FastQCFastQC Report
Sat 18 Jun 2016
SRR1931116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2811194
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53580.19059517059299358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43870.15605468708313974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36500.051.4392431
GTATCAA65700.039.2124751
ATCAACG76200.032.9071733
TCAACGC78200.032.1847274
CAACGCA78150.032.1445545
TATCAAC81900.030.9648512
AACGCAG82900.030.359486
ACGCAGA95450.026.1189927
CGCAGAG95750.025.4916138
GTACATG77250.023.1355421
TACATGG78700.021.7843252
GCAGAGT110450.021.4109869
ACATGGG78050.021.235583
GAGTACT62700.020.48673412-13
AGAGTAC97300.019.76583510-11
CAGAGTA109000.018.95115910-11
GTGGTAT19350.018.9147281
GTACTTT69500.018.37978714-15
CATGGGG51150.018.2894784
TACACCG4002.3646862E-1117.807875
Produced by FastQC (version 0.11.5)