##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931119_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2046384 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.019036505367517 31.0 28.0 33.0 25.0 34.0 2 28.747104648980837 31.0 28.0 33.0 22.0 34.0 3 28.787738762617376 31.0 28.0 34.0 22.0 34.0 4 32.03326208570825 35.0 32.0 37.0 19.0 37.0 5 32.15229350894065 35.0 32.0 37.0 22.0 37.0 6 32.41599719309768 35.0 32.0 37.0 25.0 37.0 7 32.11942089070282 35.0 32.0 37.0 22.0 37.0 8 32.31808204129821 35.0 32.0 37.0 23.0 37.0 9 33.3242993494867 37.0 32.0 39.0 21.0 39.0 10-11 33.266909827285595 37.0 32.0 39.0 21.0 39.0 12-13 33.2801016329291 37.0 32.0 39.0 22.0 39.0 14-15 34.08211191057006 38.0 32.0 40.0 21.5 41.0 16-17 34.09579433771961 38.0 32.0 40.0 22.0 41.0 18-19 34.11589858012963 38.0 32.0 40.0 21.0 41.0 20-21 34.33433510035262 38.0 32.0 40.0 24.0 41.0 22-23 34.22809306562209 38.0 32.0 40.0 23.5 41.0 24-25 34.11858013940687 38.0 32.0 40.0 23.0 41.0 26-27 33.776007337821255 38.0 32.0 40.0 19.5 41.0 28-29 33.7047611787426 38.0 32.0 40.0 20.5 41.0 30-31 33.39018581067874 37.5 31.5 40.0 18.5 41.0 32-33 33.1414727147984 37.0 31.0 40.0 17.5 41.0 34-35 33.10407406430074 37.0 31.0 40.0 18.0 41.0 36-37 32.731554537173864 37.0 30.5 40.0 15.5 41.0 38-39 32.550381306734224 37.0 30.0 40.0 15.5 41.0 40-41 32.4415429850898 36.5 30.0 40.0 14.5 41.0 42-43 32.63824653632945 37.0 30.5 40.0 15.0 41.0 44-45 32.57053514882837 37.0 30.5 40.0 12.0 41.0 46-47 32.25860517869569 37.0 30.0 40.0 9.0 41.0 48-49 31.973763966098247 37.0 30.0 40.0 8.0 41.0 50-51 31.460163390644183 35.5 30.0 39.5 6.5 40.5 52-53 31.378676973627627 36.0 30.0 39.5 2.0 40.5 54-55 31.33974806292465 36.0 29.5 40.0 2.0 41.0 56-57 31.07413051509394 36.0 29.0 40.0 2.0 41.0 58-59 30.871111433631224 35.0 29.0 39.0 2.0 41.0 60-61 30.697394526149537 35.0 29.0 39.0 2.0 41.0 62-63 30.522111197116473 35.0 29.0 39.0 2.0 40.5 64-65 30.173032773907536 35.0 28.0 39.0 2.0 40.0 66-67 29.918581507674023 34.0 28.0 38.0 2.0 40.0 68-69 29.72302192550372 34.0 28.0 38.0 2.0 40.0 70-71 29.456824818802335 34.0 28.0 37.5 2.0 40.0 72-73 29.11983576884886 34.0 28.0 37.0 2.0 40.0 74-75 28.707685605438666 34.0 27.5 36.5 2.0 39.0 76-77 28.41872835205905 34.0 27.0 36.0 2.0 39.0 78-79 28.032980613609176 33.5 26.5 36.0 2.0 38.5 80-81 27.654368388337673 33.0 26.0 35.0 2.0 37.0 82-83 27.33013427587393 33.0 26.0 35.0 2.0 37.0 84-85 26.999872702288524 33.0 26.0 35.0 2.0 36.5 86-87 26.695682237546812 33.0 25.0 35.0 2.0 36.0 88-89 26.37436717644391 33.0 25.0 35.0 2.0 36.0 90-91 25.992085307547363 32.0 24.0 35.0 2.0 35.5 92-93 25.63309134551482 32.0 22.5 35.0 2.0 35.0 94-95 25.349126068225708 32.0 20.5 35.0 2.0 35.0 96-97 24.95894807621639 32.0 18.5 35.0 2.0 35.0 98-99 24.60702487900609 32.0 9.0 35.0 2.0 35.0 100-101 24.008074242175468 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.045272403713312 1101 2 5.726013632255604 1101 3 5.3230260465642445 1101 4 6.610827232996524 1101 5 6.030696921381775 1101 6 5.634902851945277 1101 7 6.222583425437264 1101 8 5.6007441093012 1101 9 6.2880609784548795 1101 10-11 6.35016111519289 1101 12-13 6.195729015298955 1101 14-15 6.409041295898984 1101 16-17 6.400603951711183 1101 18-19 6.538213478327407 1101 20-21 5.896917274506361 1101 22-23 5.912011972689104 1101 24-25 5.941358070413759 1101 26-27 6.029671158484856 1101 28-29 5.969188950583476 1101 30-31 6.079173057378007 1101 32-33 5.920894418828144 1101 34-35 5.7626188253550055 1101 36-37 5.846776459695366 1101 38-39 5.710492664867434 1101 40-41 5.697587130172558 1101 42-43 5.681471589303666 1101 44-45 5.73114621229108 1101 46-47 5.527984661853932 1101 48-49 5.29186737237686 1101 50-51 5.2331634850181565 1101 52-53 5.063914585890981 1101 54-55 5.295930681609427 1101 56-57 5.220145671125646 1101 58-59 5.057903921213207 1101 60-61 4.989117430686196 1101 62-63 4.917001684184772 1101 64-65 5.02392432160428 1101 66-67 4.946730373707197 1101 68-69 4.98772245049458 1101 70-71 4.8975438724840075 1101 72-73 4.62917195832501 1101 74-75 4.826864532564169 1101 76-77 4.591723564912531 1101 78-79 4.504658977738131 1101 80-81 4.542538995773604 1101 82-83 4.495026720056909 1101 84-85 4.331441681122879 1101 86-87 4.134114419759925 1101 88-89 4.240557093937728 1101 90-91 4.14523870742984 1101 92-93 4.146932868842391 1101 94-95 4.068942680165971 1101 96-97 3.9590934320624207 1101 98-99 3.7372256150849132 1101 100-101 3.760941044127076 1102 1 5.234409619977157 1102 2 5.86601220944933 1102 3 5.566088761098978 1102 4 6.89656002170965 1102 5 6.395588777412598 1102 6 5.887762899274264 1102 7 6.401283758912832 1102 8 5.971251986986051 1102 9 6.843006606012956 1102 10-11 6.764201233482545 1102 12-13 6.74860825910736 1102 14-15 7.013534262916256 1102 16-17 6.9305318547813 1102 18-19 6.946166145640085 1102 20-21 6.230402250285156 1102 22-23 6.347048391825016 1102 24-25 6.199349487099685 1102 26-27 6.419571543512195 1102 28-29 6.278555043342184 1102 30-31 6.506005458640175 1102 32-33 6.277134386653902 1102 34-35 6.245882959645101 1102 36-37 6.33669232023583 1102 38-39 6.139697110866162 1102 40-41 6.383291434247077 1102 42-43 6.353864813609366 1102 44-45 6.136497054505426 1102 46-47 6.011420766090605 1102 48-49 5.779976639621648 1102 50-51 5.8359098076413325 1102 52-53 5.6326732316132215 1102 54-55 5.9233154681606806 1102 56-57 5.80091868975742 1102 58-59 5.986863581804059 1102 60-61 5.719668609868965 1102 62-63 5.743373377069048 1102 64-65 5.836309636361676 1102 66-67 5.629095466937365 1102 68-69 5.577447529536599 1102 70-71 5.384340679353169 1102 72-73 5.229011008711602 1102 74-75 5.294581942138631 1102 76-77 5.2198170814922875 1102 78-79 5.131795343454659 1102 80-81 5.28026579472894 1102 82-83 5.001272312101854 1102 84-85 4.952355835578903 1102 86-87 4.883378067615656 1102 88-89 5.0508606634849365 1102 90-91 5.054835015375872 1102 92-93 5.088139248888204 1102 94-95 4.973477083921043 1102 96-97 4.985008857492186 1102 98-99 4.738168402447943 1102 100-101 4.6541062206726735 1103 1 4.983663440687543 1103 2 5.620864645010315 1103 3 5.281560410542557 1103 4 6.663109764725444 1103 5 6.307693283943905 1103 6 5.715202872917487 1103 7 6.109717003747043 1103 8 5.600808660856753 1103 9 6.2646391935169845 1103 10-11 6.32320654201115 1103 12-13 6.5363734230483 1103 14-15 6.677449130371887 1103 16-17 6.578316075451731 1103 18-19 6.442302762994693 1103 20-21 5.836152555624164 1103 22-23 6.071955435110514 1103 24-25 5.795743925786699 1103 26-27 5.949592064506806 1103 28-29 5.893473429006907 1103 30-31 6.074553604301634 1103 32-33 5.805436914611153 1103 34-35 5.900315327055331 1103 36-37 5.789505206166172 1103 38-39 5.512913601896688 1103 40-41 5.542669598308336 1103 42-43 5.5799272003842155 1103 44-45 5.453559935895406 1103 46-47 5.303005789098858 1103 48-49 5.096591799249243 1103 50-51 5.015388734993355 1103 52-53 4.781878903256878 1103 54-55 5.167223124961907 1103 56-57 5.228498825638962 1103 58-59 5.11922261365239 1103 60-61 4.873564931385268 1103 62-63 4.922693214569669 1103 64-65 4.840688952538514 1103 66-67 4.928622957663684 1103 68-69 4.898178097092568 1103 70-71 4.80871628095047 1103 72-73 4.615293937650474 1103 74-75 4.790468307787197 1103 76-77 4.769370643799604 1103 78-79 4.71421087436995 1103 80-81 4.643081214746012 1103 82-83 4.62558639895661 1103 84-85 4.634469163172472 1103 86-87 4.6108832592300715 1103 88-89 4.452775970503875 1103 90-91 4.397924996453938 1103 92-93 4.311366963113592 1103 94-95 4.273547714849045 1103 96-97 4.156695539092848 1103 98-99 3.9561890721392423 1103 100-101 4.063183195898215 1104 1 4.459131363439205 1104 2 5.33292503747025 1104 3 4.888870065696594 1104 4 6.32067892046577 1104 5 5.896764056427294 1104 6 5.517012492538711 1104 7 6.0745422933394835 1104 8 5.46727301256055 1104 9 6.242877565403425 1104 10-11 6.322000924717329 1104 12-13 6.2731493864317365 1104 14-15 6.242687100293356 1104 16-17 6.254164643479307 1104 18-19 6.375168784296619 1104 20-21 5.756485764592643 1104 22-23 5.958961917628368 1104 24-25 5.744065055819039 1104 26-27 5.885468936641974 1104 28-29 5.7902886339424136 1104 30-31 5.940827244727323 1104 32-33 5.909595253274407 1104 34-35 5.840108859194757 1104 36-37 5.84787035247253 1104 38-39 5.725191077645544 1104 40-41 5.9882466512166985 1104 42-43 5.957807579164015 1104 44-45 5.964205622655882 1104 46-47 5.884565535305736 1104 48-49 5.544110664952971 1104 50-51 5.408268125516752 1104 52-53 5.263220566814422 1104 54-55 5.500610035117191 1104 56-57 5.390166747058142 1104 58-59 5.225448240362404 1104 60-61 5.111061267063764 1104 62-63 5.090780083418046 1104 64-65 5.146667570667358 1104 66-67 5.136928530985646 1104 68-69 5.107001949826824 1104 70-71 5.011261651913053 1104 72-73 5.018841222943443 1104 74-75 4.917636364675236 1104 76-77 4.956534470061911 1104 78-79 4.980630643495484 1104 80-81 4.922715304484136 1104 82-83 4.711114161188785 1104 84-85 4.8402560706627895 1104 86-87 4.742782454855984 1104 88-89 4.804172743235995 1104 90-91 4.88119431520105 1104 92-93 4.7831922171732195 1104 94-95 4.609373897203451 1104 96-97 4.541996446512098 1104 98-99 4.363110031614067 1104 100-101 4.354617511269712 1105 1 4.401295009359348 1105 2 5.05973558680045 1105 3 4.756540533527591 1105 4 6.035642557592478 1105 5 3.562115204470853 1105 6 4.770868865684342 1105 7 5.4492752425593665 1105 8 0.9143597358649664 1105 9 0.5994553084071441 1105 10-11 2.1136283706426724 1105 12-13 5.443619840473023 1105 14-15 3.5207812670449563 1105 16-17 5.719478394421095 1105 18-19 6.164119352172982 1105 20-21 3.0725072625551597 1105 22-23 5.324436148851877 1105 24-25 5.508426092485681 1105 26-27 5.7308832575585775 1105 28-29 3.023738355450174 1105 30-31 5.462328205010948 1105 32-33 5.627708041134664 1105 34-35 5.336082862588228 1105 36-37 5.494440386227357 1105 38-39 5.450143638035488 1105 40-41 5.529434300230527 1105 42-43 2.7942371027394266 1105 44-45 4.963065348300546 1105 46-47 5.2349693840345815 1105 48-49 5.167080179694981 1105 50-51 5.16558529191062 1105 52-53 4.9596826311144575 1105 54-55 5.337281128481543 1105 56-57 5.195998096871175 1105 58-59 5.122240982976415 1105 60-61 4.950682784411825 1105 62-63 4.845334935498396 1105 64-65 4.83815509597348 1105 66-67 4.920016597288182 1105 68-69 4.886179558330877 1105 70-71 4.6035219507301335 1105 72-73 4.558910706109003 1105 74-75 4.657850664807334 1105 76-77 4.555304912311989 1105 78-79 4.6774474431629365 1105 80-81 4.6039277450035065 1105 82-83 4.53133227847329 1105 84-85 4.507039025578422 1105 86-87 4.597720795853601 1105 88-89 4.532160914060558 1105 90-91 4.5326688152240315 1105 92-93 4.477956797796075 1105 94-95 4.433175965860233 1105 96-97 4.445519043654631 1105 98-99 4.449438755386129 1105 100-101 4.6367707749350195 1106 1 4.059275291015311 1106 2 4.2446496356737065 1106 3 4.428563418387284 1106 4 5.848092678487806 1106 5 5.799944425084746 1106 6 4.774745277291366 1106 7 5.3936226416497135 1106 8 5.035283680493269 1106 9 5.7701377263359035 1106 10-11 5.741814231312091 1106 12-13 6.034367511407677 1106 14-15 5.665265421824689 1106 16-17 5.824558739546298 1106 18-19 5.959194526588348 1106 20-21 5.315880941873171 1106 22-23 5.379578763845526 1106 24-25 5.37563146505973 1106 26-27 5.651587694764832 1106 28-29 5.689595888918777 1106 30-31 5.733879798609273 1106 32-33 5.576848048267895 1106 34-35 5.11904178485041 1106 36-37 5.105055465454615 1106 38-39 5.107601430715679 1106 40-41 5.333629947368998 1106 42-43 2.428097731780408 1106 44-45 4.498078592745465 1106 46-47 4.775371447243199 1106 48-49 4.57965040405637 1106 50-51 4.8337465372968715 1106 52-53 4.73589430032202 1106 54-55 4.925070436550172 1106 56-57 4.882487809122058 1106 58-59 4.770339911260439 1106 60-61 4.535753973592659 1106 62-63 4.6270452651651865 1106 64-65 4.690190043044321 1106 66-67 4.862509190614496 1106 68-69 4.848842782303187 1106 70-71 4.845931875677508 1106 72-73 4.389371669842994 1106 74-75 4.51384130214571 1106 76-77 4.545784804752337 1106 78-79 4.608724467716257 1106 80-81 4.61459919913807 1106 82-83 4.672974117304776 1106 84-85 4.609894844831228 1106 86-87 4.717062732959132 1106 88-89 4.700146421995509 1106 90-91 4.80351940393755 1106 92-93 4.7245855329578355 1106 94-95 4.573938376513134 1106 96-97 4.6111450389792275 1106 98-99 4.524831001840521 1106 100-101 4.518541656457227 1107 1 3.3082155518977707 1107 2 0.08966137980592848 1107 3 1.4543504286461442 1107 4 3.162647882827283 1107 5 3.167790076898374 1107 6 4.187665146185402 1107 7 4.988956751608718 1107 8 4.534134232387775 1107 9 5.105685134640986 1107 10-11 2.534441942170325 1107 12-13 5.129212094533845 1107 14-15 3.811703576463433 1107 16-17 2.3931626541367237 1107 18-19 3.2528140744550527 1107 20-21 3.014810660257634 1107 22-23 4.596089037592538 1107 24-25 4.5198753965061265 1107 26-27 3.7866703308044087 1107 28-29 4.508699707408542 1107 30-31 3.9575516769695938 1107 32-33 4.416420028864881 1107 34-35 4.69807856147488 1107 36-37 4.9731490389222905 1107 38-39 4.870209040143354 1107 40-41 3.725475347726743 1107 42-43 4.1885388653892015 1107 44-45 4.764591889377073 1107 46-47 4.798188962167377 1107 48-49 3.1282171807800943 1107 50-51 4.505860712700581 1107 52-53 4.406749767637418 1107 54-55 5.063833321783733 1107 56-57 4.089582925313618 1107 58-59 3.4796824895072795 1107 60-61 4.274742702895299 1107 62-63 4.866720234387408 1107 64-65 5.039550154021786 1107 66-67 5.015757934312681 1107 68-69 4.92544320418358 1107 70-71 4.760995135177261 1107 72-73 4.6869118689443106 1107 74-75 4.773358726443508 1107 76-77 4.60219905002829 1107 78-79 4.522297715819285 1107 80-81 4.736659443874416 1107 82-83 4.639823976793277 1107 84-85 4.543836319379292 1107 86-87 4.441331743820161 1107 88-89 4.510393414225344 1107 90-91 4.6724707397066325 1107 92-93 4.626243878420304 1107 94-95 4.689706584730626 1107 96-97 4.736873727116496 1107 98-99 4.554621980650548 1107 100-101 4.7243115159818245 1108 1 -1.7937074049605677 1108 2 -1.2225190083635766 1108 3 -1.6560603586318692 1108 4 -4.0133082834939025 1108 5 -8.925254970302003 1108 6 -7.284864041552623 1108 7 -5.841082550120586 1108 8 -6.064864835304483 1108 9 -7.229048496590121 1108 10-11 -3.0334654230608393 1108 12-13 -1.6495641846611093 1108 14-15 -6.390167807040335 1108 16-17 -5.343970239852489 1108 18-19 -3.7826778897027786 1108 20-21 -4.1251803052239 1108 22-23 -3.4077278103678417 1108 24-25 -8.212057533998912 1108 26-27 -9.204224847270563 1108 28-29 -6.088650377034295 1108 30-31 -6.050042014187326 1108 32-33 -2.5011849790495013 1108 34-35 -0.8605271059278756 1108 36-37 -4.690591508804577 1108 38-39 -5.437156086090699 1108 40-41 -3.1608898745187375 1108 42-43 -4.017844399660593 1108 44-45 -1.7091206523918387 1108 46-47 -2.274373121291216 1108 48-49 -1.1449012443385946 1108 50-51 -0.529883347051058 1108 52-53 -2.151799499606472 1108 54-55 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2114 82-83 -21.477565084094234 2114 84-85 -21.183459862251258 2114 86-87 -20.905933478058063 2114 88-89 -20.573971910852055 2114 90-91 -20.235681995071488 2114 92-93 -19.91400379959827 2114 94-95 -19.676134488540786 2114 96-97 -19.310597893110543 2114 98-99 -19.023551393962453 2114 100-101 -18.446483117661106 2201 1 5.627164194911636 2201 2 6.193693859429846 2201 3 5.79048947279793 2201 4 7.087967990385774 2201 5 6.647546276212559 2201 6 6.191416090749719 2201 7 6.694565977518515 2201 8 6.1886095259056155 2201 9 7.054705974233478 2201 10-11 7.047403158667407 2201 12-13 7.080599499521785 2201 14-15 7.313048191549786 2201 16-17 7.1199998752459095 2201 18-19 7.25854371725309 2201 20-21 6.561926679800674 2201 22-23 6.657008013655368 2201 24-25 6.4704253458169525 2201 26-27 6.678126569159691 2201 28-29 6.491898780061941 2201 30-31 6.5860845885091095 2201 32-33 6.4056738190379825 2201 34-35 6.185821718170004 2201 36-37 6.281035233850364 2201 38-39 6.037128483199847 2201 40-41 6.176959418262065 2201 42-43 6.258672263844584 2201 44-45 6.103992391609481 2201 46-47 6.101559858500675 2201 48-49 5.708035074670779 2201 50-51 5.8408802037953045 2201 52-53 5.564263331607787 2201 54-55 5.592203321082167 2201 56-57 5.555309499449134 2201 58-59 5.459186581860635 2201 60-61 5.126790861081904 2201 62-63 4.999630943805418 2201 64-65 5.022337393955311 2201 66-67 4.968527844068298 2201 68-69 4.939511790129963 2201 70-71 4.585780529628927 2201 72-73 4.53589618449065 2201 74-75 4.36185724004336 2201 76-77 4.152199773011606 2201 78-79 4.240542681847099 2201 80-81 4.146185311414229 2201 82-83 3.876871864530571 2201 84-85 3.687064268521965 2201 86-87 3.68410284783711 2201 88-89 3.6386643112548462 2201 90-91 3.4777326390748513 2201 92-93 2.1413931905418444 2201 94-95 2.836709310109967 2201 96-97 3.1668281577457087 2201 98-99 3.0315861254978493 2201 100-101 2.8037763530187085 2202 1 6.0538003553393125 2202 2 6.822448024038739 2202 3 6.437410259807592 2202 4 7.738820107662203 2202 5 7.176469583135749 2202 6 6.736735336239825 2202 7 7.261582260690737 2202 8 6.713764131088656 2202 9 7.753443798170117 2202 10-11 7.807673434741048 2202 12-13 7.880555675565258 2202 14-15 8.166473593621266 2202 16-17 8.080439279222809 2202 18-19 8.210541245184196 2202 20-21 7.526561352144906 2202 22-23 7.607341931740454 2202 24-25 7.406571816403247 2202 26-27 7.670773158564959 2202 28-29 7.572533455000158 2202 30-31 7.639743571836554 2202 32-33 7.51224100681247 2202 34-35 7.3305491456979865 2202 36-37 7.4209878172228905 2202 38-39 7.3053226667044875 2202 40-41 7.430069932019784 2202 42-43 7.484408476771659 2202 44-45 7.4149998857798565 2202 46-47 7.406254202616292 2202 48-49 7.0903740922446055 2202 50-51 7.141951793924722 2202 52-53 6.914446503064365 2202 54-55 7.149496947499436 2202 56-57 7.133147965296491 2202 58-59 6.987557982182317 2202 60-61 5.372262841923298 2202 62-63 5.264380169464495 2202 64-65 6.3464858062844165 2202 66-67 6.47950145069057 2202 68-69 6.457032798176073 2202 70-71 6.176098267004246 2202 72-73 5.993790347546032 2202 74-75 5.997786911023585 2202 76-77 5.727382285163351 2202 78-79 5.7566635137615485 2202 80-81 5.751838191092034 2202 82-83 5.624073349674166 2202 84-85 5.49274119301117 2202 86-87 5.406063189534272 2202 88-89 5.36243464288302 2202 90-91 5.320830084906298 2202 92-93 5.288023415064323 2202 94-95 5.121977147115977 2202 96-97 4.988346844462352 2202 98-99 4.821352799317193 2202 100-101 4.722358870675468 2203 1 5.431777503234606 2203 2 6.212012528947991 2203 3 5.706880694771112 2203 4 7.090726389653913 2203 5 6.490878946871341 2203 6 6.120369145330258 2203 7 6.624035667930933 2203 8 5.98324556409727 2203 9 6.457283857266283 2203 10-11 6.613372805011309 2203 12-13 6.778525894692141 2203 14-15 6.873244631735904 2203 16-17 6.97861891945842 2203 18-19 7.045889512029767 2203 20-21 6.422910734375545 2203 22-23 6.472952694427381 2203 24-25 6.348605244961316 2203 26-27 6.465201365617819 2203 28-29 6.3986094533247915 2203 30-31 6.463101678266469 2203 32-33 6.389736990234756 2203 34-35 6.225842715599271 2203 36-37 6.401974030256856 2203 38-39 6.058577400675521 2203 40-41 6.278497165745655 2203 42-43 6.325132954662376 2203 44-45 6.233064266502851 2203 46-47 6.3019097915020375 2203 48-49 5.8002273324978155 2203 50-51 5.70850325606899 2203 52-53 5.6637992222851885 2203 54-55 5.750777486550291 2203 56-57 5.604845870993753 2203 58-59 5.5429766731766605 2203 60-61 5.365276792027004 2203 62-63 5.378813912200059 2203 64-65 5.374756281207496 2203 66-67 5.3626971709846245 2203 68-69 5.147709113794306 2203 70-71 4.920783690836828 2203 72-73 4.886505792375711 2203 74-75 4.81571931520541 2203 76-77 4.717615104613028 2203 78-79 4.683398661589024 2203 80-81 4.645528795920363 2203 82-83 4.474453194656718 2203 84-85 4.4683553192668946 2203 86-87 4.48782128203333 2203 88-89 4.433644235377951 2203 90-91 4.290339837881035 2203 92-93 4.212297292049357 2203 94-95 4.0086839933074 2203 96-97 4.135581340197401 2203 98-99 3.8285684334049 2203 100-101 3.7562333078275962 2204 1 5.188316058919803 2204 2 5.861272633002859 2204 3 5.497290259040543 2204 4 6.800194434628782 2204 5 6.328062881672459 2204 6 6.045820124423841 2204 7 6.394139144932183 2204 8 5.7344617775934985 2204 9 6.546955830562375 2204 10-11 6.818964309469649 2204 12-13 6.867703143127191 2204 14-15 6.874105425074735 2204 16-17 6.861846191624949 2204 18-19 6.9260204279346524 2204 20-21 6.314137622163674 2204 22-23 6.331760056572218 2204 24-25 6.234985101715441 2204 26-27 6.495960499961317 2204 28-29 6.306996921030834 2204 30-31 6.339980895796721 2204 32-33 6.251029891116421 2204 34-35 6.089035172952421 2204 36-37 6.078064460468479 2204 38-39 6.007183509564985 2204 40-41 6.271980036558801 2204 42-43 6.198257535465924 2204 44-45 6.034823414481394 2204 46-47 5.966829873909649 2204 48-49 5.816777776643153 2204 50-51 5.790903562792561 2204 52-53 5.585697136058467 2204 54-55 5.952097780885445 2204 56-57 5.721343592967411 2204 58-59 5.606695995138367 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6.621083676025247 2205 22-23 6.594548427933507 2205 24-25 6.440546218807103 2205 26-27 6.719164790419779 2205 28-29 6.583223068326216 2205 30-31 6.793090327781037 2205 32-33 6.488530567262078 2205 34-35 6.436488713174896 2205 36-37 6.513654253754563 2205 38-39 6.33615008473296 2205 40-41 6.641690806206832 2205 42-43 6.536104969578869 2205 44-45 6.368346716174912 2205 46-47 6.448442902227011 2205 48-49 6.164746368439356 2205 50-51 6.146417711568983 2205 52-53 5.830582275100678 2205 54-55 6.038051511952418 2205 56-57 6.02056029250809 2205 58-59 5.943842218588699 2205 60-61 5.864871595805173 2205 62-63 5.75252813451938 2205 64-65 5.740562824740795 2205 66-67 5.622504292138068 2205 68-69 5.677837812372559 2205 70-71 5.42188947268744 2205 72-73 5.436891579562392 2205 74-75 5.49856694622547 2205 76-77 5.339515189032689 2205 78-79 5.211756044656678 2205 80-81 5.065399768375414 2205 82-83 4.963304740153607 2205 84-85 4.877755508615589 2205 86-87 4.883461815358679 2205 88-89 4.886111496952534 2205 90-91 4.7342316182737605 2205 92-93 4.7330024559870765 2205 94-95 4.658986154890851 2205 96-97 4.665124478054185 2205 98-99 4.47585283600478 2205 100-101 4.225098651775891 2206 1 5.21326267579683 2206 2 5.685187926013043 2206 3 5.3002952876096 2206 4 6.762798562000736 2206 5 6.264720215665655 2206 6 5.947827052982163 2206 7 6.412879130189097 2206 8 5.907955715970171 2206 9 6.5850954330527784 2206 10-11 6.746773196158998 2206 12-13 6.786945120085068 2206 14-15 6.954162286320145 2206 16-17 6.972283673671953 2206 18-19 6.949324713466012 2206 20-21 6.3034305423675825 2206 22-23 6.383713515487113 2206 24-25 6.122464033983764 2206 26-27 6.35919086241282 2206 28-29 6.192505955497129 2206 30-31 6.371000037582988 2206 32-33 6.113421178160156 2206 34-35 6.009764436594104 2206 36-37 6.308724196269605 2206 38-39 5.912780864115451 2206 40-41 6.1094653713410665 2206 42-43 6.2492687865863665 2206 44-45 6.111817451491383 2206 46-47 5.965419244352187 2206 48-49 5.69437399604519 2206 50-51 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54-55 -5.271351213234837 2313 56-57 -4.59284023385781 2313 58-59 -5.67558971161408 2313 60-61 -3.773279490246747 2313 62-63 -5.808572074614325 2313 64-65 -5.983512733640065 2313 66-67 -7.122994419586011 2313 68-69 -8.284665527317706 2313 70-71 -6.8433264632396416 2313 72-73 -6.497764117565325 2313 74-75 -7.685688755815807 2313 76-77 -6.96489105242615 2313 78-79 -7.87192047064141 2313 80-81 -10.177507036608175 2313 82-83 -8.864163022238564 2313 84-85 -8.260779449880124 2313 86-87 -7.2976860553776515 2313 88-89 -8.635827580955148 2313 90-91 -8.364547974452933 2313 92-93 -7.465550191350847 2313 94-95 -7.985412839056249 2313 96-97 -8.063175212698173 2313 98-99 -7.89468541458101 2313 100-101 -7.73514291147554 2314 1 -14.332372404479658 2314 2 -16.49857391431172 2314 3 -16.809476866140905 2314 4 -25.254792777647435 2314 5 -20.424684750870572 2314 6 -19.120537793596483 2314 7 -17.864193612377555 2314 8 -15.609438132546819 2314 9 -17.141825533922315 2314 10-11 -16.37863367214368 2314 12-13 -15.636686156658527 2314 14-15 -18.20087556614667 2314 16-17 -17.586608913096143 2314 18-19 -16.353495021018134 2314 20-21 -18.00257282229613 2314 22-23 -17.136004511064474 2314 24-25 -18.434082431290715 2314 26-27 -18.461335414879354 2314 28-29 -18.755085749875363 2314 30-31 -18.850846504493596 2314 32-33 -16.423398117454813 2314 34-35 -17.07243576089703 2314 36-37 -17.025567944314822 2314 38-39 -15.888855752203174 2314 40-41 -19.10772550017998 2314 42-43 -19.728392772130675 2314 44-45 -15.812598303912196 2314 46-47 -15.271937666008785 2314 48-49 -14.517203007209753 2314 50-51 -13.870809300966336 2314 52-53 -14.235288555515456 2314 54-55 -14.321922402981286 2314 56-57 -13.023043633133373 2314 58-59 -12.350568814428234 2314 60-61 -12.536514374615649 2314 62-63 -14.883035713510955 2314 64-65 -12.919288715807799 2314 66-67 -14.324025347421063 2314 68-69 -17.3597560817897 2314 70-71 -16.532306680570375 2314 72-73 -14.89474099134632 2314 74-75 -13.33378014617524 2314 76-77 -13.988713844290178 2314 78-79 -14.07880261051324 2314 80-81 -13.67374554315596 2314 82-83 -14.227565084094234 2314 84-85 -14.440216619008014 2314 86-87 -14.716744288868874 2314 88-89 -13.95910704598719 2314 90-91 -13.64784415723365 2314 92-93 -13.589679475273945 2314 94-95 -13.351810164216461 2314 96-97 -13.188976271488922 2314 98-99 -13.205983826394885 2314 100-101 -12.662699333877322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59426.0 3 10384.0 4 7073.0 5 5885.0 6 6399.0 7 7616.0 8 9208.0 9 10383.0 10 11378.0 11 12423.0 12 13990.0 13 14532.0 14 16332.0 15 18693.0 16 21207.0 17 22776.0 18 20100.0 19 17176.0 20 16956.0 21 17363.0 22 19917.0 23 24517.0 24 30244.0 25 36893.0 26 43989.0 27 50641.0 28 56969.0 29 63561.0 30 72255.0 31 81728.0 32 95017.0 33 114627.0 34 139736.0 35 171031.0 36 210945.0 37 247948.0 38 214412.0 39 52614.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.86684462435771 21.401612374904055 14.222584004341385 22.508958996396846 2 14.886493833399983 20.924732396301266 41.07321166146139 23.115562108837363 3 17.382514718645183 27.678969342997206 30.78263903548894 24.15587690286867 4 11.340808997133854 18.018295574204586 40.78560804506036 29.855287383601205 5 11.913748663617534 38.73919936059333 35.6412407506172 13.705811225171935 6 28.092320711017194 39.39933329909338 18.79514477388176 13.71320121600767 7 25.82653876390121 33.21819214473252 23.62822425087701 17.327044840489258 8 25.412880794750585 34.91666067384729 21.06899178274391 18.60146674865822 9 26.27687484926505 15.77911807885034 22.116565752725702 35.82744131915891 10-11 23.72090279943006 26.89592823967229 29.16663199582188 20.216536965075775 12-13 24.78107254191901 25.13632233213113 28.71134779194429 21.37125733400557 14-15 22.546308257196763 26.144103624022357 26.82576077851109 24.483827340269787 16-17 20.821055253134567 29.00354709608572 27.772032716756584 22.40336493402313 18-19 20.55491837664763 28.095306626644323 30.405133198130656 20.944641798577386 20-21 21.893523544055856 27.324870645522502 30.078554380245663 20.70305143017598 22-23 21.95954225770678 27.046796141486652 29.76528306575606 21.22837853505051 24-25 21.447117089575055 27.52628553959034 30.029104249464993 20.99749312136961 26-27 21.18473023285751 27.548609773569215 29.949699723033994 21.31696027053928 28-29 20.87523993073655 27.34817799405552 30.363740744917212 21.412841330290725 30-31 21.366313989850777 27.202411504998526 30.124876266267652 21.306398238883048 32-33 21.37644311976555 27.7014179640742 29.64286989628077 21.279269019879475 34-35 21.6366600016004 27.50312554983126 30.042841261222065 20.817373187346277 36-37 21.606547076687587 26.98626108249516 29.84649661518406 21.560695225633193 38-39 21.442955786945685 27.633091664695105 29.98969729724704 20.934255251112173 40-41 21.255865182797535 27.036942549276343 30.417009818945235 21.29018244898089 42-43 21.256357221549294 27.225467826930945 29.837627902699044 21.680547048820724 44-45 21.29953216059633 27.69085915661737 29.731597623874727 21.27801105891157 46-47 21.469660251457796 27.415009059944822 29.411669756757174 21.70366093184021 48-49 21.673898783624942 27.467125295876006 29.28077978705243 21.57819613344662 50-51 21.47518148822546 27.609099574836044 28.789243787240977 22.126475149697526 52-53 21.268858664536676 28.13358497376925 28.693221629371006 21.90433473232307 54-55 21.431334053926868 28.026161957073214 28.580504268413737 21.96199972058618 56-57 21.637231350285127 27.98011893435174 28.356088184107257 22.026561531255876 58-59 21.201266142458188 27.86636361630293 28.948052362002976 21.984317879235903 60-61 21.592474055408342 28.429100038449125 28.323444493148298 21.654981412994236 62-63 21.5347901469128 27.950570371934102 28.50774830139407 22.006891179759027 64-65 21.213345100430807 27.807219954808094 28.660725455242027 22.318709489519073 66-67 21.52831531130032 27.771522842242707 28.345755244372512 22.354406602084456 68-69 21.280145857278008 27.800793985879483 28.453897215771818 22.465162941070687 70-71 21.553701553569613 27.559661334334123 28.646212050133308 22.240425061962952 72-73 21.837250486712172 27.5949186467447 28.555930851687663 22.01190001485547 74-75 21.59665536868936 28.068876613577903 28.30008932829811 22.034378689434632 76-77 21.50253813556009 27.904318055653288 28.413533334897068 22.179610473889554 78-79 21.351784415828114 27.91538635954933 28.289998358079423 22.442830866543133 80-81 21.251338947137977 28.067630513139274 28.33732574140533 22.343704798317425 82-83 21.84900292418236 27.673276374326615 28.59211663109172 21.8856040703993 84-85 21.708877708191622 27.89549761921516 28.015831828239467 22.379792844353748 86-87 21.13625790662945 28.222953267812883 28.462619918842215 22.178168906715456 88-89 21.85208152526603 27.488267109203356 28.307174997458933 22.35247636807168 90-91 21.580846996458142 27.509548550027755 28.692904166568933 22.21670028694517 92-93 21.74567432114403 27.846704235373227 28.344313677198414 22.063307766284336 94-95 21.122233168359408 27.42816108804604 28.669374858286616 22.78023088530794 96-97 21.501316468463397 27.435051290471385 28.370750553170858 22.692881687894353 98-99 21.096211659199838 27.714886355639994 28.357312215107232 22.831589770052933 100-101 21.292118194825605 27.537940093354912 28.287432857176363 22.882508854643117 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 673.0 1 443.0 2 273.5 3 334.0 4 281.0 5 268.5 6 441.5 7 852.0 8 1524.0 9 1930.5 10 1713.0 11 1496.0 12 1586.5 13 1752.0 14 1901.0 15 2174.5 16 2415.0 17 2585.0 18 2848.5 19 3095.0 20 3440.0 21 3916.5 22 4432.5 23 5241.0 24 6214.0 25 7604.0 26 10383.5 27 13605.5 28 16215.0 29 20753.5 30 27457.0 31 33600.0 32 41781.5 33 51162.5 34 59652.0 35 67203.5 36 75319.5 37 83920.5 38 84143.5 39 79876.5 40 75287.5 41 69697.0 42 65719.0 43 65511.5 44 67708.0 45 69665.5 46 69646.5 47 68481.5 48 68309.5 49 66361.0 50 64421.5 51 63267.5 52 61194.0 53 59131.5 54 56742.5 55 52321.5 56 47238.0 57 41186.5 58 35854.0 59 32530.5 60 27899.0 61 22517.0 62 17810.0 63 13719.5 64 10237.0 65 7261.5 66 5310.5 67 3953.0 68 3044.0 69 2388.0 70 1804.0 71 1353.5 72 1027.5 73 796.5 74 616.5 75 494.0 76 403.0 77 307.0 78 222.0 79 168.5 80 125.0 81 98.0 82 82.5 83 64.0 84 50.0 85 41.5 86 35.0 87 24.0 88 19.0 89 19.5 90 15.5 91 8.0 92 4.5 93 4.5 94 3.5 95 2.0 96 1.0 97 2.5 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04661881640982338 2 0.4099426109664657 3 0.0 4 0.5496035934604648 5 0.21946027725001757 6 0.09719583421293365 7 0.10882610497345561 8 0.11097623906363614 9 0.10911930507666205 10-11 0.16424092447947208 12-13 0.21699250971469677 14-15 0.13135364623648346 16-17 0.12702894471418855 18-19 0.17225506063378135 20-21 0.10726237108968795 22-23 0.10638277078006864 24-25 0.09817316789028842 26-27 0.16387442435046404 28-29 0.11261327297320542 30-31 0.1310115794494093 32-33 0.1033530363802688 34-35 0.15361242073823875 36-37 0.1390745822875863 38-39 0.1341634805588785 40-41 0.10865507157991852 42-43 0.1100477720701491 44-45 0.0918204989874823 46-47 0.09507013346468697 48-49 0.09924823493537871 50-51 0.08605423029108906 52-53 0.09443486657440636 54-55 0.31269791006966435 56-57 0.33500555125528736 58-59 0.3478819224544367 60-61 0.16734395890507353 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2046384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.433977377737904 #Duplication Level Percentage of deduplicated Percentage of total 1 79.20146781074503 37.56840632418517 2 11.269145273266705 10.6908076391715 3 3.758883255763412 5.348963499583185 4 1.6847344348437463 3.1965462027949725 5 0.9127727230218285 2.164822034741682 6 0.5568982414901937 1.5849539153128724 7 0.39068924211475997 1.2972361270537962 8 0.2841817807386585 1.078389772697824 9 0.18818118399575834 0.8033563822113663 >10 1.4132451813357068 13.398689946107545 >50 0.19105771023875806 6.4032492504815215 >100 0.13933654971900317 12.121073239164119 >500 0.007525290181189307 2.3390489823229546 >1k 0.0016599904811447001 1.2815189480340736 >5k 2.2133206415262665E-4 0.7229377361375194 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7901 0.3860956692390089 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5871 0.2868963009874979 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3451 0.16863892602756864 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 2460 0.12021204231463889 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.4433341933869695E-5 0.0 14-15 0.0 0.0 0.0 4.886668386773939E-5 0.0 16-17 0.0 0.0 0.0 4.886668386773939E-5 0.0 18-19 0.0 0.0 0.0 4.886668386773939E-5 0.0 20-21 0.0 0.0 0.0 1.7103339353708786E-4 0.0 22-23 0.0 0.0 0.0 3.665001290080454E-4 4.886668386773939E-5 24-25 0.0 0.0 0.0 8.307336257515697E-4 4.886668386773939E-5 26-27 0.0 0.0 0.0 0.0022723007998498813 4.886668386773939E-5 28-29 0.0 0.0 0.0 0.004691201651302981 4.886668386773939E-5 30-31 0.0 0.0 0.0 0.011947904205662281 4.886668386773939E-5 32-33 0.0 0.0 0.0 0.02890464350776785 4.886668386773939E-5 34-35 0.0 0.0 0.0 0.05429088577705846 4.886668386773939E-5 36-37 0.0 0.0 0.0 0.09485023338728216 4.886668386773939E-5 38-39 4.886668386773939E-5 0.0 0.0 0.15353912071243714 4.886668386773939E-5 40-41 4.886668386773939E-5 0.0 0.0 0.21638167616635 4.886668386773939E-5 42-43 7.330002580160908E-5 0.0 0.0 0.27763606439456134 4.886668386773939E-5 44-45 9.773336773547878E-5 0.0 0.0 0.3506184567510301 4.886668386773939E-5 46-47 9.773336773547878E-5 0.0 0.0 0.4416815221385625 4.886668386773939E-5 48-49 9.773336773547878E-5 0.0 0.0 0.5371425890741914 4.886668386773939E-5 50-51 9.773336773547878E-5 0.0 0.0 0.635657823751554 4.886668386773939E-5 52-53 9.773336773547878E-5 0.0 0.0 0.7349060586869327 4.886668386773939E-5 54-55 9.773336773547878E-5 0.0 0.0 0.8404336624993158 4.886668386773939E-5 56-57 9.773336773547878E-5 0.0 0.0 0.9678046740005786 4.886668386773939E-5 58-59 9.773336773547878E-5 0.0 0.0 1.1084918568558004 4.886668386773939E-5 60-61 9.773336773547878E-5 0.0 0.0 1.2602962102909325 4.886668386773939E-5 62-63 9.773336773547878E-5 0.0 0.0 1.4091441293520668 4.886668386773939E-5 64-65 9.773336773547878E-5 0.0 0.0 1.568302918709294 4.886668386773939E-5 66-67 9.773336773547878E-5 0.0 0.0 1.7480590153167732 4.886668386773939E-5 68-69 9.773336773547878E-5 0.0 0.0 1.945407118116639 4.886668386773939E-5 70-71 9.773336773547878E-5 0.0 0.0 2.16166662757332 4.886668386773939E-5 72-73 9.773336773547878E-5 0.0 0.0 2.3782682038170746 4.886668386773939E-5 74-75 9.773336773547878E-5 0.0 0.0 2.5962624805510597 4.886668386773939E-5 76-77 9.773336773547878E-5 0.0 0.0 2.8383968991157085 4.886668386773939E-5 78-79 9.773336773547878E-5 0.0 0.0 3.0967794900663805 4.886668386773939E-5 80-81 9.773336773547878E-5 0.0 0.0 3.377762922305882 4.886668386773939E-5 82-83 9.773336773547878E-5 0.0 0.0 3.6726000594218875 4.886668386773939E-5 84-85 9.773336773547878E-5 0.0 0.0 3.9674616298798266 4.886668386773939E-5 86-87 9.773336773547878E-5 2.4433341933869695E-5 0.0 4.275492771640122 4.886668386773939E-5 88-89 9.773336773547878E-5 4.886668386773939E-5 0.0 4.6049275209344875 4.886668386773939E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3515 0.0 57.365154 1 GTATCAA 7310 0.0 45.470894 1 ATCAACG 9135 0.0 35.57598 3 CAACGCA 9145 0.0 35.48714 5 TCAACGC 9825 0.0 33.90741 4 TATCAAC 9895 0.0 33.30152 2 AACGCAG 9955 0.0 33.056614 6 CGCAGAG 10610 0.0 29.905634 8 ACGCAGA 10850 0.0 29.420225 7 GCAGAGT 11085 0.0 27.379625 9 GTACATG 8865 0.0 24.312252 1 GTACTAG 1070 0.0 24.17138 1 GAGTACT 6170 0.0 23.556812 12-13 GTGGTAT 1835 0.0 22.968784 1 GCATCGT 920 0.0 22.751364 8 CAGAGTA 11145 0.0 22.705524 10-11 TACATGG 9255 0.0 22.666742 2 ACATGGG 9230 0.0 22.594257 3 TGGTATC 1855 0.0 22.462948 2 CTGTGCG 1005 0.0 22.247149 9 >>END_MODULE