##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931120_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2019843 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.15879650052009 31.0 31.0 34.0 28.0 34.0 2 31.162517086724066 31.0 31.0 34.0 28.0 34.0 3 31.31209306861969 31.0 31.0 34.0 28.0 34.0 4 34.989859607900215 35.0 35.0 37.0 32.0 37.0 5 34.87295002631392 35.0 35.0 37.0 32.0 37.0 6 34.98205454582361 36.0 35.0 37.0 32.0 37.0 7 34.85867961024694 35.0 35.0 37.0 32.0 37.0 8 34.88993550488825 35.0 35.0 37.0 32.0 37.0 9 36.3003654244414 38.0 35.0 39.0 32.0 39.0 10-11 36.181196508837566 38.0 35.0 39.0 31.5 39.0 12-13 36.08960547923775 38.0 35.0 39.0 31.0 39.0 14-15 37.01518335831052 39.0 36.0 40.0 31.0 41.0 16-17 37.03549681831707 39.0 36.0 40.0 31.0 41.0 18-19 37.08351441176369 39.0 36.0 40.0 31.0 41.0 20-21 37.06272046886812 39.0 36.0 40.0 31.0 41.0 22-23 36.92859519279469 39.0 36.0 40.0 31.0 41.0 24-25 36.79058545639438 39.0 35.5 40.0 30.5 41.0 26-27 36.50347675537158 38.0 35.0 40.0 30.0 41.0 28-29 36.23236533730592 38.0 35.0 40.0 30.0 41.0 30-31 36.01320350146027 38.0 34.5 40.0 29.0 41.0 32-33 35.83356503451011 38.0 34.0 40.0 29.0 41.0 34-35 35.57393421171844 38.0 34.0 40.0 27.5 41.0 36-37 35.856752975355015 38.0 34.5 40.0 29.0 41.0 38-39 35.83930137144323 38.0 35.0 40.0 29.0 41.0 40-41 35.67051028223481 38.0 34.0 40.0 28.0 41.0 42-43 35.49403889312189 38.0 34.0 40.0 27.0 41.0 44-45 35.272425134032694 38.0 34.0 40.0 27.0 41.0 46-47 35.03633178420303 38.0 33.5 40.0 26.5 41.0 48-49 34.77383489706874 38.0 33.0 40.0 25.5 41.0 50-51 34.41354501315201 38.0 33.0 40.0 24.0 41.0 52-53 34.28162659176976 38.0 33.0 40.0 24.0 41.0 54-55 34.054620581896714 37.5 33.0 40.0 23.5 41.0 56-57 33.82722246233989 37.0 32.5 40.0 23.0 41.0 58-59 33.57072579403449 37.0 32.0 40.0 23.0 41.0 60-61 33.28625541688142 36.5 31.5 39.5 22.0 41.0 62-63 32.99168004641945 36.0 31.0 39.0 21.0 41.0 64-65 32.75297337466328 36.0 31.0 39.0 21.0 40.5 66-67 32.498091930907506 35.5 31.0 39.0 20.0 40.0 68-69 32.166449570585435 35.0 31.0 39.0 19.5 40.0 70-71 31.33937216902502 35.0 30.0 38.0 15.0 40.0 72-73 31.280937181751256 35.0 30.0 37.5 17.5 40.0 74-75 30.970470229616858 34.0 30.0 37.0 15.5 39.0 76-77 30.267726501515217 34.0 29.0 36.0 13.0 39.0 78-79 30.28598707919378 34.0 29.0 36.0 11.5 39.0 80-81 30.010332733781787 34.0 29.0 36.0 8.0 38.0 82-83 29.642857390401137 34.0 29.0 35.0 7.0 37.0 84-85 29.26355786068521 34.0 29.0 35.0 6.0 37.0 86-87 28.916379639407616 34.0 29.0 35.0 2.0 36.0 88-89 28.574789476211766 33.5 28.5 35.0 2.0 36.0 90-91 28.253258297798393 33.0 27.5 35.0 2.0 36.0 92-93 27.87187048696359 33.0 27.0 35.0 2.0 35.0 94-95 27.455301723945873 33.0 26.5 35.0 2.0 35.0 96-97 27.057061118116607 33.0 26.0 35.0 2.0 35.0 98-99 26.540166240643455 33.0 25.0 35.0 2.0 35.0 100-101 25.824663105003705 32.5 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6355820004589887 1101 2 0.47780929103289793 1101 3 0.32980651995351096 1101 4 0.7287305434482434 1101 5 0.6527743353385347 1101 6 0.751524672492188 1101 7 0.5482612813442316 1101 8 0.5619057772947329 1101 9 0.8192217656869829 1101 10-11 0.6172533370923787 1101 12-13 0.7114658690036038 1101 14-15 1.046853092582566 1101 16-17 0.7186492431909173 1101 18-19 0.508038529342727 1101 20-21 0.5641942522108963 1101 22-23 0.47949578665365067 1101 24-25 0.5271773373180508 1101 26-27 0.6564751746420256 1101 28-29 0.522147920730923 1101 30-31 0.7312884892860367 1101 32-33 0.8938704062658402 1101 34-35 0.5594897138964541 1101 36-37 0.9687896736906652 1101 38-39 0.8105534475120209 1101 40-41 0.7972280021604163 1101 42-43 0.8395686507318487 1101 44-45 0.6115198162129545 1101 46-47 0.7034102204714756 1101 48-49 0.5982482534008966 1101 50-51 0.4158642618843231 1101 52-53 0.29587807986893466 1101 54-55 0.33982188828532855 1101 56-57 0.2761840281398946 1101 58-59 0.35412519715980295 1101 60-61 0.6208330128606718 1101 62-63 0.3458855045943139 1101 64-65 0.2606738688376993 1101 66-67 0.0042218810157237385 1101 68-69 -0.008061945996153952 1101 70-71 0.1926739951006411 1101 72-73 -0.19017042428922437 1101 74-75 -0.3259298434010738 1101 76-77 -0.20179668901181813 1101 78-79 -0.13971848268731435 1101 80-81 -0.2170453207482268 1101 82-83 -0.4683138001307974 1101 84-85 -0.2906322107565238 1101 86-87 -0.22994820329206433 1101 88-89 -0.5093308487154431 1101 90-91 -0.359740982290365 1101 92-93 -0.6171469279979078 1101 94-95 -0.7683581363418703 1101 96-97 -0.7480131762194304 1101 98-99 -0.6780060356608786 1101 100-101 -0.9069349186663302 1102 1 0.8848221057651529 1102 2 0.8155015505684453 1102 3 0.575134300220796 1102 4 0.912881789812829 1102 5 0.9060230052825631 1102 6 0.8547636772896183 1102 7 0.870452375570629 1102 8 0.8809357919681773 1102 9 1.1199998923619106 1102 10-11 1.0394664112899505 1102 12-13 1.1149874557790511 1102 14-15 1.6281684833400476 1102 16-17 1.3556969719913 1102 18-19 1.0605806139171534 1102 20-21 1.0760698205874064 1102 22-23 0.8186842905109231 1102 24-25 0.49636700538616196 1102 26-27 0.8489577361914371 1102 28-29 1.0435834723252313 1102 30-31 1.1534171993843572 1102 32-33 1.375129563443089 1102 34-35 1.2713466384248306 1102 36-37 1.4280345374340726 1102 38-39 1.2064795195744722 1102 40-41 1.2760075824514274 1102 42-43 1.4650373554264746 1102 44-45 1.306036520441893 1102 46-47 1.2701050176571584 1102 48-49 1.1851179458076047 1102 50-51 0.9256892815441091 1102 52-53 1.0141063816148161 1102 54-55 0.6855123312126352 1102 56-57 0.9016117520637721 1102 58-59 1.0565162500896577 1102 60-61 1.226681675885338 1102 62-63 1.143730245546422 1102 64-65 1.020634735977083 1102 66-67 0.6095638102888188 1102 68-69 0.7397669655326062 1102 70-71 0.9881081468961597 1102 72-73 0.6009548431497755 1102 74-75 0.55680824595321 1102 76-77 0.6231074420695357 1102 78-79 0.5230953050836398 1102 80-81 0.41400576119903576 1102 82-83 0.430899588733201 1102 84-85 0.35091187054932504 1102 86-87 0.5725351902287734 1102 88-89 0.3514226702765697 1102 90-91 0.2812868923219298 1102 92-93 0.16947670130857517 1102 94-95 0.17576132212435525 1102 96-97 0.3412555044221577 1102 98-99 0.18871900629464733 1102 100-101 0.12327724503050064 1103 1 0.6118386154393747 1103 2 0.45317018977771184 1103 3 0.2556882633695494 1103 4 0.691256448266536 1103 5 0.533933464392419 1103 6 0.6438631456099557 1103 7 0.47465057688555845 1103 8 0.437876663761962 1103 9 0.6374447033151469 1103 10-11 0.5976827842857304 1103 12-13 0.6634136895702838 1103 14-15 1.0944749309430222 1103 16-17 0.8348770132105514 1103 18-19 0.6564765055342434 1103 20-21 0.7189653714405395 1103 22-23 0.5295964739476702 1103 24-25 0.4045671724523885 1103 26-27 0.7279212145245637 1103 28-29 0.5222080909299365 1103 30-31 0.7670233289809261 1103 32-33 0.9866515266056908 1103 34-35 0.7226037764576745 1103 36-37 1.0764961326434985 1103 38-39 0.921916513064275 1103 40-41 0.9723575330546339 1103 42-43 1.0772664470807882 1103 44-45 0.8209094557006011 1103 46-47 0.8723034112593737 1103 48-49 0.9060701819928525 1103 50-51 0.6927836742259004 1103 52-53 0.31024940367045417 1103 54-55 0.4624773933857398 1103 56-57 0.5352929935471735 1103 58-59 0.7700358304346437 1103 60-61 0.9142242297667238 1103 62-63 0.5902326011322145 1103 64-65 0.6030181514919235 1103 66-67 0.5093839670828544 1103 68-69 0.5643726334532708 1103 70-71 0.6948803944615065 1103 72-73 0.25341302432780566 1103 74-75 0.11352563704623364 1103 76-77 0.2841010013777101 1103 78-79 0.29389552245881845 1103 80-81 0.09645396714137888 1103 82-83 0.23304772664259943 1103 84-85 0.17769122368904533 1103 86-87 0.50467951834651 1103 88-89 0.18781502262250882 1103 90-91 0.04406857022022592 1103 92-93 -0.29555064969308376 1103 94-95 -0.3812897087099216 1103 96-97 -0.749850016952692 1103 98-99 -0.8977138608751929 1103 100-101 -0.86368390614712 1104 1 0.34931732415218164 1104 2 0.3587799905437983 1104 3 0.1356340399562903 1104 4 0.6769540750574095 1104 5 0.2309775221006447 1104 6 0.5620011827998113 1104 7 0.37330306239523736 1104 8 0.4527342529134941 1104 9 0.6846711712504074 1104 10-11 0.5492086604163546 1104 12-13 0.5219291445728445 1104 14-15 1.0099585105994038 1104 16-17 0.7156457720244518 1104 18-19 0.5906325950760731 1104 20-21 0.5662313838643627 1104 22-23 0.3090523781234751 1104 24-25 0.3808322833909017 1104 26-27 0.12742600982867458 1104 28-29 0.43740399052128964 1104 30-31 0.6184887462164212 1104 32-33 1.000763451661939 1104 34-35 0.6147876407527377 1104 36-37 0.8785345269384592 1104 38-39 0.5047341398340848 1104 40-41 0.7326538132394802 1104 42-43 0.9474818569103434 1104 44-45 0.7149412200562679 1104 46-47 0.7198616789803651 1104 48-49 0.5003874079196606 1104 50-51 0.38562654969413757 1104 52-53 0.4068421986606907 1104 54-55 0.6613920956539943 1104 56-57 0.6074038529364003 1104 58-59 0.7541855475668058 1104 60-61 0.9312144366633319 1104 62-63 -0.2714723502432719 1104 64-65 -1.0813706824137235 1104 66-67 -0.49311983694290973 1104 68-69 0.18040805852130504 1104 70-71 0.7534367829799535 1104 72-73 0.3061270388761237 1104 74-75 0.06161702793392365 1104 76-77 -0.12426453742071786 1104 78-79 -0.22864072160868432 1104 80-81 -0.20782247273026044 1104 82-83 -0.4302444484728021 1104 84-85 -0.4926870973205624 1104 86-87 -0.11394891131663343 1104 88-89 -0.20012365162398993 1104 90-91 -0.12388701724418993 1104 92-93 -0.12341313546213684 1104 94-95 -0.05513408077280246 1104 96-97 0.03471931809031048 1104 98-99 0.1042256425745336 1104 100-101 -0.14669822181812364 1105 1 -0.09914971002181616 1105 2 -1.4567103388529539 1105 3 -0.4131267211395375 1105 4 0.5124239021476527 1105 5 0.5112798265180558 1105 6 0.2227212740141553 1105 7 -0.23238360796742086 1105 8 -0.1656123825115685 1105 9 0.37709598740956807 1105 10-11 0.8166271480574423 1105 12-13 0.8648376477998667 1105 14-15 1.2527276818250357 1105 16-17 0.7482900465461526 1105 18-19 0.5361273178242243 1105 20-21 0.778913315683738 1105 22-23 0.25406351840737074 1105 24-25 -0.17970339166846117 1105 26-27 0.9752179323383245 1105 28-29 0.5639895196586266 1105 30-31 0.8980320468785621 1105 32-33 1.1375173926027458 1105 34-35 0.5326048773347054 1105 36-37 0.8065118070355339 1105 38-39 0.6920856811681944 1105 40-41 1.1104259656678508 1105 42-43 1.4860780765360744 1105 44-45 1.338699209156573 1105 46-47 1.4927924627751779 1105 48-49 1.4464215714569377 1105 50-51 0.7821761997950816 1105 52-53 1.0455083980979154 1105 54-55 0.13316502214904347 1105 56-57 0.2958873098763206 1105 58-59 -0.41727116223140825 1105 60-61 0.2710577404635828 1105 62-63 0.9073453152134832 1105 64-65 1.4042284347318486 1105 66-67 1.2207845972038882 1105 68-69 1.28621209412837 1105 70-71 0.4462735281341921 1105 72-73 -0.13072721692153166 1105 74-75 -0.12398512271618856 1105 76-77 0.1816196245475048 1105 78-79 -0.39720378189174 1105 80-81 -0.2843776668222482 1105 82-83 -0.22037018200557767 1105 84-85 -0.16352016042791817 1105 86-87 -0.26189303207103265 1105 88-89 -0.6184630694003275 1105 90-91 -0.8360049264273428 1105 92-93 -0.3417900013289348 1105 94-95 -0.5605555809977005 1105 96-97 -0.6208529159288751 1105 98-99 -1.2810037008286415 1105 100-101 -0.5633690028932428 1106 1 0.2807731671513878 1106 2 0.3498030017168894 1106 3 -0.21597810677166152 1106 4 0.454238507507867 1106 5 0.05444486097145074 1106 6 0.4314504265626269 1106 7 0.34830522001748676 1106 8 0.39306609446253304 1106 9 0.7625610332574695 1106 10-11 0.1012435429551033 1106 12-13 0.18880371615492209 1106 14-15 0.00480586014309381 1106 16-17 -0.3691995034907265 1106 18-19 0.25726143118158973 1106 20-21 0.35073895572523384 1106 22-23 0.3772929618156482 1106 24-25 0.4820848264104285 1106 26-27 0.7611050547453289 1106 28-29 0.536174326581687 1106 30-31 0.08054249684168013 1106 32-33 0.29504874249209223 1106 34-35 -1.264554771714451 1106 36-37 -0.794120288294522 1106 38-39 -0.867734645434382 1106 40-41 0.48962067381852137 1106 42-43 0.7649779667889618 1106 44-45 0.3581363204689154 1106 46-47 -0.051422605561889156 1106 48-49 -0.05001920408925287 1106 50-51 0.3100912447434183 1106 52-53 -0.2960617650834294 1106 54-55 0.4908532564201238 1106 56-57 0.06250685925351718 1106 58-59 0.5139031317588874 1106 60-61 0.6885267803863329 1106 62-63 0.3291437883494268 1106 64-65 0.35391646510121433 1106 66-67 0.17568243644537773 1106 68-69 0.1749805277433687 1106 70-71 0.4619439749788157 1106 72-73 -0.23338429620931578 1106 74-75 -0.10390596087219706 1106 76-77 -0.009341292290582004 1106 78-79 -0.05108283218949694 1106 80-81 0.025665692278664665 1106 82-83 -0.2609649753152006 1106 84-85 -0.14937501838638312 1106 86-87 0.06727738778659287 1106 88-89 -0.018686221840852113 1106 90-91 0.06702949098346522 1106 92-93 -0.4702326119905962 1106 94-95 -0.20719618022887332 1106 96-97 0.1309291251273379 1106 98-99 0.179838893041115 1106 100-101 0.12022608427474424 1107 1 0.4620747797741025 1107 2 0.4553627008892569 1107 3 0.20998820904306115 1107 4 -1.1121947874334452 1107 5 0.10711226475650903 1107 6 0.4612604791699013 1107 7 0.6481319666834935 1107 8 0.5498333747387036 1107 9 0.912778049708173 1107 10-11 0.869946159872697 1107 12-13 0.9284896349104983 1107 14-15 1.1986890137262236 1107 16-17 0.9290741496610835 1107 18-19 0.8128190471475278 1107 20-21 0.04296272492111797 1107 22-23 0.6066091682462584 1107 24-25 0.6450549327139186 1107 26-27 0.9154542373866619 1107 28-29 0.8646876936650756 1107 30-31 0.9207065903801706 1107 32-33 1.0480866729464609 1107 34-35 0.9611333413518963 1107 36-37 1.2001208295919241 1107 38-39 1.1689600098470407 1107 40-41 0.6148190913391716 1107 42-43 0.29897818394745457 1107 44-45 -0.09487758998413653 1107 46-47 0.6458107227107277 1107 48-49 1.055465610125033 1107 50-51 -1.0902727797967557 1107 52-53 0.8358950790861073 1107 54-55 1.0092337654358232 1107 56-57 0.9183148071910381 1107 58-59 1.0888620278867407 1107 60-61 1.2021318543196529 1107 62-63 1.0587846277806108 1107 64-65 1.1611994336577425 1107 66-67 0.942911342638908 1107 68-69 1.109316283172408 1107 70-71 1.2891843766841333 1107 72-73 0.89077654247696 1107 74-75 0.6904294852322543 1107 76-77 1.0603199467816609 1107 78-79 0.8705083555948292 1107 80-81 0.9515514416632485 1107 82-83 0.8820680564476966 1107 84-85 0.81733913065694 1107 86-87 1.1348738852221985 1107 88-89 1.1420310229734625 1107 90-91 1.1658103259894155 1107 92-93 1.049552640991152 1107 94-95 1.066200595156971 1107 96-97 1.208040746799373 1107 98-99 1.2340446342948823 1107 100-101 1.1137416308339319 1108 1 0.4379023528768222 1108 2 0.4572479409864094 1108 3 0.09965801228075577 1108 4 0.5160499202448747 1108 5 0.3947269265457862 1108 6 0.24934165341905867 1108 7 0.4134724153679272 1108 8 0.5128059078040579 1108 9 0.6061158409473606 1108 10-11 0.40573153062576495 1108 12-13 0.5107578638983838 1108 14-15 0.8359073291243249 1108 16-17 0.47612947220066815 1108 18-19 0.3702896792962349 1108 20-21 0.30849426532579827 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-0.6415570422496373 2311 66-67 1.511885688803325 2311 68-69 1.9478494932740738 2311 70-71 2.2877543064920474 2311 72-73 1.7337435946589075 2311 74-75 1.5534424994954854 2311 76-77 1.8169020165740797 2311 78-79 2.031856493381156 2311 80-81 1.4915122240178107 2311 82-83 1.592225926205149 2311 84-85 1.605089236819353 2311 86-87 1.8522882683561157 2311 88-89 1.887797016029193 2311 90-91 1.9410145325190271 2311 92-93 1.8876736911247924 2311 94-95 2.355305833335475 2311 96-97 2.8499112036724554 2311 98-99 3.0745824406046793 2311 100-101 3.262606317467462 2312 1 -0.14943805376341146 2312 2 -0.05083350495687711 2312 3 -0.29619541567445395 2312 4 0.187308415250115 2312 5 -1.333088058263968 2312 6 0.008019030012597739 2312 7 0.2697026262334319 2312 8 -2.8156688560526675 2312 9 -5.17325092148576 2312 10-11 -0.6686649307067185 2312 12-13 -5.893549121256218 2312 14-15 -2.2940622903863925 2312 16-17 -3.373212627729991 2312 18-19 -2.8325209028110265 2312 20-21 -3.9668419058778 2312 22-23 -2.0990144389834953 2312 24-25 -3.4114480954064703 2312 26-27 -3.723006587337771 2312 28-29 -6.172037013941633 2312 30-31 -6.2777898074854335 2312 32-33 -1.9798017576049247 2312 34-35 0.1554787530929218 2312 36-37 0.8065685793784354 2312 38-39 0.7700442672380561 2312 40-41 -3.3794781189528216 2312 42-43 -5.1148595635272756 2312 44-45 -3.940086460308869 2312 46-47 0.11928931041613566 2312 48-49 -1.6447461459960806 2312 50-51 -6.5473349604523285 2312 52-53 -1.1067550255731575 2312 54-55 0.7156594196152 2312 56-57 1.020440152975496 2312 58-59 1.130528760080118 2312 60-61 1.6694743897001416 2312 62-63 1.1931172696760868 2312 64-65 1.3232211738479975 2312 66-67 0.7144753970963222 2312 68-69 0.9117886322106621 2312 70-71 1.4508003754763479 2312 72-73 1.163910485890188 2312 74-75 0.786883494130258 2312 76-77 0.8803897563191008 2312 78-79 0.620871635683784 2312 80-81 0.7066224228182918 2312 82-83 -0.18608745573791197 2312 84-85 0.13076256583974555 2312 86-87 1.109023990667783 2312 88-89 1.0483696314691855 2312 90-91 1.2442108084756995 2312 92-93 1.393214032495429 2312 94-95 1.4896676239504565 2312 96-97 1.443506004799005 2312 98-99 1.3620721649495877 2312 100-101 1.5545151815272327 2313 1 0.9212584532434924 2313 2 1.1329495250926271 2313 3 0.586465115595157 2313 4 -1.4143788189407829 2313 5 -1.9571555476316007 2313 6 -1.8795916511559128 2313 7 0.12217421516182725 2313 8 -0.8189457158448974 2313 9 -0.2682101350394106 2313 10-11 0.29574279431594164 2313 12-13 -2.4319650732885663 2313 14-15 -2.190945787562711 2313 16-17 -5.088648728964046 2313 18-19 -0.858770641359456 2313 20-21 -1.944147908806059 2313 22-23 0.030526103441381736 2313 24-25 1.2332217783995674 2313 26-27 -1.6280473737841277 2313 28-29 -1.8720649021100826 2313 30-31 -0.7817638714887849 2313 32-33 -8.149013219642153 2313 34-35 0.192604877334702 2313 36-37 0.28882473220559746 2313 38-39 -0.8545809854984725 2313 40-41 0.4443035166882652 2313 42-43 -2.151881107137399 2313 44-45 -4.2015728996869655 2313 46-47 -0.31489461205475777 2313 48-49 2.2422719115929937 2313 50-51 3.6818360637406613 2313 52-53 3.7679573776897612 2313 54-55 1.1871786275912157 2313 56-57 -4.819690921416196 2313 58-59 0.8583750962719918 2313 60-61 -2.513227973822133 2313 62-63 2.2313589206556586 2313 64-65 1.184024353099204 2313 66-67 -1.1713922735444058 2313 68-69 -2.850046409272988 2313 70-71 -2.03671966914472 2313 72-73 3.1634224429424087 2313 74-75 3.632069299052514 2313 76-77 1.4016196245475037 2313 78-79 3.539054721509622 2313 80-81 4.564601925014486 2313 82-83 4.905071994865171 2313 84-85 4.817160111680927 2313 86-87 4.905998124391548 2313 88-89 2.8440539374023928 2313 90-91 5.091069903504632 2313 92-93 4.621339250371747 2313 94-95 4.811009044852636 2313 96-97 5.864861369785409 2313 98-99 5.332057523661156 2313 100-101 6.211665010712199 2314 1 -28.65017011818508 2314 2 -14.62895523681214 2314 3 -4.7627412336111945 2314 4 -3.2715216760836405 2314 5 -0.6873142777903354 2314 6 -0.16530593687019746 2314 7 -0.14766705467943808 2314 8 -4.152279049178233 2314 9 -2.9031307699600433 2314 10-11 0.2401872387603845 2314 12-13 0.6077174663939715 2314 14-15 -1.0560251526420785 2314 16-17 -0.3188074591227732 2314 18-19 -3.3428976254864367 2314 20-21 -3.5870050516631977 2314 22-23 -5.652013579098295 2314 24-25 -0.07630203112424283 2314 26-27 -3.056618802355551 2314 28-29 -2.491112521157703 2314 30-31 -3.765890855615769 2314 32-33 -2.458537029165967 2314 34-35 -0.7677125829827531 2314 36-37 -5.814349870969004 2314 38-39 -6.021247652165137 2314 40-41 -4.508077435692691 2314 42-43 -3.88203983729613 2314 44-45 -3.9317316298456966 2314 46-47 -4.433942231102378 2314 48-49 -5.900585231264152 2314 50-51 -2.7308623489577535 2314 52-53 -3.7717251619927836 2314 54-55 -5.685837245424658 2314 56-57 -2.7403258420511136 2314 58-59 -2.6416249037280117 2314 60-61 0.23280377220961412 2314 62-63 0.9218351111318484 2314 64-65 0.7237068927817347 2314 66-67 0.9635283613762269 2314 68-69 1.6340805748539928 2314 70-71 1.8918517594267072 2314 72-73 1.4808827604027321 2314 74-75 1.282862949846173 2314 76-77 -0.4713962484683698 2314 78-79 -0.8022151197602234 2314 80-81 0.2630146234271855 2314 82-83 -0.7854041956110223 2314 84-85 0.6901759846968005 2314 86-87 2.152029870423295 2314 88-89 1.804371397719855 2314 90-91 1.694244506679233 2314 92-93 2.2721329011653957 2314 94-95 2.9459296797732755 2314 96-97 2.0077185126425547 2314 98-99 2.7923749839786147 2314 100-101 2.7275380265852185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 14.0 4 59.0 5 90.0 6 242.0 7 641.0 8 1457.0 9 2629.0 10 3862.0 11 5567.0 12 7216.0 13 9089.0 14 11089.0 15 13553.0 16 15468.0 17 17316.0 18 16030.0 19 13895.0 20 13716.0 21 14344.0 22 15788.0 23 18154.0 24 21574.0 25 26728.0 26 32975.0 27 41630.0 28 52033.0 29 63787.0 30 77501.0 31 93858.0 32 111329.0 33 133664.0 34 161539.0 35 196638.0 36 242597.0 37 289827.0 38 244556.0 39 49357.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.536806294866686 21.931421003903562 15.627087831153563 22.90468487007619 2 15.223064366883962 22.288861064944157 39.73189995460043 22.75617461357145 3 18.36344372434758 26.61341030503171 31.319147040038814 23.703998930581893 4 12.995207038983839 18.681606486067935 38.56746856305732 29.755717911890912 5 12.283157221936996 38.4848495350372 36.12837458827164 13.103618654754165 6 29.594131821136592 39.32008576904244 17.901242819367642 13.184539590453317 7 25.681600005544986 33.35744411818146 23.048375542059457 17.912580334214095 8 24.42506670072872 36.976537285323666 20.58664955642592 18.0117464575217 9 24.91436215587053 16.47340907189321 21.307349135551625 37.30487963668463 10-11 23.675429228905415 27.42883976625906 29.189694446548568 19.706036558286957 12-13 24.432567283694823 24.97800571628587 29.19811094228611 21.3913160577332 14-15 21.634701310943473 26.656849071932818 26.909517224853612 24.798932392270093 16-17 21.095476232558667 29.64007598610387 27.61412644448108 21.65032133685638 18-19 20.972644903589043 28.57026511466485 29.737583564663193 20.71950641708291 20-21 21.766543241232117 27.647792427431238 29.80320747701678 20.782456854319868 22-23 21.51998942492065 27.55701804546195 29.590171117260105 21.332821412357298 24-25 21.38675745806852 27.73012335024036 29.663649446950334 21.219469744740785 26-27 21.433611098732975 27.775277394055525 29.566393852521923 21.22471765468958 28-29 21.354279871964035 27.840519471517446 29.610470628717984 21.19473002780054 30-31 21.678844476218863 27.634872665232198 29.567619272064377 21.118663586484562 32-33 21.396284219667315 27.765422429149556 29.50297997146277 21.33531337972035 34-35 21.601594816894604 27.745169925829032 29.544908809962212 21.108326447314155 36-37 21.56404879554593 27.628860160742402 29.518017858561834 21.28907318514983 38-39 21.459382051190016 27.824059232781018 29.522842916041096 21.19371579998787 40-41 21.663546700716463 27.65895611765003 29.463081998250313 21.2144151833832 42-43 21.57274499489433 27.670612990067145 29.306928242101684 21.449713772936843 44-45 21.6671011092768 27.84868722830318 29.13731084727696 21.346900815143062 46-47 21.712815898376693 27.76280697201828 28.961910324887256 21.562466804717772 48-49 21.710769337420093 27.890623065762828 28.925616356280916 21.472991240536167 50-51 21.71802986899918 28.02754500691004 28.693599209941734 21.56082591414905 52-53 21.75470099814985 28.00787885359066 28.63377094290282 21.60364920535667 54-55 21.739398527409193 28.01302762203552 28.616862657988516 21.630711192566768 56-57 21.79143693353708 28.05675539792918 28.50233844458345 21.649469223950287 58-59 21.79555159481638 28.087161016424687 28.537472305769064 21.57981508298986 60-61 21.841368735676117 28.118701390391383 28.51416309905332 21.52576677487918 62-63 21.746426727174697 28.076772451748628 28.484562976195015 21.692237844881664 64-65 21.64389130211972 28.01377266292774 28.511952300717002 21.830383734235532 66-67 21.700399606483415 28.01295416185375 28.460066830483377 21.826579401179462 68-69 21.73378981530304 27.992939877561835 28.433058800521838 21.840211506613294 70-71 21.818093597852986 27.824768230905367 28.318025643444084 22.03911252779756 72-73 21.775382212955954 27.879897076421795 28.35983247439907 21.984888236223178 74-75 21.651388092614102 28.027791557869953 28.294597246688074 22.026223102827874 76-77 21.612125298847484 27.988957557592347 28.18045759002061 22.218459553539557 78-79 21.638761032416877 28.042055743936533 28.111145272182046 22.208037951464544 80-81 21.618013497101998 28.006709439010297 28.09391927384383 22.28135779004387 82-83 21.658706461257555 27.95341608910413 28.234700884575453 22.15317656506286 84-85 21.665773445206742 27.998990020261484 28.04812751489287 22.287109019638905 86-87 21.517511769653925 28.012543735484424 28.11455812988628 22.35538636497537 88-89 21.642291694619615 27.819947191823992 28.08658738673795 22.451173726818443 90-91 21.62023000160411 27.820336744211836 28.110809322847867 22.448623931336183 92-93 21.64388322778958 27.829784305556277 28.042530406180678 22.48380206047346 94-95 21.441028285322243 27.803177348967996 28.04800011486124 22.707794250848526 96-97 21.427057867131655 27.71769858315089 28.077950960326444 22.77729258939101 98-99 21.34807401200362 27.7705919994851 27.989053412268248 22.89228057624303 100-101 21.377793455994752 27.73294220307829 27.941157176188092 22.948107164738865 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 274.0 1 198.0 2 148.0 3 176.0 4 157.5 5 162.5 6 273.0 7 534.0 8 853.5 9 1095.5 10 1158.0 11 1114.5 12 1201.0 13 1387.0 14 1606.0 15 1785.0 16 1901.0 17 2070.0 18 2326.0 19 2622.5 20 3016.0 21 3668.0 22 4482.0 23 5779.5 24 7577.0 25 9892.0 26 13283.5 27 17190.0 28 21486.5 29 26557.5 30 31644.0 31 36786.5 32 42444.5 33 47820.0 34 52788.5 35 57359.5 36 62275.0 37 67156.0 38 69607.5 39 70648.5 40 71709.0 41 72900.5 42 73864.0 43 73725.0 44 73671.5 45 74488.0 46 73807.5 47 72324.5 48 71364.0 49 69541.5 50 67120.5 51 64472.0 52 61186.0 53 57627.0 54 54210.5 55 49788.0 56 44999.0 57 40212.5 58 35272.0 59 30664.5 60 25735.5 61 20682.5 62 16227.0 63 12580.5 64 9575.0 65 7132.0 66 5285.0 67 4016.5 68 3129.0 69 2400.5 70 1843.0 71 1422.0 72 1067.5 73 847.5 74 665.0 75 473.0 76 363.5 77 289.0 78 214.0 79 147.0 80 103.0 81 85.0 82 72.0 83 45.0 84 34.0 85 28.5 86 21.5 87 15.5 88 9.0 89 6.5 90 5.0 91 2.0 92 1.5 93 1.5 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10886984780500265 2 0.0 3 0.0026239663181742345 4 2.970527907367058E-4 5 1.9803519382447052E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.013293112385467584 26-27 0.0075748461637859974 28-29 0.004851862248699528 30-31 0.002425931124349764 32-33 0.0012377199614029407 34-35 0.0024754399228058815 36-37 0.0013614919575432349 38-39 7.673863760698233E-4 40-41 0.002772492713542587 42-43 0.0015100183529115877 44-45 6.68368779157588E-4 46-47 0.02685852316244381 48-49 0.015496253916764818 50-51 0.0873335204765915 52-53 2.970527907367058E-4 54-55 0.03705733564440404 56-57 8.664039729820584E-4 58-59 8.911583722101173E-4 60-61 0.0025744575197181167 62-63 0.002772492713542587 64-65 0.009406671706662349 66-67 0.005247932636348469 68-69 0.0023764223258936463 70-71 0.005272687035576527 72-73 0.004554809457962822 74-75 0.00103968476757847 76-77 0.0 78-79 0.0 80-81 9.901759691223526E-5 82-83 1.9803519382447052E-4 84-85 2.4754399228058815E-5 86-87 0.0014852639536835288 88-89 0.00396070387648941 90-91 0.0015347727521396466 92-93 0.003193317500419587 94-95 7.178775776137056E-4 96-97 4.4557918610505865E-4 98-99 0.001757562345192176 100-101 0.0027477383143145284 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2019843.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.55686936087375 #Duplication Level Percentage of deduplicated Percentage of total 1 86.41481285787698 65.29232725946963 2 9.098701933286485 13.749388666537124 3 2.157007486766339 4.889301986640925 4 0.8294174936245773 2.506727568456621 5 0.4174675821470661 1.5771271783342848 6 0.24570619101546326 1.1138874345427947 7 0.16515915829060496 0.8735236262700575 8 0.11727024771481351 0.7088458229204373 9 0.0878543181443396 0.5974197514938456 >10 0.4367277068622359 5.879498546353389 >50 0.02231182758117424 1.143085135210221 >100 0.007234722004290379 0.8885727893965729 >500 6.569493711152432E-5 0.03921325986994945 >1k 1.9708481133457297E-4 0.45879514047840864 >5k 6.569493711152432E-5 0.282285834025826 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5687 0.281556536819941 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4939 0.24452395557476497 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3070 0.15199201126028114 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 4.950879845611763E-5 0.0 0.0 0.0 9 0.0 4.950879845611763E-5 0.0 4.950879845611763E-5 0.0 10-11 0.0 4.950879845611763E-5 0.0 4.950879845611763E-5 0.0 12-13 0.0 4.950879845611763E-5 0.0 4.950879845611763E-5 0.0 14-15 0.0 4.950879845611763E-5 0.0 7.426319768417645E-5 0.0 16-17 4.950879845611763E-5 4.950879845611763E-5 0.0 9.901759691223526E-5 0.0 18-19 4.950879845611763E-5 4.950879845611763E-5 0.0 9.901759691223526E-5 0.0 20-21 4.950879845611763E-5 4.950879845611763E-5 0.0 9.901759691223526E-5 0.0 22-23 4.950879845611763E-5 4.950879845611763E-5 0.0 9.901759691223526E-5 0.0 24-25 7.426319768417645E-5 9.901759691223526E-5 0.0 2.475439922805881E-4 0.0 26-27 9.901759691223526E-5 1.485263953683529E-4 0.0 5.445967830172939E-4 0.0 28-29 9.901759691223526E-5 1.485263953683529E-4 0.0 0.001336737558315176 0.0 30-31 9.901759691223526E-5 2.475439922805881E-4 0.0 0.0055944942255412924 0.0 32-33 9.901759691223526E-5 2.475439922805881E-4 0.0 0.017006272269676406 2.4754399228058815E-5 34-35 1.7328079459641172E-4 2.475439922805881E-4 0.0 0.035794861283773044 9.901759691223526E-5 36-37 1.9803519382447052E-4 2.475439922805881E-4 0.0 0.0667131059196185 9.901759691223526E-5 38-39 1.9803519382447052E-4 2.9705279073670573E-4 0.0 0.10953821658416026 9.901759691223526E-5 40-41 1.9803519382447052E-4 3.465615891928234E-4 0.0 0.15270988883789482 9.901759691223526E-5 42-43 1.9803519382447052E-4 3.465615891928234E-4 0.0 0.19952045777815403 9.901759691223526E-5 44-45 1.9803519382447052E-4 3.465615891928234E-4 0.0 0.26115891185602047 9.901759691223526E-5 46-47 1.9803519382447052E-4 3.465615891928234E-4 0.0 0.33455570556721487 1.485263953683529E-4 48-49 1.9803519382447052E-4 3.465615891928234E-4 0.0 0.4118389399572145 1.485263953683529E-4 50-51 2.475439922805881E-4 4.4557918610505865E-4 0.0 0.489914315122512 1.485263953683529E-4 52-53 2.475439922805881E-4 4.4557918610505865E-4 0.0 0.5678411638924412 1.485263953683529E-4 54-55 2.475439922805881E-4 4.4557918610505865E-4 0.0 0.6534666308222966 1.485263953683529E-4 56-57 2.475439922805881E-4 4.4557918610505865E-4 0.0 0.7570142827932667 1.485263953683529E-4 58-59 2.475439922805881E-4 4.4557918610505865E-4 0.0 0.8688299041064083 1.7328079459641172E-4 60-61 2.475439922805881E-4 4.4557918610505865E-4 0.0 0.982328824567058 1.9803519382447052E-4 62-63 2.475439922805881E-4 4.4557918610505865E-4 0.0 1.0989963081288991 2.475439922805881E-4 64-65 2.475439922805881E-4 4.4557918610505865E-4 0.0 1.221827637098527 2.475439922805881E-4 66-67 2.970527907367058E-4 4.4557918610505865E-4 0.0 1.3627791863030938 2.475439922805881E-4 68-69 3.465615891928234E-4 4.4557918610505865E-4 0.0 1.5251927996383876 2.475439922805881E-4 70-71 3.465615891928234E-4 4.7033358533311745E-4 0.0 1.6950822415405553 2.475439922805881E-4 72-73 3.465615891928234E-4 4.950879845611763E-4 0.0 1.8743288463509291 2.475439922805881E-4 74-75 3.465615891928234E-4 4.950879845611763E-4 0.0 2.058452067809231 2.475439922805881E-4 76-77 3.465615891928234E-4 5.445967830172939E-4 0.0 2.2643839149874516 2.475439922805881E-4 78-79 3.9607038764894104E-4 5.941055814734116E-4 0.0 2.487866631218367 2.475439922805881E-4 80-81 3.9607038764894104E-4 5.941055814734116E-4 0.0 2.7347917635182535 2.7229839150864693E-4 82-83 3.9607038764894104E-4 5.941055814734116E-4 0.0 2.9907027427379256 2.970527907367058E-4 84-85 3.9607038764894104E-4 5.941055814734116E-4 0.0 3.24990605705493 2.970527907367058E-4 86-87 3.9607038764894104E-4 5.941055814734116E-4 0.0 3.512921548853054 2.970527907367058E-4 88-89 3.9607038764894104E-4 6.931231783856468E-4 0.0 3.8085880932329887 3.218071899647646E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3060 0.0 56.39686 1 GTATCAA 5255 0.0 39.806103 1 ATCAACG 6375 0.0 32.40451 3 TCAACGC 6400 0.0 32.20293 4 CAACGCA 6450 0.0 32.02692 5 AACGCAG 6660 0.0 31.515406 6 TATCAAC 6720 0.0 30.670216 2 GTGGTAT 1260 0.0 30.184856 1 ACGCAGA 7780 0.0 26.612263 7 TGGTATC 1300 0.0 26.301804 2 CGCAGAG 7905 0.0 26.191446 8 GCAGAGT 8860 0.0 23.207537 9 GAGTACT 5870 0.0 21.033432 12-13 CAGAGTA 8850 0.0 20.470392 10-11 AGAGTAC 8015 0.0 20.351578 10-11 GTACTTT 6400 0.0 19.439997 14-15 GTACATG 5090 0.0 19.334024 1 TACATGG 5185 0.0 18.592752 2 CCGTATA 295 6.3615516E-8 17.707901 2 ACATGGG 5235 0.0 17.598734 3 >>END_MODULE