FastQCFastQC Report
Sat 18 Jun 2016
SRR1931117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435794
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67490.2770759760472355No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54720.2246495393288595No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35940.1475494233091961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34900.051.8919221
GTATCAA64850.040.6069151
ATCAACG78200.033.1577263
TCAACGC80100.032.429174
CAACGCA79850.032.351625
TATCAAC83900.030.9616532
AACGCAG83950.030.6019176
ACGCAGA96500.026.3268287
CGCAGAG97400.025.6935258
GTACATG70850.022.6765541
GCAGAGT108000.022.380319
GAGTACT62900.021.55398412-13
TACATGG71600.021.0917642
GTGGTAT17150.021.0644281
CAGAGTA106900.019.87867410-11
GTACTTT68350.019.66165214-15
AGAGTAC97500.019.6278410-11
ACATGGG72900.019.4779153
TGGTATC17700.018.781212
AGTACTT69200.017.6702712-13
Produced by FastQC (version 0.11.5)