##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931119_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2046384 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.281489202417532 31.0 31.0 34.0 30.0 34.0 2 31.279914717863313 31.0 31.0 34.0 28.0 34.0 3 31.41738989358791 31.0 31.0 34.0 28.0 34.0 4 35.09217136177765 35.0 35.0 37.0 32.0 37.0 5 34.972704536391994 35.0 35.0 37.0 32.0 37.0 6 35.08898965199102 36.0 35.0 37.0 32.0 37.0 7 34.93660867168625 36.0 35.0 37.0 32.0 37.0 8 34.99541679372005 36.0 35.0 37.0 32.0 37.0 9 36.35048309603672 38.0 35.0 39.0 32.0 39.0 10-11 36.235222470464976 38.0 35.0 39.0 31.5 39.0 12-13 36.16840925261339 38.0 35.0 39.0 31.5 39.0 14-15 37.060114572827 39.0 36.0 40.0 31.0 41.0 16-17 37.11230516853142 39.0 36.0 40.0 31.0 41.0 18-19 37.20031333317696 39.0 36.0 40.0 31.0 41.0 20-21 37.202421686252436 39.0 36.0 40.0 31.0 41.0 22-23 37.07149953283451 39.0 36.0 40.0 31.0 41.0 24-25 36.918153435523344 39.0 36.0 40.0 31.0 41.0 26-27 36.651803131768034 39.0 35.0 40.0 30.0 41.0 28-29 36.3875751080931 38.0 35.0 40.0 30.0 41.0 30-31 36.133301472255454 38.0 35.0 40.0 29.5 41.0 32-33 35.94089696752907 38.0 34.0 40.0 29.5 41.0 34-35 35.71093426258219 38.0 34.0 40.0 28.5 41.0 36-37 35.93939920366852 38.0 35.0 40.0 30.0 41.0 38-39 35.902585731710175 38.0 35.0 40.0 29.0 41.0 40-41 35.739333135911934 38.0 34.5 40.0 29.0 41.0 42-43 35.53462009085294 38.0 34.0 40.0 27.5 41.0 44-45 35.23171310956302 38.0 34.0 40.0 27.0 41.0 46-47 34.93598928646823 38.0 33.5 40.0 26.0 41.0 48-49 34.605052863978614 38.0 33.0 40.0 24.5 41.0 50-51 34.21803068241347 38.0 33.0 40.0 24.0 41.0 52-53 34.06702529925957 37.5 33.0 40.0 24.0 41.0 54-55 33.79398710115012 37.0 33.0 40.0 23.0 41.0 56-57 33.54746078937286 37.0 32.0 40.0 22.5 41.0 58-59 33.27603445883079 36.5 32.0 40.0 22.0 41.0 60-61 32.98111131635119 36.0 31.5 39.0 21.5 41.0 62-63 32.69785827097944 36.0 31.0 39.0 20.0 41.0 64-65 32.43977449980062 35.5 31.0 39.0 20.0 40.5 66-67 32.18501879412662 35.0 31.0 39.0 19.0 40.0 68-69 31.857078632358345 35.0 31.0 38.5 18.0 40.0 70-71 31.047815072830907 35.0 29.5 38.0 10.5 40.0 72-73 31.02723780092104 34.5 30.0 37.0 13.5 39.5 74-75 30.719957740091793 34.0 30.0 37.0 12.0 39.0 76-77 30.017958750654813 34.0 29.0 36.0 9.0 39.0 78-79 30.05444896949937 34.0 29.0 36.0 7.5 38.5 80-81 29.787970146365492 34.0 29.0 35.5 7.0 37.5 82-83 29.440819269501716 34.0 29.0 35.0 3.5 37.0 84-85 29.087223365702627 34.0 29.0 35.0 2.0 36.5 86-87 28.766141154348354 34.0 29.0 35.0 2.0 36.0 88-89 28.4679375913807 34.0 29.0 35.0 2.0 36.0 90-91 28.160245095739608 33.0 27.5 35.0 2.0 35.5 92-93 27.809521575618263 33.0 27.0 35.0 2.0 35.0 94-95 27.428522701506658 33.0 26.5 35.0 2.0 35.0 96-97 27.053987668003657 33.0 26.0 35.0 2.0 35.0 98-99 26.576629557306937 33.0 25.0 35.0 2.0 35.0 100-101 25.897399266217874 32.5 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7056770879380103 1101 2 0.6272914496354822 1101 3 0.4601054069545256 1101 4 0.8002022140277774 1101 5 0.8185657287731587 1101 6 0.8733183850186634 1101 7 0.849010767861273 1101 8 0.803440632229119 1101 9 1.1629552107767864 1101 10-11 0.9760825542357594 1101 12-13 1.0613411347303199 1101 14-15 1.428925088540673 1101 16-17 1.0277164498089917 1101 18-19 0.8961327366246081 1101 20-21 0.8300659940816502 1101 22-23 0.6645723513082231 1101 24-25 0.6029079408342284 1101 26-27 0.9706097632486319 1101 28-29 0.8897417412668958 1101 30-31 1.1198851902633606 1101 32-33 1.0586616551734025 1101 34-35 0.9429260806107607 1101 36-37 1.020766925707079 1101 38-39 0.923743671521791 1101 40-41 0.9612657943293712 1101 42-43 0.9923906846260522 1101 44-45 0.8499201469310833 1101 46-47 0.9347752417041377 1101 48-49 0.773404783614069 1101 50-51 0.7786654563216402 1101 52-53 0.6744799874852916 1101 54-55 0.6741670616945825 1101 56-57 0.4521416430475682 1101 58-59 0.648440452730803 1101 60-61 0.7278510891311143 1101 62-63 0.6086790494920251 1101 64-65 0.59839720791582 1101 66-67 0.3261777256813829 1101 68-69 0.30239933588369894 1101 70-71 0.535781731737508 1101 72-73 -0.04793013295489956 1101 74-75 -0.2731945317622184 1101 76-77 -0.2372516067448771 1101 78-79 -0.02482370460858263 1101 80-81 -0.0939349520149726 1101 82-83 -0.2171750959415597 1101 84-85 -0.20717019199187092 1101 86-87 -0.2222310755561061 1101 88-89 -0.2595122040071054 1101 90-91 -0.21747355621700137 1101 92-93 -0.3222248741786373 1101 94-95 -0.3898247130221293 1101 96-97 -0.47071792035952953 1101 98-99 -0.5385323430701057 1101 100-101 -0.7568378299451624 1102 1 0.9145390610452608 1102 2 0.8279970931153038 1102 3 0.668011761780452 1102 4 0.9416014998018412 1102 5 1.1576836915312754 1102 6 1.0877012171359368 1102 7 1.2103467487357875 1102 8 1.0882244319687118 1102 9 1.4720166761503393 1102 10-11 1.2255799386530697 1102 12-13 1.3903489041364807 1102 14-15 1.780536765930897 1102 16-17 1.436154197116025 1102 18-19 1.2771815701016749 1102 20-21 1.2413808966087245 1102 22-23 0.9095743165772134 1102 24-25 0.5816117529143057 1102 26-27 1.0029205460529766 1102 28-29 1.2025680119163056 1102 30-31 1.549772989585513 1102 32-33 1.6618766627286732 1102 34-35 1.5799254662949025 1102 36-37 1.7606924450777086 1102 38-39 1.5558880159310107 1102 40-41 1.4928593288011243 1102 42-43 1.642916067960428 1102 44-45 1.5284611988126215 1102 46-47 1.5737089596038558 1102 48-49 1.4232682341302763 1102 50-51 1.3601623169523478 1102 52-53 1.2790742118327572 1102 54-55 1.0941272231189387 1102 56-57 1.1962985141397482 1102 58-59 1.188163051128619 1102 60-61 1.4207247757262493 1102 62-63 1.3918730628136586 1102 64-65 1.3268652518722668 1102 66-67 0.9497127377645498 1102 68-69 0.9816070608948806 1102 70-71 1.3049776765400374 1102 72-73 0.8377635285857181 1102 74-75 0.8162499778664589 1102 76-77 0.7657871467627722 1102 78-79 0.8081202341155098 1102 80-81 0.6274449719033832 1102 82-83 0.6354915324224386 1102 84-85 0.6265729347346927 1102 86-87 0.7912323713674461 1102 88-89 0.8520837098871681 1102 90-91 0.7696703269919887 1102 92-93 0.6206489193408089 1102 94-95 0.65194003294636 1102 96-97 0.6737536931583854 1102 98-99 0.5243807857678497 1102 100-101 0.5948704713881021 1103 1 0.717750483902325 1103 2 0.49250103641532306 1103 3 0.4002848602789584 1103 4 0.6471812012279656 1103 5 0.8330605127819624 1103 6 0.7710277068270557 1103 7 0.7539195613053167 1103 8 0.6501168754526176 1103 9 1.0079438113667152 1103 10-11 0.9597983672809249 1103 12-13 1.0732654137824653 1103 14-15 1.3844420901237413 1103 16-17 1.102329098192996 1103 18-19 0.974764142168489 1103 20-21 0.9500353419587171 1103 22-23 0.7884901261569155 1103 24-25 0.5658843820534187 1103 26-27 0.7751850732088528 1103 28-29 0.8221194062031714 1103 30-31 1.2787211988687233 1103 32-33 1.314997150748944 1103 34-35 1.1636460305931706 1103 36-37 1.3887589241330431 1103 38-39 1.165800799930338 1103 40-41 1.2043601168368312 1103 42-43 1.4841607841200144 1103 44-45 1.3697200681161803 1103 46-47 1.4420526544101406 1103 48-49 1.2240040982964189 1103 50-51 0.9625574049104202 1103 52-53 0.527090111311523 1103 54-55 0.6572994442502136 1103 56-57 0.8131228210542645 1103 58-59 0.9656425684432648 1103 60-61 1.040754113526532 1103 62-63 0.9225621158995096 1103 64-65 0.7316156216275864 1103 66-67 0.6488651027797303 1103 68-69 0.5730753541633469 1103 70-71 0.8967737622982952 1103 72-73 0.40896817325738155 1103 74-75 0.2806705701446752 1103 76-77 0.4637845584755347 1103 78-79 0.38087189900765495 1103 80-81 0.26700887629254666 1103 82-83 0.37108675335620944 1103 84-85 0.35994277774049976 1103 86-87 0.5510881738868889 1103 88-89 0.47202830618132907 1103 90-91 0.5677791335251072 1103 92-93 0.1709640910571082 1103 94-95 0.18115155455618748 1103 96-97 -0.0614607762782029 1103 98-99 -0.1422228597982631 1103 100-101 -0.3772317663835807 1104 1 0.4726839931230913 1104 2 0.3140463585202866 1104 3 0.21926016053124187 1104 4 0.7037250131883113 1104 5 0.4648572739659187 1104 6 0.6933091133935179 1104 7 0.7157162336572327 1104 8 0.4704984978809321 1104 9 0.8703751886186026 1104 10-11 0.6027368866285485 1104 12-13 0.7542207135017591 1104 14-15 1.2555304269215242 1104 16-17 0.8979523992802996 1104 18-19 0.8087070853515144 1104 20-21 0.6949513734763286 1104 22-23 0.3872310986012124 1104 24-25 0.35872474200082394 1104 26-27 0.13771944010292714 1104 28-29 0.6322756284431605 1104 30-31 1.0521784084767987 1104 32-33 0.9990810037496871 1104 34-35 0.7837197389753641 1104 36-37 0.9274783103925017 1104 38-39 0.5841566632889013 1104 40-41 0.8636301284738366 1104 42-43 1.066123883292093 1104 44-45 1.02210948333488 1104 46-47 0.9872270761135766 1104 48-49 0.8571727592423031 1104 50-51 0.7369231996906436 1104 52-53 0.33934776641225994 1104 54-55 0.6082000345390952 1104 56-57 0.7328714041832782 1104 58-59 0.6292056658387111 1104 60-61 0.8965161480466648 1104 62-63 0.09784604529581031 1104 64-65 -0.9516712414467143 1104 66-67 -0.2969570710290306 1104 68-69 0.26175487704647793 1104 70-71 0.7949411573876617 1104 72-73 0.2060208373301542 1104 74-75 0.06712529467652928 1104 76-77 -0.14163769350541955 1104 78-79 -0.12461431203949402 1104 80-81 -0.04470384978498387 1104 82-83 -0.26272816065437965 1104 84-85 -0.4377158873804916 1104 86-87 -0.03189066410124397 1104 88-89 0.10551842805888256 1104 90-91 0.19859069190552248 1104 92-93 0.2116286213133023 1104 94-95 0.25007411078686914 1104 96-97 0.2557639776570575 1104 98-99 0.15158743494643545 1104 100-101 -0.13859643743855088 1105 1 -0.289714757409012 1105 2 -1.7561457781457577 1105 3 -0.405037113702182 1105 4 0.4112654380313785 1105 5 0.6297714979548203 1105 6 0.22705044236210625 1105 7 0.0727396441929713 1105 8 0.0945601978527506 1105 9 0.4792419739967144 1105 10-11 0.8992074695736676 1105 12-13 1.0744412040158338 1105 14-15 1.3504257600030414 1105 16-17 0.802130757225278 1105 18-19 0.7333260695892818 1105 20-21 1.0321879200126034 1105 22-23 0.3033701588622577 1105 24-25 -0.035912358722036686 1105 26-27 0.8771934175157838 1105 28-29 0.49798281088505547 1105 30-31 0.9488356271916416 1105 32-33 0.9981569430989268 1105 34-35 0.6179350393612779 1105 36-37 0.7775796348044892 1105 38-39 0.8056203263962232 1105 40-41 1.0001094399075043 1105 42-43 1.5100618305834175 1105 44-45 1.2879014525258583 1105 46-47 1.3160116778007236 1105 48-49 1.1128391571094909 1105 50-51 0.8695415164263594 1105 52-53 0.774875237918522 1105 54-55 0.06827057191820529 1105 56-57 0.277192465671412 1105 58-59 -0.36410095242406726 1105 60-61 0.22479999829437958 1105 62-63 0.8329592758258286 1105 64-65 1.0802759259663937 1105 66-67 0.9487253998476959 1105 68-69 0.9415638645418127 1105 70-71 0.2347594279578189 1105 72-73 -0.28823007648813714 1105 74-75 -0.26530546197900406 1105 76-77 -0.045583599859281065 1105 78-79 -0.4927549291391422 1105 80-81 -0.4154757539444809 1105 82-83 -0.39033983734357136 1105 84-85 -0.36509393835446247 1105 86-87 -0.49873215035249885 1105 88-89 -0.6611901073736846 1105 90-91 -0.6834861537676069 1105 92-93 -0.42386517531441825 1105 94-95 -0.7424251097945778 1105 96-97 -0.8639564608957819 1105 98-99 -1.497627001084286 1105 100-101 -0.8879029547766173 1106 1 0.2660959840529955 1106 2 0.3839987961236666 1106 3 -0.2456081904944014 1106 4 0.40872259351859697 1106 5 0.21073413538544372 1106 6 0.5577741555819316 1106 7 0.4961991074671772 1106 8 0.44803429341151 1106 9 0.9282428730130192 1106 10-11 0.25776966215468633 1106 12-13 0.442918786075964 1106 14-15 0.19043730171644313 1106 16-17 -0.21236769827638113 1106 18-19 0.44818615062577294 1106 20-21 0.38814118709454704 1106 22-23 0.40662649279657614 1106 24-25 0.2804705764721973 1106 26-27 0.721492675195222 1106 28-29 0.7970948528412478 1106 30-31 0.345652227772689 1106 32-33 0.419047907433999 1106 34-35 -1.105713766545584 1106 36-37 -1.0515452612233034 1106 38-39 -0.8457764459805688 1106 40-41 0.5805831150856022 1106 42-43 0.9494875159617919 1106 44-45 0.7016479832740856 1106 46-47 0.07721347005414003 1106 48-49 0.15589927821135063 1106 50-51 0.7898631747729397 1106 52-53 0.21993878448829207 1106 54-55 0.8253834931636561 1106 56-57 0.35374578330275597 1106 58-59 0.8283532287241613 1106 60-61 0.9861393242053396 1106 62-63 0.8221373218976638 1106 64-65 0.8415203212383098 1106 66-67 0.714526049025082 1106 68-69 0.6694731575289694 1106 70-71 1.071264880171725 1106 72-73 0.4067219381576699 1106 74-75 0.43422730330791026 1106 76-77 0.48788834146858306 1106 78-79 0.5614330077791259 1106 80-81 0.5760735668754187 1106 82-83 0.46386226044758416 1106 84-85 0.6905940878912205 1106 86-87 0.8115865693119524 1106 88-89 0.8498160119942284 1106 90-91 1.0067835576071325 1106 92-93 0.5571986628081902 1106 94-95 0.7278185340897565 1106 96-97 0.8858743078747615 1106 98-99 0.8202725552608463 1106 100-101 0.5873144062868363 1107 1 0.5005331665561172 1107 2 0.5359846207500389 1107 3 0.20692169320124165 1107 4 -0.8832811398190685 1107 5 0.39519701992719547 1107 6 0.6054097033812553 1107 7 0.7265821655976978 1107 8 0.5745081752374759 1107 9 0.9157341465016557 1107 10-11 0.7436305330642767 1107 12-13 1.0614139068996096 1107 14-15 1.424091918052227 1107 16-17 1.017769318103447 1107 18-19 0.880676339786163 1107 20-21 0.4382316630830587 1107 22-23 0.6810956156096069 1107 24-25 0.5794801080333656 1107 26-27 0.9123310639646505 1107 28-29 0.8221556188371935 1107 30-31 1.2973052493488666 1107 32-33 1.2771127228921628 1107 34-35 1.0081385945679173 1107 36-37 1.21670860032377 1107 38-39 1.0270275637179225 1107 40-41 0.3654632930538 1107 42-43 0.09875778259826262 1107 44-45 -0.10330753219692212 1107 46-47 0.28150414642531985 1107 48-49 0.6280750542880895 1107 50-51 -1.0244911806924222 1107 52-53 0.48334347293717883 1107 54-55 0.9478932603895203 1107 56-57 0.9075891479507661 1107 58-59 0.79669054294083 1107 60-61 1.1068582954981636 1107 62-63 1.196287267199999 1107 64-65 1.1229193693755697 1107 66-67 1.0187326110006794 1107 68-69 1.0886849125495885 1107 70-71 1.4830139452581932 1107 72-73 0.9051323670794247 1107 74-75 0.7330771934764329 1107 76-77 0.8337592014326276 1107 78-79 0.9480575405479463 1107 80-81 0.8891091325413463 1107 82-83 1.087071201328083 1107 84-85 0.9523928125046268 1107 86-87 1.2136608997881346 1107 88-89 1.176748575198836 1107 90-91 1.2375268804160164 1107 92-93 1.1306363352893243 1107 94-95 1.0578985066822675 1107 96-97 0.9619716676181795 1107 98-99 1.0355248728808952 1107 100-101 0.9795357873149726 1108 1 0.5759771669695439 1108 2 0.5761352380301048 1108 3 0.41373897714797536 1108 4 0.6730190727177146 1108 5 0.7360216059893787 1108 6 0.8526449151284368 1108 7 0.8049880924936517 1108 8 0.7808556327445473 1108 9 1.0905825087722079 1108 10-11 0.9630751567650435 1108 12-13 1.1123860095447498 1108 14-15 1.423483452152297 1108 16-17 1.0621001052975174 1108 18-19 0.8721049584334679 1108 20-21 0.8778933303347145 1108 22-23 0.8716668993769048 1108 24-25 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84-85 1.7467984916103418 2311 86-87 1.8228841290557334 2311 88-89 1.9549821165060806 2311 90-91 2.0544537114135792 2311 92-93 2.1016007355130526 2311 94-95 2.145676396062207 2311 96-97 2.0672387644127 2311 98-99 2.252069335181684 2311 100-101 2.381326706575404 2312 1 -0.14730702215094382 2312 2 -0.2874323776282601 2312 3 -0.3087572099389604 2312 4 0.0718180051020596 2312 5 -0.9203141845545986 2312 6 -0.012968963875124473 2312 7 0.29146496082105244 2312 8 -2.9461906306006505 2312 9 -6.139317084517064 2312 10-11 -0.8056526012420733 2312 12-13 -5.65199185014005 2312 14-15 -2.3949805793677896 2312 16-17 -2.842529406649618 2312 18-19 -2.292079078170488 2312 20-21 -4.1180109655473345 2312 22-23 -2.396978245513793 2312 24-25 -2.4194667075349656 2312 26-27 -3.1810006108105213 2312 28-29 -6.29701270638763 2312 30-31 -6.769175290235388 2312 32-33 -1.4367961301227297 2312 34-35 0.2784992388256171 2312 36-37 0.7879455161427273 2312 38-39 0.6180215929085477 2312 40-41 -2.7343164872793935 2312 42-43 -4.641202412005896 2312 44-45 -3.5834011485908484 2312 46-47 0.28115677063289723 2312 48-49 -1.4609913617520078 2312 50-51 -6.790624344484254 2312 52-53 -1.6219224492211595 2312 54-55 0.7964300435238201 2312 56-57 1.4089233658484517 2312 58-59 1.5142049171115488 2312 60-61 1.834023635318296 2312 62-63 1.8910775517348668 2312 64-65 1.7121203129108729 2312 66-67 1.3710011499952302 2312 68-69 1.8400661229284836 2312 70-71 2.069443253823721 2312 72-73 1.477256611514047 2312 74-75 1.5138084333408024 2312 76-77 1.6181088623496258 2312 78-79 1.3521302222522102 2312 80-81 1.1921148084959405 2312 82-83 0.41585824246032743 2312 84-85 0.9588418394838385 2312 86-87 1.5732998869619514 2312 88-89 1.8784145047285463 2312 90-91 1.9454160887309193 2312 92-93 1.944110277497785 2312 94-95 2.0376134189629695 2312 96-97 2.2410664634312383 2312 98-99 1.9863864017029478 2312 100-101 2.0698286149723515 2313 1 -0.36984015911707147 2313 2 -0.4459927605443106 2313 3 -0.1857086090553075 2313 4 -1.0149824367241607 2313 5 -1.1607965115059926 2313 6 -0.861625075804433 2313 7 -1.9873273808578844 2313 8 -3.5460433551956427 2313 9 -1.569195582307934 2313 10-11 -0.7077328663378069 2313 12-13 -4.357026828048745 2313 14-15 -2.967338826790467 2313 16-17 -6.598558493542107 2313 18-19 -4.0503265008509075 2313 20-21 -4.5196401997152265 2313 22-23 -2.958502545955618 2313 24-25 -1.1363205366955 2313 26-27 -3.7748610673642773 2313 28-29 -6.2045697756070695 2313 30-31 -3.975084715861307 2313 32-33 -7.189898118340697 2313 34-35 -3.290729406240658 2313 36-37 -5.216251832899985 2313 38-39 -2.836709629477312 2313 40-41 -1.5824663400039825 2313 42-43 -2.587152338368192 2313 44-45 -4.164881266411172 2313 46-47 -1.7427662779679878 2313 48-49 -0.6295664722085661 2313 50-51 0.9155336083876122 2313 52-53 0.2298724697773693 2313 54-55 -5.047273932912113 2313 56-57 -4.245568534004274 2313 58-59 0.4601548434738447 2313 60-61 -8.528955009747975 2313 62-63 -2.0539334939205105 2313 64-65 -2.3216057548358116 2313 66-67 -2.635534196101972 2313 68-69 -5.0397847607239505 2313 70-71 -4.536226849269063 2313 72-73 -0.4891922102827202 2313 74-75 0.8472399502774728 2313 76-77 -1.9423384866930853 2313 78-79 0.2715337568619276 2313 80-81 0.9914704785990303 2313 82-83 1.9075924103542867 2313 84-85 1.5743885994249283 2313 86-87 1.7051389885819788 2313 88-89 -2.949199633710336 2313 90-91 1.1531296380681795 2313 92-93 2.1455922362606756 2313 94-95 2.054688161231013 2313 96-97 2.066471470795012 2313 98-99 2.1676916299798243 2313 100-101 2.1312332541476096 2314 1 -28.761592736436658 2314 2 -14.779372532068415 2314 3 -5.833660684842435 2314 4 -3.592998597214546 2314 5 -1.2969068486472608 2314 6 -1.2831909715971292 2314 7 -0.5900022206461273 2314 8 -4.711827696237716 2314 9 -5.641778474478457 2314 10-11 -1.6396776977671692 2314 12-13 -0.09636926326746931 2314 14-15 -1.7299467955839987 2314 16-17 -1.0972350051052189 2314 18-19 -3.7123493484407675 2314 20-21 -3.2177455215318957 2314 22-23 -5.398040021575568 2314 24-25 -1.334983116801375 2314 26-27 -3.603225514285427 2314 28-29 -1.7974229380478661 2314 30-31 -3.4142042477643457 2314 32-33 -2.7842140837906797 2314 34-35 -2.6911194870431103 2314 36-37 -8.761668383471175 2314 38-39 -8.939941727554771 2314 40-41 -8.227632124345025 2314 42-43 -5.315140635944118 2314 44-45 -5.2014513416410395 2314 46-47 -7.281077785351659 2314 48-49 -6.730151593412241 2314 50-51 -3.7139592865691995 2314 52-53 -5.491637700186413 2314 54-55 -6.127797755703977 2314 56-57 -2.8242812673561843 2314 58-59 -2.1159109130042815 2314 60-61 -0.7604261716315079 2314 62-63 -0.3016348034774907 2314 64-65 -2.2353700345794714 2314 66-67 -1.2214216299275513 2314 68-69 -0.20933059521823694 2314 70-71 0.7856594700399384 2314 72-73 0.39677881228623946 2314 74-75 0.7547350742674404 2314 76-77 -1.5755928751021138 2314 78-79 -3.8881207429987583 2314 80-81 -1.3676824888013606 2314 82-83 -2.000599286497202 2314 84-85 -0.9076398207478178 2314 86-87 0.5149745973866615 2314 88-89 0.4664453927594323 2314 90-91 0.6289585597733911 2314 92-93 0.9824548720923829 2314 94-95 1.1938634189629695 2314 96-97 1.4956996680644465 2314 98-99 0.9602522318187816 2314 100-101 0.8558083447020799 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 21.0 4 51.0 5 101.0 6 215.0 7 684.0 8 1508.0 9 2530.0 10 3977.0 11 5984.0 12 7868.0 13 10085.0 14 12467.0 15 15653.0 16 18570.0 17 21625.0 18 19003.0 19 14894.0 20 13760.0 21 13973.0 22 15377.0 23 17321.0 24 20414.0 25 25246.0 26 31995.0 27 40095.0 28 50399.0 29 61873.0 30 75298.0 31 91754.0 32 110807.0 33 133884.0 34 165582.0 35 201754.0 36 250480.0 37 296838.0 38 243292.0 39 50987.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.95010548399344 22.637324894613858 15.134066437302584 22.278503184090116 2 14.999384279783268 22.053338962775314 40.35831007279182 22.588966684649606 3 17.53162534171398 28.187432744531353 31.235809768059653 23.045132145695018 4 12.245385629934631 18.555653669237223 39.903947411500994 29.295013289327148 5 11.592829669215398 39.584055137445354 35.88400377838492 12.939111414954333 6 28.164948514061876 40.3260580614391 18.140094918646746 13.368898505852275 7 25.55624946246648 34.03672038092557 23.53048108272934 16.876549073878607 8 25.597786143754053 35.56810451997279 20.48843227859483 18.345677057678326 9 26.116603726377846 15.660794845933118 21.9342997208735 36.28830170681553 10-11 23.79262640833783 27.39566474327399 28.83493518323052 19.976773665157662 12-13 24.692555258446117 25.469388931891572 28.77939819701483 21.05865761264748 14-15 22.401856152120033 26.524005269783192 26.871911625579557 24.20222695251722 16-17 20.707452755690035 29.365040969827756 27.84267273395414 22.084833540528074 18-19 20.522932157405453 28.33669047451505 30.546197585594882 20.594179782484616 20-21 21.669759927755493 27.465837301308067 30.30648206788169 20.557920703054755 22-23 21.82034261409393 27.22993338493655 29.966956348368633 20.98276765260088 24-25 21.33276084492056 27.663457945683817 30.315558578198797 20.688222631196822 26-27 21.139421822265618 27.62273594947176 30.18281985043656 21.055022377826063 28-29 20.84735164736621 27.44993785068183 30.58968182041425 21.113028681537706 30-31 21.46114872294074 27.28915792179411 30.24799862388594 21.00169473137921 32-33 21.471932297496366 27.719499629709176 29.851255726924503 20.957312345869955 34-35 21.586029558007944 27.520574447467173 30.274112578059754 20.61928341646513 36-37 21.565169373912703 27.12765593541703 30.104136124631054 21.20303856603921 38-39 21.380453158187553 27.601342180537603 30.23300021525906 20.78520444601579 40-41 21.134140268762373 27.12275019388245 30.70203773267727 21.041071804677905 42-43 21.237715074013146 27.258504676231134 30.063730248766163 21.440050000989565 44-45 21.33685501644375 27.63019395319517 29.85728387339533 21.17566715696575 46-47 21.480837160899025 27.436662168823815 29.534965738266607 21.547534932010556 48-49 21.680075873227587 27.504509310525748 29.342274813670965 21.473140002575704 50-51 21.64824412591221 27.503122322915317 28.802404830594874 22.04622872057759 52-53 21.33378291209758 28.060409703082545 28.76891162845247 21.836895756367404 54-55 21.608939563449297 27.93393589085817 28.673318313800362 21.783806231892168 56-57 21.85385431464218 27.83051346067107 28.45088055966446 21.864751665022293 58-59 21.299437609661474 27.810699465806145 29.058308323411218 21.831554601121166 60-61 21.64795627277714 28.338323993407688 28.445491851846423 21.568227881968753 62-63 21.637077822025162 27.838835804513757 28.626297021535702 21.897789351925383 64-65 21.298829697575556 27.762409034784564 28.69258112859545 22.24618013904443 66-67 21.638809102893187 27.713610968236146 28.31157629299128 22.336003635879386 68-69 21.452141424443145 27.745607821587505 28.47175916570906 22.330491588260287 70-71 21.66591244956682 27.431275021111563 28.684187634879432 22.218624894442186 72-73 21.863343959915625 27.55776508973463 28.57866468649139 22.000226263858355 74-75 21.685084324096366 28.062235368597076 28.266598187367283 21.986082119939276 76-77 21.508866371120963 27.993059953557104 28.25293297836574 22.24514069695619 78-79 21.347239814228413 28.015929561607205 28.115568730013525 22.521261894150854 80-81 21.281187152602246 28.19619370931679 28.121964908199885 22.400654229881077 82-83 21.813670514934948 27.766856025899457 28.45561053081669 21.96386292834891 84-85 21.749246903134235 27.982755920383447 27.80935245031051 22.458644726171805 86-87 21.169476219145096 28.36313107422379 28.216968537182264 22.250424169448856 88-89 21.799796559992203 27.658507156929556 28.018448876229744 22.523247406848494 90-91 21.587796853677816 27.605196061782948 28.427584134248292 22.379422950290945 92-93 21.677674258361982 27.998670781380184 28.00700283191982 22.316652128338013 94-95 21.15690363534409 27.605623134652014 28.26198129123923 22.975491938764662 96-97 21.5169559628504 27.612883174609536 27.939631773167616 22.93052908937245 98-99 21.05357163800417 27.995122032268277 27.954390855105494 22.996915474622057 100-101 21.22587097352731 27.86179459531309 27.776812628935943 23.135521802223654 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 780.0 1 511.5 2 274.5 3 323.5 4 304.0 5 307.0 6 562.0 7 1110.5 8 1759.0 9 2171.5 10 2002.5 11 1665.5 12 1709.5 13 1918.5 14 2156.0 15 2395.5 16 2658.0 17 2906.0 18 3102.5 19 3326.5 20 3681.0 21 4101.5 22 4605.5 23 5459.5 24 6350.0 25 7725.0 26 10797.5 27 14125.0 28 16362.0 29 20633.0 30 27546.5 31 33807.5 32 41797.0 33 51261.0 34 60365.0 35 68208.0 36 76374.0 37 84977.5 38 85026.5 39 79816.5 40 74042.0 41 67668.5 42 63249.0 43 63285.0 44 65932.5 45 69302.0 46 69604.0 47 68045.0 48 67825.0 49 66286.0 50 64762.5 51 62752.5 52 60506.0 53 59091.5 54 57318.5 55 52797.5 56 47744.5 57 41611.0 58 36099.5 59 33140.5 60 28501.0 61 22842.5 62 17900.0 63 13507.0 64 9950.0 65 7047.5 66 5011.5 67 3767.5 68 2921.5 69 2259.0 70 1682.0 71 1248.5 72 969.0 73 752.0 74 566.0 75 440.5 76 327.0 77 230.5 78 170.0 79 143.5 80 112.0 81 88.5 82 71.5 83 56.5 84 42.0 85 39.0 86 35.5 87 20.0 88 14.0 89 13.5 90 8.5 91 3.5 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12079844252105176 2 0.0 3 0.00400706807715463 4 2.443334193386969E-4 5 3.420667870741757E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.01688343927630396 26-27 0.007916402786573781 28-29 0.005008835096443288 30-31 0.002052400722445054 32-33 9.529003354209182E-4 34-35 0.0017103339353708785 36-37 0.0011728004128257455 38-39 6.108335483467423E-4 40-41 0.002003534038577315 42-43 0.001197233754759615 44-45 6.841335741483514E-4 46-47 0.028758043456164627 48-49 0.016590239173097522 50-51 0.08954819818763243 52-53 0.0011728004128257455 54-55 0.040339447532818866 56-57 7.330002580160908E-4 58-59 0.001441567174098312 60-61 0.0030053010578659726 62-63 0.0029808677159321026 64-65 0.009944370167084965 66-67 0.005448635251252942 68-69 0.002321167483717621 70-71 0.0054730685931868115 72-73 0.004911101728707809 74-75 0.0011483670708918757 76-77 0.0 78-79 0.0 80-81 4.153668128757848E-4 82-83 4.398001548096545E-4 84-85 2.4433341933869695E-5 86-87 0.0015637338837676605 88-89 0.0039337680513530215 90-91 0.0012949671224950937 92-93 0.0033718011868740182 94-95 8.063002838176998E-4 96-97 4.642334967435242E-4 98-99 0.0019546673547095753 100-101 0.0032985011610724086 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2046384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.77482461315022 #Duplication Level Percentage of deduplicated Percentage of total 1 74.99275195342744 30.57816308158483 2 13.22919084904037 10.788358732870257 3 4.6129982419045525 5.642825827632854 4 2.112097998641028 3.4448170184149403 5 1.1353644452911087 2.3147143064375775 6 0.679088931918517 1.6613839257425427 7 0.4721332913139832 1.3475806503149919 8 0.32782676598689436 1.0693663109289488 9 0.258424119694017 0.9483478340698154 >10 1.7212911223594793 14.09739294877711 >50 0.2494914364030109 7.178397372857742 >100 0.1943709305633276 14.852413997800722 >500 0.011902308239756718 3.2046769420149714 >1k 0.002699492590460286 1.7201648339383118 >5k 3.681126259718572E-4 1.151396216614478 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9761 0.4769877012330042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7976 0.38976067052908936 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5276 0.257820624086193 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 3000 0.14660005160321818 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 2338 0.11425030688277468 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 2338 0.11425030688277468 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 2168 0.10594297062525901 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.886668386773939E-5 0.0 0.0 4.886668386773939E-5 0.0 12-13 4.886668386773939E-5 0.0 0.0 4.886668386773939E-5 0.0 14-15 4.886668386773939E-5 0.0 0.0 4.886668386773939E-5 0.0 16-17 4.886668386773939E-5 0.0 0.0 4.886668386773939E-5 0.0 18-19 4.886668386773939E-5 0.0 0.0 4.886668386773939E-5 0.0 20-21 4.886668386773939E-5 0.0 0.0 9.773336773547878E-5 0.0 22-23 4.886668386773939E-5 0.0 0.0 1.7103339353708786E-4 0.0 24-25 4.886668386773939E-5 0.0 0.0 6.841335741483514E-4 0.0 26-27 4.886668386773939E-5 0.0 0.0 0.002150134090180533 0.0 28-29 4.886668386773939E-5 0.0 0.0 0.004691201651302981 0.0 30-31 9.773336773547878E-5 0.0 0.0 0.012534304412075151 0.0 32-33 9.773336773547878E-5 0.0 0.0 0.03054167741733712 0.0 34-35 9.773336773547878E-5 0.0 0.0 0.05822465382841148 0.0 36-37 9.773336773547878E-5 0.0 0.0 0.10235126936098016 0.0 38-39 9.773336773547878E-5 0.0 0.0 0.16416762445367047 0.0 40-41 9.773336773547878E-5 0.0 0.0 0.2295268141267719 0.0 42-43 9.773336773547878E-5 0.0 0.0 0.2933711365999734 0.0 44-45 9.773336773547878E-5 0.0 0.0 0.3690900632530356 0.0 46-47 9.773336773547878E-5 0.0 0.0 0.46501536368540797 0.0 48-49 9.773336773547878E-5 0.0 0.0 0.5649965988788028 0.0 50-51 9.773336773547878E-5 0.0 0.0 0.6680320018139313 0.0 52-53 9.773336773547878E-5 0.0 0.0 0.7706031712523163 0.0 54-55 9.773336773547878E-5 0.0 0.0 0.8800156764321848 0.0 56-57 9.773336773547878E-5 0.0 0.0 1.0107584891203216 0.0 58-59 9.773336773547878E-5 0.0 0.0 1.153546939381856 0.0 60-61 9.773336773547878E-5 0.0 0.0 1.3011487580043628 0.0 62-63 9.773336773547878E-5 0.0 0.0 1.446453842485086 0.0 64-65 9.773336773547878E-5 0.0 0.0 1.6023141306812407 0.0 66-67 9.773336773547878E-5 0.0 0.0 1.7789916262050522 0.0 68-69 9.773336773547878E-5 0.0 0.0 1.9703535602311197 0.0 70-71 9.773336773547878E-5 0.0 0.0 2.1826793016364476 0.0 72-73 9.773336773547878E-5 0.0 0.0 2.402970312512217 0.0 74-75 9.773336773547878E-5 0.0 0.0 2.6227970898912423 0.0 76-77 9.773336773547878E-5 0.0 0.0 2.8672282425976747 0.0 78-79 9.773336773547878E-5 0.0 0.0 3.1292269681545593 0.0 80-81 9.773336773547878E-5 0.0 0.0 3.4125560012197127 0.0 82-83 9.773336773547878E-5 0.0 0.0 3.7078573718324614 0.0 84-85 9.773336773547878E-5 0.0 0.0 4.002792242316202 0.0 86-87 9.773336773547878E-5 0.0 0.0 4.3108966841023 0.0 88-89 9.773336773547878E-5 0.0 0.0 4.641675267203027 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3705 0.0 57.61983 1 GTATCAA 7655 0.0 45.837933 1 ATCAACG 9950 0.0 34.55316 3 CAACGCA 10050 0.0 34.161266 5 TCAACGC 10545 0.0 32.8737 4 TATCAAC 10630 0.0 32.655502 2 AACGCAG 10880 0.0 32.122593 6 GTGGTAT 1715 0.0 28.83255 1 CGCAGAG 12090 0.0 28.082865 8 ACGCAGA 12270 0.0 27.941795 7 GTGATCG 585 0.0 25.975014 8 GTACATG 9420 0.0 25.842402 1 GCAGAGT 13060 0.0 25.451683 9 TAGTACT 1185 0.0 24.845379 4 TGGTATC 1915 0.0 24.797249 2 ACATGGG 9575 0.0 24.301304 3 TACATGG 9900 0.0 24.223032 2 GTACTAG 1040 0.0 23.772991 1 GCTATTG 1495 0.0 23.186935 8 TGATCGC 640 0.0 23.000824 9 >>END_MODULE