FastQCFastQC Report
Sat 18 Jun 2016
SRR1931118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388290
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61330.2567946103697624No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48490.2030322950730439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32870.13762985232111677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29550.053.2480281
GTATCAA54650.042.187581
ATCAACG67950.033.1992153
TCAACGC69150.032.6897164
CAACGCA69250.032.5739335
AACGCAG72150.031.3304776
TATCAAC73750.030.8458772
ACGCAGA84100.026.4268997
CGCAGAG83700.026.2127678
GTGGTAT15250.024.6257971
GCAGAGT94150.022.445859
TGGTATC15900.022.1033572
GTACATG59350.021.065321
AGAGTAC85050.020.91095510-11
GAGTACT61850.019.96319412-13
TACATGG59850.019.7587222
ACATGGG57150.019.3625833
CAGAGTA92850.019.28216610-11
GGACCGT3506.366463E-1118.9957456
AGTACTT67350.018.29768412-13
Produced by FastQC (version 0.11.5)