##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931122_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2399401 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.88219893215015 31.0 28.0 33.0 23.0 34.0 2 28.628892794493293 31.0 28.0 33.0 18.0 34.0 3 28.66430704996789 31.0 28.0 34.0 16.0 34.0 4 31.936232001236977 35.0 32.0 37.0 19.0 37.0 5 31.998024923720546 35.0 32.0 37.0 19.0 37.0 6 32.267674723816484 35.0 32.0 37.0 23.0 37.0 7 32.00445361154721 35.0 32.0 37.0 19.0 37.0 8 32.174099702384055 35.0 32.0 37.0 23.0 37.0 9 33.16276937452306 37.0 32.0 39.0 19.0 39.0 10-11 33.19416033418341 37.0 32.0 39.0 20.5 39.0 12-13 33.156974178138626 37.0 32.0 39.0 20.5 39.0 14-15 34.01595231476523 38.0 32.0 40.0 19.0 41.0 16-17 34.03525379876061 38.0 32.0 40.0 20.5 41.0 18-19 34.02531944431131 38.0 32.0 40.0 20.0 41.0 20-21 34.222198165292085 38.0 32.0 40.0 23.0 41.0 22-23 34.101834374495965 38.0 32.0 40.0 22.5 41.0 24-25 34.01972659009478 38.0 32.0 40.0 21.0 41.0 26-27 33.687471164678186 38.0 32.0 40.0 18.5 41.0 28-29 33.59142615177704 38.0 32.0 40.0 18.0 41.0 30-31 33.33554103711718 37.5 31.5 40.0 17.5 41.0 32-33 33.167052110089145 37.0 31.0 40.0 17.5 41.0 34-35 33.10414265893863 37.0 31.0 40.0 17.5 41.0 36-37 32.77155673436829 37.0 30.5 40.0 15.5 41.0 38-39 32.6208782942076 37.0 30.0 40.0 15.5 41.0 40-41 32.520161490305284 37.0 30.0 40.0 15.0 41.0 42-43 32.73409884383644 37.0 30.0 40.0 15.0 41.0 44-45 32.739967391861555 37.0 30.5 40.0 13.5 41.0 46-47 32.510325702123154 37.0 30.0 40.0 9.0 41.0 48-49 32.295867176849555 37.0 30.0 40.0 8.5 41.0 50-51 31.80232045414668 36.5 30.0 39.5 8.0 40.5 52-53 31.74709333704537 36.5 30.0 39.5 7.5 40.5 54-55 31.70901529173323 36.5 30.0 40.0 6.5 41.0 56-57 31.491029427761344 36.0 29.5 40.0 2.0 41.0 58-59 31.314602477868434 36.0 29.0 40.0 2.0 41.0 60-61 31.139010736429633 36.0 29.0 40.0 2.0 41.0 62-63 31.018656948129973 35.5 29.0 39.0 2.0 41.0 64-65 30.695628200538387 35.0 29.0 39.0 2.0 41.0 66-67 30.463382319170492 35.0 29.0 39.0 2.0 40.0 68-69 30.285920110894345 35.0 29.0 39.0 2.0 40.0 70-71 29.997034051415334 34.5 29.0 38.0 2.0 40.0 72-73 29.64882693638954 34.0 28.5 37.5 2.0 40.0 74-75 29.222756012854873 34.0 28.0 37.0 2.0 39.5 76-77 28.920756263750828 34.0 28.0 36.5 2.0 39.0 78-79 28.507844249460597 34.0 27.5 36.0 2.0 39.0 80-81 28.107854418665326 34.0 27.0 36.0 2.0 38.5 82-83 27.75991966328263 33.0 26.0 35.0 2.0 37.0 84-85 27.42993793034178 33.0 26.0 35.0 2.0 37.0 86-87 27.10073159926165 33.0 26.0 35.0 2.0 36.0 88-89 26.750996186131456 33.0 25.5 35.0 2.0 36.0 90-91 26.370543106383636 33.0 25.0 35.0 2.0 36.0 92-93 26.00724097389307 32.0 24.0 35.0 2.0 35.0 94-95 25.712561385112366 32.0 23.5 35.0 2.0 35.0 96-97 25.329773347598007 32.0 20.5 35.0 2.0 35.0 98-99 24.97275986798372 32.0 18.5 35.0 2.0 35.0 100-101 24.38494065810592 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 4.751319008407478 1101 2 5.566546437226801 1101 3 4.980518373633711 1101 4 6.427251806103882 1101 5 5.730262645256342 1101 6 5.287419712796023 1101 7 5.835558434671288 1101 8 5.045854118753287 1101 9 5.801836326668013 1101 10-11 5.82705006554534 1101 12-13 5.806446729525579 1101 14-15 5.956625536444939 1101 16-17 5.911301048344274 1101 18-19 5.843254463463946 1101 20-21 5.2685909124158385 1101 22-23 5.2527070037718175 1101 24-25 5.178301853355453 1101 26-27 5.3870606181220815 1101 28-29 5.237580946697612 1101 30-31 5.347083445333343 1101 32-33 5.155420407363248 1101 34-35 4.967090881032345 1101 36-37 5.118038618390873 1101 38-39 4.853279084042754 1101 40-41 5.201798079104439 1101 42-43 4.885355171136677 1101 44-45 4.914680983948799 1101 46-47 4.751996681737445 1101 48-49 4.8589814981894115 1101 50-51 4.726436467178587 1101 52-53 4.580738761249272 1101 54-55 4.7080803113809715 1101 56-57 4.6906718979291036 1101 58-59 4.571600392110188 1101 60-61 4.537976356663371 1101 62-63 4.380379493373354 1101 64-65 4.356827498869066 1101 66-67 4.326856787346056 1101 68-69 4.413458954785288 1101 70-71 4.192604088845268 1101 72-73 4.035555322841777 1101 74-75 4.196279497864261 1101 76-77 4.144847616938634 1101 78-79 4.067161995498559 1101 80-81 3.8328462816388793 1101 82-83 3.834727000828 1101 84-85 3.7186434836652147 1101 86-87 3.563429643005364 1101 88-89 3.713715130670302 1101 90-91 3.597475644952688 1101 92-93 3.5635698215225418 1101 94-95 3.564146101420725 1101 96-97 3.586858277192704 1101 98-99 3.2954514750435813 1101 100-101 3.4282729431013124 1102 1 5.109068488728454 1102 2 5.863983023211226 1102 3 5.454300763116791 1102 4 6.796601138324334 1102 5 6.2268630561171285 1102 6 5.647528358600354 1102 7 6.192994237916135 1102 8 5.789092866532702 1102 9 6.600890509878294 1102 10-11 6.611800118777442 1102 12-13 6.6818827823171 1102 14-15 6.938881227242646 1102 16-17 6.7514386942627205 1102 18-19 6.710355259978964 1102 20-21 6.039109926884699 1102 22-23 6.255658019192367 1102 24-25 5.970547413100473 1102 26-27 6.132023524209405 1102 28-29 6.086315892280744 1102 30-31 6.320558648278922 1102 32-33 6.087110927243767 1102 34-35 6.060212430996227 1102 36-37 6.191294324882126 1102 38-39 5.963086016674254 1102 40-41 6.266459924947743 1102 42-43 6.171766947569974 1102 44-45 6.184153810775996 1102 46-47 6.104065729831177 1102 48-49 5.851276356965943 1102 50-51 5.944013465794647 1102 52-53 5.710936883488003 1102 54-55 5.964658762908943 1102 56-57 5.931042263563423 1102 58-59 5.966763807404494 1102 60-61 5.851863164382035 1102 62-63 5.6598409685391005 1102 64-65 5.752853090191042 1102 66-67 5.744740478443692 1102 68-69 5.807541307901982 1102 70-71 5.685628745723427 1102 72-73 5.596104395285682 1102 74-75 5.535138313924136 1102 76-77 5.495524977300509 1102 78-79 5.400679485756456 1102 80-81 5.342680755466411 1102 82-83 5.230838180073622 1102 84-85 5.198215966416367 1102 86-87 5.1626603398044 1102 88-89 5.269796678185209 1102 90-91 5.234124587605194 1102 92-93 5.278885400229619 1102 94-95 5.179608375988334 1102 96-97 5.081306105758781 1102 98-99 4.7938098531374145 1102 100-101 4.7394677272657155 1103 1 4.608094140365935 1103 2 5.4669879443475615 1103 3 4.940637965395446 1103 4 6.517212956222025 1103 5 5.983977133887297 1103 6 5.239645051260034 1103 7 5.803908323012667 1103 8 5.121797613161203 1103 9 5.7338124365257706 1103 10-11 5.870702288005607 1103 12-13 5.97857556085739 1103 14-15 6.045740431225077 1103 16-17 5.921333979823462 1103 18-19 5.830928555994593 1103 20-21 5.300774633358479 1103 22-23 5.513696076551582 1103 24-25 5.190285522757481 1103 26-27 5.3647498602386925 1103 28-29 5.151837921263535 1103 30-31 5.431178753155091 1103 32-33 5.167590825719028 1103 34-35 5.1272609650603265 1103 36-37 5.201829095118686 1103 38-39 4.967404042966557 1103 40-41 5.232812415172109 1103 42-43 5.032643913705787 1103 44-45 4.913039747035043 1103 46-47 4.801331523802823 1103 48-49 4.787514117451426 1103 50-51 4.695973609968192 1103 52-53 4.3791185103623675 1103 54-55 4.768574296911471 1103 56-57 4.901829592767495 1103 58-59 4.949366316139628 1103 60-61 4.714652779958204 1103 62-63 4.653228435151522 1103 64-65 4.656661592985582 1103 66-67 4.659252150749321 1103 68-69 4.629282419031274 1103 70-71 4.556868478092802 1103 72-73 4.411927467808141 1103 74-75 4.521020346164832 1103 76-77 4.509890404797922 1103 78-79 4.512244126916453 1103 80-81 4.553678372838316 1103 82-83 4.455526507456231 1103 84-85 4.387378525641083 1103 86-87 4.376532776842463 1103 88-89 4.4145487391156415 1103 90-91 4.457225226947632 1103 92-93 4.474630096212671 1103 94-95 4.368196175872516 1103 96-97 4.189494577720232 1103 98-99 4.051091112368443 1103 100-101 4.1018789924903345 1104 1 4.3817520126305105 1104 2 5.403608812020938 1104 3 4.92524931701486 1104 4 6.40726175913565 1104 5 5.833318563077935 1104 6 5.366425121632517 1104 7 5.962054158901108 1104 8 5.4043742150341 1104 9 6.136086439766348 1104 10-11 6.209293069118846 1104 12-13 6.131929899414704 1104 14-15 6.075144396171382 1104 16-17 5.942457389529505 1104 18-19 6.118802519318333 1104 20-21 5.54015496242868 1104 22-23 5.725840871859681 1104 24-25 5.385649817291995 1104 26-27 5.513099214903665 1104 28-29 5.442107133340066 1104 30-31 5.662265719695785 1104 32-33 5.688073585298493 1104 34-35 5.459883501775817 1104 36-37 5.513252092553802 1104 38-39 5.29644591870786 1104 40-41 5.651101338476156 1104 42-43 5.659251347532571 1104 44-45 5.547347265951743 1104 46-47 5.425496479022065 1104 48-49 5.22701652998288 1104 50-51 5.23141871135622 1104 52-53 5.068802176983581 1104 54-55 5.338945352171162 1104 56-57 5.2584080925949515 1104 58-59 5.257839066417869 1104 60-61 5.0706809472341625 1104 62-63 5.008131560625245 1104 64-65 5.049665785016078 1104 66-67 5.076626519335679 1104 68-69 5.077765121858743 1104 70-71 4.97529523449111 1104 72-73 4.824819285159627 1104 74-75 4.878568914833615 1104 76-77 4.775965980945017 1104 78-79 4.917594117280039 1104 80-81 4.851148185724298 1104 82-83 4.480632841284461 1104 84-85 4.437449932229949 1104 86-87 4.50251670040932 1104 88-89 4.546337745071934 1104 90-91 4.53565659016121 1104 92-93 4.481954961055298 1104 94-95 4.362598581090957 1104 96-97 4.281003135465202 1104 98-99 4.117564371510273 1104 100-101 4.136706963457687 1105 1 4.1694598184444125 1105 2 5.0610832344372305 1105 3 4.668792360523305 1105 4 6.1038769907417745 1105 5 3.6044999356065937 1105 6 4.596770185356178 1105 7 5.220838184460593 1105 8 0.7828494047333976 1105 9 0.4711817151900881 1105 10-11 1.9831862203614214 1105 12-13 5.2949166835138435 1105 14-15 3.3510466064567694 1105 16-17 5.4176707660079195 1105 18-19 5.876740575309217 1105 20-21 3.221405010643487 1105 22-23 5.143860539940057 1105 24-25 5.180345743571113 1105 26-27 5.33056950123137 1105 28-29 3.0027108005137038 1105 30-31 5.1018363379193765 1105 32-33 5.343795510781259 1105 34-35 5.308231618015935 1105 36-37 5.434481056804817 1105 38-39 5.261720606617125 1105 40-41 5.570028424040974 1105 42-43 2.7194942492746534 1105 44-45 4.745950045379544 1105 46-47 5.084795379368437 1105 48-49 4.932235537950628 1105 50-51 4.942782629591029 1105 52-53 4.818059431605825 1105 54-55 5.0007462003956995 1105 56-57 4.9483117045218385 1105 58-59 4.9189524160146725 1105 60-61 4.766555332384119 1105 62-63 4.573536324663472 1105 64-65 4.638352840957715 1105 66-67 4.768643927699362 1105 68-69 4.714571266294282 1105 70-71 4.615550769995899 1105 72-73 4.5186955597365674 1105 74-75 4.665999774801794 1105 76-77 4.59620513231696 1105 78-79 4.657167020143671 1105 80-81 4.6400869377886345 1105 82-83 4.485345994342882 1105 84-85 4.370728414421922 1105 86-87 4.413135825991535 1105 88-89 4.430327165705023 1105 90-91 4.394760020266361 1105 92-93 4.325118546622782 1105 94-95 4.14935743051063 1105 96-97 4.1156839119105015 1105 98-99 4.128039060369009 1105 100-101 4.232456286818518 1106 1 3.7561207785886594 1106 2 4.223085201352291 1106 3 4.182223257475243 1106 4 5.684039921569742 1106 5 5.5557593740625 1106 6 4.632241902513655 1106 7 5.330310804131255 1106 8 4.997157351797757 1106 9 5.607496269542256 1106 10-11 5.512283946414922 1106 12-13 5.878848475277767 1106 14-15 5.3759248376358215 1106 16-17 5.3946841093735465 1106 18-19 5.640753075477168 1106 20-21 4.93684113194729 1106 22-23 5.066130888187573 1106 24-25 4.940562281191067 1106 26-27 5.29082733030376 1106 28-29 5.1510858671617115 1106 30-31 5.203455651045896 1106 32-33 5.111879479243758 1106 34-35 4.667465451838805 1106 36-37 4.775403507852321 1106 38-39 4.658344145563326 1106 40-41 4.933881755491068 1106 42-43 1.8389967265979443 1106 44-45 4.033356676721262 1106 46-47 4.224994443494396 1106 48-49 4.044304111910993 1106 50-51 4.264930184924033 1106 52-53 4.172000028935841 1106 54-55 4.338501629812061 1106 56-57 4.219473786908775 1106 58-59 4.123440534726942 1106 60-61 4.050591663618906 1106 62-63 3.9905688849399823 1106 64-65 4.10538866194813 1106 66-67 4.225327293372207 1106 68-69 4.1619110972418305 1106 70-71 4.062092102903648 1106 72-73 3.826728385785838 1106 74-75 3.94628762865889 1106 76-77 3.876006369684152 1106 78-79 4.005208346988784 1106 80-81 3.8977586766405175 1106 82-83 3.988813689843983 1106 84-85 4.03406783114411 1106 86-87 4.058685049826927 1106 88-89 4.1930644809544475 1106 90-91 4.161472162149032 1106 92-93 4.127962708421045 1106 94-95 4.207424977428044 1106 96-97 4.222533082427489 1106 98-99 4.116141649310304 1106 100-101 4.2766023550038135 1107 1 3.0535000678547597 1107 2 0.2630148917653905 1107 3 1.4283871327813173 1107 4 2.9147228724726126 1107 5 2.8511491354620517 1107 6 3.9635610901990717 1107 7 4.890483605915048 1107 8 4.394197592544618 1107 9 5.049266818483179 1107 10-11 2.664662573106721 1107 12-13 5.035762539616815 1107 14-15 3.6657986867984107 1107 16-17 2.284219060431038 1107 18-19 2.856564956118728 1107 20-21 2.6334858135814656 1107 22-23 4.3199161729012445 1107 24-25 4.236824595032889 1107 26-27 3.0898114632662548 1107 28-29 3.9536134564998093 1107 30-31 3.670867374113609 1107 32-33 3.980010497783656 1107 34-35 4.462656140120391 1107 36-37 4.741537688398335 1107 38-39 4.598305061952466 1107 40-41 3.746026223461957 1107 42-43 4.046558466715094 1107 44-45 4.4593435927966425 1107 46-47 4.457937893342034 1107 48-49 2.632376405761267 1107 50-51 3.8590521355969187 1107 52-53 3.970859318159828 1107 54-55 4.5253595400391795 1107 56-57 3.4959761948135792 1107 58-59 2.954694141846101 1107 60-61 3.618440475144123 1107 62-63 4.250479108754721 1107 64-65 4.443660969004764 1107 66-67 4.542595769531037 1107 68-69 4.330830692008082 1107 70-71 4.13269153785788 1107 72-73 4.202902048540185 1107 74-75 4.430356289105127 1107 76-77 4.187495332820802 1107 78-79 4.227489615118387 1107 80-81 4.305528108606019 1107 82-83 4.080259547295597 1107 84-85 4.0928708865366765 1107 86-87 3.996021540252844 1107 88-89 4.3491023077047295 1107 90-91 4.4488860632135285 1107 92-93 4.114069179673667 1107 94-95 4.329776804832026 1107 96-97 4.343019699253929 1107 98-99 4.304598537082253 1107 100-101 4.214788610129361 1108 1 -1.240842235610824 1108 2 -0.511579038118203 1108 3 -1.037607591262006 1108 4 -2.643475858581848 1108 5 -7.889690742522021 1108 6 -6.337629897923737 1108 7 -4.730698932629867 1108 8 -5.0762590618751915 1108 9 -7.177257060376025 1108 10-11 -2.9183522664644634 1108 12-13 -1.299444663648341 1108 14-15 -5.98596528832563 1108 16-17 -5.396573112644482 1108 18-19 -3.51947621545548 1108 20-21 -4.088312714280761 1108 22-23 -2.922983918384375 1108 24-25 -7.643587095561681 1108 26-27 -8.127355513972692 1108 28-29 -5.262579556001036 1108 30-31 -5.229004624840254 1108 32-33 -1.9715989456255478 1108 34-35 -0.4719080402076834 1108 36-37 -3.8431089759044603 1108 38-39 -4.961886235485252 1108 40-41 -2.5363542690780214 1108 42-43 -3.36687359591237 1108 44-45 -1.1878374922196961 1108 46-47 -1.9843839299561665 1108 48-49 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-5.684543491215887 2313 60-61 -4.718096782213426 2313 62-63 -6.743619200575274 2313 64-65 -6.9880625751405105 2313 66-67 -8.137836290581674 2313 68-69 -9.25410199427871 2313 70-71 -10.116423991259214 2313 72-73 -7.2680199927873055 2313 74-75 -7.2120356958666925 2313 76-77 -6.109592957723969 2313 78-79 -6.873614016691253 2313 80-81 -7.379114972170438 2313 82-83 -8.134009394470215 2313 84-85 -7.4008438911715295 2313 86-87 -6.205997871143527 2313 88-89 -9.349089614649683 2313 90-91 -8.601027837850967 2313 92-93 -7.965212285573671 2313 94-95 -7.8522285176476245 2313 96-97 -8.106127138566986 2313 98-99 -8.185774036493203 2313 100-101 -8.489320657246967 2314 1 -13.800414686906691 2314 2 -14.548576718279843 2314 3 -13.989150408493666 2314 4 -25.301307183883054 2314 5 -20.25416478777134 2314 6 -18.89896841971276 2314 7 -19.33524631470475 2314 8 -15.148959802726797 2314 9 -17.35032947425514 2314 10-11 -15.896418568941451 2314 12-13 -17.19350662578259 2314 14-15 -17.375044586886332 2314 16-17 -18.468584138112814 2314 18-19 -17.99810626359836 2314 20-21 -17.164030492224835 2314 22-23 -18.124476846619054 2314 24-25 -18.89058627239323 2314 26-27 -17.23623076653574 2314 28-29 -18.813294215882795 2314 30-31 -20.03567478373671 2314 32-33 -16.494534164762968 2314 34-35 -17.983164245151684 2314 36-37 -17.565439290953478 2314 38-39 -16.545543847298966 2314 40-41 -20.171931360050607 2314 42-43 -20.111828571092595 2314 44-45 -16.24381294683318 2314 46-47 -15.006414911023295 2314 48-49 -15.238476163302623 2314 50-51 -14.971163291621039 2314 52-53 -15.002942491911075 2314 54-55 -15.713486977305061 2314 56-57 -14.283528855416472 2314 58-59 -13.571422373203466 2314 60-61 -14.026481875381126 2314 62-63 -16.05743907635167 2314 64-65 -14.618419717997654 2314 66-67 -15.762370452072359 2314 68-69 -18.031819385583056 2314 70-71 -18.27550784219089 2314 72-73 -17.29092371949538 2314 74-75 -15.428184764189675 2314 76-77 -15.806176808655644 2314 78-79 -15.54434383035585 2314 80-81 -15.280512487698388 2314 82-83 -15.252410015588229 2314 84-85 -15.506356313531775 2314 86-87 -15.447457498472719 2314 88-89 -14.652428123966454 2314 90-91 -14.20188187511805 2314 92-93 -14.027557006070566 2314 94-95 -13.739728517647624 2314 96-97 -13.297198567138414 2314 98-99 -13.393072173139167 2314 100-101 -13.2221622721538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76540.0 3 12243.0 4 8540.0 5 6907.0 6 7239.0 7 8079.0 8 9577.0 9 10610.0 10 12032.0 11 13620.0 12 14999.0 13 16193.0 14 17725.0 15 19732.0 16 21196.0 17 22819.0 18 21342.0 19 19150.0 20 18977.0 21 20663.0 22 24292.0 23 28851.0 24 35684.0 25 42869.0 26 49956.0 27 57708.0 28 64928.0 29 72584.0 30 82227.0 31 92948.0 32 107777.0 33 128968.0 34 157312.0 35 193016.0 36 240696.0 37 298716.0 38 284316.0 39 78260.0 40 110.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.107701249782146 20.126729426794725 14.395093602319605 23.37047572110353 2 14.53087012793593 20.647042412470135 41.416713689081504 23.405373770512437 3 17.84745442716745 25.795563142634347 30.761052446006314 25.59592998419189 4 11.513984583923234 17.62437208326779 40.24229458339888 30.619348749410097 5 12.47279670011417 37.63748129249993 36.03629506121998 13.853426946165925 6 29.398155294039125 38.315026719452014 18.802860086853862 13.483957899655005 7 25.902943907960424 32.57582142132988 23.362476751045733 18.158757919663966 8 24.37490638563652 36.18485639817406 21.309195047899262 18.13104216829015 9 24.914367773411914 16.57393492701756 21.818507241503323 36.6931900580672 10-11 23.358976745622666 26.962605919425066 29.789753515203856 19.88866381974842 12-13 24.332096112358645 24.53347112283604 29.46283205503888 21.671600709766437 14-15 21.61059617351591 26.236291881772683 27.217450743834554 24.93566120087685 16-17 21.041326583410946 29.316595395230987 27.841176529498362 21.8009014918597 18-19 20.927370325335865 28.273084636112294 29.841682438412914 20.957862600138927 20-21 21.752141771070335 27.57558544732554 29.84824397614175 20.824028805462376 22-23 21.412050846679822 27.41327737472935 29.64649709429664 21.528174684294182 24-25 21.323897502986938 27.594797722585252 29.621034381486993 21.460270392940814 26-27 21.20228724659534 27.749112940110145 29.61443438054086 21.434165432753655 28-29 21.169954554036057 27.746216052428185 29.68688492272517 21.396944470810592 30-31 21.38117435122875 27.57272328378183 29.66209041871427 21.384011946275145 32-33 21.26325973402621 27.737153496785833 29.482672987992526 21.51691378119543 34-35 21.526895184431574 27.82192309698962 29.478666457974644 21.172515260604165 36-37 21.414775551392413 27.56227565676931 29.419287769060592 21.603661022777683 38-39 21.344363490227973 27.82989793759408 29.526131973781016 21.29960659839693 40-41 21.529294086110667 27.622188636579352 29.414264302320852 21.434252974989125 42-43 21.39348244613642 27.711901231003576 29.29818131221798 21.596435010642022 44-45 21.50512604873751 27.837598366071965 29.183582460636888 21.473693124553638 46-47 21.49385226668335 27.837562181272098 29.00042757772007 21.66815797432448 48-49 21.445799015105894 27.96395910826806 28.99507552947601 21.595166347150037 50-51 21.373552587974057 28.120936396146618 28.823210564832983 21.68230045104634 52-53 21.436644705981486 28.192188637050506 28.75345944927883 21.61770720768918 54-55 21.364078608415763 28.09096086822483 28.76252880410076 21.782431719258643 56-57 21.398792146464313 28.178684890876543 28.595009498221707 21.827513464437434 58-59 21.379561672946373 28.210360020464716 28.662536507914606 21.747541798674312 60-61 21.56856033369894 28.169529413115235 28.589220322073324 21.6726899311125 62-63 21.40951012356834 28.190014924558255 28.63871024476526 21.76176470710815 64-65 21.34197243395331 28.170989342756798 28.682054396076357 21.80498382721354 66-67 21.376481046727914 28.177032517699207 28.55283464498014 21.893651790592735 68-69 21.43824646234623 28.139523155987682 28.527286601947736 21.894943779718353 70-71 21.566986927153902 28.003635073920535 28.451080082070483 21.978297916855084 72-73 21.51176481130082 28.03168374106704 28.478941202408436 21.9776102452237 74-75 21.401820704417478 28.22825363496973 28.432512948023277 21.937412712589516 76-77 21.529623435182362 28.171114373962503 28.29162361772792 22.00763857312721 78-79 21.528935763550987 28.172843972308087 28.250425835448095 22.04779442869283 80-81 21.604996413688248 28.24604974324842 28.162007934480314 21.986945908583017 82-83 21.687079400233642 28.181846219118857 28.264220945144224 21.866853435503277 84-85 21.707021877543603 28.19393256900368 28.165779709185752 21.933265844266963 86-87 21.577406194295992 28.1657171935829 28.2493630701996 22.007513541921504 88-89 21.68855893616782 28.056377404193793 28.244195113697128 22.01086854594126 90-91 21.697061058155764 28.153339104218094 28.188931320775477 21.960668516850664 92-93 21.7921264515602 28.076653298052307 28.17538627349076 21.955833976896734 94-95 21.63289921109477 28.075507178666676 28.196495708720636 22.09509790151792 96-97 21.655342312518833 28.01470033562543 28.232608888635124 22.097348463220612 98-99 21.71683682719145 28.01349170063695 28.018993073687977 22.250678398483622 100-101 21.78833383832048 28.01857630300229 27.999571559735116 22.19351829894211 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 147.0 2 122.0 3 163.0 4 163.5 5 188.5 6 254.5 7 452.5 8 799.5 9 1110.5 10 1154.5 11 1145.0 12 1303.5 13 1453.5 14 1639.0 15 1882.0 16 2048.0 17 2289.5 18 2638.0 19 2955.0 20 3429.0 21 4179.0 22 5307.5 23 6982.5 24 9000.5 25 11904.5 26 15818.5 27 20473.0 28 25832.5 29 32021.5 30 38604.0 31 45223.0 32 51821.0 33 58287.0 34 64964.5 35 70664.5 36 76353.5 37 81579.0 38 84380.5 39 85924.0 40 86778.5 41 87589.5 42 87900.5 43 87887.0 44 87969.5 45 87910.0 46 86859.5 47 85090.5 48 83820.0 49 81983.5 50 78887.0 51 75242.0 52 71385.5 53 67396.5 54 63268.0 55 57779.5 56 52184.0 57 46704.5 58 41028.0 59 35765.5 60 29785.0 61 23921.5 62 18856.0 63 14353.5 64 10868.5 65 8186.5 66 6062.5 67 4487.0 68 3479.0 69 2728.5 70 2075.0 71 1557.5 72 1147.5 73 860.5 74 668.0 75 548.5 76 428.5 77 291.5 78 200.0 79 151.0 80 119.0 81 86.5 82 67.5 83 58.5 84 45.0 85 34.5 86 33.0 87 27.5 88 21.5 89 17.5 90 11.5 91 7.5 92 6.0 93 4.0 94 1.5 95 2.5 96 2.5 97 1.5 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04430272388817042 2 0.6579142044201866 3 0.0 4 0.647078166592412 5 0.41614552965510976 6 0.09464862271875356 7 0.10498453572370771 8 0.10836037827774517 9 0.10515124399798116 10-11 0.1497873844346985 12-13 0.3061805842374826 14-15 0.12928226669906365 16-17 0.12207213383673675 18-19 0.22305567097788154 20-21 0.1039634475437828 22-23 0.09964987094695718 24-25 0.09573222650153101 26-27 0.21905467239531867 28-29 0.10829786267489261 30-31 0.12526042958221656 32-33 0.10150450049824936 34-35 0.14695334377204977 36-37 0.13503370216149782 38-39 0.1301158080704309 40-41 0.1060056239036326 42-43 0.1056722073550857 44-45 0.09306489411315574 46-47 0.09068930120475903 48-49 0.09546132555583664 50-51 0.08233304895680214 52-53 0.09012666077908611 54-55 0.2985536806894721 56-57 0.3183086111908764 58-59 0.33206204381843635 60-61 0.15956065701397973 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2399401.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.18021855178372 #Duplication Level Percentage of deduplicated Percentage of total 1 87.56620889204699 67.58379140039334 2 8.240850557858542 12.720612942162221 3 1.9511774140504479 4.5177689774915315 4 0.7809891219602461 2.411076444778298 5 0.40339310761816755 1.556698410412669 6 0.24234554515839532 1.122256928422567 7 0.1664278862025285 0.8991458441155776 8 0.1078083309045987 0.6656536432735958 9 0.08858732391655282 0.6153470118717482 >10 0.4284318047824989 5.77343010717642 >50 0.018236393102619625 0.9692694175919306 >100 0.005270083110116607 0.6286241448142448 >500 1.0941571533032871E-4 0.06059092929919883 >1k 1.0941571532993046E-4 0.25857759653465506 >5k 5.470785766496523E-5 0.2171562016619417 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5143 0.2143451636470936 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3888 0.16204044259379738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.167706856836352E-5 0.0 10-11 0.0 0.0 0.0 4.167706856836352E-5 0.0 12-13 0.0 0.0 0.0 1.458697399892723E-4 8.335413713672704E-5 14-15 0.0 8.335413713672704E-5 0.0 1.6670827427345408E-4 1.6670827427345408E-4 16-17 0.0 8.335413713672704E-5 0.0 1.6670827427345408E-4 1.6670827427345408E-4 18-19 0.0 1.041926714209088E-4 0.0 1.6670827427345408E-4 1.8754680855763585E-4 20-21 0.0 1.2503120570509055E-4 0.0 1.8754680855763585E-4 2.083853428418176E-4 22-23 0.0 1.6670827427345408E-4 0.0 3.3341654854690815E-4 2.083853428418176E-4 24-25 0.0 1.6670827427345408E-4 0.0 5.834789599570893E-4 2.083853428418176E-4 26-27 0.0 1.6670827427345408E-4 0.0 0.0011669579199141786 2.083853428418176E-4 28-29 0.0 1.6670827427345408E-4 0.0 0.0024797855798176296 2.083853428418176E-4 30-31 0.0 1.6670827427345408E-4 0.0 0.0076060650137263425 2.083853428418176E-4 32-33 0.0 2.083853428418176E-4 0.0 0.01975493050140431 2.083853428418176E-4 34-35 0.0 2.9173947997854465E-4 0.0 0.04140616762266916 2.500624114101811E-4 36-37 0.0 3.750936171152717E-4 0.0 0.07435189032596051 2.500624114101811E-4 38-39 0.0 4.167706856836352E-4 0.0 0.12050924376542313 2.500624114101811E-4 40-41 0.0 4.584477542519987E-4 0.0 0.16914638278470334 2.917394799785446E-4 42-43 0.0 4.7928628853618045E-4 0.0 0.21886712558676102 3.3341654854690815E-4 44-45 4.167706856836352E-5 5.20963357104544E-4 0.0 0.28711332536745626 3.3341654854690815E-4 46-47 4.167706856836352E-5 5.418018913887258E-4 0.0 0.362402949736205 3.3341654854690815E-4 48-49 4.167706856836352E-5 5.418018913887258E-4 0.0 0.44175608829036916 3.3341654854690815E-4 50-51 4.167706856836352E-5 5.418018913887258E-4 0.0 0.5252560951670855 3.3341654854690815E-4 52-53 4.167706856836352E-5 5.418018913887258E-4 0.0 0.6081517845495605 3.3341654854690815E-4 54-55 4.167706856836352E-5 5.418018913887258E-4 0.0 0.7019251888283784 3.3341654854690815E-4 56-57 4.167706856836352E-5 5.418018913887258E-4 0.0 0.8141198574144131 3.542550828310899E-4 58-59 4.167706856836352E-5 5.834789599570893E-4 0.0 0.9338789139456056 3.750936171152717E-4 60-61 4.167706856836352E-5 6.04317494241271E-4 0.0 1.0567637506194254 3.750936171152717E-4 62-63 4.167706856836352E-5 6.251560285254528E-4 0.0 1.1811489617617064 4.3760921996781696E-4 64-65 4.167706856836352E-5 6.251560285254528E-4 0.0 1.3177247154602336 4.584477542519987E-4 66-67 4.167706856836352E-5 6.251560285254528E-4 0.0 1.4725758637259885 4.584477542519987E-4 68-69 4.167706856836352E-5 6.251560285254528E-4 0.0 1.6426599805534798 4.584477542519987E-4 70-71 4.167706856836352E-5 6.251560285254528E-4 0.0 1.8265183685428155 4.584477542519987E-4 72-73 4.167706856836352E-5 6.251560285254528E-4 0.0 2.0110644281635293 4.584477542519987E-4 74-75 4.167706856836352E-5 6.251560285254528E-4 0.0 2.204904474074988 4.7928628853618045E-4 76-77 8.335413713672704E-5 6.251560285254528E-4 0.0 2.4172908154993684 5.418018913887258E-4 78-79 8.335413713672704E-5 6.251560285254528E-4 0.0 2.6472648798595984 5.418018913887258E-4 80-81 8.335413713672704E-5 6.251560285254528E-4 0.0 2.904995871886358 5.626404256729076E-4 82-83 1.041926714209088E-4 6.251560285254528E-4 0.0 3.170812215215381 5.834789599570893E-4 84-85 1.2503120570509055E-4 6.668330970938163E-4 0.0 3.4361701107901514 5.834789599570893E-4 86-87 1.2503120570509055E-4 6.668330970938163E-4 0.0 3.7110928936013616 5.834789599570893E-4 88-89 1.2503120570509055E-4 7.501872342305434E-4 0.0 4.014647822519037 6.04317494241271E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2865 0.0 51.254498 1 GTATCAA 5220 0.0 42.888336 1 ATCAACG 6075 0.0 35.051933 3 CAACGCA 6165 0.0 34.78339 5 TCAACGC 6220 0.0 34.781384 4 AACGCAG 6450 0.0 33.370434 6 TATCAAC 6555 0.0 32.90571 2 CGCAGAG 7345 0.0 28.40487 8 ACGCAGA 7385 0.0 28.1877 7 GTACATG 5255 0.0 24.737043 1 GCAGAGT 8135 0.0 23.948385 9 TACATGG 5420 0.0 22.474352 2 GTGGTAT 1545 0.0 21.813547 1 GTACTAG 795 0.0 21.80179 1 GAGTACT 5425 0.0 21.768147 12-13 ACATGGG 5350 0.0 21.734554 3 TGGTATC 1560 0.0 20.678362 2 CAGAGTA 8150 0.0 20.601261 10-11 AGAGTAC 7370 0.0 20.325697 10-11 GTACTTT 6320 0.0 19.231428 14-15 >>END_MODULE