FastQCFastQC Report
Sat 18 Jun 2016
SRR1931116_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931116_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811194
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48210.17149296704531955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35860.12756145609303376No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30100.050.8391231
GTATCAA55100.042.653521
ATCAACG65550.034.2713133
CAACGCA68000.033.1428075
TCAACGC68750.033.0956464
TATCAAC70450.032.3449442
AACGCAG71400.031.6802566
ACGCAGA82000.027.2411797
CGCAGAG83250.026.4315818
GTACATG69750.023.5111431
GCAGAGT91650.022.713479
TACATGG71300.022.3314252
ACATGGG71200.021.6584033
AGAGTAC82050.020.47526210-11
GAGTACT53300.020.28218712-13
CAGAGTA92650.019.10730710-11
AGTACTT59550.017.75451512-13
GTATAGG9400.017.7500531
GTACTTT61400.017.48716514-15
GTCCTAC8600.017.1839391
Produced by FastQC (version 0.11.5)