FastQCFastQC Report
Sat 18 Jun 2016
SRR1931120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019843
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56870.281556536819941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49390.24452395557476497No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30700.15199201126028114No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.056.396861
GTATCAA52550.039.8061031
ATCAACG63750.032.404513
TCAACGC64000.032.202934
CAACGCA64500.032.026925
AACGCAG66600.031.5154066
TATCAAC67200.030.6702162
GTGGTAT12600.030.1848561
ACGCAGA77800.026.6122637
TGGTATC13000.026.3018042
CGCAGAG79050.026.1914468
GCAGAGT88600.023.2075379
GAGTACT58700.021.03343212-13
CAGAGTA88500.020.47039210-11
AGAGTAC80150.020.35157810-11
GTACTTT64000.019.43999714-15
GTACATG50900.019.3340241
TACATGG51850.018.5927522
CCGTATA2956.3615516E-817.7079012
ACATGGG52350.017.5987343
Produced by FastQC (version 0.11.5)