FastQCFastQC Report
Sat 18 Jun 2016
SRR1931122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2399401
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64770.2699423731172905No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54530.22726505490328627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35160.14653657308636614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33250.052.5061151
GTATCAA59400.039.6418761
ATCAACG71250.032.3931473
TCAACGC71800.032.144344
CAACGCA71900.031.9675395
TATCAAC76050.030.4735032
AACGCAG76300.030.248546
ACGCAGA88100.025.9275747
CGCAGAG89100.025.1035968
GCAGAGT99300.022.285869
GTACATG57650.021.7016071
GAGTACT65000.021.29697612-13
ACATGGG56000.021.116073
TACATGG58100.021.0884822
AGAGTAC88450.020.26804410-11
TACGGGC2351.1359407E-720.208074
CAGAGTA99550.019.67774810-11
TGGTATC17350.019.4339312
GTGGTAT17650.019.405431
GCGTCGG2502.1798041E-718.9955869
Produced by FastQC (version 0.11.5)