FastQCFastQC Report
Sat 18 Jun 2016
SRR1931119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2046384
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97610.4769877012330042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79760.38976067052908936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52760.257820624086193No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA30000.14660005160321818No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC23380.11425030688277468No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA23380.11425030688277468No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC21680.10594297062525901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37050.057.619831
GTATCAA76550.045.8379331
ATCAACG99500.034.553163
CAACGCA100500.034.1612665
TCAACGC105450.032.87374
TATCAAC106300.032.6555022
AACGCAG108800.032.1225936
GTGGTAT17150.028.832551
CGCAGAG120900.028.0828658
ACGCAGA122700.027.9417957
GTGATCG5850.025.9750148
GTACATG94200.025.8424021
GCAGAGT130600.025.4516839
TAGTACT11850.024.8453794
TGGTATC19150.024.7972492
ACATGGG95750.024.3013043
TACATGG99000.024.2230322
GTACTAG10400.023.7729911
GCTATTG14950.023.1869358
TGATCGC6400.023.0008249
Produced by FastQC (version 0.11.5)