##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931117_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2435794 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.507222285628423 31.0 28.0 31.0 22.0 34.0 2 28.207168997049834 31.0 28.0 33.0 16.0 34.0 3 28.241822584340056 31.0 28.0 33.0 16.0 34.0 4 31.43280999953198 35.0 32.0 37.0 17.0 37.0 5 31.48820754136023 35.0 32.0 37.0 17.0 37.0 6 31.745279773248477 35.0 32.0 37.0 19.0 37.0 7 31.413128942759528 35.0 32.0 37.0 17.0 37.0 8 31.566402577557874 35.0 32.0 37.0 17.0 37.0 9 32.54104657454612 37.0 32.0 39.0 17.0 39.0 10-11 32.52539377303664 37.0 31.5 39.0 17.0 39.0 12-13 32.590699172425914 37.0 32.0 39.0 17.0 39.0 14-15 33.25812240279761 38.0 31.0 40.0 17.0 41.0 16-17 33.256491517755606 37.5 31.0 40.0 17.0 41.0 18-19 33.300173167353236 37.5 31.5 40.0 17.5 41.0 20-21 33.497525858098015 38.0 32.0 40.0 18.0 41.0 22-23 33.38409549411814 38.0 32.0 40.0 18.0 41.0 24-25 33.2762187606998 37.5 31.5 40.0 17.5 41.0 26-27 32.874192563082104 37.0 31.0 40.0 16.5 41.0 28-29 32.829538130071754 37.0 31.0 40.0 16.0 41.0 30-31 32.57741089763749 37.0 30.5 40.0 14.5 41.0 32-33 32.36645217124273 37.0 30.0 40.0 15.0 41.0 34-35 32.37127564974706 36.0 30.0 40.0 15.0 41.0 36-37 32.01955276185096 36.0 30.0 40.0 9.0 41.0 38-39 31.817709748853964 36.0 30.0 40.0 9.0 41.0 40-41 31.64531709167524 36.0 30.0 40.0 9.0 41.0 42-43 31.870662707930144 36.5 30.0 40.0 9.0 41.0 44-45 31.948066010508278 37.0 30.0 40.0 8.5 41.0 46-47 31.706982199644138 36.0 30.0 40.0 7.5 41.0 48-49 31.468068728307895 36.0 30.0 40.0 4.0 41.0 50-51 30.92354546402528 35.5 29.0 39.5 2.0 40.5 52-53 30.84914118353194 35.5 29.0 39.0 2.0 40.5 54-55 30.83198887098006 35.5 29.0 40.0 2.0 41.0 56-57 30.573943034591593 35.0 28.0 39.5 2.0 41.0 58-59 30.418695300177273 35.0 28.0 39.0 2.0 41.0 60-61 30.244591907197407 35.0 28.0 39.0 2.0 40.5 62-63 30.09725760881257 35.0 28.0 39.0 2.0 40.0 64-65 29.719689555028054 34.0 28.0 39.0 2.0 40.0 66-67 29.494131482383157 34.0 28.0 38.0 2.0 40.0 68-69 29.295426665801784 34.0 28.0 38.0 2.0 40.0 70-71 28.980825143669783 34.0 27.5 37.5 2.0 40.0 72-73 28.62564650376838 34.0 26.5 37.0 2.0 39.5 74-75 28.20020822778938 33.5 26.0 36.0 2.0 39.0 76-77 27.892774594239086 33.0 26.0 36.0 2.0 39.0 78-79 27.473810798450117 33.0 26.0 35.5 2.0 38.5 80-81 27.07448515761185 33.0 25.5 35.0 2.0 37.0 82-83 26.75062792666375 32.5 25.0 35.0 2.0 37.0 84-85 26.41403070210371 32.0 25.0 35.0 2.0 36.0 86-87 26.092839747532018 32.0 24.0 35.0 2.0 36.0 88-89 25.747027457987006 32.0 23.0 35.0 2.0 36.0 90-91 25.373417251212544 32.0 20.0 35.0 2.0 35.0 92-93 25.007080032219474 32.0 18.5 35.0 2.0 35.0 94-95 24.68852476851491 32.0 16.5 34.0 2.0 35.0 96-97 24.289824796349773 31.5 4.5 34.0 2.0 35.0 98-99 23.915629770005182 31.0 2.0 34.0 2.0 35.0 100-101 23.324183818500252 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.13578755221398 1101 2 5.825100685354776 1101 3 5.348250247730029 1101 4 6.678026160556012 1101 5 6.137969763241319 1101 6 5.647294262630435 1101 7 6.204301608866473 1101 8 5.653516507387042 1101 9 6.418140097646656 1101 10-11 6.345464335689389 1101 12-13 6.16437077261487 1101 14-15 6.430087292347121 1101 16-17 6.392788428441783 1101 18-19 6.522433485935892 1101 20-21 5.766841822322835 1101 22-23 5.8703787865667145 1101 24-25 5.788740337599311 1101 26-27 5.972761177571339 1101 28-29 5.895474549457898 1101 30-31 6.03325993729711 1101 32-33 5.7491538004119 1101 34-35 5.778430574237422 1101 36-37 5.724286270565962 1101 38-39 5.611001895525568 1101 40-41 5.76748429395812 1101 42-43 5.586948940235221 1101 44-45 5.5775165881624105 1101 46-47 5.299924470267445 1101 48-49 5.233500451780145 1101 50-51 5.255060753484237 1101 52-53 5.245295060066187 1101 54-55 5.4485183648078035 1101 56-57 5.268233384801885 1101 58-59 5.117097011344484 1101 60-61 4.969666535304853 1101 62-63 5.026348355007194 1101 64-65 4.965070534837928 1101 66-67 4.873098927478242 1101 68-69 5.068486855478735 1101 70-71 4.879669811051677 1101 72-73 4.663076001954725 1101 74-75 4.775952718922106 1101 76-77 4.6522930287217825 1101 78-79 4.571606642004543 1101 80-81 4.470477813020967 1101 82-83 4.42433195247278 1101 84-85 4.414922112722557 1101 86-87 4.257762896686874 1101 88-89 4.356024542785242 1101 90-91 4.341901218053852 1101 92-93 4.253055216473875 1101 94-95 4.222145795676216 1101 96-97 4.008818562357224 1101 98-99 3.814342985628276 1101 100-101 3.79326710324332 1102 1 5.408684643204669 1102 2 5.937501093405654 1102 3 5.658456965111757 1102 4 6.976904492711714 1102 5 6.574381925809984 1102 6 5.919791075556322 1102 7 6.560241028329337 1102 8 6.141404033861821 1102 9 6.808916828119209 1102 10-11 6.6652212968608815 1102 12-13 6.719415319194017 1102 14-15 7.172415451634517 1102 16-17 6.994059410104519 1102 18-19 7.0479487972854855 1102 20-21 6.224053787892334 1102 22-23 6.498144531239813 1102 24-25 6.245124410440699 1102 26-27 6.342852748589184 1102 28-29 6.240440492886723 1102 30-31 6.529922621676427 1102 32-33 6.2291241429471675 1102 34-35 6.389094916158765 1102 36-37 6.315542917531253 1102 38-39 6.076680109947262 1102 40-41 6.376638288812252 1102 42-43 6.252508739977632 1102 44-45 6.27480619186737 1102 46-47 6.328658144609296 1102 48-49 6.002161769768676 1102 50-51 5.894010240939487 1102 52-53 5.706115868333658 1102 54-55 6.11604856790666 1102 56-57 6.004107911132191 1102 58-59 5.973878485869626 1102 60-61 5.843953862496932 1102 62-63 5.738978374017655 1102 64-65 5.856719154592209 1102 66-67 5.730196364776429 1102 68-69 5.784312237411637 1102 70-71 5.570904056278312 1102 72-73 5.327982907113636 1102 74-75 5.3913337219403275 1102 76-77 5.385100805885248 1102 78-79 5.305958662519508 1102 80-81 5.204958447291535 1102 82-83 4.939915432955331 1102 84-85 4.956911594609483 1102 86-87 4.885134785749031 1102 88-89 5.038987463379119 1102 90-91 5.133054463511915 1102 92-93 5.1241820102048585 1102 94-95 5.048342913630236 1102 96-97 4.995327676532863 1102 98-99 4.623138162925333 1102 100-101 4.568603299490672 1103 1 5.0203136624053 1103 2 5.562141626609389 1103 3 5.235275854928684 1103 4 6.658475171729389 1103 5 6.134213256929311 1103 6 5.497654228311955 1103 7 6.059722271875316 1103 8 5.584241177974757 1103 9 6.22130248149341 1103 10-11 6.195754230592396 1103 12-13 6.351148751409763 1103 14-15 6.5898611105680835 1103 16-17 6.498032605400837 1103 18-19 6.439243924560074 1103 20-21 5.759344276014719 1103 22-23 5.993693909595191 1103 24-25 5.649180913287434 1103 26-27 5.87319195090819 1103 28-29 5.743390837226816 1103 30-31 6.049643057538084 1103 32-33 5.698278771321458 1103 34-35 5.827082799521779 1103 36-37 5.759001617745348 1103 38-39 5.4302476284014105 1103 40-41 5.553824103656034 1103 42-43 5.361278872220684 1103 44-45 5.1647932517266355 1103 46-47 5.17325091636663 1103 48-49 4.980241958141008 1103 50-51 5.058746481831925 1103 52-53 4.914690681247151 1103 54-55 5.2121580494202036 1103 56-57 5.3123183773092215 1103 58-59 5.2723070787338955 1103 60-61 5.048020457192777 1103 62-63 5.099013749711084 1103 64-65 4.959433292121982 1103 66-67 5.105388740650007 1103 68-69 5.271233306094192 1103 70-71 5.03533595931335 1103 72-73 4.835719576467035 1103 74-75 4.968319841000248 1103 76-77 4.952495408029588 1103 78-79 4.932053965648997 1103 80-81 4.808679445950826 1103 82-83 4.602446958918161 1103 84-85 4.594891322607591 1103 86-87 4.675292073635401 1103 88-89 4.648039464730012 1103 90-91 4.600810369346107 1103 92-93 4.5786359847335625 1103 94-95 4.559307757478788 1103 96-97 4.539016530660941 1103 98-99 4.229050653093523 1103 100-101 4.314210357411245 1104 1 4.648287641545046 1104 2 5.541156102747934 1104 3 5.146960301765517 1104 4 6.522483123588859 1104 5 6.140459158098981 1104 6 5.71216065264036 1104 7 6.196411835395544 1104 8 5.7763687266152 1104 9 6.457038470703608 1104 10-11 6.355002153143115 1104 12-13 6.243646921779099 1104 14-15 6.420665591845786 1104 16-17 6.257526713673265 1104 18-19 6.423921685073729 1104 20-21 5.819348769055782 1104 22-23 5.917539544388614 1104 24-25 5.6932249827846455 1104 26-27 5.923122792412638 1104 28-29 5.93475560874111 1104 30-31 6.088609519445342 1104 32-33 5.9759568331223285 1104 34-35 5.873146489895358 1104 36-37 5.918365305427223 1104 38-39 5.497018437650851 1104 40-41 5.838924375756896 1104 42-43 5.851386680834683 1104 44-45 5.698080723112341 1104 46-47 5.732287017160875 1104 48-49 5.616378746544939 1104 50-51 5.4016457248985645 1104 52-53 5.343710678908561 1104 54-55 5.595018088862126 1104 56-57 5.648898875182152 1104 58-59 5.532504199537286 1104 60-61 5.256215420729873 1104 62-63 5.167249157116878 1104 64-65 5.409631809689227 1104 66-67 5.399537215401754 1104 68-69 5.364792708283598 1104 70-71 5.241801026216926 1104 72-73 5.09712127746436 1104 74-75 5.10063954838499 1104 76-77 5.056023934643033 1104 78-79 5.054728013987859 1104 80-81 4.90406872251976 1104 82-83 4.708015876432679 1104 84-85 4.8976410247459405 1104 86-87 4.674077419844092 1104 88-89 4.72981516806885 1104 90-91 4.704868545129191 1104 92-93 4.738603411269011 1104 94-95 4.671460772823313 1104 96-97 4.644756103580487 1104 98-99 4.380711856999977 1104 100-101 4.405450909554869 1105 1 4.489820884796249 1105 2 5.124529840791364 1105 3 4.862448248200767 1105 4 6.168046020788797 1105 5 3.67164672837335 1105 6 4.775206235677853 1105 7 5.516271095855238 1105 8 0.8346396888501033 1105 9 0.6394037045468721 1105 10-11 2.0867429752217284 1105 12-13 5.303517903943412 1105 14-15 3.353726789543547 1105 16-17 5.617280136090066 1105 18-19 6.144190908626456 1105 20-21 3.234597404494753 1105 22-23 5.219064249103607 1105 24-25 5.367246130490184 1105 26-27 5.555169943374761 1105 28-29 2.963871022057731 1105 30-31 5.327687149277914 1105 32-33 5.379984832877955 1105 34-35 5.523864175743785 1105 36-37 5.628262705253142 1105 38-39 5.40923495689772 1105 40-41 5.785215954905652 1105 42-43 2.8921436264191804 1105 44-45 4.957980558095045 1105 46-47 5.244594609294328 1105 48-49 5.0507693948574826 1105 50-51 5.130440798118364 1105 52-53 5.040905622139096 1105 54-55 5.251821162313377 1105 56-57 5.105445199410195 1105 58-59 5.004281028984245 1105 60-61 4.917428335746987 1105 62-63 4.715535416354246 1105 64-65 4.7951438855954684 1105 66-67 4.890718356071371 1105 68-69 4.937856869030046 1105 70-71 4.666598903261082 1105 72-73 4.553987467279658 1105 74-75 4.659515576233694 1105 76-77 4.53606642433942 1105 78-79 4.4151529663111795 1105 80-81 4.353570260095548 1105 82-83 4.247527992276808 1105 84-85 4.199483617924955 1105 86-87 4.149133637695659 1105 88-89 4.1385180757119535 1105 90-91 4.168928135359852 1105 92-93 4.236335453206955 1105 94-95 4.0577624787972795 1105 96-97 4.022451952300511 1105 98-99 4.002248620848622 1105 100-101 4.034542228329677 1106 1 4.283398385857662 1106 2 4.6130535046236645 1106 3 4.658203193905219 1106 4 6.31161262401103 1106 5 6.236619118439709 1106 6 5.224112062606405 1106 7 6.094981498939113 1106 8 5.683902329410085 1106 9 6.345765838900938 1106 10-11 6.200382435459108 1106 12-13 6.46385517248147 1106 14-15 6.3024261176114145 1106 16-17 6.188413557489554 1106 18-19 6.432564572506351 1106 20-21 5.809316753104877 1106 22-23 5.797971816318604 1106 24-25 5.890203598314098 1106 26-27 6.18507775065704 1106 28-29 6.1002994124919105 1106 30-31 6.278689922534554 1106 32-33 5.987376540314205 1106 34-35 5.7051781190988855 1106 36-37 5.837792497719768 1106 38-39 5.64235016993711 1106 40-41 5.853604636352038 1106 42-43 2.98693432750569 1106 44-45 5.058314697485329 1106 46-47 5.351996346989015 1106 48-49 5.125007620523803 1106 50-51 5.407398966303248 1106 52-53 5.200948828392466 1106 54-55 5.533075539111827 1106 56-57 5.466207933939781 1106 58-59 5.3141656070665455 1106 60-61 5.029962161329287 1106 62-63 5.036402724747575 1106 64-65 5.177939144372843 1106 66-67 5.329234921848521 1106 68-69 5.4258149214642835 1106 70-71 5.245995390664582 1106 72-73 5.027537712395013 1106 74-75 5.217166866026091 1106 76-77 5.083938285371872 1106 78-79 5.244326060823816 1106 80-81 5.209310580658215 1106 82-83 5.182006097796737 1106 84-85 5.171347132318118 1106 86-87 5.117385453703232 1106 88-89 5.290285257160281 1106 90-91 5.27480816570834 1106 92-93 5.397546489039069 1106 94-95 5.293346380042134 1106 96-97 5.332125382460735 1106 98-99 5.263820189561276 1106 100-101 5.1922867848828 1107 1 2.6569197087038603 1107 2 -0.40628772410347125 1107 3 0.37595923224480643 1107 4 2.120378769324809 1107 5 1.9850913669902646 1107 6 3.5169139122541573 1107 7 4.444213350254611 1107 8 4.105896729502067 1107 9 4.4033128181514485 1107 10-11 2.248228086829098 1107 12-13 4.445910864944377 1107 14-15 2.6185659930792333 1107 16-17 1.2356109102226434 1107 18-19 1.7764470407336574 1107 20-21 1.3779985993964132 1107 22-23 3.666040117379435 1107 24-25 3.613890556619335 1107 26-27 2.4194733676879707 1107 28-29 3.432509242267553 1107 30-31 2.7675766533629513 1107 32-33 3.440225283753275 1107 34-35 3.9862559511907314 1107 36-37 4.134698667666516 1107 38-39 4.095787183402376 1107 40-41 2.628380551982577 1107 42-43 3.2373207584499966 1107 44-45 3.7823466001799346 1107 46-47 4.102538676223414 1107 48-49 1.3032230357052939 1107 50-51 3.048082411976573 1107 52-53 3.471014647511087 1107 54-55 4.057151880588123 1107 56-57 2.710591244846551 1107 58-59 1.6553554373186863 1107 60-61 2.790737581814426 1107 62-63 3.8248340357537884 1107 64-65 3.957575616492946 1107 66-67 4.130082055547103 1107 68-69 4.05700157159848 1107 70-71 3.792066028645962 1107 72-73 3.6726521914616512 1107 74-75 3.762666015059679 1107 76-77 3.6192511644351946 1107 78-79 3.635050837671727 1107 80-81 3.6800774241477257 1107 82-83 3.5276772010904907 1107 84-85 3.5477625696672064 1107 86-87 3.518033648753228 1107 88-89 3.599099829351548 1107 90-91 3.6383529820643012 1107 92-93 3.6856534176562974 1107 94-95 3.626017844854182 1107 96-97 3.6317203058737597 1107 98-99 3.5828652115372357 1107 100-101 3.655734747483784 1108 1 -2.032161061799453 1108 2 -1.5481492088905746 1108 3 -2.127684814807438 1108 4 -3.5919803792588105 1108 5 -8.354455971728306 1108 6 -6.811066462022588 1108 7 -5.454582099461199 1108 8 -5.73888090750275 1108 9 -7.732476689510818 1108 10-11 -3.4813710868246552 1108 12-13 -2.0853883439474536 1108 14-15 -6.459069666773843 1108 16-17 -6.107603743435526 1108 18-19 -4.463766055455519 1108 20-21 -4.888028402112635 1108 22-23 -3.7325886912240342 1108 24-25 -8.346354038640467 1108 26-27 -9.101646073237152 1108 28-29 -6.100291350592837 1108 30-31 -5.76921068352647 1108 32-33 -2.7082717183206633 1108 34-35 -1.166530815433699 1108 36-37 -4.807543980084567 1108 38-39 -5.76170682478039 1108 40-41 -3.396701020119629 1108 42-43 -4.0255517631165105 1108 44-45 -1.8098006895819267 1108 46-47 -2.4889443355288883 1108 48-49 -1.3860730636284124 1108 50-51 -0.7138090924201137 1108 52-53 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40-41 -1.2023048268955705 2313 42-43 -2.7654331141833914 2313 44-45 -4.320870664483113 2313 46-47 -4.955083019350088 2313 48-49 -2.6606676920408567 2313 50-51 -3.041308117172992 2313 52-53 -1.7513473278629341 2313 54-55 -5.049646742475559 2313 56-57 -2.923203273887726 2313 58-59 -1.8035836155962706 2313 60-61 -0.7399963428954024 2313 62-63 -2.6119857709455374 2313 64-65 -4.105975290431459 2313 66-67 -5.760451507275615 2313 68-69 -6.582397784065936 2313 70-71 -5.623565729154471 2313 72-73 -3.7140065313416457 2313 74-75 -4.4220707341349055 2313 76-77 -3.500438583567803 2313 78-79 -3.6808589553464977 2313 80-81 -4.299789295478032 2313 82-83 -3.956143797188343 2313 84-85 -4.55438889332763 2313 86-87 -3.3019429609824194 2313 88-89 -5.379749634437676 2313 90-91 -4.863613124118675 2313 92-93 -4.078422666901716 2313 94-95 -4.75914478368194 2313 96-97 -4.899307100454855 2313 98-99 -4.493676118618552 2313 100-101 -3.7797191560424075 2314 1 -10.537942760039037 2314 2 -14.220298484124687 2314 3 -12.338195499891286 2314 4 -24.827412395333827 2314 5 -17.51601494505035 2314 6 -16.280085405772667 2314 7 -16.323991544797295 2314 8 -11.452411977022141 2314 9 -13.262952242089519 2314 10-11 -10.839450931129527 2314 12-13 -11.795124976534748 2314 14-15 -15.01101648778763 2314 16-17 -15.188307404545865 2314 18-19 -13.654268273352876 2314 20-21 -14.592744751365121 2314 22-23 -13.870771279150077 2314 24-25 -15.361683437470226 2314 26-27 -14.715239066956268 2314 28-29 -15.140545239811708 2314 30-31 -16.803268993374946 2314 32-33 -13.184882695332885 2314 34-35 -13.30498827098097 2314 36-37 -13.253632013548911 2314 38-39 -11.24396048652261 2314 40-41 -15.17954928055512 2314 42-43 -15.297778127660482 2314 44-45 -11.672881860833932 2314 46-47 -11.472758739855998 2314 48-49 -10.339964810017221 2314 50-51 -9.655448693577718 2314 52-53 -9.514720746896732 2314 54-55 -10.490347551100896 2314 56-57 -9.59280725481246 2314 58-59 -7.89424295625561 2314 60-61 -8.141250748866788 2314 62-63 -11.049887491866127 2314 64-65 -9.576636704489097 2314 66-67 -11.653982504580197 2314 68-69 -15.749617356945887 2314 70-71 -14.555713769793076 2314 72-73 -12.730386377910172 2314 74-75 -10.795593437846286 2314 76-77 -10.854523178218436 2314 78-79 -10.445372743445192 2314 80-81 -10.226598335494618 2314 82-83 -10.034984767538745 2314 84-85 -10.582016925672642 2314 86-87 -10.782038337304208 2314 88-89 -9.255242066533881 2314 90-91 -8.76243128708778 2314 92-93 -9.96671833349927 2314 94-95 -10.104106425813395 2314 96-97 -9.617324931680233 2314 98-99 -9.46070908565152 2314 100-101 -8.92817447430905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90968.0 3 15583.0 4 10414.0 5 8625.0 6 8844.0 7 10083.0 8 11405.0 9 13291.0 10 14428.0 11 15687.0 12 16817.0 13 18182.0 14 20063.0 15 22152.0 16 24083.0 17 25029.0 18 23472.0 19 21406.0 20 21823.0 21 23530.0 22 27499.0 23 33204.0 24 40040.0 25 48556.0 26 56817.0 27 64561.0 28 72137.0 29 79903.0 30 89623.0 31 99470.0 32 113435.0 33 134854.0 34 161853.0 35 194262.0 36 235632.0 37 276609.0 38 235642.0 39 55757.0 40 55.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.63179636946593 19.726646392092096 14.166359372446344 23.47519786599563 2 14.972741040501905 20.225032985994684 40.7182738913435 24.083952082159914 3 18.16947574384369 25.41187801595701 30.238846142161446 26.17980009803785 4 11.585381938204701 17.25765846565252 39.41119543121683 31.745764164925948 5 12.699790170148667 37.513065479510814 35.4644147591444 14.32272959119612 6 30.29499441522609 37.52257128086522 18.450753307509405 13.731680996399279 7 26.712229396797294 32.158015460750335 22.665588219942304 18.46416692251007 8 25.165911585883364 35.351425953927404 20.890371969932673 18.59229049025656 9 25.43577177564232 16.16043256803983 21.317275883370044 37.08651977294781 10-11 23.954834846587833 26.48629437680232 29.1365230248262 20.422347751783644 12-13 24.934290821203767 24.12531244902229 28.699539392902114 22.240857336871834 14-15 22.17488123362884 25.625626201664936 26.622595770961503 25.576896793744712 16-17 21.549159010644278 28.67370393927655 27.35348063448483 22.423656415594344 18-19 21.439439973621827 27.82179850134481 29.204041912351776 21.534719612681585 20-21 22.42966800796766 27.048391846484638 29.102881916808926 21.419058228738777 22-23 22.133725309546286 26.87773194037677 28.872980721854823 22.115562028222122 24-25 21.92678626429296 27.10145990635773 28.96370298546135 22.00805084388797 26-27 21.743052481897422 27.226360368253765 29.004640484273068 22.025946665575738 28-29 21.723762137972404 27.24466819056434 29.03298396578238 21.998585705680874 30-31 21.95954763923765 27.11747642772615 28.971279210238908 21.951696722797294 32-33 21.866988595499844 27.283571355183394 28.728285215247094 22.12115483406966 34-35 22.188749385617562 27.282280439604893 28.84054547910113 21.68842469567641 36-37 22.06514808232373 26.972694013353905 28.801836213414177 22.160321690908187 38-39 21.958330044530392 27.295769448336195 28.882657977649295 21.86324252948412 40-41 22.08254926987312 27.010759299487546 28.868529896681377 22.038161533957954 42-43 21.958209519582095 27.19192467191925 28.702863437028636 22.147002371470023 44-45 22.077826551923575 27.268016613572133 28.58987015895486 22.064286675549425 46-47 22.139110588401604 27.22352899599503 28.42506227722178 22.212298138381588 48-49 22.08396209976756 27.418757623279316 28.36599631123692 22.131283965716204 50-51 21.942293433242614 27.530366119216026 28.21568784157454 22.31165260596682 52-53 21.98814248333917 27.636211964154178 28.074103828780007 22.301541723726643 54-55 21.935554012168687 27.530354285530734 28.09184190547925 22.442249796821333 56-57 21.943584901191713 27.60490689644875 27.96883607946676 22.482672122892776 58-59 21.973192723003486 27.653369447119218 28.0666525919022 22.306785237975088 60-61 22.119565340308043 27.73294306538667 27.88492715475792 22.26256443954736 62-63 22.06294949408694 27.595560215683264 28.026980114081894 22.314510176147902 64-65 21.95002943598679 27.501853605025712 28.046542523710954 22.50157443527655 66-67 21.954196455036836 27.461784535145416 27.95261011399158 22.631408895826166 68-69 21.9904269408661 27.40568373187552 27.927874853127975 22.676014474130408 70-71 21.973553592791507 27.30963702184996 27.980506561720738 22.736302823637793 72-73 22.079925478098723 27.332935379592854 27.957126095228084 22.630013047080336 74-75 21.940135331641343 27.60832812627012 27.882961367012154 22.568575175076383 76-77 22.02298306014384 27.443884827698895 27.823596740939504 22.70953537121776 78-79 21.973389375291998 27.493581148487927 27.741590627121997 22.791438849098075 80-81 21.958281365337136 27.552945774560573 27.76094776487667 22.727825095225622 82-83 22.126111649835742 27.372737596036444 27.923071491267326 22.578079262860488 84-85 22.090579088379396 27.498097129724435 27.661370378611654 22.749953403284515 86-87 21.979650167460797 27.525275125893238 27.72592838310629 22.769146323539672 88-89 22.137545293239082 27.368221614799936 27.645892879282897 22.848340212678085 90-91 22.109997807696384 27.2659756941679 27.74555237429766 22.878474123838057 92-93 22.203047548355894 27.27677299476064 27.73444716589334 22.785732290990126 94-95 21.927449529804242 27.177175081308192 27.846032956809974 23.04934243207759 96-97 21.931739711978928 27.126206074897958 27.834250351220174 23.107803861902937 98-99 21.916323794212484 27.204045169665413 27.61315201531821 23.266479020803892 100-101 21.94348126319385 27.06454240383218 27.570599155757836 23.421377177216137 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 415.0 1 271.0 2 160.0 3 206.0 4 198.5 5 197.5 6 301.0 7 563.0 8 924.5 9 1187.0 10 1201.5 11 1123.5 12 1195.0 13 1380.5 14 1590.0 15 1762.5 16 1907.5 17 2104.5 18 2343.0 19 2635.5 20 3034.0 21 3605.0 22 4423.0 23 5641.0 24 7291.5 25 9490.5 26 12558.0 27 16337.5 28 20720.0 29 25824.0 30 31630.0 31 37894.5 32 44880.5 33 51756.0 34 58065.5 35 63660.0 36 69714.5 37 75899.5 38 78874.5 39 80023.0 40 81493.5 41 82672.5 42 83216.5 43 84253.0 44 86126.0 45 88494.5 46 89355.0 47 88770.5 48 88861.5 49 88337.0 50 86914.0 51 85147.0 52 81884.5 53 77720.5 54 73772.0 55 68924.0 56 62900.0 57 56104.0 58 49454.0 59 43140.5 60 36395.0 61 29630.0 62 23363.0 63 18110.5 64 13901.0 65 10324.5 66 7762.0 67 6162.5 68 4859.5 69 3789.0 70 2876.0 71 2067.0 72 1496.5 73 1146.0 74 871.0 75 641.0 76 490.5 77 372.0 78 285.0 79 215.0 80 150.5 81 114.5 82 91.5 83 74.5 84 62.0 85 59.5 86 47.0 87 28.0 88 22.5 89 17.0 90 11.5 91 9.0 92 9.0 93 6.5 94 4.5 95 4.0 96 1.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02299044993131603 2 0.21418067373513522 3 0.0 4 0.393711455073787 5 0.2743663873053304 6 0.09820206470662132 7 0.1094099090481379 8 0.1117089540412695 9 0.11072364904421311 10-11 0.1372653024024199 12-13 0.2133801134250269 14-15 0.12199307494804568 16-17 0.11480855934450944 18-19 0.1428692245731782 20-21 0.11119577435530262 22-23 0.09495876909131068 24-25 0.0978941568950412 26-27 0.14830892924442707 28-29 0.11406958059671712 30-31 0.12139778651232411 32-33 0.10444232968797854 34-35 0.14260237113647542 36-37 0.13946171145835815 38-39 0.13539732834550047 40-41 0.11041574123263297 42-43 0.11101102966835455 44-45 0.09202338128758016 46-47 0.09524614971545213 48-49 0.10091165344852644 50-51 0.08701474755254344 52-53 0.09510245940338141 54-55 0.3084209912660923 56-57 0.32788076495795626 58-59 0.3399918055467745 60-61 0.1625958517017449 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2435794.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.34052741229921 #Duplication Level Percentage of deduplicated Percentage of total 1 84.85437050155349 60.5355554481947 2 9.739749782467175 13.896777726900696 3 2.5292595364615393 5.413161278812609 4 1.0196369829599092 2.909657605337819 5 0.5072897444700313 1.8095158960671265 6 0.2926069870890264 1.2524842070072981 7 0.19065947667372912 0.9521223335439757 8 0.144344659471018 0.8238099308648923 9 0.09717941461439529 0.6239547622988513 >10 0.5702825857719428 7.4990522809192734 >50 0.03879102849997325 1.8834308265932675 >100 0.015240196953545778 1.7572556968725215 >500 4.12372109990325E-4 0.17659711674005585 >1k 1.7673090427857544E-4 0.4666248898468 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4986 0.2046971131384674 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3833 0.15736141890488275 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.105437487735005E-5 2 0.0 0.0 0.0 0.0 4.105437487735005E-5 3 0.0 0.0 0.0 4.105437487735005E-5 4.105437487735005E-5 4 0.0 4.105437487735005E-5 0.0 4.105437487735005E-5 4.105437487735005E-5 5 0.0 4.105437487735005E-5 0.0 4.105437487735005E-5 4.105437487735005E-5 6 0.0 4.105437487735005E-5 0.0 4.105437487735005E-5 4.105437487735005E-5 7 0.0 4.105437487735005E-5 0.0 4.105437487735005E-5 4.105437487735005E-5 8 0.0 4.105437487735005E-5 0.0 4.105437487735005E-5 4.105437487735005E-5 9 0.0 4.105437487735005E-5 0.0 4.105437487735005E-5 8.21087497547001E-5 10-11 0.0 4.105437487735005E-5 0.0 4.105437487735005E-5 8.21087497547001E-5 12-13 0.0 4.105437487735005E-5 0.0 4.105437487735005E-5 8.21087497547001E-5 14-15 0.0 1.2316312463205018E-4 0.0 4.105437487735005E-5 1.0263593719337513E-4 16-17 0.0 1.2316312463205018E-4 0.0 8.21087497547001E-5 1.2316312463205018E-4 18-19 0.0 1.2316312463205018E-4 0.0 8.21087497547001E-5 1.6421749950940024E-4 20-21 0.0 1.2316312463205018E-4 0.0 8.21087497547001E-5 2.0527187438675027E-4 22-23 4.105437487735005E-5 1.2316312463205018E-4 0.0 1.8474468694807522E-4 2.0527187438675027E-4 24-25 4.105437487735005E-5 1.2316312463205018E-4 0.0 4.721253110895256E-4 2.873806241414504E-4 26-27 4.105437487735005E-5 1.2316312463205018E-4 0.0 0.0011700496840044766 2.873806241414504E-4 28-29 4.105437487735005E-5 1.2316312463205018E-4 0.0 0.0029559149911692036 3.284349990188004E-4 30-31 8.21087497547001E-5 1.642174995094002E-4 0.0 0.008518782787050136 3.284349990188004E-4 32-33 8.21087497547001E-5 1.642174995094002E-4 0.0 0.022723596494613255 3.694893738961505E-4 34-35 8.21087497547001E-5 2.0527187438675027E-4 0.0 0.04403081705595793 4.1054374877350054E-4 36-37 1.2316312463205018E-4 2.4632624926410035E-4 0.0 0.07919388913840825 4.1054374877350054E-4 38-39 1.2316312463205018E-4 2.4632624926410035E-4 0.0 0.12862335649073772 4.1054374877350054E-4 40-41 1.2316312463205018E-4 2.4632624926410035E-4 0.0 0.18031081446132144 4.1054374877350054E-4 42-43 1.436903120707252E-4 3.0790781158012543E-4 0.0 0.23273725117969746 4.1054374877350054E-4 44-45 1.642174995094002E-4 4.1054374877350054E-4 0.0 0.299655882229778 4.1054374877350054E-4 46-47 1.642174995094002E-4 4.515981236508506E-4 0.0 0.3797324404280493 4.1054374877350054E-4 48-49 1.642174995094002E-4 4.515981236508506E-4 0.0 0.46356547392759817 4.1054374877350054E-4 50-51 1.642174995094002E-4 5.337068734055507E-4 0.0 0.5490817367971184 4.1054374877350054E-4 52-53 1.642174995094002E-4 5.337068734055507E-4 0.0 0.6338590209188462 4.1054374877350054E-4 54-55 1.642174995094002E-4 5.337068734055507E-4 0.0 0.7252665865832661 4.1054374877350054E-4 56-57 1.642174995094002E-4 5.337068734055507E-4 0.0 0.836257089064182 4.721253110895256E-4 58-59 1.642174995094002E-4 5.337068734055507E-4 0.0 0.9611650246285195 5.337068734055507E-4 60-61 1.642174995094002E-4 5.542340608442257E-4 0.0 1.0896241636197477 5.337068734055507E-4 62-63 1.642174995094002E-4 5.747612482829008E-4 0.0 1.2220861041615176 5.337068734055507E-4 64-65 1.642174995094002E-4 6.979243729149509E-4 0.0 1.3629641915531445 5.337068734055507E-4 66-67 1.642174995094002E-4 6.979243729149509E-4 0.0 1.5199971754590085 5.337068734055507E-4 68-69 1.642174995094002E-4 7.18451560353626E-4 0.0 1.6975573468035474 5.337068734055507E-4 70-71 1.642174995094002E-4 7.38978747792301E-4 0.0 1.8870438140499566 5.337068734055507E-4 72-73 1.8474468694807522E-4 7.38978747792301E-4 0.0 2.0813336431570155 5.747612482829008E-4 74-75 2.0527187438675027E-4 7.38978747792301E-4 0.0 2.278394642568296 5.747612482829008E-4 76-77 2.0527187438675027E-4 7.38978747792301E-4 0.0 2.495613340044355 5.747612482829008E-4 78-79 2.0527187438675027E-4 7.800331226696511E-4 0.0 2.7371362274478055 5.747612482829008E-4 80-81 2.0527187438675027E-4 7.800331226696511E-4 0.0 2.9970104204214314 5.952884357215758E-4 82-83 2.0527187438675027E-4 7.800331226696511E-4 0.0 3.263165932751292 6.158156231602509E-4 84-85 2.4632624926410035E-4 7.800331226696511E-4 0.0 3.532893175695482 6.158156231602509E-4 86-87 2.4632624926410035E-4 8.621418724243512E-4 0.0 3.8200685279625453 6.158156231602509E-4 88-89 2.4632624926410035E-4 9.237234347403763E-4 0.0 4.132574429528933 6.363428105989259E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2935 0.0 53.707584 1 GTATCAA 5700 0.0 41.482044 1 ATCAACG 6465 0.0 35.25401 3 TCAACGC 6775 0.0 34.351078 4 CAACGCA 6840 0.0 34.095104 5 AACGCAG 7115 0.0 33.02582 6 TATCAAC 7165 0.0 32.46703 2 ACGCAGA 7740 0.0 29.502884 7 CGCAGAG 7895 0.0 28.622375 8 GTACATG 6465 0.0 25.490616 1 GCAGAGT 8715 0.0 24.400814 9 TACATGG 6635 0.0 23.901598 2 ACATGGG 6590 0.0 22.357449 3 GAGTACT 5280 0.0 21.70722 12-13 CAGAGTA 8605 0.0 21.174011 10-11 AGAGTAC 8010 0.0 20.51969 10-11 GTACTTT 6005 0.0 19.233162 14-15 GTACTAG 810 0.0 18.870993 1 AGTACTT 5895 0.0 18.353493 12-13 GTGGTAT 1420 0.0 17.155825 1 >>END_MODULE