5 41.0 34-35 35.78601480173337 38.0 34.0 40.0 28.5 41.0 36-37 36.05570822888734 38.0 35.0 40.0 30.0 41.0 38-39 36.060382556735526 38.0 35.0 40.0 30.0 41.0 40-41 35.90178772282066 38.0 35.0 40.0 29.0 41.0 42-43 35.74413989843698 38.0 34.5 40.0 28.5 41.0 44-45 35.53861267675957 38.0 34.0 40.0 27.5 41.0 46-47 35.33223354886427 38.0 34.0 40.0 27.0 41.0 48-49 35.08928884302796 38.0 34.0 40.0 26.0 41.0 50-51 34.762296584071876 38.0 33.0 40.0 25.5 41.0 52-53 34.640291469708316 38.0 33.0 40.0 25.0 41.0 54-55 34.4182530326069 38.0 33.0 40.0 24.0 41.0 56-57 34.19696792348134 37.5 33.0 40.0 24.0 41.0 58-59 33.95861304985718 37.0 32.5 40.0 23.5 41.0 60-61 33.68168482103569 37.0 32.0 40.0 23.0 41.0 62-63 33.42197724177713 36.5 32.0 39.5 22.5 41.0 64-65 33.18695701952069 36.0 31.5 39.0 22.0 41.0 66-67 32.94169160994912 36.0 31.0 39.0 22.0 40.5 68-69 32.61602677377738 35.5 31.0 39.0 21.5 40.0 70-71 31.799016704896978 35.0 30.0 38.0 18.5 40.0 72-73 31.758264386479382 35.0 30.0 38.0 20.0 40.0 74-75 31.444384131061142 35.0 30.0 37.0 18.5 39.5 76-77 30.713988856086374 34.0 29.5 36.0 18.0 39.0 78-79 30.72905724451318 34.0 30.0 36.0 18.0 39.0 80-81 30.459994791264688 34.0 30.0 36.0 16.0 38.5 82-83 30.110029229637558 34.0 29.0 35.5 12.5 37.0 84-85 29.73620480717986 34.0 29.0 35.0 9.0 37.0 86-87 29.399370818426192 34.0 29.0 35.0 7.0 36.5 88-89 29.07915960859866 34.0 29.0 35.0 4.0 36.0 90-91 28.76220202943155 34.0 29.0 35.0 2.0 36.0 92-93 28.413708923141527 33.5 29.0 35.0 2.0 35.0 94-95 28.02739048579138 33.0 27.5 35.0 2.0 35.0 96-97 27.651060456810477 33.0 27.0 35.0 2.0 35.0 98-99 27.1662277892613 33.0 26.5 35.0 2.0 35.0 100-101 26.47619618935655 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7534845100580227 1101 2 0.6357137079824575 1101 3 0.4837171726159326 1101 4 0.8339065949894291 1101 5 0.7860725231027459 1101 6 0.830464574709822 1101 7 0.7798648451429315 1101 8 0.7249764143603343 1101 9 1.0852932027243796 1101 10-11 0.9000617691109838 1101 12-13 1.012803495121858 1101 14-15 1.2890898329733176 1101 16-17 1.0519135392589476 1101 18-19 0.920954745060186 1101 20-21 0.8326860983184474 1101 22-23 0.7762077920690089 1101 24-25 0.7071081791479941 1101 26-27 0.9611721936990563 1101 28-29 0.8340623561959646 1101 30-31 0.9183029827611122 1101 32-33 1.013562283315828 1101 34-35 0.8088778161295522 1101 36-37 1.0968014022015353 1101 38-39 0.9252664597988343 1101 40-41 0.945070752242863 1101 42-43 0.9769422610603797 1101 44-45 0.8274105745845048 1101 46-47 0.9067485483531712 1101 48-49 0.675110343273964 1101 50-51 0.6588409558660899 1101 52-53 0.5479190037606969 1101 54-55 0.49103170945600993 1101 56-57 0.3897937178370512 1101 58-59 0.6116138483043656 1101 60-61 0.6033913683448588 1101 62-63 0.5123696793270014 1101 64-65 0.37462407107623363 1101 66-67 0.08200834425986869 1101 68-69 0.147051609782487 1101 70-71 0.4558968193023034 1101 72-73 -0.04099825746944319 1101 74-75 -0.31965891465819496 1101 76-77 -0.22651147973672536 1101 78-79 -0.02779109839317684 1101 80-81 -0.2605970021834665 1101 82-83 -0.2783441804101763 1101 84-85 -0.1979720315316733 1101 86-87 -0.24520600338568244 1101 88-89 -0.3613857418446642 1101 90-91 -0.2853743853644133 1101 92-93 -0.5720998149205805 1101 94-95 -0.3795456062413045 1101 96-97 -0.41177243489361715 1101 98-99 -0.5059237830417231 1101 100-101 -0.6582823596454723 1102 1 1.026612445245398 1102 2 0.878658946880094 1102 3 0.6731407734924879 1102 4 1.0074102676937144 1102 5 1.0339199126407834 1102 6 0.8985179995212391 1102 7 0.9623904937134995 1102 8 0.9831317711768435 1102 9 1.388989422043764 1102 10-11 1.301248418944077 1102 12-13 1.382689541528208 1102 14-15 1.6620662198572091 1102 16-17 1.4155285332146192 1102 18-19 1.3370228263174013 1102 20-21 1.3176959570270554 1102 22-23 0.9151837270954246 1102 24-25 0.658364242337619 1102 26-27 1.0715803199240597 1102 28-29 1.2155657505761681 1102 30-31 1.4725221495661032 1102 32-33 1.4994999378820992 1102 34-35 1.4621021839063317 1102 36-37 1.603535860064774 1102 38-39 1.4272213511769891 1102 40-41 1.4369218295750414 1102 42-43 1.4965159473623046 1102 44-45 1.5564463786309304 1102 46-47 1.6097930324224734 1102 48-49 1.3327254202238734 1102 50-51 1.2600833753071896 1102 52-53 1.3149962194132385 1102 54-55 1.0325166480989623 1102 56-57 1.2017325427058125 1102 58-59 1.2482088549019892 1102 60-61 1.6365899408440967 1102 62-63 1.5314701098910035 1102 64-65 1.4434749740366968 1102 66-67 1.0481409137109452 1102 68-69 1.0241568338920644 1102 70-71 1.436926957944582 1102 72-73 0.9481617528922577 1102 74-75 0.8503247128435767 1102 76-77 0.9972394570920926 1102 78-79 1.0210454314755246 1102 80-81 0.8696327241703017 1102 82-83 0.9123579507810682 1102 84-85 0.9769076058757555 1102 86-87 0.9449089017074783 1102 88-89 0.7594364836749747 1102 90-91 0.7360536351645024 1102 92-93 0.6620815685513293 1102 94-95 0.6096358023803674 1102 96-97 0.7714987575574277 1102 98-99 0.7979058557908587 1102 100-101 0.6572290105641265 1103 1 0.687978848066944 1103 2 0.4155856178583264 1103 3 0.3912528242001052 1103 4 0.7074699242635631 1103 5 0.7623515033262862 1103 6 0.7894077476632333 1103 7 0.6619321860033125 1103 8 0.6692610106375696 1103 9 0.9468761464251969 1103 10-11 0.9009147845242254 1103 12-13 1.027203736668966 1103 14-15 1.295189586414864 1103 16-17 1.1036964943188252 1103 18-19 0.987479945472451 1103 20-21 0.8925114778029766 1103 22-23 0.7407357812979498 1103 24-25 0.4460266628469043 1103 26-27 0.8954335665418824 1103 28-29 0.722350620781917 1103 30-31 1.0670328976850811 1103 32-33 1.0833829479545685 1103 34-35 0.9240460889341193 1103 36-37 1.2014982697732535 1103 38-39 0.9343442710413044 1103 40-41 1.0284325458721 1103 42-43 1.1189126991025447 1103 44-45 1.1010268362505116 1103 46-47 1.0863956782691062 1103 48-49 0.8389099535579305 1103 50-51 0.8154851508009529 1103 52-53 0.3795011832305306 1103 54-55 0.4658491918870453 1103 56-57 0.5712625990195974 1103 58-59 0.7361114186345006 1103 60-61 0.8975452518910956 1103 62-63 0.6483928342729541 1103 64-65 0.6734068348695601 1103 66-67 0.5052096452065129 1103 68-69 0.4787766545087919 1103 70-71 0.6874673056204763 1103 72-73 0.35253929258142236 1103 74-75 0.06604382705177869 1103 76-77 0.1992759287434822 1103 78-79 0.2972054771838408 1103 80-81 0.15921984130035582 1103 82-83 0.1663375236495206 1103 84-85 0.2399587358230555 1103 86-87 0.142538884618979 1103 88-89 -0.06654306902405693 1103 90-91 -0.05225094410328879 1103 92-93 -0.33843673471056945 1103 94-95 -0.41868342005626147 1103 96-97 -0.3853957542118742 1103 98-99 -0.5313094482400622 1103 100-101 -0.5764319685095494 1104 1 0.4542889068613647 1104 2 0.39457958389035497 1104 3 0.15301907007439652 1104 4 0.6552016643662313 1104 5 0.39886833659706866 1104 6 0.5279149627551618 1104 7 0.5375220451185356 1104 8 0.456641746065344 1104 9 0.730045278919377 1104 10-11 0.5803934164371967 1104 12-13 0.6609791711316078 1104 14-15 1.0340345927543808 1104 16-17 0.7695989794419518 1104 18-19 0.6097674161536517 1104 20-21 0.5630017178642603 1104 22-23 0.3133502159967989 1104 24-25 0.4215740543966575 1104 26-27 0.09146088739787217 1104 28-29 0.4916862645351898 1104 30-31 0.7657023208838254 1104 32-33 0.9597080965629274 1104 34-35 0.7717470113124847 1104 36-37 1.019574962214655 1104 38-39 0.526676822067877 1104 40-41 0.6407097332880554 1104 42-43 0.9297796726191692 1104 44-45 0.8327233717576874 1104 46-47 0.9986538394160149 1104 48-49 0.7785597152651533 1104 50-51 0.7966038279454466 1104 52-53 0.4793815984646912 1104 54-55 0.6565394110311047 1104 56-57 0.6532859569303326 1104 58-59 0.7131189763700405 1104 60-61 0.995531287159416 1104 62-63 0.0451453983521759 1104 64-65 -1.0174955554970886 1104 66-67 -0.4473486986517372 1104 68-69 0.29839058868515167 1104 70-71 0.9097168903684043 1104 72-73 0.4665060193521633 1104 74-75 0.19046046552405116 1104 76-77 -0.0503069240564642 1104 78-79 0.04678995438066025 1104 80-81 -0.0967915621323101 1104 82-83 -0.24074968242992156 1104 84-85 -0.3394463442324316 1104 86-87 -0.06413821123097563 1104 88-89 -0.04532024055007611 1104 90-91 -0.06835865094596727 1104 92-93 0.057863976304602716 1104 94-95 0.10530340300289254 1104 96-97 0.23355847490747195 1104 98-99 0.2572563263592116 1104 100-101 0.023119152322212955 1105 1 -0.31348429896207364 1105 2 -1.759383925945027 1105 3 -0.38316227035596384 1105 4 0.4711811534279349 1105 5 0.6028731375196585 1105 6 0.11158908735076523 1105 7 -0.07228743784550318 1105 8 -0.07274606859729715 1105 9 0.49245079498823685 1105 10-11 0.8836777909641285 1105 12-13 1.0149671261799043 1105 14-15 1.1884542397529785 1105 16-17 0.8084557013904359 1105 18-19 0.5913777240905702 1105 20-21 0.9516286608746825 1105 22-23 0.3629090703479889 1105 24-25 0.0034628144291417584 1105 26-27 0.9558626143071507 1105 28-29 0.48356891781192957 1105 30-31 0.9112067916596231 1105 32-33 0.9472752703709162 1105 34-35 0.5640922541164031 1105 36-37 0.7455070130113839 1105 38-39 0.7284982279091352 1105 40-41 0.8134064970409867 1105 42-43 1.1650024706754394 1105 44-45 1.1165165290787442 1105 46-47 1.2024983923172172 1105 48-49 1.0322753320474831 1105 50-51 0.7037447607117144 1105 52-53 0.9198757959856465 1105 54-55 -0.07899165540874264 1105 56-57 0.26881144109005106 1105 58-59 -0.47659489943615085 1105 60-61 0.28820217981289176 1105 62-63 0.8295945291677214 1105 64-65 1.204156915631991 1105 66-67 0.9021442779222966 1105 68-69 0.8777804598177426 1105 70-71 0.33008148076507027 1105 72-73 -0.08926473851411387 1105 74-75 -0.14458535484981638 1105 76-77 0.05637826566123039 1105 78-79 -0.437653178818568 1105 80-81 -0.34199569229200577 1105 82-83 -0.27583098827924957 1105 84-85 -0.18891941296460857 1105 86-87 -0.35038730906214965 1105 88-89 -0.8666912683545647 1105 90-91 -0.9529306596310434 1105 92-93 -0.554194587513372 1105 94-95 -0.7645967536122029 1105 96-97 -0.8678557094601622 1105 98-99 -1.579393212152759 1105 100-101 -0.9625737537558265 1106 1 0.37405382095994355 1106 2 0.423429039472623 1106 3 -0.25715616750801473 1106 4 0.44703010954260947 1106 5 0.1965991874561297 1106 6 0.582718722767595 1106 7 0.49942731692643605 1106 8 0.5246647736808256 1106 9 0.9424641097937112 1106 10-11 0.2400338604335488 1106 12-13 0.3867798660237014 1106 14-15 0.11340718038038489 1106 16-17 -0.27324195460898437 1106 18-19 0.44792453615552574 1106 20-21 0.5695963502800723 1106 22-23 0.5154556755987869 1106 24-25 0.44395621490691894 1106 26-27 0.7574118445420979 1106 28-29 0.6978934751175316 1106 30-31 0.3830245302748878 1106 32-33 0.3916015961206085 1106 34-35 -0.9861598120806008 1106 36-37 -0.7042821577965199 1106 38-39 -0.6319689021371602 1106 40-41 0.6084405664710602 1106 42-43 0.7414339724070445 1106 44-45 0.5838635701034462 1106 46-47 0.2211347160216448 1106 48-49 0.08799464725468198 1106 50-51 0.7049745500037687 1106 52-53 0.08517853393974661 1106 54-55 0.6118155123491107 1106 56-57 0.3192575671451152 1106 58-59 0.8814211739590831 1106 60-61 1.033572196426661 1106 62-63 0.8899311306528546 1106 64-65 0.932021223122554 1106 66-67 0.6790779331292285 1106 68-69 0.6870485596028288 1106 70-71 1.0728389815532076 1106 72-73 0.49846141299921953 1106 74-75 0.43622905140366086 1106 76-77 0.5891591680206929 1106 78-79 0.6019970307180351 1106 80-81 0.5942966239671108 1106 82-83 0.5719849031927318 1106 84-85 0.5615285318654237 1106 86-87 0.5590696115939622 1106 88-89 0.5997921405284714 1106 90-91 0.8324866085277662 1106 92-93 0.4090295537388684 1106 94-95 0.49929416884758737 1106 96-97 0.8295509233261953 1106 98-99 0.8328903593447663 1106 100-101 0.7885129928881334 1107 1 0.47229729369442097 1107 2 0.4781472763237318 1107 3 0.18694547700663122 1107 4 -0.9996271486842545 1107 5 0.21338736554512394 1107 6 0.4979726664282822 1107 7 0.49637628437476167 1107 8 0.5296127061793854 1107 9 0.855676305454395 1107 10-11 0.8601344311797945 1107 12-13 0.8047453796752677 1107 14-15 1.1161826326936932 1107 16-17 0.9197583714996469 1107 18-19 0.8686687102721962 1107 20-21 0.32834193245455623 1107 22-23 0.5720606265148618 1107 24-25 0.4407953031565981 1107 26-27 0.8703651744352214 1107 28-29 0.854995184276433 1107 30-31 1.077461827670085 1107 32-33 1.20861258837148 1107 34-35 1.1294174250558768 1107 36-37 1.257761276087237 1107 38-39 1.125335939172409 1107 40-41 0.38148820961037444 1107 42-43 0.22597104171868665 1107 44-45 -0.1160554160436007 1107 46-47 0.48550366695119607 1107 48-49 0.7360994155467253 1107 50-51 -1.0296210279760487 1107 52-53 0.579958550169799 1107 54-55 0.8949651442377515 1107 56-57 0.8358438722830215 1107 58-59 1.1228429370020976 1107 60-61 1.3246134806883205 1107 62-63 1.280255609540724 1107 64-65 1.2338980400392714 1107 66-67 1.1278063340468165 1107 68-69 1.1576820605032836 1107 70-71 1.4775462866271063 1107 72-73 1.1266598400744776 1107 74-75 0.8504800867143452 1107 76-77 0.9849811759098586 1107 78-79 0.9922863803438631 1107 80-81 0.9840846998764725 1107 82-83 0.9796860154108309 1107 84-85 0.8300774238580253 1107 86-87 0.8534126855712145 1107 88-89 0.9334909358193535 1107 90-91 1.144450736899394 1107 92-93 1.1272431858495153 1107 94-95 1.2486189732024613 1107 96-97 1.2997893804361738 1107 98-99 1.1762948965375948 1107 100-101 0.8918834530926958 1108 1 0.5106251019806578 1108 2 0.45347715309411285 1108 3 0.2452269624858694 1108 4 0.6432806940495652 1108 5 0.49423493527397966 1108 6 0.498141941453099 1108 7 0.5233898161069206 1108 8 0.6549726664505116 1108 9 0.7394136383056349 1108 10-11 0.6789198510048706 1108 12-13 0.8140207420967585 1108 14-15 1.065031237904087 1108 16-17 0.7917748254098029 1108 18-19 0.6546366134138211 1108 20-21 0.5963735885368138 1108 22-23 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1.5400472968074261 2311 80-81 1.4146160809372361 2311 82-83 1.3661398932269613 2311 84-85 1.463559034707 2311 86-87 1.3798606422470279 2311 88-89 1.3076324982533762 2311 90-91 1.6109076342611814 2311 92-93 1.7959038797395408 2311 94-95 2.0680432622233056 2311 96-97 2.013039288336099 2311 98-99 2.1471686657680316 2311 100-101 2.058417686995039 2312 1 -0.018840041822510756 2312 2 0.00721982363708662 2312 3 -0.24042579136563447 2312 4 0.3326900984030985 2312 5 -0.7207457683739378 2312 6 -0.3020002632916743 2312 7 0.18108375421628864 2312 8 -2.030259285622776 2312 9 -4.940867203538435 2312 10-11 -0.4094868545651664 2312 12-13 -5.868934581160985 2312 14-15 -1.7225941432878074 2312 16-17 -2.183855199734076 2312 18-19 -2.3396822118286664 2312 20-21 -3.4334009683385673 2312 22-23 -2.1123232445766646 2312 24-25 -2.090751944853835 2312 26-27 -2.5426145062599375 2312 28-29 -5.7117501694367085 2312 30-31 -6.622508457252394 2312 32-33 -1.6527995053020632 2312 34-35 0.6264925902001437 2312 36-37 1.157836419450824 2312 38-39 1.243222600784577 2312 40-41 -2.188951044577479 2312 42-43 -4.725940057822157 2312 44-45 -3.11418824088436 2312 46-47 0.35750998957292524 2312 48-49 -1.241253857830948 2312 50-51 -7.271679116026025 2312 52-53 -1.5130309733206744 2312 54-55 0.9321932616501698 2312 56-57 1.4266646618432262 2312 58-59 1.484532475116744 2312 60-61 1.4985947410017317 2312 62-63 1.6941303726757724 2312 64-65 1.4180726609775576 2312 66-67 1.2620438078192464 2312 68-69 1.673494578589029 2312 70-71 1.8798905106487105 2312 72-73 1.314061632521149 2312 74-75 1.391119474282963 2312 76-77 1.4828936009609635 2312 78-79 1.455913154821463 2312 80-81 1.224761010892209 2312 82-83 0.9490315161954577 2312 84-85 1.131035410990826 2312 86-87 1.5637759873986177 2312 88-89 1.4102250154350173 2312 90-91 1.5893150069301036 2312 92-93 1.9051395761676915 2312 94-95 2.001971124356267 2312 96-97 2.02189713881992 2312 98-99 2.2024511656929278 2312 100-101 2.1406066900758844 2313 1 -0.6280499478099202 2313 2 -0.1385967547483382 2313 3 -0.20406870991578785 2313 4 -2.181910972064749 2313 5 -1.9553889919796958 2313 6 -2.838884258965429 2313 7 -3.5487063120639704 2313 8 -5.4032452253327 2313 9 -2.8046182781661315 2313 10-11 -1.1023582597622053 2313 12-13 -3.6840223539777597 2313 14-15 -3.1297185782416364 2313 16-17 -6.41025498896839 2313 18-19 -3.854616070227408 2313 20-21 -4.663558487959193 2313 22-23 -3.410404167508524 2313 24-25 -1.4217650067801273 2313 26-27 -3.249171815114863 2313 28-29 -4.232591013608758 2313 30-31 -3.796549612026517 2313 32-33 -8.017285486610476 2313 34-35 -4.704270880777976 2313 36-37 -4.5512999958685185 2313 38-39 -3.762344663975952 2313 40-41 -2.04571357265646 2313 42-43 -3.8062182130677265 2313 44-45 -5.419347840429552 2313 46-47 -3.812905163370857 2313 48-49 -2.361348702391254 2313 50-51 -1.2103074751042016 2313 52-53 -2.0319998853814667 2313 54-55 -4.0473610798457145 2313 56-57 -7.090168860994346 2313 58-59 -3.0677152300331443 2313 60-61 -9.304512666302966 2313 62-63 -4.499822593296663 2313 64-65 -4.370447542023079 2313 66-67 -5.702001229480732 2313 68-69 -7.486014836536182 2313 70-71 -6.4267042368484475 2313 72-73 -2.8037660942599594 2313 74-75 -0.9021013582415094 2313 76-77 -4.668476376021204 2313 78-79 -2.9521680454336767 2313 80-81 -0.7321360189755062 2313 82-83 -0.23733665818541638 2313 84-85 -0.5779346103630658 2313 86-87 -0.2619609992066714 2313 88-89 -6.74551973622199 2313 90-91 -1.9721952956295894 2313 92-93 -0.7885499326927885 2313 94-95 -0.3616898202068093 2313 96-97 -0.2229446482512678 2313 98-99 -0.13128659741752813 2313 100-101 0.3976784336073216 2314 1 -28.702816302950108 2314 2 -14.866704820404507 2314 3 -5.913642258961577 2314 4 -4.156151135419812 2314 5 -1.9633211368002392 2314 6 -1.0420571168936448 2314 7 -0.39257657044770156 2314 8 -5.051167945822765 2314 9 -3.612911688988401 2314 10-11 -0.5724360104886586 2314 12-13 -0.46384643314213037 2314 14-15 -1.551771511564496 2314 16-17 -1.0675235038509854 2314 18-19 -4.029830081065384 2314 20-21 -3.3454087981767344 2314 22-23 -5.5514549803570326 2314 24-25 -1.1598864235711375 2314 26-27 -4.411820051979703 2314 28-29 -3.1536622458399677 2314 30-31 -4.640655478672237 2314 32-33 -2.2452364800919824 2314 34-35 -2.571073676662685 2314 36-37 -7.12233588686043 2314 38-39 -8.42856629281441 2314 40-41 -5.805825093900474 2314 42-43 -4.612077005182705 2314 44-45 -5.228662220387143 2314 46-47 -7.9866662565931925 2314 48-49 -6.951861543041531 2314 50-51 -2.2587249729444636 2314 52-53 -3.882467175101091 2314 54-55 -5.5167320057861815 2314 56-57 -2.896852282026309 2314 58-59 -2.5659874361118398 2314 60-61 -0.605580757090685 2314 62-63 0.353825407959917 2314 64-65 -1.1982964385910506 2314 66-67 -0.004208093534764146 2314 68-69 0.12640957247975848 2314 70-71 1.0868825062600145 2314 72-73 -0.050879893050503 2314 74-75 -0.1558907244552863 2314 76-77 -1.4567430845737839 2314 78-79 -3.221978773463203 2314 80-81 -1.8777811929329395 2314 82-83 -2.354630304615956 2314 84-85 -0.6115087876975522 2314 86-87 0.4640265920915354 2314 88-89 0.2533414905116942 2314 90-91 0.2844999058154727 2314 92-93 0.14332001154659224 2314 94-95 0.3954765977622472 2314 96-97 0.6148390633642187 2314 98-99 0.291316088516659 2314 100-101 0.25450714640084726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 15.0 4 53.0 5 125.0 6 267.0 7 740.0 8 1745.0 9 3147.0 10 4748.0 11 6710.0 12 8747.0 13 10766.0 14 13347.0 15 16111.0 16 18321.0 17 20568.0 18 19209.0 19 16627.0 20 16760.0 21 17629.0 22 19411.0 23 22645.0 24 26971.0 25 32969.0 26 41600.0 27 52611.0 28 65893.0 29 82242.0 30 101811.0 31 123118.0 32 147480.0 33 178617.0 34 216346.0 35 265897.0 36 332707.0 37 407244.0 38 360823.0 39 79791.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.7692085418256 21.50991918380292 15.388697451801253 23.33217482257023 2 14.70066780815028 22.05928667394085 40.211326877769196 23.02871864013967 3 17.91353956219257 26.65436126593456 31.30067427304309 24.131424898829778 4 12.543967202369108 18.342846148443808 39.10941176126321 30.003774887923868 5 12.103820819177544 38.66980947113682 36.28699159870425 12.93937811098138 6 29.47994216255424 39.39256050673971 18.088467296713926 13.03903003399212 7 25.700536492421545 33.39889365584086 23.250565407486608 17.650004444250985 8 24.558601747194178 36.76269784689757 20.722499871061853 17.956200534846403 9 25.030679963085284 16.38809321148892 21.34879176727195 37.23243505815385 10-11 23.448051095352408 27.34265979825661 29.55650032975245 19.65278877663853 12-13 24.375527869111504 24.87077837306762 29.38366834694713 21.370025410873748 14-15 21.582306979595582 26.544230904992155 27.004604097709144 24.868858017703115 16-17 21.065310737273805 29.635856729053224 27.667071831166744 21.631760702506227 18-19 21.016113061745095 28.60281527754256 29.64247738278608 20.738594277926264 20-21 21.71618318214955 27.73967955304369 29.682530508960014 20.86160675584675 22-23 21.591268637963267 27.62312312696768 29.405194616128277 21.380413618940775 24-25 21.498649180865254 27.761148762421378 29.48601556007546 21.254186496637907 26-27 21.441512000162422 27.854804408976204 29.38524877265766 21.318434818203716 28-29 21.404010434137525 27.815605773673152 29.44914967485685 21.33123411733247 30-31 21.652162525874992 27.721753138560484 29.32447563242163 21.301608703142897 32-33 21.563389544823732 27.790907448199277 29.21476442310858 21.430938583868418 34-35 21.702743988312058 27.741208256180688 29.351994580354223 21.20405317515303 36-37 21.645559873664673 27.657955279313622 29.251814895226868 21.444669951794832 38-39 21.52800601203321 27.762948266556638 29.335711663823606 21.373334057586543 40-41 21.70146207852145 27.652233775273988 29.290055813975606 21.356248332228954 42-43 21.600278437159876 27.7574268046245 29.05602596795961 21.586268790256018 44-45 21.693393902305647 27.852960638337176 28.92531163892133 21.528333820435847 46-47 21.706632701132634 27.794943699456248 28.796950127270126 21.701473472140986 48-49 21.683437629760792 27.90454727545781 28.727004329780364 21.685010765001035 50-51 21.759218665251534 27.95648870179102 28.552513821979964 21.731778810977477 52-53 21.763610246535915 28.030891335670216 28.43338365325562 21.772114764538244 54-55 21.73856803704831 27.942041755462398 28.517976013442308 21.801414194046977 56-57 21.765259510811912 28.019475198951216 28.398612791696355 21.816652498540513 58-59 21.67046596796589 28.065619151990095 28.50503834172935 21.758876538314663 60-61 21.82704305085254 28.00512991780207 28.42314286477231 21.744684166573084 62-63 21.7406890350886 28.05604841209761 28.382043979772327 21.821218573041463 64-65 21.656353629561075 27.946916057426208 28.38730682489542 22.009423488117296 66-67 21.6547362745315 27.955307532324724 28.35814917084125 22.031807022302523 68-69 21.686632182272493 27.93797418023357 28.344386946068052 22.03100669142588 70-71 21.798136819775888 27.74215874160894 28.263153447344326 22.196550991270847 72-73 21.753592243877154 27.82979855048236 28.21997873240915 22.196630473231334 74-75 21.75214446647086 27.95327887876332 28.107878354921112 22.186698299844707 76-77 21.63446748911524 27.889613584264644 28.09907863178521 22.376840294834903 78-79 21.656213227480908 27.90649442237357 27.9993664656207 22.437925884524827 80-81 21.66069068945494 27.989172827821445 27.949613413256884 22.400523069466736 82-83 21.717885549892003 27.878947493557632 28.071697291919286 22.33146966463108 84-85 21.735339818893962 27.92226983892419 27.86422021838655 22.478170123795298 86-87 21.601722718292613 27.895025237588882 27.957136152759198 22.546115891359303 88-89 21.653894270162706 27.775097301884582 27.932060605173824 22.63894782277888 90-91 21.676572804350826 27.811781798921437 27.893882824333797 22.617762572393936 92-93 21.736833085537963 27.691987762706837 27.918451210953936 22.652727940801263 94-95 21.559781187302132 27.649116903116305 27.9518955902767 22.83920631930486 96-97 21.552457282816594 27.57736059693041 27.9727183575708 22.897463762682193 98-99 21.525513440197468 27.607675577050962 27.878304815177625 22.988506167573945 100-101 21.54967143472983 27.54677178609321 27.82034908082382 23.083207698353142 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 329.0 1 242.0 2 173.5 3 196.5 4 169.0 5 168.0 6 299.0 7 615.5 8 1053.0 9 1344.5 10 1328.0 11 1284.0 12 1396.0 13 1573.0 14 1777.5 15 1964.5 16 2159.5 17 2391.0 18 2682.0 19 3053.0 20 3569.5 21 4246.0 22 5195.0 23 6764.5 24 9007.0 25 12093.0 26 16243.0 27 21034.5 28 27092.5 29 34738.0 30 42991.0 31 50868.0 32 58191.5 33 65573.0 34 72639.0 35 78133.0 36 84554.5 37 90790.5 38 93809.5 39 95928.0 40 97633.5 41 98887.0 42 99259.5 43 99094.5 44 99454.5 45 100946.0 46 100632.0 47 98656.5 48 97597.0 49 95037.5 50 91857.5 51 88124.0 52 83707.5 53 79541.5 54 74940.0 55 68909.5 56 62364.0 57 55898.0 58 49267.0 59 42407.5 60 34971.0 61 28161.5 62 22287.0 63 17232.0 64 12626.5 65 9100.0 66 6739.0 67 4972.0 68 3836.0 69 3021.5 70 2335.0 71 1737.0 72 1276.5 73 973.0 74 763.5 75 596.5 76 432.0 77 315.0 78 223.0 79 146.5 80 106.0 81 77.5 82 58.0 83 45.5 84 37.0 85 28.0 86 20.0 87 14.5 88 10.5 89 8.0 90 5.0 91 2.5 92 0.5 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12261011774887531 2 0.0 3 0.00402360171610271 4 4.755165664485021E-4 5 1.8289098709557773E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.01929499913858345 26-27 0.007992336136076746 28-29 0.0054867296128673325 30-31 0.0019020662657940083 32-33 9.876113303161197E-4 34-35 0.004060179913521826 36-37 0.0012253696135403709 38-39 6.766966522536376E-4 40-41 0.002030089956760913 42-43 0.0014082606006359485 44-45 5.852511587058488E-4 46-47 0.03301182317075178 48-49 0.017338065576660767 50-51 0.08926909080135148 52-53 0.0010241895277352353 54-55 0.036761088406211125 56-57 0.001207080514830813 58-59 0.001207080514830813 60-61 0.0037309761367497855 62-63 0.0032737486690108415 64-65 0.01002242609283766 66-67 0.006017113475444508 68-69 0.0026336302141763196 70-71 0.005706198797382026 72-73 0.004828322059323252 74-75 0.0011156350212830242 76-77 0.0 78-79 0.0 80-81 2.3775828322425104E-4 82-83 5.120947638676176E-4 84-85 3.657819741911555E-5 86-87 0.0018289098709557774 88-89 0.0039870235186835945 90-91 0.0015362842916028531 92-93 0.0034566396561064193 94-95 8.778767380587732E-4 96-97 5.852511587058488E-4 98-99 0.002011800858051355 100-101 0.0032737486690108415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2733869.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.62163312701432 #Duplication Level Percentage of deduplicated Percentage of total 1 82.79400435081135 56.81459791677804 2 10.733809169651991 14.731430297904824 3 3.009935063604931 6.196399790125023 4 1.1673910963853191 3.2043313412758554 5 0.620003233919024 2.1272817227776852 6 0.3770185000809537 1.5522975116791458 7 0.24922235469118778 1.1971431493468527 8 0.17981682550540906 0.9871459383917225 9 0.13215912893689846 0.8162077734264266 >10 0.684261829840635 8.336421175419535 >50 0.03798385719368539 1.7515885722145832 >100 0.013858315651618566 1.5227328470664274 >500 3.2176423620131864E-4 0.13571832235798048 >1k 1.0725474535207338E-4 0.17531509963874914 >5k 1.0725474535207338E-4 0.4513885415971656 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6939 0.25381611189124276 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5327 0.19485205765162852 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3539 0.1294502406662499 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 3.657819741911555E-5 0.0 0.0 0.0 4 0.0 3.657819741911555E-5 0.0 0.0 0.0 5 0.0 3.657819741911555E-5 0.0 0.0 0.0 6 0.0 3.657819741911555E-5 0.0 0.0 0.0 7 0.0 3.657819741911555E-5 0.0 3.657819741911555E-5 0.0 8 0.0 3.657819741911555E-5 0.0 3.657819741911555E-5 0.0 9 0.0 3.657819741911555E-5 0.0 7.31563948382311E-5 0.0 10-11 0.0 3.657819741911555E-5 0.0 1.0973459225734664E-4 3.657819741911555E-5 12-13 0.0 5.486729612867332E-5 0.0 1.463127896764622E-4 3.657819741911555E-5 14-15 1.8289098709557774E-5 9.144549354778888E-5 0.0 1.463127896764622E-4 1.0973459225734664E-4 16-17 3.657819741911555E-5 1.0973459225734664E-4 0.0 1.463127896764622E-4 1.0973459225734664E-4 18-19 3.657819741911555E-5 1.0973459225734664E-4 0.0 1.463127896764622E-4 1.463127896764622E-4 20-21 3.657819741911555E-5 1.0973459225734664E-4 0.0 2.011800858051355E-4 2.194691845146933E-4 22-23 3.657819741911555E-5 2.194691845146933E-4 0.0 3.1091467806248216E-4 2.560473819338088E-4 24-25 7.31563948382311E-5 2.743364806433666E-4 0.0 7.132748496727531E-4 3.1091467806248216E-4 26-27 7.31563948382311E-5 3.1091467806248216E-4 0.0 0.001207080514830813 3.2920377677203995E-4 28-29 7.31563948382311E-5 3.2920377677203995E-4 0.0 0.0028896775961101285 3.2920377677203995E-4 30-31 7.31563948382311E-5 4.02360171610271E-4 0.0 0.009162838453488444 3.2920377677203995E-4 32-33 7.31563948382311E-5 4.206492703198288E-4 0.0 0.022861373386947218 3.2920377677203995E-4 34-35 7.31563948382311E-5 5.303838625771754E-4 0.0 0.04720416376936862 3.2920377677203995E-4 36-37 7.31563948382311E-5 6.218293561249643E-4 0.0 0.08562956015814949 3.2920377677203995E-4 38-39 7.31563948382311E-5 7.681421458014265E-4 0.0 0.13819242984941854 3.2920377677203995E-4 40-41 7.31563948382311E-5 9.144549354778886E-4 0.0 0.19315117147163963 3.2920377677203995E-4 42-43 7.31563948382311E-5 9.510331328970042E-4 0.0 0.25222496030351127 3.2920377677203995E-4 44-45 9.144549354778888E-5 0.0010241895277352353 0.0 0.32997191891784133 3.2920377677203995E-4 46-47 1.0973459225734664E-4 0.0010973459225734664 0.0 0.41668053589985476 3.2920377677203995E-4 48-49 1.0973459225734664E-4 0.0011156350212830242 0.0 0.5048888589760518 3.2920377677203995E-4 50-51 1.463127896764622E-4 0.0012436587122499287 0.0 0.5952735847986864 3.2920377677203995E-4 52-53 1.463127896764622E-4 0.0012436587122499287 0.0 0.686152116286479 3.474928754815977E-4 54-55 1.463127896764622E-4 0.0012436587122499287 0.0 0.7848583820219623 3.6578197419115545E-4 56-57 1.463127896764622E-4 0.0012436587122499287 0.0 0.9036277890418305 4.2064927031982874E-4 58-59 1.463127896764622E-4 0.0012802369096690442 0.0 1.0295299445584263 5.120947638676176E-4 60-61 1.463127896764622E-4 0.0013533933045072753 0.0 1.1609005405891797 5.852511587058488E-4 62-63 1.463127896764622E-4 0.0013899715019263907 0.0 1.293514795332183 5.852511587058488E-4 64-65 1.463127896764622E-4 0.001609440686441084 0.0 1.4380718315325276 6.035402574154065E-4 66-67 1.463127896764622E-4 0.001609440686441084 0.0 1.6014483503050072 6.949857509631955E-4 68-69 1.463127896764622E-4 0.001609440686441084 0.0 1.782766474911563 7.315639483823109E-4 70-71 1.463127896764622E-4 0.0016277297851506418 0.0 1.9756798880999784 7.315639483823109E-4 72-73 1.8289098709557773E-4 0.0016460188838601996 0.0 2.1833708930457165 7.315639483823109E-4 74-75 1.8289098709557773E-4 0.0016460188838601996 0.0 2.394445381252723 7.315639483823109E-4 76-77 1.8289098709557773E-4 0.0016460188838601996 0.0 2.6244490866241215 7.315639483823109E-4 78-79 1.8289098709557773E-4 0.0016460188838601996 0.0 2.877643369159239 7.315639483823109E-4 80-81 1.8289098709557773E-4 0.0016460188838601996 0.0 3.1501692290303596 7.681421458014265E-4 82-83 1.8289098709557773E-4 0.0016643079825697574 0.0 3.4327723822904463 8.230094419300998E-4 84-85 1.8289098709557773E-4 0.0017557534761175463 0.0 3.719051644391154 8.412985406396576E-4 86-87 1.8289098709557773E-4 0.0017557534761175463 0.0 4.0231079104375524 8.595876393492154E-4 88-89 1.8289098709557773E-4 0.0019020662657940083 0.0 4.3515618341625 8.778767380587732E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3440 0.0 53.49466 1 GTATCAA 6520 0.0 43.46669 1 ATCAACG 8025 0.0 35.15014 3 TCAACGC 8175 0.0 34.38838 4 CAACGCA 8170 0.0 34.351295 5 AACGCAG 8620 0.0 32.778374 6 TATCAAC 8695 0.0 32.55085 2 ACGCAGA 9995 0.0 27.936516 7 CGCAGAG 10130 0.0 27.470459 8 GCAGAGT 11405 0.0 23.858166 9 GTGGTAT 1750 0.0 23.367826 1 GTACATG 7310 0.0 22.376852 1 TGGTATC 1720 0.0 21.811438 2 GAGTACT 6875 0.0 21.688757 12-13 AGAGTAC 10185 0.0 21.190956 10-11 TACATGG 7725 0.0 20.778008 2 ACATGGG 7300 0.0 20.68664 3 CCGTCGA 215 1.082677E-6 19.878414 9 CAGAGTA 11155 0.0 19.837826 10-11 GTACTTT 7775 0.0 18.903315 14-15 >>END_MODULE