##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931118_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2388290 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.275180149814304 31.0 31.0 34.0 30.0 34.0 2 31.265491209191513 31.0 31.0 34.0 28.0 34.0 3 31.42965720243354 31.0 31.0 34.0 28.0 34.0 4 35.10495333481278 35.0 35.0 37.0 32.0 37.0 5 34.940997533800335 35.0 35.0 37.0 32.0 37.0 6 35.06951835832332 36.0 35.0 37.0 32.0 37.0 7 34.895416804491916 36.0 35.0 37.0 32.0 37.0 8 34.96710868445624 36.0 35.0 37.0 32.0 37.0 9 36.35942368807808 38.0 35.0 39.0 32.0 39.0 10-11 36.23244811141026 38.0 35.0 39.0 31.5 39.0 12-13 36.176769152824825 38.0 35.0 39.0 32.0 39.0 14-15 37.14635178307492 39.0 36.0 40.0 31.5 41.0 16-17 37.18466601627105 39.0 36.0 40.0 31.5 41.0 18-19 37.2360550854377 39.0 36.0 40.0 31.5 41.0 20-21 37.20703725259496 39.0 36.0 40.0 31.0 41.0 22-23 37.095377655142386 39.0 36.0 40.0 31.0 41.0 24-25 36.968398519442786 39.0 36.0 40.0 31.0 41.0 26-27 36.7216996260923 39.0 35.5 40.0 30.0 41.0 28-29 36.44141896503356 38.0 35.0 40.0 30.0 41.0 30-31 36.213371701091575 38.0 35.0 40.0 30.0 41.0 32-33 36.07863680708792 38.0 35.0 40.0 29.5 41.0 34-35 35.85672280166982 38.0 34.0 40.0 29.0 41.0 36-37 36.09340197379716 38.0 35.0 40.0 30.0 41.0 38-39 36.071782321242395 38.0 35.0 40.0 30.0 41.0 40-41 35.919533641224476 38.0 35.0 40.0 29.0 41.0 42-43 35.80431626812489 38.0 35.0 40.0 29.0 41.0 44-45 35.56541458533093 38.0 34.0 40.0 27.5 41.0 46-47 35.380080936569684 38.0 34.0 40.0 27.0 41.0 48-49 35.15397292623592 38.0 34.0 40.0 27.0 41.0 50-51 34.81941284349891 38.0 33.0 40.0 26.0 41.0 52-53 34.689730937197744 38.0 33.0 40.0 25.5 41.0 54-55 34.452906891541645 38.0 33.0 40.0 24.5 41.0 56-57 34.25281791574725 38.0 33.0 40.0 24.0 41.0 58-59 34.02803721491109 37.0 33.0 40.0 24.0 41.0 60-61 33.7550996738252 37.0 32.0 40.0 23.0 41.0 62-63 33.49632645114286 36.5 32.0 40.0 22.5 41.0 64-65 33.25853895464956 36.0 32.0 39.0 22.0 41.0 66-67 33.01548095080581 36.0 31.0 39.0 22.0 40.5 68-69 32.71530802373246 35.5 31.0 39.0 22.0 40.0 70-71 31.873995201587746 35.0 30.0 38.5 18.5 40.0 72-73 31.83917740307919 35.0 31.0 38.0 20.0 40.0 74-75 31.530489178449855 35.0 30.0 37.0 19.0 39.5 76-77 30.79357678506379 34.0 29.5 36.0 18.0 39.0 78-79 30.81402363197099 34.0 30.0 36.0 18.0 39.0 80-81 30.529790770802542 34.0 30.0 36.0 16.0 38.5 82-83 30.165800635601205 34.0 29.5 35.5 12.5 37.0 84-85 29.80044215735945 34.0 29.0 35.0 9.0 37.0 86-87 29.448446168597616 34.0 29.0 35.0 7.0 36.5 88-89 29.121310644854688 34.0 29.0 35.0 3.5 36.0 90-91 28.808678594308063 34.0 29.0 35.0 2.0 36.0 92-93 28.45577044663755 34.0 29.0 35.0 2.0 35.5 94-95 28.059206796494564 33.0 28.5 35.0 2.0 35.0 96-97 27.67645721415741 33.0 27.0 35.0 2.0 35.0 98-99 27.19755033936415 33.0 26.5 35.0 2.0 35.0 100-101 26.50960519869865 32.5 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8139765986149072 1101 2 0.7744614538518952 1101 3 0.5344654062363254 1101 4 0.844743892809106 1101 5 0.7841865706369546 1101 6 0.7892824991483067 1101 7 0.7653362477530194 1101 8 0.8197019087343733 1101 9 0.9838292186783093 1101 10-11 1.0083413253165716 1101 12-13 1.1014120220172998 1101 14-15 1.3899939376277999 1101 16-17 1.0503151878659267 1101 18-19 0.9889980202263189 1101 20-21 0.9996873163391129 1101 22-23 0.845699396086971 1101 24-25 0.6745385690871117 1101 26-27 0.9850148424288676 1101 28-29 0.9023053720051593 1101 30-31 1.1956557967391817 1101 32-33 1.1507745710057549 1101 34-35 0.861242443456689 1101 36-37 1.1081677688977791 1101 38-39 0.8012712572342906 1101 40-41 0.8573344324744312 1101 42-43 0.9721226270510357 1101 44-45 0.9738569878303167 1101 46-47 1.0791222501657742 1101 48-49 1.0628914098907316 1101 50-51 1.031559169694738 1101 52-53 0.8856844307915779 1101 54-55 0.7825653351102559 1101 56-57 0.4975434771000735 1101 58-59 0.8632371843522648 1101 60-61 1.0220070954024436 1101 62-63 0.9194728567038766 1101 64-65 0.719146997325069 1101 66-67 0.49753451561794293 1101 68-69 0.4612566655533996 1101 70-71 0.8420865776349977 1101 72-73 0.4051957138924678 1101 74-75 0.10601846223497446 1101 76-77 0.09615320350985357 1101 78-79 0.2538643710315185 1101 80-81 0.13068221368052235 1101 82-83 -0.0417986670769217 1101 84-85 0.17396868921409592 1101 86-87 0.041169887971822305 1101 88-89 -0.07229392698401327 1101 90-91 -0.05306221923102328 1101 92-93 -0.2530453956249197 1101 94-95 -0.2044882027308219 1101 96-97 -0.24086020201043112 1101 98-99 -0.17580595663203624 1101 100-101 -0.2982813390639869 1102 1 0.9335895401811598 1102 2 0.8593553969424441 1102 3 0.671811406085066 1102 4 0.9476966705789778 1102 5 0.835099298320479 1102 6 0.8337437782739698 1102 7 0.8788698758198521 1102 8 0.9811128005832614 1102 9 1.26521664323473 1102 10-11 1.2784451623897652 1102 12-13 1.2663066960874332 1102 14-15 1.649877113800521 1102 16-17 1.4641147374432748 1102 18-19 1.2798358873734799 1102 20-21 1.3453012648218277 1102 22-23 0.9146147109951954 1102 24-25 0.6733799980983903 1102 26-27 1.070609861388455 1102 28-29 1.264340571045203 1102 30-31 1.5458954061492634 1102 32-33 1.454926076174985 1102 34-35 1.4348202847126075 1102 36-37 1.644611451984268 1102 38-39 1.3883033947040104 1102 40-41 1.371060630940761 1102 42-43 1.5858635242192989 1102 44-45 1.5091826835345898 1102 46-47 1.500914396913565 1102 48-49 1.3164078646840736 1102 50-51 1.3319534081833169 1102 52-53 1.3484372239405005 1102 54-55 1.0627176889752548 1102 56-57 1.1709152200876574 1102 58-59 1.3902835869992387 1102 60-61 1.7515725770910962 1102 62-63 1.6735571655468675 1102 64-65 1.5548665359661982 1102 66-67 1.1766434198668918 1102 68-69 1.202363792667576 1102 70-71 1.5083383105470851 1102 72-73 0.91284362729386 1102 74-75 0.9991578707997135 1102 76-77 1.0069553412612144 1102 78-79 1.0785024491184885 1102 80-81 0.8611989181461084 1102 82-83 0.8654329835161043 1102 84-85 0.9577633624298336 1102 86-87 0.8991808229200196 1102 88-89 0.8177902400932346 1102 90-91 0.8988983407816065 1102 92-93 0.830954284136407 1102 94-95 0.7193114481077636 1102 96-97 0.8148857721432705 1102 98-99 0.9577688531154998 1102 100-101 0.8171315552259273 1103 1 0.6737282364363608 1103 2 0.48732765769140585 1103 3 0.3757537084429039 1103 4 0.6988471097837845 1103 5 0.6572932881494182 1103 6 0.6390778513206641 1103 7 0.5305259091069487 1103 8 0.6662667534265339 1103 9 0.7673610722684785 1103 10-11 0.8153899612801965 1103 12-13 0.9285726230407363 1103 14-15 1.232807118423736 1103 16-17 0.9676736833517339 1103 18-19 0.8951085642111991 1103 20-21 0.9263379730973682 1103 22-23 0.7539521127520104 1103 24-25 0.4681622449777265 1103 26-27 0.8020044060024176 1103 28-29 0.8053602659134427 1103 30-31 1.1024030344432916 1103 32-33 1.126119233826401 1103 34-35 0.9400770577130686 1103 36-37 1.2077376655765093 1103 38-39 0.8955810646069295 1103 40-41 0.8453265578940687 1103 42-43 1.172144060511485 1103 44-45 1.0824466687403245 1103 46-47 1.0841683959889252 1103 48-49 1.089889695474639 1103 50-51 0.9116876661583504 1103 52-53 0.4550576585221009 1103 54-55 0.6772881929049817 1103 56-57 0.699859464193068 1103 58-59 1.0198728611554984 1103 60-61 0.9663792345113436 1103 62-63 0.7860226301793176 1103 64-65 0.8703012100299929 1103 66-67 0.7282116121923679 1103 68-69 0.5291092388072016 1103 70-71 0.8318495449437613 1103 72-73 0.2674540757450785 1103 74-75 0.0912065069532062 1103 76-77 0.2890716611872435 1103 78-79 0.2679117879996724 1103 80-81 0.18091468328711358 1103 82-83 0.27132202651194604 1103 84-85 0.2638957711214651 1103 86-87 0.2697896070161825 1103 88-89 0.15389768345893273 1103 90-91 -0.0054152976834878075 1103 92-93 -0.2717619433254548 1103 94-95 -0.32299904657554634 1103 96-97 -0.3434596739963496 1103 98-99 -0.4136996628345706 1103 100-101 -0.3898193462997348 1104 1 0.5161668169641125 1104 2 0.42427761219549964 1104 3 0.18479319888876233 1104 4 0.618853297226913 1104 5 0.3120259542094743 1104 6 0.5357249850786232 1104 7 0.4912756816274282 1104 8 0.49217758145201174 1104 9 0.6655747210400946 1104 10-11 0.6512299976769214 1104 12-13 0.7315229415120754 1104 14-15 1.0449501575502111 1104 16-17 0.876086786172479 1104 18-19 0.7476696197162056 1104 20-21 0.7120748247807214 1104 22-23 0.3778771354999577 1104 24-25 0.34175460166562033 1104 26-27 0.20743916487411695 1104 28-29 0.721708036614082 1104 30-31 0.9327961190656779 1104 32-33 0.9962526278209367 1104 34-35 0.8160104517076121 1104 36-37 0.9313582297257383 1104 38-39 0.36145749772794744 1104 40-41 0.6061813349641341 1104 42-43 0.9046687192921965 1104 44-45 0.8775467597321338 1104 46-47 0.7934648473083001 1104 48-49 0.743153935693023 1104 50-51 0.6670428981874679 1104 52-53 0.5096436457832425 1104 54-55 0.7131453357621282 1104 56-57 0.8247664705624942 1104 58-59 0.7796482933665985 1104 60-61 1.0158320097615672 1104 62-63 0.18674656102554366 1104 64-65 -0.7129331848744584 1104 66-67 -0.09047901565112682 1104 68-69 0.31815455272894866 1104 70-71 1.0274178797936209 1104 72-73 0.6217578064093203 1104 74-75 0.3054307107748606 1104 76-77 -0.00496819322585651 1104 78-79 0.13640114195781905 1104 80-81 0.17447282705417422 1104 82-83 -0.141484160931185 1104 84-85 -0.13138284580301018 1104 86-87 0.09756358335831194 1104 88-89 0.10581833859996692 1104 90-91 0.34743201805809676 1104 92-93 0.36683641882195417 1104 94-95 0.42044936106776376 1104 96-97 0.3482078419272163 1104 98-99 0.2493733125976405 1104 100-101 0.03164143622984028 1105 1 -0.22777203934963097 1105 2 -1.7065520996169248 1105 3 -0.3658829159365773 1105 4 0.40852256483066895 1105 5 0.49872428649470635 1105 6 -0.02039131580564657 1105 7 -0.1190229050132885 1105 8 -0.11276689246425775 1105 9 0.39187381358122053 1105 10-11 0.8501160506348597 1105 12-13 0.9658637427318553 1105 14-15 1.1237019888043207 1105 16-17 0.7518170920665739 1105 18-19 0.5480457953198652 1105 20-21 0.982266048111164 1105 22-23 0.3041579594150008 1105 24-25 -0.14756527846407863 1105 26-27 0.91409684946629 1105 28-29 0.470525406564299 1105 30-31 0.9370549925238194 1105 32-33 0.9752748874391983 1105 34-35 0.51697148683607 1105 36-37 0.6966279027524607 1105 38-39 0.5007263486665039 1105 40-41 0.6548702975520939 1105 42-43 1.1049820086637183 1105 44-45 1.082325322904694 1105 46-47 1.1463865978642644 1105 48-49 1.0096628890764023 1105 50-51 0.7485187748180309 1105 52-53 0.843160250910401 1105 54-55 0.043192219380436825 1105 56-57 0.2055812512862829 1105 58-59 -0.2812477124793631 1105 60-61 0.4268774143238119 1105 62-63 1.024962067575899 1105 64-65 1.196613841028345 1105 66-67 0.8795397975205574 1105 68-69 0.8643434362699693 1105 70-71 0.5009643822300234 1105 72-73 -0.17206186468514417 1105 74-75 -0.22815984715600734 1105 76-77 0.09276851722695767 1105 78-79 -0.4392103300367296 1105 80-81 -0.21230384953141623 1105 82-83 -0.24237791833085964 1105 84-85 -0.23104874073733228 1105 86-87 -0.5697673703693695 1105 88-89 -0.7760363485322443 1105 90-91 -0.9877647736901096 1105 92-93 -0.7012792075284331 1105 94-95 -0.8416435032771474 1105 96-97 -1.0777888521541001 1105 98-99 -1.594611742260934 1105 100-101 -0.9456523303041457 1106 1 0.34557010525935894 1106 2 0.41704553729607596 1106 3 -0.3066786365705738 1106 4 0.44795905946931924 1106 5 0.1325982297487016 1106 6 0.4072920471876884 1106 7 0.45261755331180353 1106 8 0.6141122161399153 1106 9 0.8752870381265225 1106 10-11 0.25053677170247823 1106 12-13 0.37781682789248805 1106 14-15 0.12224323702211137 1106 16-17 -0.16990762841825102 1106 18-19 0.4660835866730153 1106 20-21 0.59073833248641 1106 22-23 0.5553566051149943 1106 24-25 0.46530150823019056 1106 26-27 0.7952780807553381 1106 28-29 0.6988143539637548 1106 30-31 0.4237004288727704 1106 32-33 0.42619039285605 1106 34-35 -0.9019089261144231 1106 36-37 -0.6788679049535915 1106 38-39 -0.5900807503077203 1106 40-41 0.5787792083882266 1106 42-43 0.8252754172051056 1106 44-45 0.6704790137537984 1106 46-47 0.493567856905365 1106 48-49 0.33873857238389604 1106 50-51 1.1466964711897916 1106 52-53 0.4623891949102372 1106 54-55 1.04295216415386 1106 56-57 0.7679219978857859 1106 58-59 1.3902397973639395 1106 60-61 1.547424337835693 1106 62-63 1.4739176885800447 1106 64-65 1.4208535909823752 1106 66-67 1.1244092761636182 1106 68-69 1.2783901013408894 1106 70-71 1.5211713778278515 1106 72-73 0.8059245159966508 1106 74-75 0.9613851233054049 1106 76-77 0.9482112838287549 1106 78-79 0.9709284995899701 1106 80-81 0.9319702088935848 1106 82-83 0.8472717120465347 1106 84-85 0.886164953511404 1106 86-87 0.7645094632605662 1106 88-89 0.896260306998915 1106 90-91 1.0025904525411278 1106 92-93 0.4611358104930545 1106 94-95 0.6647616003247236 1106 96-97 0.9995153484654651 1106 98-99 1.0825504719363188 1106 100-101 0.9510475000614527 1107 1 0.583578050205432 1107 2 0.5415047572949128 1107 3 0.23404624719094969 1107 4 -0.9421749255400158 1107 5 0.21196136456417491 1107 6 0.4194131150476821 1107 7 0.6119542402375515 1107 8 0.5612387587130883 1107 9 0.7706499825171917 1107 10-11 0.9368397269896818 1107 12-13 0.9938056880413342 1107 14-15 1.2612812743771187 1107 16-17 1.0615931835319614 1107 18-19 0.948019677992292 1107 20-21 0.29674523009605025 1107 22-23 0.4768389564381792 1107 24-25 0.6039793782229452 1107 26-27 1.1007543279055767 1107 28-29 1.0157750682683613 1107 30-31 1.2960776709926165 1107 32-33 1.3742272717934725 1107 34-35 1.0642692480290563 1107 36-37 1.3092657203644436 1107 38-39 1.166564652255616 1107 40-41 0.5143952590835426 1107 42-43 0.6114735330574419 1107 44-45 0.21261367582911106 1107 46-47 0.6726441379089039 1107 48-49 1.038006336109028 1107 50-51 -0.4930857568862237 1107 52-53 1.0192148133941359 1107 54-55 1.3331891901415531 1107 56-57 1.1293795410884115 1107 58-59 1.3756203560491684 1107 60-61 1.7970469264493474 1107 62-63 1.8186420222254611 1107 64-65 1.5867016425663394 1107 66-67 1.3559321456538598 1107 68-69 1.4057368490438975 1107 70-71 1.7244735026513176 1107 72-73 1.3953958755768738 1107 74-75 1.2557703421087965 1107 76-77 1.4511046300686559 1107 78-79 1.405366984443262 1107 80-81 1.4389215077494484 1107 82-83 1.198980563097308 1107 84-85 1.320579370785051 1107 86-87 1.3600091432411006 1107 88-89 1.3028129303650928 1107 90-91 1.4218294938580236 1107 92-93 1.2949614977414718 1107 94-95 1.4019137973509217 1107 96-97 1.6616448796501722 1107 98-99 1.6509205942842549 1107 100-101 1.607708083713483 1108 1 0.5538190996367618 1108 2 0.47478449767121234 1108 3 0.24511948330408373 1108 4 0.6908810320452687 1108 5 0.4201165087450818 1108 6 0.5002938028602912 1108 7 0.4324522089555103 1108 8 0.515859888206947 1108 9 0.6344580939746933 1108 10-11 0.7108623489631825 1108 12-13 0.9028611641815729 1108 14-15 1.1264915202409966 1108 16-17 0.8077413259564779 1108 18-19 0.7541405682495679 1108 20-21 0.6204274228702147 1108 22-23 0.6857635211316193 1108 24-25 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1.4729485187875255 2311 76-77 1.4207947381103203 2311 78-79 1.3098488970444748 2311 80-81 1.136871403489991 2311 82-83 1.4562687720740755 2311 84-85 1.2524922208256086 2311 86-87 1.1405006805580271 2311 88-89 1.1925566859948553 2311 90-91 1.4983855476250483 2311 92-93 1.3313092316534139 2311 94-95 1.6708484597642652 2311 96-97 1.7402674604077077 2311 98-99 2.1771223151874075 2311 100-101 2.097735007039237 2312 1 -0.024845577062329482 2312 2 -0.0844691465060663 2312 3 -0.18176100655828975 2312 4 0.2668551231393792 2312 5 -1.086909335270569 2312 6 -0.04835205566717349 2312 7 0.25507825826030484 2312 8 -2.522325662504784 2312 9 -4.541664064864836 2312 10-11 -0.47117278615600355 2312 12-13 -5.54638640390894 2312 14-15 -1.8795786674097954 2312 16-17 -2.450124551802098 2312 18-19 -2.453394369249331 2312 20-21 -4.0049824180307 2312 22-23 -2.2177971836944437 2312 24-25 -2.189410853042787 2312 26-27 -3.012851401295464 2312 28-29 -6.068944909378715 2312 30-31 -6.360996917858159 2312 32-33 -1.9220774749739817 2312 34-35 0.19576644478676286 2312 36-37 0.5379756813170289 2312 38-39 0.4763257342945053 2312 40-41 -2.694911744037725 2312 42-43 -4.96802116724907 2312 44-45 -3.382118082034779 2312 46-47 -0.2583268103072882 2312 48-49 -1.7308026966073982 2312 50-51 -7.204822517481048 2312 52-53 -1.8843938082081664 2312 54-55 0.320318004495725 2312 56-57 0.733179187541495 2312 58-59 0.7721748883436703 2312 60-61 1.1398624684727068 2312 62-63 1.3442432836493907 2312 64-65 1.357292099607868 2312 66-67 0.9473120176300043 2312 68-69 1.2666302500163553 2312 70-71 1.523623263045355 2312 72-73 0.9091983161457478 2312 74-75 0.7078716154673934 2312 76-77 1.1381120618550256 2312 78-79 0.9089119310953109 2312 80-81 0.9125132523307613 2312 82-83 0.18046392968389569 2312 84-85 0.4249789096857377 2312 86-87 0.850737663300464 2312 88-89 0.8730547547682548 2312 90-91 1.290590998524479 2312 92-93 1.3695657933785768 2312 94-95 1.4726779388763944 2312 96-97 1.6509889785197487 2312 98-99 1.8011752238813834 2312 100-101 1.7702266350978313 2313 1 -0.38126469142785524 2313 2 -0.1670655530582401 2313 3 -0.13047090258962868 2313 4 -1.2120122961568072 2313 5 -1.650257489785517 2313 6 -1.9140853735732577 2313 7 -1.7348885180500488 2313 8 -3.2172297105055776 2313 9 -1.6945591540398652 2313 10-11 -1.769648794023901 2313 12-13 -4.033601068614146 2313 14-15 -2.4238355972764083 2313 16-17 -4.284196189349821 2313 18-19 -2.81248294922586 2313 20-21 -4.142959905261897 2313 22-23 -2.14151493109523 2313 24-25 -0.01910225290063039 2313 26-27 -2.2164250140824464 2313 28-29 -3.116730823195468 2313 30-31 -2.915650713788672 2313 32-33 -6.747644839723815 2313 34-35 -1.6066352478031973 2313 36-37 -3.2141689822311292 2313 38-39 -1.2292313823520544 2313 40-41 -0.6841512355995718 2313 42-43 -0.9046978065323614 2313 44-45 -2.9791940667617958 2313 46-47 0.3603528372901934 2313 48-49 0.9256792287136832 2313 50-51 1.9959411315048854 2313 52-53 1.923685664179807 2313 54-55 -1.7095915807866717 2313 56-57 -3.2993337465565844 2313 58-59 0.23899252169298535 2313 60-61 -6.552793608487242 2313 62-63 -0.9594642156481186 2313 64-65 -0.8473972341001286 2313 66-67 -1.7672452477793428 2313 68-69 -3.0880698276708785 2313 70-71 -4.035402505975622 2313 72-73 0.24503031336884362 2313 74-75 2.724252051507662 2313 76-77 1.2720894829694203 2313 78-79 2.636039652214663 2313 80-81 3.3838102985629277 2313 82-83 3.4715596502743225 2313 84-85 3.153511612705625 2313 86-87 2.7944640058846417 2313 88-89 -1.4880338582666717 2313 90-91 2.620844391142537 2313 92-93 3.6354131627565813 2313 94-95 3.4743225941599576 2313 96-97 3.646691386824017 2313 98-99 4.534319573374763 2313 100-101 4.376046566005783 2314 1 -28.737700334992212 2314 2 -14.74469831633457 2314 3 -4.624620317531122 2314 4 -4.3906801629434895 2314 5 -1.7074132053570708 2314 6 -0.4539143564715431 2314 7 0.07496746754851102 2314 8 -5.486356623196844 2314 9 -3.453335031627624 2314 10-11 -0.6872905582003241 2314 12-13 0.640003791871905 2314 14-15 -1.6768734010567883 2314 16-17 -1.1357938491157924 2314 18-19 -3.483387539684905 2314 20-21 -2.415799689240295 2314 22-23 -5.042505030105133 2314 24-25 -1.0315910017755243 2314 26-27 -3.6981981914001807 2314 28-29 -3.1831124613592863 2314 30-31 -6.647648913608656 2314 32-33 -3.9965197272125614 2314 34-35 -2.7628008643648556 2314 36-37 -5.378210386371542 2314 38-39 -5.689177376951513 2314 40-41 -5.251995520028007 2314 42-43 -5.960053342085921 2314 44-45 -5.195215668922014 2314 46-47 -7.169125110504584 2314 48-49 -7.281341473356527 2314 50-51 -3.186439606568925 2314 52-53 -5.10961766615323 2314 54-55 -4.018997521380733 2314 56-57 -1.8996937825601847 2314 58-59 -1.4559894765068364 2314 60-61 -0.38605193431982343 2314 62-63 0.9171109418676338 2314 64-65 -2.5399039847751936 2314 66-67 -1.6937754007946424 2314 68-69 -0.4013011508031923 2314 70-71 0.38932746702167975 2314 72-73 -0.27870956061855523 2314 74-75 0.09835196149865766 2314 76-77 -2.1788556115400297 2314 78-79 -2.701044056156171 2314 80-81 -1.2606541478817164 2314 82-83 -2.0751325189426026 2314 84-85 -0.2776315016058071 2314 86-87 1.5261246719512442 2314 88-89 1.109850930212179 2314 90-91 0.9184366503684629 2314 92-93 1.0109757190122117 2314 94-95 1.3049931612166574 2314 96-97 1.3114078584711848 2314 98-99 1.348450986516081 2314 100-101 0.1308845498041613 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 11.0 4 44.0 5 95.0 6 214.0 7 598.0 8 1490.0 9 2630.0 10 4028.0 11 5939.0 12 7687.0 13 9644.0 14 11695.0 15 14075.0 16 16151.0 17 18108.0 18 16773.0 19 14664.0 20 14189.0 21 15071.0 22 16875.0 23 19096.0 24 22775.0 25 28078.0 26 35408.0 27 44609.0 28 57108.0 29 71141.0 30 87226.0 31 106412.0 32 127568.0 33 154608.0 34 189523.0 35 232051.0 36 290001.0 37 356934.0 38 321221.0 39 74455.0 40 88.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.76527893558247 21.410983574471047 15.414624005807253 23.40911348413923 2 14.68268091395936 21.92836715809219 40.485703160001506 22.90324876794694 3 18.007238169094116 26.402484242138236 31.180069450580007 24.410208138187645 4 12.50034020313372 18.376914924931302 39.15865776150944 29.96408711042554 5 12.049514987101634 38.56303702193246 36.40550737830085 12.98194061266506 6 29.544527674612382 39.42779143236374 18.101821805559627 12.925859087464254 7 25.709691871590135 33.27502104015844 23.255132333175617 17.760154755075806 8 24.291606128234008 37.0475109806598 20.717249580243603 17.943633310862584 9 24.762235741890642 16.468058736585593 21.46883334938387 37.3008721721399 10-11 23.351477416896607 27.390706321259145 29.589057442772866 19.668758819071385 12-13 24.211988493859625 24.896306562435885 29.45513317059486 21.43657177310963 14-15 21.45244086773382 26.68947238400697 27.07845362162886 24.77963312663035 16-17 21.00802247633244 29.672631883062778 27.7870568482052 21.532288792399584 18-19 20.846274949859524 28.714456787073594 29.752437936766473 20.686830326300406 20-21 21.61167613648259 27.852898936058857 29.799605575537306 20.73581935192125 22-23 21.30863504850751 27.747216627796455 29.622491405985034 21.321656917711 24-25 21.311497722316275 27.881005552083103 29.670026394119375 21.137470331481243 26-27 21.288704603850444 27.99888446321911 29.560970952494216 21.151439980436233 28-29 21.1505281680926 27.962080346871033 29.64893125911379 21.23846022592258 30-31 21.517331301733414 27.787928100958624 29.56702259443098 21.127718002876982 32-33 21.387732081441104 27.95702594086618 29.393081542433503 21.26216043525921 34-35 21.590408775570065 27.937125107269228 29.45450183218665 21.01796428497406 36-37 21.475728846417557 27.790572912904803 29.40516753182995 21.328530708847694 38-39 21.38500755563725 27.881348232181864 29.488883855054027 21.244760357126857 40-41 21.60617334790736 27.676245511127 29.471292253188665 21.24628888777697 42-43 21.442801651567216 27.857761055354967 29.292519251326375 21.40691804175144 44-45 21.609122536985776 27.91813198017153 29.140348945246437 21.332396537596257 46-47 21.610770664760732 27.881728916938048 28.946632722316888 21.560867695984328 48-49 21.543311815042788 27.9770022092 28.933924379655764 21.545761596101457 50-51 21.641877051162396 28.147176179841395 28.671913339790045 21.539033429206164 52-53 21.628237323655426 28.11616837799107 28.633470218349572 21.622124080003935 54-55 21.641648190958332 28.060974235654395 28.68871548554078 21.608662087846493 56-57 21.65973031010214 28.134377244702897 28.53640608450959 21.66948636068538 58-59 21.603118415719536 28.18926215862233 28.624054962097993 21.583564463560144 60-61 21.684242966426897 28.186507322545186 28.523161627773813 21.606088083254107 62-63 21.602427600853613 28.16630003791556 28.529753456733026 21.7015189044978 64-65 21.49239975017237 28.099536587750702 28.521073605560026 21.886990056516904 66-67 21.565848622503854 28.097207237970895 28.458029638354787 21.878914501170463 68-69 21.598824069559274 28.128788752705187 28.38810142965322 21.88428574808232 70-71 21.655787314349983 27.864701864989954 28.39940154838677 22.08010927227329 72-73 21.662261997654706 27.948687403051398 28.412428599034705 21.976622000259194 74-75 21.560029460667955 28.122125271483238 28.271626856103733 22.046218411745073 76-77 21.508275795652956 28.131738607957995 28.211879629358243 22.148105967030805 78-79 21.522876837769633 28.11789109274464 28.110563671314488 22.248668398171244 80-81 21.50796492043454 28.208023749259404 28.036080283550746 22.247931046755305 82-83 21.67076480852832 28.09767709154401 28.175034098843206 22.056524001084462 84-85 21.690360756173142 28.130892031910708 27.967909243814294 22.210837968101853 86-87 21.514541268528962 28.11875786269847 28.07041711844362 22.296283750328953 88-89 21.606783501925715 28.022436088009155 28.03954097762086 22.33123943244427 90-91 21.651397889217744 28.003089276446484 28.01280345556799 22.332709378767778 92-93 21.58652987572117 27.974073561527142 28.03177347466323 22.407623088088464 94-95 21.44283307586003 27.99346473509001 27.986618785495043 22.577083403554912 96-97 21.406083627582877 27.96233187161132 28.030309691623128 22.601274809182677 98-99 21.3886452770723 27.96848820380291 27.893035419052918 22.749831100071873 100-101 21.44267934778285 27.846583003346005 27.907046368785494 22.80369128008565 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 259.0 1 183.5 2 122.0 3 156.5 4 144.0 5 136.5 6 248.0 7 478.5 8 821.0 9 1107.0 10 1146.0 11 1141.5 12 1251.5 13 1389.5 14 1558.0 15 1786.0 16 2023.0 17 2227.0 18 2453.5 19 2782.0 20 3255.0 21 3957.5 22 5012.0 23 6603.5 24 8706.0 25 11474.0 26 15252.5 27 19826.0 28 24969.0 29 30878.0 30 37319.0 31 43926.5 32 50614.0 33 57016.5 34 63173.5 35 69209.5 36 75840.0 37 81656.0 38 84857.0 39 86306.5 40 86913.5 41 87946.5 42 88787.0 43 88992.0 44 89194.0 45 89856.0 46 88982.0 47 87016.5 48 85735.0 49 83150.5 50 79592.0 51 76020.0 52 71491.0 53 67064.5 54 63245.0 55 57616.0 56 51710.5 57 45899.0 58 39812.5 59 34461.0 60 28734.0 61 22832.5 62 17731.5 63 13626.5 64 10248.0 65 7452.0 66 5484.5 67 4172.5 68 3233.0 69 2564.0 70 1946.5 71 1448.0 72 1099.0 73 827.0 74 600.0 75 452.0 76 347.0 77 227.0 78 152.5 79 122.0 80 98.0 81 71.0 82 51.5 83 46.0 84 32.5 85 22.5 86 17.5 87 11.5 88 8.5 89 4.0 90 3.0 91 1.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09668005141754142 2 0.0 3 0.004898902562084169 4 4.6058058276005005E-4 5 1.256128862072864E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.01873725552592022 26-27 0.008457934337957282 28-29 0.0034543543707003755 30-31 0.0010258385706928387 32-33 5.024515448291456E-4 34-35 9.839676086237433E-4 36-37 5.233870258636933E-4 38-39 2.9309673448366824E-4 40-41 9.839676086237433E-4 42-43 5.44322506898241E-4 44-45 2.9309673448366824E-4 46-47 0.02748828659836117 48-49 0.013461514305214192 50-51 0.08972947171407157 52-53 0.0013608062672456026 54-55 0.03856315606563692 56-57 0.0011933224189692208 58-59 0.001088645013796482 60-61 0.003496225332769471 62-63 0.0037055801431149483 64-65 0.010195579263824745 66-67 0.005631644398293339 68-69 0.002240096470696607 70-71 0.005317612182775123 72-73 0.004375515536220476 74-75 8.792902034510048E-4 76-77 0.0 78-79 4.187096206909546E-5 80-81 2.093548103454773E-4 82-83 5.233870258636933E-4 84-85 4.187096206909546E-5 86-87 0.001486419153452889 88-89 0.003517160813804019 90-91 0.0011933224189692208 92-93 0.0029937737879403253 94-95 6.908708741400751E-4 96-97 4.6058058276005005E-4 98-99 0.0017376449258674615 100-101 0.0028053544586293957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2388290.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.75621243653856 #Duplication Level Percentage of deduplicated Percentage of total 1 84.01120161103329 60.283256298501755 2 10.436905146795764 14.978255657869589 3 2.681633625784781 5.772716163863337 4 1.048101511767664 3.008311789338309 5 0.5055371375991564 1.8137715120062348 6 0.30289088898135696 1.304058178490296 7 0.1980209204162277 0.9946461862586014 8 0.13983715603805297 0.8027347740150336 9 0.098190519912625 0.6341201825494039 >10 0.5345756553841723 6.835608439013234 >50 0.031224911572315998 1.4967575330510492 >100 0.011470440705110361 1.3434091160808421 >500 1.7591743266237507E-4 0.08373368550688125 >1k 1.7591743266024386E-4 0.39056083862687113 >5k 5.8639144220081284E-5 0.25805964482854993 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6133 0.2567946103697624 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4849 0.2030322950730439 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3287 0.13762985232111677 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.187096206909546E-5 2 0.0 0.0 0.0 0.0 4.187096206909546E-5 3 0.0 0.0 0.0 0.0 4.187096206909546E-5 4 0.0 0.0 0.0 0.0 4.187096206909546E-5 5 0.0 0.0 0.0 0.0 4.187096206909546E-5 6 8.374192413819092E-5 0.0 0.0 0.0 4.187096206909546E-5 7 8.374192413819092E-5 0.0 0.0 4.187096206909546E-5 4.187096206909546E-5 8 8.374192413819092E-5 0.0 0.0 8.374192413819092E-5 4.187096206909546E-5 9 8.374192413819092E-5 8.374192413819092E-5 0.0 1.256128862072864E-4 4.187096206909546E-5 10-11 8.374192413819092E-5 8.374192413819092E-5 0.0 1.256128862072864E-4 4.187096206909546E-5 12-13 8.374192413819092E-5 1.256128862072864E-4 0.0 1.256128862072864E-4 4.187096206909546E-5 14-15 8.374192413819092E-5 2.093548103454773E-4 0.0 1.256128862072864E-4 4.187096206909546E-5 16-17 8.374192413819092E-5 2.093548103454773E-4 0.0 1.6748384827638184E-4 4.187096206909546E-5 18-19 8.374192413819092E-5 2.3029029138002505E-4 0.0 1.6748384827638184E-4 4.187096206909546E-5 20-21 8.374192413819092E-5 2.512257724145728E-4 0.0 2.512257724145728E-4 4.187096206909546E-5 22-23 8.374192413819092E-5 2.9309673448366824E-4 0.0 3.349676965527637E-4 4.187096206909546E-5 24-25 8.374192413819092E-5 3.559031775873114E-4 0.0 4.187096206909546E-4 8.374192413819092E-5 26-27 8.374192413819092E-5 3.7683865862185914E-4 0.0 8.583547224164569E-4 8.374192413819092E-5 28-29 8.374192413819092E-5 3.7683865862185914E-4 0.0 0.00249132224311118 8.374192413819092E-5 30-31 8.374192413819092E-5 5.024515448291456E-4 0.0 0.007620515096575374 8.374192413819092E-5 32-33 8.374192413819092E-5 5.861934689673365E-4 0.0 0.02055864237592587 8.374192413819092E-5 34-35 8.374192413819092E-5 6.280644310364319E-4 0.0 0.044173864982895716 8.374192413819092E-5 36-37 8.374192413819092E-5 6.699353931055274E-4 0.0 0.08240205335197986 1.0467740517273866E-4 38-39 8.374192413819092E-5 6.699353931055274E-4 0.0 0.13256346591075624 1.256128862072864E-4 40-41 8.374192413819092E-5 7.118063551746228E-4 0.0 0.18322733001436173 1.256128862072864E-4 42-43 8.374192413819092E-5 7.118063551746228E-4 0.0 0.23420522633348545 1.256128862072864E-4 44-45 8.374192413819092E-5 8.164837603473615E-4 0.0 0.3008637979474854 1.256128862072864E-4 46-47 8.374192413819092E-5 9.211611655201001E-4 0.0 0.3832867867804999 1.256128862072864E-4 48-49 8.374192413819092E-5 9.211611655201001E-4 0.0 0.4680336140083491 1.256128862072864E-4 50-51 1.256128862072864E-4 0.0010467740517273866 0.0 0.5550833441499985 1.256128862072864E-4 52-53 1.256128862072864E-4 0.0010677095327619344 0.0 0.639118365022673 1.256128862072864E-4 54-55 1.256128862072864E-4 0.001088645013796482 0.0 0.7364055453902165 1.256128862072864E-4 56-57 1.256128862072864E-4 0.001088645013796482 0.0 0.8484731753681505 1.256128862072864E-4 58-59 1.256128862072864E-4 0.001088645013796482 0.0 0.9677426108219689 1.256128862072864E-4 60-61 1.256128862072864E-4 0.0011095804948310297 0.0 1.0916178521033877 1.256128862072864E-4 62-63 1.256128862072864E-4 0.0011305159758655775 0.0 1.215576835308945 1.256128862072864E-4 64-65 1.256128862072864E-4 0.0014236127103492457 0.0 1.3488521075748756 1.256128862072864E-4 66-67 1.256128862072864E-4 0.0014236127103492457 0.0 1.5041096349270817 1.256128862072864E-4 68-69 1.256128862072864E-4 0.001486419153452889 0.0 1.6742941602569203 1.4654836724183412E-4 70-71 1.256128862072864E-4 0.0015073546344874366 0.0 1.860808360793706 1.6748384827638184E-4 72-73 1.6748384827638184E-4 0.0015073546344874366 0.0 2.0518655607149885 1.6748384827638184E-4 74-75 1.6748384827638184E-4 0.0015910965586256275 0.0 2.245979340867315 1.8841932931092957E-4 76-77 1.6748384827638184E-4 0.0016539030017292708 0.0 2.461740408409364 2.093548103454773E-4 78-79 1.6748384827638184E-4 0.0016748384827638184 0.0 2.699106892379066 2.093548103454773E-4 80-81 1.6748384827638184E-4 0.0016748384827638184 0.0 2.9510026001867447 2.093548103454773E-4 82-83 1.6748384827638184E-4 0.0016748384827638184 0.0 3.2150827579565293 2.093548103454773E-4 84-85 1.6748384827638184E-4 0.0016748384827638184 0.0 3.4807330768039058 2.093548103454773E-4 86-87 1.6748384827638184E-4 0.0016748384827638184 0.0 3.76175003873064 2.093548103454773E-4 88-89 1.6748384827638184E-4 0.0016748384827638184 0.0 4.065691352390204 2.093548103454773E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2955 0.0 53.248028 1 GTATCAA 5465 0.0 42.18758 1 ATCAACG 6795 0.0 33.199215 3 TCAACGC 6915 0.0 32.689716 4 CAACGCA 6925 0.0 32.573933 5 AACGCAG 7215 0.0 31.330477 6 TATCAAC 7375 0.0 30.845877 2 ACGCAGA 8410 0.0 26.426899 7 CGCAGAG 8370 0.0 26.212767 8 GTGGTAT 1525 0.0 24.625797 1 GCAGAGT 9415 0.0 22.44585 9 TGGTATC 1590 0.0 22.103357 2 GTACATG 5935 0.0 21.06532 1 AGAGTAC 8505 0.0 20.910955 10-11 GAGTACT 6185 0.0 19.963194 12-13 TACATGG 5985 0.0 19.758722 2 ACATGGG 5715 0.0 19.362583 3 CAGAGTA 9285 0.0 19.282166 10-11 GGACCGT 350 6.366463E-11 18.995745 6 AGTACTT 6735 0.0 18.297684 12-13 >>END_MODULE