FastQCFastQC Report
Sat 18 Jun 2016
SRR1931120_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931120_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019843
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48470.23996914611680215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36690.18164778153549557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21370.10580030230072338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25300.054.6012341
GTATCAA47750.041.7434041
ATCAACG55300.034.9485783
TCAACGC56850.033.917694
CAACGCA57900.033.3585785
AACGCAG60600.032.163156
TATCAAC61200.031.944572
ACGCAGA66700.028.7256377
CGCAGAG67000.028.3841348
GTGGTAT12800.026.8873311
TGGTATC12950.024.7303452
GTACATG51150.024.016661
GCAGAGT77350.023.7256159
TACATGG52700.022.6753792
ACATGGG52250.021.6452053
GTACTAG7200.021.244311
GAGTACT48800.020.61102512-13
CAGAGTA74750.020.5756510-11
AGAGTAC69600.020.08299810-11
CGCAGCG2001.275429E-519.017379
Produced by FastQC (version 0.11.5)