FastQCFastQC Report
Sat 18 Jun 2016
SRR1931119_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931119_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046384
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79010.3860956692390089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58710.2868963009874979No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34510.16863892602756864No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA24600.12021204231463889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35150.057.3651541
GTATCAA73100.045.4708941
ATCAACG91350.035.575983
CAACGCA91450.035.487145
TCAACGC98250.033.907414
TATCAAC98950.033.301522
AACGCAG99550.033.0566146
CGCAGAG106100.029.9056348
ACGCAGA108500.029.4202257
GCAGAGT110850.027.3796259
GTACATG88650.024.3122521
GTACTAG10700.024.171381
GAGTACT61700.023.55681212-13
GTGGTAT18350.022.9687841
GCATCGT9200.022.7513648
CAGAGTA111450.022.70552410-11
TACATGG92550.022.6667422
ACATGGG92300.022.5942573
TGGTATC18550.022.4629482
CTGTGCG10050.022.2471499
Produced by FastQC (version 0.11.5)