##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931122_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2399401 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.294847338981686 31.0 31.0 34.0 30.0 34.0 2 31.2966256994975 31.0 31.0 34.0 28.0 34.0 3 31.460163599164957 31.0 31.0 34.0 28.0 34.0 4 35.110836829692076 35.0 35.0 37.0 32.0 37.0 5 34.986832130185824 35.0 35.0 37.0 32.0 37.0 6 35.084686553018855 36.0 35.0 37.0 32.0 37.0 7 34.94227100847253 36.0 35.0 37.0 32.0 37.0 8 34.99226598638577 36.0 35.0 37.0 32.0 37.0 9 36.39965641424672 38.0 35.0 39.0 32.0 39.0 10-11 36.293247981475375 38.0 35.0 39.0 32.0 39.0 12-13 36.235501068808425 38.0 35.0 39.0 32.0 39.0 14-15 37.15731051208198 39.0 36.0 41.0 31.5 41.0 16-17 37.230001571225486 39.0 36.0 40.5 32.0 41.0 18-19 37.28932450224035 39.0 36.0 40.5 31.5 41.0 20-21 37.26507636697659 39.0 36.0 40.0 31.0 41.0 22-23 37.14965255911788 39.0 36.0 40.0 31.0 41.0 24-25 37.032491442655896 39.0 36.0 40.0 31.0 41.0 26-27 36.77733067544774 39.0 36.0 40.0 30.5 41.0 28-29 36.512982823629734 38.5 35.0 40.0 30.0 41.0 30-31 36.308906889677885 38.0 35.0 40.0 30.0 41.0 32-33 36.14156866651302 38.0 35.0 40.0 30.0 41.0 34-35 35.896260983470455 38.0 34.5 40.0 29.0 41.0 36-37 36.15774707937523 38.0 35.0 40.0 30.0 41.0 38-39 36.151212948565075 38.0 35.0 40.0 30.0 41.0 40-41 36.01249603546886 38.0 35.0 40.0 29.0 41.0 42-43 35.849806680917446 38.0 35.0 40.0 29.0 41.0 44-45 35.64592058601293 38.0 34.5 40.0 28.0 41.0 46-47 35.4353171895819 38.0 34.0 40.0 27.0 41.0 48-49 35.1845129263512 38.0 34.0 40.0 27.0 41.0 50-51 34.85860908618443 38.0 33.5 40.0 26.0 41.0 52-53 34.72477339135892 38.0 33.0 40.0 25.5 41.0 54-55 34.51168625002657 38.0 33.0 40.0 24.5 41.0 56-57 34.3005266731155 38.0 33.0 40.0 24.0 41.0 58-59 34.064808675165175 37.0 33.0 40.0 24.0 41.0 60-61 33.786708849416996 37.0 32.0 40.0 23.0 41.0 62-63 33.52800761523397 37.0 32.0 40.0 22.5 41.0 64-65 33.30275327050376 36.0 32.0 39.0 22.0 41.0 66-67 33.07815533960351 36.0 31.5 39.0 22.0 41.0 68-69 32.75913384215477 36.0 31.0 39.0 22.0 40.0 70-71 31.912104937857407 35.0 30.0 38.5 18.5 40.0 72-73 31.904042300557514 35.0 31.0 38.0 20.0 40.0 74-75 31.60358564491721 35.0 30.5 37.0 19.5 39.5 76-77 30.87901376218481 34.0 30.0 36.0 18.0 39.0 78-79 30.890995502627533 34.0 30.0 36.0 18.0 39.0 80-81 30.614550673272205 34.0 30.0 36.0 17.5 38.5 82-83 30.253924833739752 34.0 30.0 36.0 13.5 37.0 84-85 29.887009507789653 34.0 29.0 35.0 9.5 37.0 86-87 29.55281922446477 34.0 29.0 35.0 7.0 36.5 88-89 29.238727707456988 34.0 29.0 35.0 4.5 36.0 90-91 28.918070801837626 34.0 29.0 35.0 2.0 36.0 92-93 28.582389938155398 34.0 29.0 35.0 2.0 35.5 94-95 28.198545595338167 33.5 29.0 35.0 2.0 35.0 96-97 27.834795434360494 33.0 27.5 35.0 2.0 35.0 98-99 27.36428258552864 33.0 27.0 35.0 2.0 35.0 100-101 26.69417012829452 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7598425136977234 1101 2 0.6276228123468002 1101 3 0.4315427792744124 1101 4 0.6776572379844552 1101 5 0.6546628067157201 1101 6 0.7228728557484487 1101 7 0.6277290232275874 1101 8 0.6433807392301887 1101 9 0.9815325374795165 1101 10-11 0.7764853588542664 1101 12-13 0.8753423412488957 1101 14-15 1.2870891235536206 1101 16-17 0.9020020701246594 1101 18-19 0.7211505135741447 1101 20-21 0.7750219266617435 1101 22-23 0.6275330596521442 1101 24-25 0.5372643527612411 1101 26-27 0.7835696973500887 1101 28-29 0.6799041267999044 1101 30-31 0.8484220970832652 1101 32-33 0.9953607778181848 1101 34-35 0.7061525089997431 1101 36-37 0.8951171286800275 1101 38-39 0.6951953524291028 1101 40-41 0.7838159556938251 1101 42-43 0.8285097920202418 1101 44-45 0.7446954754832191 1101 46-47 0.754741350835836 1101 48-49 0.5685119098547347 1101 50-51 0.48077786799964173 1101 52-53 0.303286725718813 1101 54-55 0.3894744981915821 1101 56-57 0.10836021047211375 1101 58-59 0.1624950692204905 1101 60-61 0.45359601386414283 1101 62-63 0.34236496983557174 1101 64-65 0.286544432691187 1101 66-67 -0.03693327622979581 1101 68-69 -0.08470728272387618 1101 70-71 0.20862734345213596 1101 72-73 -0.2743025421058505 1101 74-75 -0.5971735172028865 1101 76-77 -0.5894400862078726 1101 78-79 -0.4378384878827468 1101 80-81 -0.5467435342509752 1101 82-83 -0.635965944826669 1101 84-85 -0.5907978670672129 1101 86-87 -0.5950304800985826 1101 88-89 -0.5630929219898242 1101 90-91 -0.45512231039610285 1101 92-93 -0.6871121955675683 1101 94-95 -0.7694325269274209 1101 96-97 -0.8547809005955749 1101 98-99 -0.9457211865779733 1101 100-101 -1.0565053009427814 1102 1 0.875134145223452 1102 2 0.813344441088379 1102 3 0.5921682075773091 1102 4 0.7537136603336876 1102 5 0.7853670456574378 1102 6 0.6865839636175224 1102 7 0.8734902655670567 1102 8 0.8299380006026311 1102 9 1.2983194534836144 1102 10-11 1.041735896551799 1102 12-13 1.2204496085403207 1102 14-15 1.6169802917517657 1102 16-17 1.3778102826516303 1102 18-19 1.1698525899071441 1102 20-21 1.221387796580892 1102 22-23 0.8655596627231645 1102 24-25 0.66687546874833 1102 26-27 0.9497110663189972 1102 28-29 1.0656549532779707 1102 30-31 1.2116427139789323 1102 32-33 1.341108767833994 1102 34-35 1.2939829621807775 1102 36-37 1.466455995052236 1102 38-39 1.1950946522065422 1102 40-41 1.2950241471798236 1102 42-43 1.598444948633066 1102 44-45 1.5008249173608377 1102 46-47 1.4748596375118908 1102 48-49 1.1290865085214818 1102 50-51 1.083386744847779 1102 52-53 1.1266012829894834 1102 54-55 0.9818334098658141 1102 56-57 1.017491797714925 1102 58-59 1.1006155332300622 1102 60-61 1.4625716538070321 1102 62-63 1.341640306040631 1102 64-65 1.2406145235335373 1102 66-67 0.9429645568796587 1102 68-69 0.9471864491401547 1102 70-71 1.3574055043977324 1102 72-73 0.6768430282759006 1102 74-75 0.6637498171359972 1102 76-77 0.6434499820861568 1102 78-79 0.6290243771107527 1102 80-81 0.4903449050534796 1102 82-83 0.49850033624387535 1102 84-85 0.44888930640783187 1102 86-87 0.44467359783902793 1102 88-89 0.5562431385322206 1102 90-91 0.47007965676022323 1102 92-93 0.3176089447367545 1102 94-95 0.2801054435954562 1102 96-97 0.3722125133483125 1102 98-99 0.3289176246343466 1102 100-101 0.09694038070811573 1103 1 0.5777872058912266 1103 2 0.4198392265076585 1103 3 0.2863810400988953 1103 4 0.5427289030741562 1103 5 0.6370139584120977 1103 6 0.5082490831736948 1103 7 0.5610121375005974 1103 8 0.47957622225952434 1103 9 0.8116521749873584 1103 10-11 0.706796072135198 1103 12-13 0.8200965302580983 1103 14-15 1.1559218811213654 1103 16-17 0.8229156751695399 1103 18-19 0.7307348949380881 1103 20-21 0.7155498901136568 1103 22-23 0.5039231218439824 1103 24-25 0.3143709228934597 1103 26-27 0.7093438263251386 1103 28-29 0.5697830006374787 1103 30-31 0.8394425650354478 1103 32-33 0.8862902396076038 1103 34-35 0.7564381076676128 1103 36-37 1.0992491744383344 1103 38-39 0.8539721339159598 1103 40-41 0.8810524047437198 1103 42-43 1.111783223367155 1103 44-45 1.07265341879922 1103 46-47 1.0863251973515489 1103 48-49 0.9323402338622202 1103 50-51 0.7677617375408872 1103 52-53 0.28719076715868397 1103 54-55 0.4270874243642311 1103 56-57 0.5299989389743018 1103 58-59 0.6001915392221022 1103 60-61 0.7767675324137997 1103 62-63 0.7230224290815102 1103 64-65 0.697075005577318 1103 66-67 0.462570187894201 1103 68-69 0.3438973390053732 1103 70-71 0.7423923071945353 1103 72-73 0.21409643562113345 1103 74-75 -0.002529633495594652 1103 76-77 0.19157744255689835 1103 78-79 0.12057991097298881 1103 80-81 0.11061321181314554 1103 82-83 0.142003797172908 1103 84-85 0.15904059199040077 1103 86-87 0.2383915498544411 1103 88-89 0.032438388552289865 1103 90-91 0.004186574565398615 1103 92-93 -0.39569004819953335 1103 94-95 -0.4976916893009431 1103 96-97 -0.6716355011672483 1103 98-99 -0.9196968931075418 1103 100-101 -1.0009181572763666 1104 1 0.3972142353847481 1104 2 0.31623844523445044 1104 3 0.11450188606177036 1104 4 0.5876633487975269 1104 5 0.2807751612193812 1104 6 0.3715295089127508 1104 7 0.38316659588462443 1104 8 0.3155043727003246 1104 9 0.8146544130730575 1104 10-11 0.5520178867671603 1104 12-13 0.6575005660491797 1104 14-15 1.0083198246420721 1104 16-17 0.7298291070355205 1104 18-19 0.6329254841609995 1104 20-21 0.6208551739917496 1104 22-23 0.2910372653572253 1104 24-25 0.2864337972567128 1104 26-27 0.009949520645903931 1104 28-29 0.5194424981422898 1104 30-31 0.7527038417346006 1104 32-33 0.8985204866730712 1104 34-35 0.6353372844969556 1104 36-37 0.8826543460312521 1104 38-39 0.3632854137322852 1104 40-41 0.7909201782902713 1104 42-43 0.9183567811997335 1104 44-45 1.031689423183984 1104 46-47 0.9384106715811882 1104 48-49 0.7696539520782437 1104 50-51 0.5967750468377844 1104 52-53 0.3539785428534401 1104 54-55 0.7411051119448331 1104 56-57 0.5365799812153114 1104 58-59 0.5354449312017096 1104 60-61 0.9672642476535884 1104 62-63 -0.03924219293872966 1104 64-65 -1.0513467459799344 1104 66-67 -0.3973652976477524 1104 68-69 0.2728105038703248 1104 70-71 0.9570300484952909 1104 72-73 0.6116091743509067 1104 74-75 0.27510930607228445 1104 76-77 0.03830147127874639 1104 78-79 0.026398295803371497 1104 80-81 0.07819186856560378 1104 82-83 -0.06501589234411753 1104 84-85 -0.2712446191994182 1104 86-87 0.1279235857887464 1104 88-89 0.18185012928476496 1104 90-91 0.38075539608149356 1104 92-93 0.3889755225313607 1104 94-95 0.3428717476308236 1104 96-97 0.2662881868140481 1104 98-99 0.18111894550042607 1104 100-101 -0.2834742865307689 1105 1 -0.441731756318152 1105 2 -1.7703033633815508 1105 3 -0.4326802033097863 1105 4 0.2847975794108706 1105 5 0.5195182650185899 1105 6 0.03718816470176023 1105 7 -0.14616729209205914 1105 8 -0.13078908070814776 1105 9 0.5257288051066098 1105 10-11 0.8481248304706597 1105 12-13 0.9157442594041996 1105 14-15 1.2821279101315213 1105 16-17 0.7635329773344282 1105 18-19 0.5741463245880922 1105 20-21 0.9511651076659717 1105 22-23 0.26272529390276844 1105 24-25 -0.1746897590436447 1105 26-27 0.9415217327768062 1105 28-29 0.4667449316696519 1105 30-31 0.9687527852517661 1105 32-33 0.9929986303822673 1105 34-35 0.5085198830168665 1105 36-37 0.7238551587696875 1105 38-39 0.6676539718633165 1105 40-41 1.0013476611450898 1105 42-43 1.2677534582056325 1105 44-45 1.1773897899725512 1105 46-47 1.4112749336988273 1105 48-49 1.168124370737047 1105 50-51 0.7792552698661197 1105 52-53 0.9142535779093137 1105 54-55 0.3887285819035924 1105 56-57 0.3233509362601197 1105 58-59 -0.26284595035483704 1105 60-61 0.42536035919623316 1105 62-63 0.9405589187611838 1105 64-65 1.173506310126669 1105 66-67 0.9055986222486183 1105 68-69 0.7468588029409844 1105 70-71 0.29900336627328983 1105 72-73 -0.3287094409554676 1105 74-75 -0.34452791682609885 1105 76-77 -0.09594949613279269 1105 78-79 -0.5969735124506492 1105 80-81 -0.4763697564840186 1105 82-83 -0.4351220895340724 1105 84-85 -0.3786982424901666 1105 86-87 -0.6821148258345531 1105 88-89 -0.9498276510183139 1105 90-91 -1.0810247717001005 1105 92-93 -0.7474384945957198 1105 94-95 -1.0443251295926181 1105 96-97 -1.2129454143661285 1105 98-99 -1.7411817226232635 1105 100-101 -1.132251915098145 1106 1 0.24742592754596515 1106 2 0.27614748881176254 1106 3 -0.3335914007979568 1106 4 0.28517044778060807 1106 5 0.08135958852106029 1106 6 0.31045761862885257 1106 7 0.30720630149976813 1106 8 0.4452096399122425 1106 9 0.7865842132545922 1106 10-11 0.006591003759091052 1106 12-13 0.17964956565077728 1106 14-15 -0.11086830580179452 1106 16-17 -0.4313090320649451 1106 18-19 0.25651523487717043 1106 20-21 0.4005090815225856 1106 22-23 0.3013561676528411 1106 24-25 0.19153671670795092 1106 26-27 0.5284653099733134 1106 28-29 0.3803849731053006 1106 30-31 0.08941440540382928 1106 32-33 0.22692965202784166 1106 34-35 -1.306775585180688 1106 36-37 -1.0278969260390198 1106 38-39 -0.9912364219424461 1106 40-41 0.4322841349009323 1106 42-43 0.6351580839625228 1106 44-45 0.3705174831338027 1106 46-47 0.016663501013233883 1106 48-49 -0.1654436793789813 1106 50-51 0.3607612215702005 1106 52-53 -0.21394245424427538 1106 54-55 0.3852858578999303 1106 56-57 0.015284782109951323 1106 58-59 0.41373134216463114 1106 60-61 0.6349941466153837 1106 62-63 0.5188552137709195 1106 64-65 0.5331988067815701 1106 66-67 0.28175716700674514 1106 68-69 0.3050607109337946 1106 70-71 0.7027073406411333 1106 72-73 0.0571643599128997 1106 74-75 0.05268730241676778 1106 76-77 0.16420520262148486 1106 78-79 0.13532538260974647 1106 80-81 0.13979650256609943 1106 82-83 0.11151693370679183 1106 84-85 0.2355704776943739 1106 86-87 0.2866927589464652 1106 88-89 0.2364535260764633 1106 90-91 0.40918142011704717 1106 92-93 0.05133229819033147 1106 94-95 0.1315914669168059 1106 96-97 0.2701163402780935 1106 98-99 0.2775907480376816 1106 100-101 0.16743558070307074 1107 1 0.32893715648817334 1107 2 0.33453013667525866 1107 3 0.14128274392842854 1107 4 -0.908076679196455 1107 5 0.19485939320829715 1107 6 0.3555741903415779 1107 7 0.5508080222977298 1107 8 0.43688000551337325 1107 9 1.0150220826623269 1107 10-11 0.8563234395612014 1107 12-13 0.8754265080952592 1107 14-15 1.2339998118298823 1107 16-17 0.9018297155056771 1107 18-19 0.780492978998943 1107 20-21 0.1873747982437166 1107 22-23 0.377903053019665 1107 24-25 0.3059284020548034 1107 26-27 0.6382817731321495 1107 28-29 0.7536045020954418 1107 30-31 0.9950684128652796 1107 32-33 1.07694516724667 1107 34-35 0.8471796825557405 1107 36-37 0.9523670086655969 1107 38-39 0.7869813088466202 1107 40-41 0.12310041891652901 1107 42-43 0.08876035978866526 1107 44-45 -0.2327184364854773 1107 46-47 0.32533061962426046 1107 48-49 0.7557728521581311 1107 50-51 -0.9795414507927518 1107 52-53 0.30756308302677127 1107 54-55 0.8277504176956398 1107 56-57 0.7986930041380731 1107 58-59 0.879397734726588 1107 60-61 1.1545075237723808 1107 62-63 1.301008380191675 1107 64-65 1.1059789459778244 1107 66-67 0.9593885985086672 1107 68-69 1.0480810810699026 1107 70-71 1.4849350372696435 1107 72-73 0.8878060070921556 1107 74-75 0.6050032214524386 1107 76-77 0.8584345339508985 1107 78-79 0.8310231320080526 1107 80-81 0.8212765807393367 1107 82-83 1.0027568290726947 1107 84-85 1.0220569251544873 1107 86-87 1.0314634984621094 1107 88-89 1.1490697529647669 1107 90-91 1.381448436506627 1107 92-93 1.2404191955993156 1107 94-95 1.188686118475676 1107 96-97 1.3715865482752854 1107 98-99 1.4704264171271682 1107 100-101 1.227993292278235 1108 1 0.6726526571885323 1108 2 0.5673972343709863 1108 3 0.38640952531823203 1108 4 0.7144205734333511 1108 5 0.600533184176804 1108 6 0.5585149452208356 1108 7 0.8108493145801958 1108 8 0.7139552444779937 1108 9 1.1284217049460779 1108 10-11 0.8298410541648948 1108 12-13 0.9511615082084219 1108 14-15 1.2358563299499465 1108 16-17 0.9998235694436559 1108 18-19 0.7536679581893466 1108 20-21 0.6901590096038746 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1.1018067189218925 2311 68-69 1.4653217406399293 2311 70-71 1.8672836864500866 2311 72-73 1.2902651906496807 2311 74-75 1.3432451136344845 2311 76-77 1.3908365006279162 2311 78-79 1.3245984185260546 2311 80-81 1.2977579728433284 2311 82-83 1.3749159454675102 2311 84-85 1.6531629447387282 2311 86-87 1.5337914665541454 2311 88-89 1.6687152917884553 2311 90-91 1.7867310850256075 2311 92-93 1.916001516558083 2311 94-95 2.024545977487829 2311 96-97 2.083714828437646 2311 98-99 2.282870236394313 2311 100-101 2.3463706940326006 2312 1 -0.3425896592273894 2312 2 -0.18824086205617263 2312 3 -0.5160313261706264 2312 4 0.1847766089744738 2312 5 -1.2077694564374681 2312 6 -0.43386909872698 2312 7 0.026534854668007313 2312 8 -2.8960120859837453 2312 9 -6.266719481561616 2312 10-11 -1.0278029935284678 2312 12-13 -6.272120219739641 2312 14-15 -2.183910821557639 2312 16-17 -3.2845668419428193 2312 18-19 -2.879185736381679 2312 20-21 -3.867769075794712 2312 22-23 -2.245194226746044 2312 24-25 -2.938616367228711 2312 26-27 -3.5366489854252947 2312 28-29 -6.534770830322962 2312 30-31 -6.6721562949471895 2312 32-33 -1.3657317864110396 2312 34-35 0.4968412349267126 2312 36-37 0.8748479557782218 2312 38-39 0.9207657254394803 2312 40-41 -2.275700549652562 2312 42-43 -4.709085990719572 2312 44-45 -3.3548980846383714 2312 46-47 0.1434200208439549 2312 48-49 -1.829677739030231 2312 50-51 -7.660387337348176 2312 52-53 -1.9994401170580218 2312 54-55 0.5448235567744177 2312 56-57 0.8765635364293018 2312 58-59 1.1488018311379307 2312 60-61 1.4055157289238878 2312 62-63 1.460622207066983 2312 64-65 1.2685879181114643 2312 66-67 0.9171834924321871 2312 68-69 0.9394150048886374 2312 70-71 1.7879081204361569 2312 72-73 1.0680462675978823 2312 74-75 0.7044102486612793 2312 76-77 0.8125199683199575 2312 78-79 0.5261785562004313 2312 80-81 0.6124864593653889 2312 82-83 -0.1814281118192831 2312 84-85 0.13052238770679025 2312 86-87 0.4484402781104819 2312 88-89 0.5019669706538075 2312 90-91 0.8418280558922824 2312 92-93 0.6787853895532194 2312 94-95 0.7692792643028632 2312 96-97 1.1375374947282069 2312 98-99 1.3490227273504622 2312 100-101 1.2136302216243848 2313 1 -0.4313634070684955 2313 2 -0.20870718329969407 2313 3 -0.6069639686576735 2313 4 -1.2453183823899465 2313 5 -4.129185691325205 2313 6 -3.8713043318876004 2313 7 -3.121781207508157 2313 8 -4.425502586847305 2313 9 -3.9948714677446873 2313 10-11 -1.0113738052728607 2313 12-13 -4.146278250827724 2313 14-15 -3.68321997527093 2313 16-17 -6.385819000837465 2313 18-19 -3.5185207968307353 2313 20-21 -5.364660267504554 2313 22-23 -2.6368824823591694 2313 24-25 -1.0549591996984873 2313 26-27 -4.117823424112686 2313 28-29 -6.458238015124344 2313 30-31 -2.2672124262079905 2313 32-33 -7.185053893492221 2313 34-35 -1.6834480569558465 2313 36-37 -2.343351526087062 2313 38-39 -1.3477791795691587 2313 40-41 -0.8248801696871055 2313 42-43 -4.604876146159988 2313 44-45 -4.355761642496752 2313 46-47 -2.7298744523857508 2313 48-49 -0.6937105542288435 2313 50-51 1.294534942444571 2313 52-53 -0.2389694780252043 2313 54-55 -4.0861349924483825 2313 56-57 -4.440513320220099 2313 58-59 1.0183614166301567 2313 60-61 -10.134769245169384 2313 62-63 -3.152568639219716 2313 64-65 -3.0568654497641887 2313 66-67 -3.066041896168848 2313 68-69 -5.118011592693399 2313 70-71 -6.239963209442941 2313 72-73 -0.9562844750618815 2313 74-75 0.7169318376077349 2313 76-77 -1.3255197553415243 2313 78-79 1.189844359309241 2313 80-81 2.654002003406845 2313 82-83 3.3461193838629235 2313 84-85 2.348160556964128 2313 86-87 2.203168257385091 2313 88-89 -3.101055481850512 2313 90-91 0.8488012855986717 2313 92-93 2.275719759155983 2313 94-95 2.2618310777743638 2313 96-97 2.664135076766204 2313 98-99 2.819510637540443 2313 100-101 2.4676457656658357 2314 1 -28.831363407068494 2314 2 -14.89100531994566 2314 3 -4.871870800955808 2314 4 -3.074666208476902 2314 5 -1.194403082629556 2314 6 -0.9009627169807644 2314 7 -0.2421228224149914 2314 8 -3.9787634564125227 2314 9 -2.7315174304776093 2314 10-11 -0.8606750475088845 2314 12-13 0.07491740134619107 2314 14-15 -1.6496796026001235 2314 16-17 -1.4549183797194516 2314 18-19 -3.780477318569865 2314 20-21 -2.6795670998026964 2314 22-23 -5.0936371407442635 2314 24-25 -0.8941672742326432 2314 26-27 -3.785525287466726 2314 28-29 -2.737352921956642 2314 30-31 -4.35284907217072 2314 32-33 -2.3908768748586837 2314 34-35 -1.9855443302477767 2314 36-37 -6.15445401055911 2314 38-39 -7.2964593037927585 2314 40-41 -5.532799424345491 2314 42-43 -3.7844568915016055 2314 44-45 -5.591786487217249 2314 46-47 -8.260231595242896 2314 48-49 -6.630201237458664 2314 50-51 -2.9296886600399006 2314 52-53 -3.0491402854786216 2314 54-55 -5.423557352696829 2314 56-57 -2.302858040716991 2314 58-59 -2.6432845461027625 2314 60-61 -0.7144276302625485 2314 62-63 0.47608042910326276 2314 64-65 -1.9307008534908974 2314 66-67 -0.46068475331171044 2314 68-69 0.37111884208921 2314 70-71 1.325952939625374 2314 72-73 0.6223645932610999 2314 74-75 0.4979877382288542 2314 76-77 -1.359215407515439 2314 78-79 -3.2821276903802 2314 80-81 -2.524336505909929 2314 82-83 -2.7069862061991863 2314 84-85 -0.864494722538975 2314 86-87 0.0058856486894391935 2314 88-89 0.0036029032426583285 2314 90-91 0.28226401851792815 2314 92-93 0.33550236785163534 2314 94-95 0.3701385311905163 2314 96-97 0.5113400457103019 2314 98-99 1.1062342400249179 2314 100-101 1.2351923495167725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 17.0 4 48.0 5 82.0 6 190.0 7 566.0 8 1451.0 9 2577.0 10 3952.0 11 5777.0 12 7677.0 13 9435.0 14 11794.0 15 14170.0 16 16634.0 17 18419.0 18 17135.0 19 14649.0 20 14103.0 21 14917.0 22 16136.0 23 18667.0 24 22387.0 25 27452.0 26 34797.0 27 44243.0 28 55371.0 29 69754.0 30 85764.0 31 104557.0 32 126010.0 33 153669.0 34 187520.0 35 231866.0 36 291095.0 37 364118.0 38 334054.0 39 78270.0 40 71.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.032407604517026 21.398327193252673 15.34990946407239 23.21935573815791 2 14.705128488318541 21.87966913408805 40.4590562394531 22.95614613814031 3 18.005033201793214 26.396634306796905 31.281374152449832 24.31695833896005 4 12.48773648679625 18.238021767162877 39.21240113128565 30.061840614755226 5 12.103004256071111 38.45676161834062 36.39461764544077 13.045616480147505 6 29.55245913459234 39.40458472760493 18.024706999788698 13.01824913801403 7 25.689286617785022 33.29189243482019 23.202749352859318 17.816071594535472 8 24.35712079806585 37.00198507877591 20.709335371619833 17.931558751538404 9 24.680451496019217 16.62798340085713 21.48694611696836 37.204618986155296 10-11 23.40765466047568 27.41882244776925 29.5677962958255 19.605726595929568 12-13 24.240445844608715 24.87441657313638 29.513782814960898 21.371354767294005 14-15 21.403008500871675 26.65936206578225 27.14244096755815 24.79518846578792 16-17 20.896527925094638 29.759489972705687 27.791352925167573 21.552629177032102 18-19 20.83799248229037 28.62228947974932 29.845865697313624 20.693852340646686 20-21 21.505450735412712 27.825403090187926 29.950829394503042 20.718316779896316 22-23 21.29839905876508 27.722502407892634 29.711144573166386 21.267953960175895 24-25 21.243555172806193 27.859035596663134 29.78832580177631 21.10908342875436 26-27 21.22557587653002 27.911310085630962 29.690222597997117 21.172891439841894 28-29 21.153033418830276 27.93457774873234 29.820518933391572 21.091869899045808 30-31 21.48426616597916 27.751959631027763 29.660462601081328 21.103311601911752 32-33 21.304084171568967 27.91486151659696 29.554281617997948 21.22677269383613 34-35 21.48921826564901 27.89249003620829 29.618922087056188 20.99936961108651 36-37 21.45541166208146 27.715979483058774 29.56278759997883 21.265821254880944 38-39 21.256014285288582 27.90155337301145 29.65628993851149 21.186142403188473 40-41 21.52463097045367 27.751334466270798 29.544973831645216 21.179060731630315 42-43 21.42828861765238 27.768942169275256 29.412990511723436 21.38977870134893 44-45 21.497216794921382 27.894691655219322 29.277312752563983 21.330778797295316 46-47 21.516248602672487 27.908676739444772 29.07158256368718 21.503492094195558 48-49 21.455740022784102 28.003585599412435 29.03658170077127 21.504092677032197 50-51 21.569033132157593 28.10346621972379 28.817199681227834 21.510300966890785 52-53 21.614023344873164 28.11112331338574 28.735641285912283 21.53921205582881 54-55 21.582712216745197 28.029671007290798 28.783557910913597 21.60405886505041 56-57 21.612754464227105 28.13041871867289 28.650636549991365 21.60619026710864 58-59 21.546268696527765 28.153736638192594 28.732053667286827 21.567940997992814 60-61 21.635930367408097 28.147371689539586 28.695592374049596 21.52110556900272 62-63 21.570075984123815 28.116543166242725 28.66997149605492 21.643409353578537 64-65 21.465461870657126 28.11497968688586 28.632821340878106 21.78673710157891 66-67 21.490742176363792 28.10459273411724 28.592178947912444 21.812486141606524 68-69 21.53169722900876 28.009971834132298 28.610715866494285 21.847615070364657 70-71 21.593689278709213 27.874985099708997 28.534474063490663 21.99685155809113 72-73 21.52524680600016 27.924708149677603 28.536031394110832 22.014013650211407 74-75 21.419174657791874 28.096544087109155 28.415522256682507 22.068758998416467 76-77 21.418908302530507 28.07021419095849 28.324590178965497 22.1862873275455 78-79 21.42436799851296 28.0579611327994 28.278870434746008 22.238800433941638 80-81 21.443703238153667 28.10862085061549 28.206187234099307 22.241488677131542 82-83 21.491850944521907 28.05493983154458 28.25853337214878 22.194675851784737 84-85 21.45897338063128 28.07831709558996 28.18807372427976 22.274635799499 86-87 21.352950419089005 28.032638554021034 28.2205836446302 22.39382738225977 88-89 21.377253295622634 27.94661735267382 28.230343693356325 22.445785658347223 90-91 21.4083786784564 27.953642947166262 28.17768036356119 22.460298010816146 92-93 21.408210144762936 27.88447973012539 28.2056101759799 22.50169994913177 94-95 21.287264267522108 27.829675379045366 28.198145106471124 22.684915246961406 96-97 21.216796008487986 27.825554937574797 28.248953953533633 22.70869510040358 98-99 21.19312183535533 27.798797263104635 28.117778025065842 22.890302876474195 100-101 21.162905555080886 27.72634917591749 28.12887737643972 22.981867892561898 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 256.0 1 184.5 2 131.5 3 182.0 4 173.5 5 162.5 6 281.5 7 545.0 8 916.5 9 1262.5 10 1332.5 11 1300.0 12 1380.0 13 1534.5 14 1761.5 15 1986.5 16 2191.5 17 2439.5 18 2730.5 19 3010.0 20 3467.0 21 4251.5 22 5348.5 23 6861.0 24 9021.5 25 12071.0 26 16198.5 27 21002.0 28 26346.0 29 32756.5 30 39599.5 31 46058.0 32 52526.5 33 58973.0 34 65154.5 35 70632.0 36 76464.5 37 81611.0 38 84115.5 39 85594.5 40 86294.5 41 86856.0 42 86992.5 43 87126.5 44 87051.5 45 86898.0 46 86229.0 47 84517.5 48 83526.5 49 82164.0 50 79261.5 51 75311.5 52 71391.5 53 67561.5 54 63549.5 55 58189.5 56 52475.0 57 46975.5 58 41384.0 59 35878.0 60 29661.0 61 23754.5 62 18676.5 63 14155.5 64 10468.0 65 7719.0 66 5774.5 67 4343.0 68 3297.5 69 2503.0 70 1877.5 71 1444.0 72 1103.5 73 827.5 74 636.5 75 510.5 76 381.5 77 255.5 78 185.5 79 128.5 80 91.0 81 74.0 82 53.5 83 38.0 84 35.0 85 29.5 86 18.0 87 11.5 88 9.0 89 5.5 90 3.0 91 3.5 92 3.0 93 1.5 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15282981044018903 2 0.0 3 0.005543050119592348 4 2.9173947997854465E-4 5 2.083853428418176E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.01600399433025159 26-27 0.008773022933640521 28-29 0.0039593215139945345 30-31 0.0013961817970401778 32-33 6.668330970938163E-4 34-35 0.003688420568300172 36-37 7.501872342305434E-4 38-39 3.9593215139945346E-4 40-41 0.0013961817970401778 42-43 7.918643027989069E-4 44-45 3.750936171152717E-4 46-47 0.02606900638951138 48-49 0.015003744684610867 50-51 0.08658410995077521 52-53 0.0012711505913350874 54-55 0.03742600757439044 56-57 8.960569742198156E-4 58-59 0.001041926714209088 60-61 0.0038968059111419893 62-63 0.003500873759742536 64-65 0.008835538536493066 66-67 0.005543050119592348 68-69 0.0017921139484396312 70-71 0.004959571159635259 72-73 0.004063514185415443 74-75 8.543799056514521E-4 76-77 0.0 78-79 0.0 80-81 3.750936171152717E-4 82-83 5.20963357104544E-4 84-85 6.251560285254528E-5 86-87 0.0012503120570509056 88-89 0.0034175196226058084 90-91 0.001041926714209088 92-93 0.0026881709226594473 94-95 7.501872342305434E-4 96-97 3.9593215139945346E-4 98-99 0.0016670827427345408 100-101 0.0027506865255119924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2399401.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.16295917141518 #Duplication Level Percentage of deduplicated Percentage of total 1 85.28097900164731 62.39408784795838 2 9.591464992439262 14.034799232717834 3 2.367283756053869 5.195924543739708 4 0.9479420092882113 2.774169700896907 5 0.48981872105522944 1.7918293544979276 6 0.3154676325851753 1.384832731363931 7 0.19893054316637485 1.018804304534025 8 0.13969808458143507 0.817658020684515 9 0.10307944528840389 0.6787437522342857 >10 0.5308591488531068 6.848618638449154 >50 0.024954008891529444 1.2555452225907096 >100 0.009236778752158108 1.0698856937069539 >500 5.717547958326748E-5 0.03605855713943459 >1k 1.1435095916653496E-4 0.1999953151782329 >5k 1.1435095916653496E-4 0.49904708430795197 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6477 0.2699423731172905 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5453 0.22726505490328627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3516 0.14653657308636614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.167706856836352E-5 2 0.0 0.0 0.0 0.0 4.167706856836352E-5 3 0.0 0.0 0.0 0.0 4.167706856836352E-5 4 0.0 0.0 0.0 0.0 4.167706856836352E-5 5 0.0 0.0 0.0 0.0 4.167706856836352E-5 6 0.0 0.0 0.0 0.0 8.335413713672704E-5 7 0.0 0.0 0.0 0.0 8.335413713672704E-5 8 0.0 0.0 0.0 0.0 8.335413713672704E-5 9 0.0 8.335413713672704E-5 0.0 0.0 8.335413713672704E-5 10-11 0.0 8.335413713672704E-5 0.0 4.167706856836352E-5 1.2503120570509055E-4 12-13 0.0 8.335413713672704E-5 0.0 6.251560285254528E-5 1.2503120570509055E-4 14-15 0.0 8.335413713672704E-5 0.0 8.335413713672704E-5 1.2503120570509055E-4 16-17 0.0 8.335413713672704E-5 0.0 8.335413713672704E-5 1.2503120570509055E-4 18-19 0.0 8.335413713672704E-5 0.0 8.335413713672704E-5 1.2503120570509055E-4 20-21 0.0 8.335413713672704E-5 0.0 8.335413713672704E-5 1.2503120570509055E-4 22-23 0.0 1.2503120570509055E-4 0.0 2.083853428418176E-4 1.2503120570509055E-4 24-25 0.0 2.083853428418176E-4 0.0 4.792862885361805E-4 1.2503120570509055E-4 26-27 0.0 2.083853428418176E-4 0.0 0.001041926714209088 1.2503120570509055E-4 28-29 0.0 2.083853428418176E-4 0.0 0.002417269976965084 1.6670827427345408E-4 30-31 0.0 2.083853428418176E-4 0.0 0.0077936118222839785 2.083853428418176E-4 32-33 0.0 2.083853428418176E-4 0.0 0.020859372818465942 2.083853428418176E-4 34-35 0.0 2.2922387712599935E-4 0.0 0.04315660450254043 2.083853428418176E-4 36-37 0.0 2.500624114101811E-4 0.0 0.07776940994856632 2.083853428418176E-4 38-39 0.0 2.500624114101811E-4 0.0 0.12657325724212 2.083853428418176E-4 40-41 0.0 2.9173947997854465E-4 0.0 0.17814862959546987 2.2922387712599935E-4 42-43 0.0 3.125780142627264E-4 0.0 0.22949477807169372 2.500624114101811E-4 44-45 0.0 3.3341654854690815E-4 0.0 0.2996164459379653 2.9173947997854465E-4 46-47 0.0 3.3341654854690815E-4 0.0 0.3779068192436362 2.9173947997854465E-4 48-49 0.0 3.3341654854690815E-4 0.0 0.4616152114631944 2.9173947997854465E-4 50-51 0.0 3.750936171152717E-4 0.0 0.5488244774424951 3.3341654854690815E-4 52-53 0.0 3.750936171152717E-4 0.0 0.6340124055962301 3.3341654854690815E-4 54-55 0.0 3.750936171152717E-4 0.0 0.7306615276062651 3.3341654854690815E-4 56-57 0.0 3.750936171152717E-4 0.0 0.8458777836635061 3.3341654854690815E-4 58-59 0.0 3.750936171152717E-4 0.0 0.96649122010035 3.3341654854690815E-4 60-61 0.0 3.750936171152717E-4 0.0 1.0881049061828347 3.542550828310899E-4 62-63 0.0 3.750936171152717E-4 0.0 1.2097811078681722 3.750936171152717E-4 64-65 0.0 4.167706856836352E-4 0.0 1.3447731329611017 3.750936171152717E-4 66-67 2.083853428418176E-5 4.3760921996781696E-4 0.0 1.4979155214155533 3.750936171152717E-4 68-69 4.167706856836352E-5 4.584477542519987E-4 0.0 1.663310968029104 4.584477542519987E-4 70-71 4.167706856836352E-5 4.584477542519987E-4 0.0 1.8457731742213994 5.001248228203622E-4 72-73 4.167706856836352E-5 4.584477542519987E-4 0.0 2.0336533993275823 5.001248228203622E-4 74-75 4.167706856836352E-5 4.584477542519987E-4 0.0 2.2302024546959847 5.001248228203622E-4 76-77 4.167706856836352E-5 5.001248228203622E-4 0.0 2.4432139521488905 5.001248228203622E-4 78-79 4.167706856836352E-5 5.418018913887258E-4 0.0 2.6761887654460423 5.001248228203622E-4 80-81 4.167706856836352E-5 5.418018913887258E-4 0.0 2.9353992934069795 5.418018913887258E-4 82-83 4.167706856836352E-5 5.418018913887258E-4 0.0 3.2019033083673802 5.834789599570893E-4 84-85 4.167706856836352E-5 5.418018913887258E-4 0.0 3.4655316055965635 5.834789599570893E-4 86-87 4.167706856836352E-5 5.834789599570893E-4 0.0 3.74257991890476 5.834789599570893E-4 88-89 4.167706856836352E-5 5.834789599570893E-4 0.0 4.047176774536645 6.04317494241271E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3325 0.0 52.506115 1 GTATCAA 5940 0.0 39.641876 1 ATCAACG 7125 0.0 32.393147 3 TCAACGC 7180 0.0 32.14434 4 CAACGCA 7190 0.0 31.967539 5 TATCAAC 7605 0.0 30.473503 2 AACGCAG 7630 0.0 30.24854 6 ACGCAGA 8810 0.0 25.927574 7 CGCAGAG 8910 0.0 25.103596 8 GCAGAGT 9930 0.0 22.28586 9 GTACATG 5765 0.0 21.701607 1 GAGTACT 6500 0.0 21.296976 12-13 ACATGGG 5600 0.0 21.11607 3 TACATGG 5810 0.0 21.088482 2 AGAGTAC 8845 0.0 20.268044 10-11 TACGGGC 235 1.1359407E-7 20.20807 4 CAGAGTA 9955 0.0 19.677748 10-11 TGGTATC 1735 0.0 19.433931 2 GTGGTAT 1765 0.0 19.40543 1 GCGTCGG 250 2.1798041E-7 18.995586 9 >>END_MODULE