FastQCFastQC Report
Sat 18 Jun 2016
SRR1931118_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931118_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388290
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46440.19444874784887933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35860.15014926997977632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.048.696261
GTATCAA51900.042.8049851
ATCAACG59050.035.594633
TCAACGC60800.035.123284
CAACGCA60650.034.5792275
TATCAAC64900.033.4946482
AACGCAG63200.033.4639366
ACGCAGA72350.028.452487
CGCAGAG72900.027.9763748
CAACGCG1701.1110933E-725.1768325
GCAGAGT80800.023.475959
GAGTACT52050.021.27810912-13
GTGGTAT16200.020.9388121
GTACATG51300.020.4886821
CAGAGTA79850.020.44923410-11
AGAGTAC73600.019.92519410-11
GTACTTT59900.019.11759214-15
TACATGG51800.019.0919062
GTATAGG7800.018.987851
TGGTATC16700.018.5954042
Produced by FastQC (version 0.11.5)