FastQCFastQC Report
Sat 18 Jun 2016
SRR1931117_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931117_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435794
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49860.2046971131384674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38330.15736141890488275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.053.7075841
GTATCAA57000.041.4820441
ATCAACG64650.035.254013
TCAACGC67750.034.3510784
CAACGCA68400.034.0951045
AACGCAG71150.033.025826
TATCAAC71650.032.467032
ACGCAGA77400.029.5028847
CGCAGAG78950.028.6223758
GTACATG64650.025.4906161
GCAGAGT87150.024.4008149
TACATGG66350.023.9015982
ACATGGG65900.022.3574493
GAGTACT52800.021.7072212-13
CAGAGTA86050.021.17401110-11
AGAGTAC80100.020.5196910-11
GTACTTT60050.019.23316214-15
GTACTAG8100.018.8709931
AGTACTT58950.018.35349312-13
GTGGTAT14200.017.1558251
Produced by FastQC (version 0.11.5)