##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931116_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2811194 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.628136656523882 31.0 28.0 31.0 23.0 34.0 2 28.355256165173945 31.0 28.0 33.0 16.0 34.0 3 28.363624851219804 31.0 28.0 33.0 16.0 34.0 4 31.540274346060784 35.0 32.0 37.0 18.0 37.0 5 31.626036125575112 35.0 32.0 37.0 19.0 37.0 6 31.92841511471638 35.0 32.0 37.0 21.0 37.0 7 31.58249484027072 35.0 32.0 37.0 17.0 37.0 8 31.7134758398033 35.0 32.0 37.0 18.0 37.0 9 32.69957569630556 37.0 32.0 39.0 17.0 39.0 10-11 32.677988427692995 37.0 31.5 39.0 17.5 39.0 12-13 32.76931065589923 37.0 32.0 39.0 18.0 39.0 14-15 33.43074526340054 38.0 32.0 40.0 17.0 41.0 16-17 33.3833717986023 37.5 32.0 40.0 17.0 41.0 18-19 33.413094044736866 37.5 32.0 40.0 17.5 41.0 20-21 33.64850024580303 38.0 32.0 40.0 19.0 41.0 22-23 33.53715520878317 38.0 32.0 40.0 18.0 41.0 24-25 33.38856123056608 37.5 31.5 40.0 18.5 41.0 26-27 33.0294807117545 37.0 31.0 40.0 16.5 41.0 28-29 32.98770949283472 37.0 31.0 40.0 17.0 41.0 30-31 32.734617746053814 37.0 30.5 40.0 16.0 41.0 32-33 32.537257122774164 37.0 30.0 40.0 16.0 41.0 34-35 32.513253621059235 37.0 30.0 40.0 16.0 41.0 36-37 32.15958699399614 36.0 30.0 40.0 11.5 41.0 38-39 31.95862736616541 36.0 30.0 40.0 10.0 41.0 40-41 31.81681342518517 36.0 30.0 40.0 9.0 41.0 42-43 32.026226222736675 37.0 30.0 40.0 9.0 41.0 44-45 32.1065047449589 37.0 30.0 40.0 9.0 41.0 46-47 31.880456845027414 36.5 30.0 40.0 8.0 41.0 48-49 31.64813011837675 36.0 30.0 40.0 7.5 41.0 50-51 31.122589013778487 35.5 29.5 39.5 4.0 40.5 52-53 31.0524202527467 36.0 29.0 39.0 2.0 40.5 54-55 31.074593571272562 36.0 29.0 40.0 2.0 41.0 56-57 30.8196687955367 35.5 28.5 40.0 2.0 41.0 58-59 30.661651775010903 35.0 28.0 39.0 2.0 41.0 60-61 30.470914493983695 35.0 28.0 39.0 2.0 41.0 62-63 30.30773703273413 35.0 28.0 39.0 2.0 40.5 64-65 29.97155158982269 34.5 28.0 39.0 2.0 40.0 66-67 29.759344428025955 34.0 28.0 38.0 2.0 40.0 68-69 29.525674322014062 34.0 28.0 38.0 2.0 40.0 70-71 29.193472062049082 34.0 28.0 37.5 2.0 40.0 72-73 28.857761328460434 34.0 27.5 37.0 2.0 40.0 74-75 28.430389009082973 34.0 26.0 36.5 2.0 39.0 76-77 28.111736151969588 33.0 26.0 36.0 2.0 39.0 78-79 27.677352576876586 33.0 26.0 36.0 2.0 38.5 80-81 27.26375180795064 33.0 26.0 35.0 2.0 37.0 82-83 26.930462287554683 33.0 25.5 35.0 2.0 37.0 84-85 26.59175638536508 32.0 25.0 35.0 2.0 36.5 86-87 26.25302825062945 32.0 24.5 35.0 2.0 36.0 88-89 25.90230396763795 32.0 23.5 35.0 2.0 36.0 90-91 25.50594267062323 32.0 21.0 35.0 2.0 35.0 92-93 25.138460917318405 32.0 19.5 35.0 2.0 35.0 94-95 24.822437547888903 32.0 18.0 34.5 2.0 35.0 96-97 24.43318355118857 32.0 9.5 34.0 2.0 35.0 98-99 24.042457404220414 31.5 2.0 34.0 2.0 35.0 100-101 23.442570665702902 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.172008774227145 1101 2 5.789919867236364 1101 3 5.343648761645788 1101 4 6.552619026886028 1101 5 6.238678553960501 1101 6 5.650673624737724 1101 7 6.2895743077251645 1101 8 5.7647176420806225 1101 9 6.386499372734594 1101 10-11 6.357353090023572 1101 12-13 6.192691429984084 1101 14-15 6.59115384780868 1101 16-17 6.548449882508496 1101 18-19 6.6562970900661895 1101 20-21 5.926310194074858 1101 22-23 6.049254982875091 1101 24-25 5.897001104444257 1101 26-27 6.029641725309947 1101 28-29 5.841353723960644 1101 30-31 5.949901517199745 1101 32-33 5.755694007370437 1101 34-35 5.6212742667783075 1101 36-37 5.711949567280282 1101 38-39 5.6448062202535105 1101 40-41 5.740662181097665 1101 42-43 5.650161937957009 1101 44-45 5.588423814044138 1101 46-47 5.47956624146315 1101 48-49 5.297388485203431 1101 50-51 5.306127084694442 1101 52-53 5.078161536445542 1101 54-55 5.362376759034827 1101 56-57 5.243891845870031 1101 58-59 5.167768039437416 1101 60-61 5.088586537678541 1101 62-63 5.02789517728317 1101 64-65 5.134860992712408 1101 66-67 4.96626995225126 1101 68-69 5.088198294282645 1101 70-71 4.963752331927566 1101 72-73 4.749052977452401 1101 74-75 4.956079845395607 1101 76-77 4.745571273215848 1101 78-79 4.660435911818595 1101 80-81 4.611053831810754 1101 82-83 4.422735657162079 1101 84-85 4.433197471823341 1101 86-87 4.292292913894308 1101 88-89 4.418064609324595 1101 90-91 4.347737165118474 1101 92-93 4.107289731565277 1101 94-95 4.052138864799463 1101 96-97 4.121305749847885 1101 98-99 3.7895304934476286 1101 100-101 3.7350670692883554 1102 1 5.319334147349952 1102 2 5.950126636262773 1102 3 5.623868313135553 1102 4 6.9619865135310235 1102 5 6.692482784169766 1102 6 6.144179384270114 1102 7 6.5958973224173185 1102 8 6.204517284765561 1102 9 6.885611716984261 1102 10-11 6.831034787230344 1102 12-13 6.904044790620748 1102 14-15 7.265544027303907 1102 16-17 7.177786269990424 1102 18-19 7.170593394703133 1102 20-21 6.4621456347462995 1102 22-23 6.574259652830079 1102 24-25 6.35867525741407 1102 26-27 6.441559225961857 1102 28-29 6.370867544132693 1102 30-31 6.644586338461661 1102 32-33 6.415757733794848 1102 34-35 6.3433450313555895 1102 36-37 6.3624787874929005 1102 38-39 6.416597462966433 1102 40-41 6.538913115078824 1102 42-43 6.446650754069566 1102 44-45 6.318886851059911 1102 46-47 6.458710496185791 1102 48-49 6.046596660513483 1102 50-51 6.133456429297421 1102 52-53 5.924413778919927 1102 54-55 6.1936753192314775 1102 56-57 6.06704300350097 1102 58-59 6.061553413386498 1102 60-61 5.955328549830309 1102 62-63 5.875292902708878 1102 64-65 6.006846882851512 1102 66-67 5.975831436486818 1102 68-69 5.858808903845382 1102 70-71 5.784516485829592 1102 72-73 5.575275848932598 1102 74-75 5.592965983602564 1102 76-77 5.416692326680501 1102 78-79 5.435485312124694 1102 80-81 5.394409667178959 1102 82-83 5.270537560644794 1102 84-85 5.172258286866068 1102 86-87 5.083142811045022 1102 88-89 5.122733997187723 1102 90-91 5.043827738339253 1102 92-93 5.059908248091407 1102 94-95 4.947724874145987 1102 96-97 4.972177397981042 1102 98-99 4.7637959202594615 1102 100-101 4.674174006905755 1103 1 5.002002712157193 1103 2 5.6234909284156025 1103 3 5.171529848631806 1103 4 6.651572427524663 1103 5 6.331013480143522 1103 6 5.627836968886502 1103 7 6.316402396124904 1103 8 5.828655148016903 1103 9 6.287783680178972 1103 10-11 6.398868605140031 1103 12-13 6.513766136923099 1103 14-15 6.654920068746993 1103 16-17 6.56723818867793 1103 18-19 6.519246601525488 1103 20-21 5.839878167499613 1103 22-23 6.242033550443971 1103 24-25 5.826871587531201 1103 26-27 6.002252548600868 1103 28-29 5.803234244700235 1103 30-31 6.083103122942742 1103 32-33 5.923593325230449 1103 34-35 5.875734247887358 1103 36-37 5.863710447019951 1103 38-39 5.723098029573681 1103 40-41 5.793114244322009 1103 42-43 5.660623267527811 1103 44-45 5.51196965737601 1103 46-47 5.4802151758341004 1103 48-49 5.266487758525788 1103 50-51 5.164253718912814 1103 52-53 5.045519303592727 1103 54-55 5.3047913435126794 1103 56-57 5.385435882794386 1103 58-59 5.424458085211832 1103 60-61 5.241310581795805 1103 62-63 5.207833219821538 1103 64-65 5.207696919312863 1103 66-67 5.221448887662923 1103 68-69 5.220136102512143 1103 70-71 5.114600561245432 1103 72-73 4.919418546317999 1103 74-75 4.996928837165903 1103 76-77 4.915777533396952 1103 78-79 4.8693059411099675 1103 80-81 4.980509386624544 1103 82-83 4.9355526445864335 1103 84-85 4.850957490928529 1103 86-87 4.8266498939645 1103 88-89 4.729996230977914 1103 90-91 4.652583394194362 1103 92-93 4.702207806268937 1103 94-95 4.49648505472506 1103 96-97 4.481596124461042 1103 98-99 4.272491440507618 1103 100-101 4.289782418602044 1104 1 4.5568779023000054 1104 2 5.475904590270154 1104 3 4.999399258021793 1104 4 6.330820930447864 1104 5 6.139655437874726 1104 6 5.63508515636504 1104 7 6.235247533282031 1104 8 5.637630149818367 1104 9 6.338241616764311 1104 10-11 6.281617456215109 1104 12-13 6.272340588072719 1104 14-15 6.37172758604326 1104 16-17 6.227408696484513 1104 18-19 6.467986824065839 1104 20-21 5.863674761535698 1104 22-23 6.049609260920185 1104 24-25 5.6044679332139395 1104 26-27 5.777252487299567 1104 28-29 5.7511128477043485 1104 30-31 5.956792945921176 1104 32-33 5.881692062415617 1104 34-35 5.667633711212204 1104 36-37 5.672653651240072 1104 38-39 5.428129803029993 1104 40-41 5.6588901673990755 1104 42-43 5.752155868235377 1104 44-45 5.64844663644126 1104 46-47 5.757735389248079 1104 48-49 5.460850652456887 1104 50-51 5.281827747755745 1104 52-53 5.071165160324377 1104 54-55 5.291482651722919 1104 56-57 5.2272788658631235 1104 58-59 5.106613179734097 1104 60-61 5.041648446917264 1104 62-63 4.943461800658305 1104 64-65 5.112350566531038 1104 66-67 5.172351721452298 1104 68-69 5.149302936274307 1104 70-71 4.905340460905869 1104 72-73 4.8143454953297855 1104 74-75 4.767976720131188 1104 76-77 4.853925918313156 1104 78-79 4.771570696156665 1104 80-81 4.612299781872434 1104 82-83 4.538959130549504 1104 84-85 4.573075362506007 1104 86-87 4.507119479380194 1104 88-89 4.550215652481825 1104 90-91 4.67527571439031 1104 92-93 4.685868496493182 1104 94-95 4.497872738351145 1104 96-97 4.398103842770222 1104 98-99 4.286084866982556 1104 100-101 4.32337676103716 1105 1 4.190066250790846 1105 2 4.811307423893631 1105 3 4.4820781054693235 1105 4 5.803391920812452 1105 5 3.292181375098174 1105 6 4.521816390071361 1105 7 5.200292834626264 1105 8 0.47049703250731767 1105 9 0.3695665591212851 1105 10-11 1.8969582904171531 1105 12-13 5.085067073397067 1105 14-15 3.260642151114407 1105 16-17 5.4377663828208505 1105 18-19 6.01272543197171 1105 20-21 2.99273266817368 1105 22-23 5.186910933809045 1105 24-25 5.3693515601972095 1105 26-27 5.462057664987302 1105 28-29 2.9173652719638916 1105 30-31 5.2361955980996235 1105 32-33 5.4024585306909785 1105 34-35 5.304128296105368 1105 36-37 5.306013602215682 1105 38-39 5.257181205035135 1105 40-41 5.420807339458587 1105 42-43 2.7214642717074753 1105 44-45 4.760435872985656 1105 46-47 5.077412417110157 1105 48-49 5.047336593035357 1105 50-51 5.098522487048349 1105 52-53 5.18297423565658 1105 54-55 5.311679108752113 1105 56-57 5.307368441504302 1105 58-59 5.235178959545429 1105 60-61 5.013485098469221 1105 62-63 4.7931020773448445 1105 64-65 4.934601631664663 1105 66-67 5.052209869104043 1105 68-69 5.020678101243778 1105 70-71 4.837701266042476 1105 72-73 4.675006845314979 1105 74-75 4.768944712202384 1105 76-77 4.565652145163941 1105 78-79 4.572302552938346 1105 80-81 4.611512378949801 1105 82-83 4.424264480267663 1105 84-85 4.3813008130802125 1105 86-87 4.477219137202276 1105 88-89 4.295508780337233 1105 90-91 4.233543958601615 1105 92-93 4.3187782584065 1105 94-95 4.268613530444252 1105 96-97 4.176493569558524 1105 98-99 4.124349739055258 1105 100-101 4.152286088167127 1106 1 4.063943573323424 1106 2 4.2557589245798475 1106 3 4.382971425678047 1106 4 5.818353505456187 1106 5 6.0212440357011765 1106 6 5.053672969605206 1106 7 5.708921442276878 1106 8 5.167518475250031 1106 9 5.846208634785366 1106 10-11 5.660027038204731 1106 12-13 5.970077381390265 1106 14-15 5.698902681673228 1106 16-17 5.70819714286041 1106 18-19 6.020636655041855 1106 20-21 5.299190834656294 1106 22-23 5.427455275820385 1106 24-25 5.401215195972824 1106 26-27 5.62272623213509 1106 28-29 5.63328127210972 1106 30-31 5.674362339444851 1106 32-33 5.480008097849343 1106 34-35 5.104838859652112 1106 36-37 5.209399833841527 1106 38-39 5.135840013400543 1106 40-41 5.2306378014895465 1106 42-43 2.071221301782355 1106 44-45 4.431621180479031 1106 46-47 4.775076859884617 1106 48-49 4.615822417633648 1106 50-51 4.801852209937977 1106 52-53 4.66352341119353 1106 54-55 4.9087718195367565 1106 56-57 4.855695171371373 1106 58-59 4.738668271103201 1106 60-61 4.520717452278593 1106 62-63 4.52760039619524 1106 64-65 4.606295576585989 1106 66-67 4.744458646316144 1106 68-69 4.778337651690617 1106 70-71 4.620983500424952 1106 72-73 4.33868401754961 1106 74-75 4.312663422180261 1106 76-77 4.279836719984214 1106 78-79 4.370207966369431 1106 80-81 4.3088557627235 1106 82-83 4.299366060748216 1106 84-85 4.350537068991283 1106 86-87 4.369986900996935 1106 88-89 4.471522909285344 1106 90-91 4.396879197120228 1106 92-93 4.412111756385475 1106 94-95 4.348488129267505 1106 96-97 4.373743458128708 1106 98-99 4.3362427993590735 1106 100-101 4.305177206828418 1107 1 2.8320904658429633 1107 2 -0.5390784783351918 1107 3 0.42049007815618467 1107 4 2.0413551033157624 1107 5 2.1824663749250277 1107 6 3.5720550925663517 1107 7 4.57759214699275 1107 8 4.109209502084411 1107 9 4.595786238320638 1107 10-11 2.5237483520160353 1107 12-13 4.685930952249233 1107 14-15 2.7591831190835556 1107 16-17 1.386066577159621 1107 18-19 1.8463951176909958 1107 20-21 1.5522697329639286 1107 22-23 3.8447542323688424 1107 24-25 3.8395847054217427 1107 26-27 2.716066720322573 1107 28-29 3.6147532140125236 1107 30-31 2.903463888171917 1107 32-33 3.3376132287633453 1107 34-35 4.050535828940731 1107 36-37 4.345734375057365 1107 38-39 4.410608387311779 1107 40-41 2.894705110204452 1107 42-43 3.497673247279181 1107 44-45 3.956083729932171 1107 46-47 4.246745919116751 1107 48-49 1.4843632661109432 1107 50-51 3.494639224191612 1107 52-53 3.7401671894220456 1107 54-55 4.257105437058218 1107 56-57 2.6847689495455285 1107 58-59 1.842951980339464 1107 60-61 2.928412177106253 1107 62-63 3.84605421572585 1107 64-65 4.196062797793413 1107 66-67 4.182748105825535 1107 68-69 4.174222924010099 1107 70-71 4.002832392937297 1107 72-73 3.8706559863654277 1107 74-75 3.957577012271127 1107 76-77 3.85370325150647 1107 78-79 3.849194340422624 1107 80-81 3.8969935790882495 1107 82-83 3.72848518906984 1107 84-85 3.720442479900143 1107 86-87 3.5826470114038287 1107 88-89 3.596134288076069 1107 90-91 3.6734900212655575 1107 92-93 3.695658787198848 1107 94-95 3.683190670047228 1107 96-97 3.78803891491372 1107 98-99 3.7468071097041538 1107 100-101 3.832066521306153 1108 1 -2.9023572683244048 1108 2 -2.2562451154634218 1108 3 -3.0452792489557012 1108 4 -5.098994818040456 1108 5 -9.535880843941488 1108 6 -7.929861144312937 1108 7 -6.685838438723053 1108 8 -7.084639003831725 1108 9 -8.74018292280865 1108 10-11 -4.391994159249585 1108 12-13 -3.0150515492779455 1108 14-15 -7.532475654221443 1108 16-17 -6.965584165266545 1108 18-19 -5.334513516770997 1108 20-21 -5.626007544551495 1108 22-23 -4.611251499691733 1108 24-25 -9.139655445050401 1108 26-27 -10.207722394830352 1108 28-29 -7.5174966845146045 1108 30-31 -7.255110679860788 1108 32-33 -3.8930579480961782 1108 34-35 -2.351436481639862 1108 36-37 -5.891652279660729 1108 38-39 -6.532041129299987 1108 40-41 -4.4749665469168 1108 42-43 -5.618084998292922 1108 44-45 -3.025334017107543 1108 46-47 -3.486450794309448 1108 48-49 -2.382129478348009 1108 50-51 -1.9058209834605329 1108 52-53 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38-39 -0.9163053613085772 2313 40-41 -1.6876410904184063 2313 42-43 -2.8796317222456587 2313 44-45 -2.247809849545778 2313 46-47 -5.091848216436194 2313 48-49 -3.9426826469838865 2313 50-51 -4.468388115576538 2313 52-53 -2.709281656599309 2313 54-55 -4.2564168164866665 2313 56-57 -2.931998012147865 2313 58-59 -2.998948024581555 2313 60-61 -1.630935295951172 2313 62-63 -4.474574690331924 2313 64-65 -6.097934615157296 2313 66-67 -6.9467765941681385 2313 68-69 -7.420622661485556 2313 70-71 -6.227302513247018 2313 72-73 -4.711683184232193 2313 74-75 -5.111234632262676 2313 76-77 -4.127435190780542 2313 78-79 -5.207708441358363 2313 80-81 -5.669890081024089 2313 82-83 -5.331060882200557 2313 84-85 -5.040382001459747 2313 86-87 -4.691010781027643 2313 88-89 -6.177709800024207 2313 90-91 -6.011508607879524 2313 92-93 -4.718344671573572 2313 94-95 -4.596328405926254 2313 96-97 -5.594292522656138 2313 98-99 -4.595020837458176 2313 100-101 -4.291127640960887 2314 1 -12.547529835861846 2314 2 -14.56648668974954 2314 3 -13.43407806540383 2314 4 -24.829123711703183 2314 5 -19.122813134235958 2314 6 -16.847042912750204 2314 7 -17.153219830207156 2314 8 -12.896588013823012 2314 9 -15.515075004765562 2314 10-11 -14.760544409538378 2314 12-13 -15.598933761547162 2314 14-15 -16.58509963330662 2314 16-17 -16.49686519803715 2314 18-19 -14.906053552845012 2314 20-21 -15.976639853085635 2314 22-23 -15.541768492983588 2314 24-25 -18.788298616320894 2314 26-27 -15.259713052066433 2314 28-29 -17.295547775854818 2314 30-31 -16.8954624014829 2314 32-33 -15.46004566671888 2314 34-35 -17.276598105375754 2314 36-37 -16.465953598996805 2314 38-39 -15.133093963541924 2314 40-41 -18.48606234571298 2314 42-43 -17.843051052241808 2314 44-45 -14.977979275806852 2314 46-47 -15.573365351592141 2314 48-49 -14.139448145636175 2314 50-51 -13.976763163708998 2314 52-53 -14.147960901882328 2314 54-55 -13.61644762126141 2314 56-57 -12.042221346764734 2314 58-59 -10.967276865551906 2314 60-61 -11.160584891638507 2314 62-63 -13.579695984132462 2314 64-65 -11.727025874302466 2314 66-67 -13.034762732019503 2314 68-69 -15.210957663410854 2314 70-71 -15.733078408510785 2314 72-73 -13.154886880805162 2314 74-75 -11.194503788981969 2314 76-77 -12.67306476336429 2314 78-79 -12.609036897269029 2314 80-81 -12.841819230042187 2314 82-83 -13.209285757056158 2314 84-85 -13.460674646819776 2314 86-87 -14.52793800474732 2314 88-89 -13.215638178922935 2314 90-91 -12.130684580155226 2314 92-93 -13.423677748200449 2314 94-95 -13.367121628875813 2314 96-97 -13.292983319729684 2314 98-99 -13.740765927947203 2314 100-101 -13.378343659443752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 96181.0 3 17397.0 4 11811.0 5 9521.0 6 9880.0 7 11240.0 8 13186.0 9 14965.0 10 16461.0 11 17831.0 12 19550.0 13 20653.0 14 22933.0 15 25018.0 16 26684.0 17 28079.0 18 26565.0 19 24606.0 20 24989.0 21 27028.0 22 30824.0 23 37139.0 24 45820.0 25 55677.0 26 66292.0 27 74764.0 28 83500.0 29 93382.0 30 104124.0 31 115631.0 32 132537.0 33 156018.0 34 186782.0 35 223795.0 36 270501.0 37 321963.0 38 281368.0 39 66445.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.507383373649404 19.79391661955515 14.156794002363107 23.541906004432338 2 14.781890956704643 20.37960549739069 40.87995600718911 23.958547538715564 3 17.96656509653905 25.574933640296614 30.635096688453377 25.823404574710956 4 11.620617959512998 17.459676542000636 39.856687154028315 31.063018344458047 5 12.658753468053982 37.65868218225782 35.69433078320279 13.98823356648541 6 30.092787763124164 37.69857150995528 18.677934599937334 13.530706126983223 7 26.33158345672207 32.440839285548954 22.93648809663706 18.291089161091918 8 24.907350463630497 35.52219012927668 21.19923238074143 18.37122702635139 9 25.517328317506937 16.25042999411006 21.347364732577493 36.88487695580551 10-11 23.77802741885196 26.556379442324634 29.490053797473426 20.17553934134998 12-13 24.759829862304176 24.253403208134745 28.977789795265124 22.008977134295957 14-15 22.063970885837705 25.79122255300562 26.830546009845868 25.31426055131081 16-17 21.59157578063641 28.696577433865066 27.476500026797083 22.23534675870144 18-19 21.42361104928302 27.972944819468903 29.21804580897554 21.385398322272536 20-21 22.343757038302073 27.226346809424722 29.215080540876322 21.21481561139688 22-23 22.046479566786026 27.060438533370316 28.917260588857964 21.975821310985694 24-25 21.89220036285828 27.23420346091529 28.921044053402227 21.952552122824198 26-27 21.734815622084035 27.404890626892037 28.908833187312577 21.95146056371135 28-29 21.740434897726583 27.472774600346035 28.960834703929066 21.82595579799832 30-31 22.070874187152782 27.26971302517881 28.901871413883157 21.757541373785248 32-33 21.87575665644023 27.394577283642548 28.787608495631687 21.94205756428553 34-35 22.131255720657204 27.458923031902454 28.776435247103006 21.63338600033734 36-37 22.09185998817033 27.21372141720203 28.74194279762559 21.952475797002048 38-39 21.965483765316105 27.42636007653177 28.754410345431275 21.853745812720852 40-41 22.15940199192398 27.225067966389023 28.755538043283764 21.859991998403242 42-43 22.012677049675666 27.34938413981754 28.61374170248657 22.02419710802022 44-45 22.059116999435144 27.4212002095643 28.53998019357461 21.97970259742595 46-47 22.07322683141849 27.424207466527665 28.29206348528157 22.21050221677227 48-49 21.989465691393008 27.522301061887504 28.417049663648093 22.0711835830714 50-51 21.912349391960507 27.612335774154474 28.25550564337903 22.219809190505984 52-53 22.008600035676228 27.71964467617772 28.112385810261582 22.15936947788448 54-55 21.897678431537663 27.58965755139084 28.170161171911534 22.342502845159963 56-57 21.93483756142459 27.677387649521968 27.979331287899097 22.40844350115435 58-59 21.940457103043784 27.693936131785478 28.156771524147967 22.20883524102277 60-61 22.21721415884429 27.648654859909822 27.970540710507606 22.163590270738283 62-63 22.037290916244128 27.635250359811526 28.0615460903801 22.265912633564245 64-65 21.867683269102027 27.49925832226449 28.154833853515626 22.478224555117862 66-67 21.952131371936623 27.539685983962688 27.997053920860676 22.511128723240017 68-69 21.91198828682759 27.570758190292093 27.98282509140244 22.53442843147787 70-71 21.953625399029736 27.321309023852496 27.97720470376644 22.74786087335132 72-73 21.999762378548045 27.479924900238117 27.91409984511919 22.60621287609464 74-75 21.898026247921702 27.59074969568091 27.9105959958651 22.600628060532284 76-77 21.916292507738707 27.46377517880303 27.8679806516377 22.751951661820566 78-79 21.93911199298234 27.48890684883363 27.832017285182026 22.739963873002004 80-81 21.974684066627916 27.47037379846428 27.822626257739596 22.732315877168208 82-83 22.027455237881128 27.486221157273388 27.92379323518761 22.562530369657875 84-85 22.056535408086386 27.506710671693234 27.675375658883734 22.76137826133664 86-87 21.837749369129273 27.473646429239672 27.904797747860872 22.78380645377018 88-89 22.018117568549165 27.29342051811437 27.854320975357798 22.834140937978667 90-91 22.042555583143674 27.241574220775938 27.91815506151479 22.797715134565596 92-93 22.08782104685767 27.222614305522846 27.906024984401647 22.783539663217837 94-95 21.83961690299567 27.229177353110458 27.921516622474297 23.009689121419584 96-97 21.84244488285049 27.17663740033594 27.94207728104144 23.03884043577213 98-99 21.828447271870957 27.118797208588237 27.81067404099468 23.242081478546126 100-101 21.931944220142757 27.07931220684165 27.762207090652584 23.22653648236301 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 199.0 1 136.0 2 94.5 3 122.0 4 113.5 5 134.5 6 212.5 7 355.5 8 647.0 9 880.0 10 878.5 11 889.0 12 1048.0 13 1227.0 14 1417.0 15 1641.0 16 1861.0 17 2067.5 18 2315.5 19 2638.5 20 3099.5 21 3672.5 22 4500.5 23 5723.5 24 7432.5 25 10017.0 26 13542.0 27 17906.0 28 23207.0 29 29691.0 30 36861.5 31 44349.5 32 52353.5 33 60336.5 34 68234.5 35 75350.5 36 82760.5 37 89938.0 38 93981.5 39 96241.0 40 98327.0 41 100234.0 42 101379.5 43 102524.5 44 104268.0 45 106465.0 46 106442.0 47 105071.5 48 104575.5 49 103246.5 50 100871.5 51 97596.0 52 92880.5 53 87431.0 54 82155.0 55 75427.5 56 68253.0 57 60634.5 58 53178.5 59 46503.0 60 38789.5 61 31435.5 62 24972.5 63 19159.5 64 14674.0 65 11078.5 66 8210.5 67 6361.5 68 5018.5 69 3865.0 70 3007.5 71 2280.5 72 1719.0 73 1310.5 74 986.0 75 747.5 76 551.0 77 398.5 78 286.5 79 205.5 80 157.5 81 117.5 82 84.5 83 72.5 84 60.5 85 47.0 86 38.0 87 30.0 88 21.5 89 16.5 90 13.0 91 9.5 92 9.0 93 5.5 94 2.5 95 2.5 96 3.0 97 1.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017323599865395275 2 0.18785612092228426 3 0.0 4 0.2730868093770832 5 0.19870560338418478 6 0.09369684198244589 7 0.10657393264214422 8 0.10781895521973937 9 0.10728537411505573 10-11 0.11249668290413255 12-13 0.1484600493598094 14-15 0.1143108586600569 16-17 0.10858375480311923 18-19 0.11486222580156331 20-21 0.10314122753534619 22-23 0.09266525184672421 24-25 0.09460392986040807 26-27 0.12002017648017177 28-29 0.11057579092727148 30-31 0.11799256828237396 32-33 0.09910379717657337 34-35 0.13992275168487128 36-37 0.13679240920406063 38-39 0.1327727648821106 40-41 0.10687629526813162 42-43 0.10844146650853695 44-45 0.08926811881357175 46-47 0.09270082392036978 48-49 0.09787656063580102 50-51 0.08435917265048232 52-53 0.0925229635521419 54-55 0.3068980653772027 56-57 0.32658720814002873 58-59 0.33971330331524613 60-61 0.16395168743245753 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2811194.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.00255888028153 #Duplication Level Percentage of deduplicated Percentage of total 1 86.11183965253517 63.72496484176096 2 8.996238187339028 13.314892923191874 3 2.306566635190822 5.120754996960049 4 0.8843364767724602 2.6177264876933886 5 0.47773641294944846 1.7676858514273026 6 0.2683500833332685 1.1915155705439193 7 0.17571757426141915 0.9102485094906638 8 0.12421652084219227 0.7353872318022441 9 0.09993791861571591 0.6656095536059069 >10 0.5151958932267358 6.874985471976649 >50 0.029031680119958145 1.450083196972949 >100 0.010588511620843571 1.2027270374665207 >500 9.778127723040132E-5 0.04649171585488404 >1k 1.466719157267227E-4 0.3769266112526626 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4821 0.17149296704531955 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3586 0.12756145609303376 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 3.557207364557551E-5 0.0 3.557207364557551E-5 0.0 8 0.0 3.557207364557551E-5 0.0 3.557207364557551E-5 3.557207364557551E-5 9 0.0 7.114414729115102E-5 0.0 3.557207364557551E-5 3.557207364557551E-5 10-11 0.0 7.114414729115102E-5 0.0 3.557207364557551E-5 3.557207364557551E-5 12-13 0.0 7.114414729115102E-5 0.0 3.557207364557551E-5 3.557207364557551E-5 14-15 0.0 7.114414729115102E-5 0.0 3.557207364557551E-5 3.557207364557551E-5 16-17 0.0 7.114414729115102E-5 0.0 3.557207364557551E-5 3.557207364557551E-5 18-19 0.0 7.114414729115102E-5 0.0 7.114414729115102E-5 7.114414729115102E-5 20-21 0.0 8.893018411393878E-5 0.0 1.0671622093672653E-4 1.0671622093672653E-4 22-23 0.0 1.0671622093672653E-4 0.0 1.7786036822787756E-4 1.0671622093672653E-4 24-25 0.0 1.0671622093672653E-4 0.0 3.3793469963296736E-4 1.0671622093672653E-4 26-27 0.0 1.0671622093672653E-4 0.0 0.0011383063566584163 1.0671622093672653E-4 28-29 0.0 1.0671622093672653E-4 0.0 0.002881337965291616 1.4228829458230204E-4 30-31 0.0 1.0671622093672653E-4 0.0 0.008057074680722852 1.7786036822787756E-4 32-33 0.0 1.0671622093672653E-4 0.0 0.02271276902269996 1.7786036822787756E-4 34-35 0.0 1.0671622093672653E-4 0.0 0.04729307191179264 1.7786036822787756E-4 36-37 0.0 1.0671622093672653E-4 0.0 0.08647571103239407 1.7786036822787756E-4 38-39 0.0 1.2450225775951428E-4 0.0 0.14163021121985891 1.9564640505066532E-4 40-41 0.0 1.4228829458230204E-4 0.0 0.20080435572927374 2.4900451551902855E-4 42-43 0.0 1.600743314050898E-4 0.0 0.26225511295200543 2.4900451551902855E-4 44-45 0.0 1.7786036822787756E-4 0.0 0.34300372012746183 2.4900451551902855E-4 46-47 0.0 2.1343244187345306E-4 0.0 0.44016883929035133 2.4900451551902855E-4 48-49 0.0 2.1343244187345306E-4 0.0 0.5380098278525067 2.4900451551902855E-4 50-51 0.0 2.4900451551902855E-4 0.0 0.6359219605619534 2.4900451551902855E-4 52-53 0.0 2.4900451551902855E-4 0.0 0.7367510033103372 2.667905523418163E-4 54-55 0.0 2.845765891646041E-4 0.0 0.8460817716600135 2.845765891646041E-4 56-57 0.0 2.845765891646041E-4 0.0 0.9768447143811491 3.201486628101796E-4 58-59 0.0 2.845765891646041E-4 0.0 1.1208760405720843 3.557207364557551E-4 60-61 0.0 2.845765891646041E-4 0.0 1.2710435494668813 3.557207364557551E-4 62-63 0.0 2.845765891646041E-4 0.0 1.4233809548540584 3.557207364557551E-4 64-65 0.0 4.268648837469061E-4 0.0 1.5850382435363763 3.557207364557551E-4 66-67 0.0 4.268648837469061E-4 0.0 1.7649262199620517 3.557207364557551E-4 68-69 0.0 4.268648837469061E-4 0.0 1.9679716163309968 3.557207364557551E-4 70-71 0.0 4.268648837469061E-4 0.0 2.180888263136589 3.557207364557551E-4 72-73 0.0 4.268648837469061E-4 0.0 2.4025734260958154 3.557207364557551E-4 74-75 0.0 4.4465092056969387E-4 0.0 2.6326713844722205 3.557207364557551E-4 76-77 0.0 4.8022299421526934E-4 0.0 2.8839702987413887 3.557207364557551E-4 78-79 0.0 4.980090310380571E-4 0.0 3.160774389814435 3.557207364557551E-4 80-81 0.0 4.980090310380571E-4 0.0 3.456111531256825 3.735067732785429E-4 82-83 0.0 4.980090310380571E-4 0.0 3.7597903239691037 3.912928101013306E-4 84-85 0.0 4.980090310380571E-4 0.0 4.068787141691395 3.912928101013306E-4 86-87 0.0 4.980090310380571E-4 0.0 4.393240025412689 4.0907884692411835E-4 88-89 0.0 4.980090310380571E-4 0.0 4.743429304416558 4.268648837469061E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3010 0.0 50.839123 1 GTATCAA 5510 0.0 42.65352 1 ATCAACG 6555 0.0 34.271313 3 CAACGCA 6800 0.0 33.142807 5 TCAACGC 6875 0.0 33.095646 4 TATCAAC 7045 0.0 32.344944 2 AACGCAG 7140 0.0 31.680256 6 ACGCAGA 8200 0.0 27.241179 7 CGCAGAG 8325 0.0 26.431581 8 GTACATG 6975 0.0 23.511143 1 GCAGAGT 9165 0.0 22.71347 9 TACATGG 7130 0.0 22.331425 2 ACATGGG 7120 0.0 21.658403 3 AGAGTAC 8205 0.0 20.475262 10-11 GAGTACT 5330 0.0 20.282187 12-13 CAGAGTA 9265 0.0 19.107307 10-11 AGTACTT 5955 0.0 17.754515 12-13 GTATAGG 940 0.0 17.750053 1 GTACTTT 6140 0.0 17.487165 14-15 GTCCTAC 860 0.0 17.183939 1 >>END_MODULE