FastQCFastQC Report
Sat 18 Jun 2016
SRR1931122_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931122_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399401
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51430.2143451636470936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38880.16204044259379738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.051.2544981
GTATCAA52200.042.8883361
ATCAACG60750.035.0519333
CAACGCA61650.034.783395
TCAACGC62200.034.7813844
AACGCAG64500.033.3704346
TATCAAC65550.032.905712
CGCAGAG73450.028.404878
ACGCAGA73850.028.18777
GTACATG52550.024.7370431
GCAGAGT81350.023.9483859
TACATGG54200.022.4743522
GTGGTAT15450.021.8135471
GTACTAG7950.021.801791
GAGTACT54250.021.76814712-13
ACATGGG53500.021.7345543
TGGTATC15600.020.6783622
CAGAGTA81500.020.60126110-11
AGAGTAC73700.020.32569710-11
GTACTTT63200.019.23142814-15
Produced by FastQC (version 0.11.5)