##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931116_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2811194 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.354699106500654 31.0 31.0 34.0 30.0 34.0 2 31.375230240246673 31.0 31.0 34.0 30.0 34.0 3 31.540293199259818 31.0 31.0 34.0 30.0 34.0 4 35.175124520043795 37.0 35.0 37.0 33.0 37.0 5 35.05872984930958 37.0 35.0 37.0 32.0 37.0 6 35.17806597481355 37.0 35.0 37.0 32.0 37.0 7 35.022564077754865 36.0 35.0 37.0 32.0 37.0 8 35.070540489201385 37.0 35.0 37.0 32.0 37.0 9 36.524130316157475 38.0 35.0 39.0 32.0 39.0 10-11 36.371223401871234 38.0 35.0 39.0 32.0 39.0 12-13 36.30394380466094 38.0 35.0 39.0 32.0 39.0 14-15 37.29109357091684 39.0 36.0 41.0 32.0 41.0 16-17 37.310316541654544 39.0 36.0 41.0 32.0 41.0 18-19 37.37342211174327 39.0 36.0 41.0 32.0 41.0 20-21 37.34536535009679 39.0 36.0 41.0 32.0 41.0 22-23 37.232253803899695 39.0 36.0 40.0 31.0 41.0 24-25 37.092404508546906 39.0 36.0 40.0 31.0 41.0 26-27 36.875183640830194 39.0 36.0 40.0 30.5 41.0 28-29 36.590918485170356 38.5 35.0 40.0 30.0 41.0 30-31 36.38032095970609 38.0 35.0 40.0 30.0 41.0 32-33 36.27496750491073 38.0 35.0 40.0 30.0 41.0 34-35 36.05448289943703 38.0 35.0 40.0 29.5 41.0 36-37 36.31049262341909 38.0 35.0 40.0 30.0 41.0 38-39 36.311930090915105 38.0 35.0 40.0 30.0 41.0 40-41 36.16551365718624 38.0 35.0 40.0 30.0 41.0 42-43 36.005084494346534 38.0 35.0 40.0 29.0 41.0 44-45 35.79806943241911 38.0 35.0 40.0 28.5 41.0 46-47 35.62728506108081 38.0 34.5 40.0 28.0 41.0 48-49 35.40381506932641 38.0 34.0 40.0 27.0 41.0 50-51 35.08081957346238 38.0 34.0 40.0 26.0 41.0 52-53 34.96955297286491 38.0 34.0 40.0 26.0 41.0 54-55 34.73724118648517 38.0 33.0 40.0 26.0 41.0 56-57 34.54034993671728 38.0 33.0 40.0 26.0 41.0 58-59 34.304758405147425 37.0 33.0 40.0 25.0 41.0 60-61 34.03060176565545 37.0 33.0 40.0 24.5 41.0 62-63 33.746793711141954 37.0 32.5 40.0 24.0 41.0 64-65 33.504758120570834 36.0 32.0 39.0 23.0 41.0 66-67 33.2649096789478 36.0 32.0 39.0 23.0 41.0 68-69 32.94561439018438 35.5 31.5 39.0 22.5 40.0 70-71 32.10426690580586 35.0 31.0 38.5 19.5 40.0 72-73 32.066551970443875 35.0 31.0 38.0 21.0 40.0 74-75 31.748809580555452 35.0 31.0 37.0 20.5 39.5 76-77 31.007655999550366 34.0 30.0 36.0 20.0 39.0 78-79 31.0175066181843 34.0 30.0 36.0 19.5 39.0 80-81 30.73941659664897 34.0 30.0 36.0 18.0 38.5 82-83 30.378745650424694 34.0 30.0 35.5 17.5 37.0 84-85 30.027999312747536 34.0 29.5 35.0 14.5 37.0 86-87 29.691200429426072 34.0 29.0 35.0 10.5 36.0 88-89 29.372400659648534 34.0 29.0 35.0 7.0 36.0 90-91 29.07394633739258 34.0 29.0 35.0 3.5 36.0 92-93 28.736762208513532 34.0 29.0 35.0 2.0 35.0 94-95 28.35454703588582 34.0 29.0 35.0 2.0 35.0 96-97 27.99712364212502 33.0 29.0 35.0 2.0 35.0 98-99 27.530624176061842 33.0 27.0 35.0 2.0 35.0 100-101 26.853312862790688 32.5 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7689646998728108 1101 2 0.6501855294476186 1101 3 0.49335463854851014 1101 4 0.6828138104658237 1101 5 0.7472214114452882 1101 6 0.802156982078742 1101 7 0.6952104472545528 1101 8 0.7475026817809649 1101 9 1.0367931894736202 1101 10-11 0.8551227096021776 1101 12-13 0.9815374088927982 1101 14-15 1.2955521655109408 1101 16-17 1.0644447842320588 1101 18-19 0.8950225706151897 1101 20-21 0.8912735553578628 1101 22-23 0.751006668450799 1101 24-25 0.6393834594677088 1101 26-27 0.9618913628335193 1101 28-29 0.8092646379413893 1101 30-31 1.1003696537853003 1101 32-33 1.0442989194905081 1101 34-35 0.8474652757524694 1101 36-37 1.3492306206736302 1101 38-39 1.025751154164979 1101 40-41 1.0676213897270728 1101 42-43 1.173077309948468 1101 44-45 1.0559863259450353 1101 46-47 1.1288753635602404 1101 48-49 0.9605309722063637 1101 50-51 0.8211568004866265 1101 52-53 0.7764846836353385 1101 54-55 0.913944821122044 1101 56-57 0.7262308211202964 1101 58-59 0.7580060900243026 1101 60-61 0.8146800330530155 1101 62-63 0.6327706927666199 1101 64-65 0.6035097535860672 1101 66-67 0.388611786316055 1101 68-69 0.2923990801392051 1101 70-71 0.6262093717122426 1101 72-73 0.12344344678376729 1101 74-75 0.008855476899256587 1101 76-77 -0.048039552558680754 1101 78-79 0.12496257520351861 1101 80-81 0.09889118749627102 1101 82-83 -0.09531234693357149 1101 84-85 -0.11065451393150383 1101 86-87 -0.2497583692693226 1101 88-89 -0.3882245978489429 1101 90-91 -0.19610157707890963 1101 92-93 -0.37993088214461324 1101 94-95 -0.40411705554405586 1101 96-97 -0.5067325269320371 1101 98-99 -0.4093515280524578 1101 100-101 -0.7040068902689676 1102 1 0.9235060051373409 1102 2 0.8548189203563865 1102 3 0.6484554719646489 1102 4 0.8028985474539212 1102 5 0.9245724979723988 1102 6 0.8805332216943214 1102 7 0.8993092006847476 1102 8 0.930041661973064 1102 9 1.291879383865762 1102 10-11 1.14958384169379 1102 12-13 1.2827860936156767 1102 14-15 1.7330055668824542 1102 16-17 1.5116528088590115 1102 18-19 1.2910844565476154 1102 20-21 1.2639360228262078 1102 22-23 0.9269650194232284 1102 24-25 0.7068352929631203 1102 26-27 1.066531890118796 1102 28-29 1.1355756113766162 1102 30-31 1.5802465883114465 1102 32-33 1.4919543460884839 1102 34-35 1.602871386184276 1102 36-37 1.9430358677771693 1102 38-39 1.6433227208560055 1102 40-41 1.655558306206693 1102 42-43 1.8218477008545193 1102 44-45 1.6834278518661705 1102 46-47 1.689629144510583 1102 48-49 1.467175442851655 1102 50-51 1.3808301411225372 1102 52-53 1.5764779450369844 1102 54-55 1.3945631786944475 1102 56-57 1.5140140849546952 1102 58-59 1.453194061275731 1102 60-61 1.686956261865575 1102 62-63 1.498216578257633 1102 64-65 1.521991420955544 1102 66-67 1.245738377876691 1102 68-69 1.0958807181676136 1102 70-71 1.5047771513582617 1102 72-73 1.0421312301933199 1102 74-75 0.9599832705376166 1102 76-77 0.9372683338423329 1102 78-79 0.8945444753047198 1102 80-81 0.8595553344548996 1102 82-83 0.7104098848917744 1102 84-85 0.8034899339695407 1102 86-87 0.7688030624673985 1102 88-89 0.7000718155665453 1102 90-91 0.792723140871761 1102 92-93 0.7417377996032073 1102 94-95 0.7807529637466111 1102 96-97 0.7529180143199845 1102 98-99 0.8123729922969858 1102 100-101 0.7266912336398761 1103 1 0.7090558597763952 1103 2 0.5037564016935931 1103 3 0.37023335355252485 1103 4 0.6133309947167476 1103 5 0.7170233939718074 1103 6 0.6219356779878495 1103 7 0.6081190910263814 1103 8 0.6594463429889998 1103 9 0.9752153334953917 1103 10-11 0.7287988674775008 1103 12-13 0.8726229368065219 1103 14-15 1.3432645730388728 1103 16-17 1.0644122545093566 1103 18-19 0.8584363163953626 1103 20-21 0.8167820714060596 1103 22-23 0.5963509595971104 1103 24-25 0.4691092346958641 1103 26-27 0.8137065193838922 1103 28-29 0.6455606859279044 1103 30-31 1.1234544525273051 1103 32-33 1.0487453136102687 1103 34-35 0.9697674283386846 1103 36-37 1.3365894439498476 1103 38-39 1.0436143000010176 1103 40-41 0.9552176799789933 1103 42-43 1.1376912562508679 1103 44-45 1.0915761548375045 1103 46-47 1.086515557870591 1103 48-49 1.0182915660164937 1103 50-51 0.7910309175744175 1103 52-53 0.42422379478497163 1103 54-55 0.601442605207609 1103 56-57 0.6582041946529316 1103 58-59 0.8155357741867846 1103 60-61 0.9124678182330612 1103 62-63 0.7704955307593124 1103 64-65 0.6375956460461225 1103 66-67 0.5827899620025576 1103 68-69 0.3670329952644522 1103 70-71 0.7892329861169998 1103 72-73 0.34796339523107633 1103 74-75 0.23419192929149446 1103 76-77 0.2788620063739309 1103 78-79 0.3755605509535762 1103 80-81 0.23563046975303692 1103 82-83 0.2062167002313231 1103 84-85 0.07958029256348453 1103 86-87 -0.011485225606797655 1103 88-89 -0.09587543447744551 1103 90-91 0.03221628566376111 1103 92-93 -0.22069207923682654 1103 94-95 -0.22893856993584905 1103 96-97 -0.3525092301881152 1103 98-99 -0.4293314378854447 1103 100-101 -0.494272333201458 1104 1 0.43817459771931766 1104 2 0.32717759047860184 1104 3 0.17097345412305387 1104 4 0.5706215829253338 1104 5 0.4548551833427297 1104 6 0.5521194213399241 1104 7 0.5500169209680124 1104 8 0.44474824322669804 1104 9 0.7730988722434731 1104 10-11 0.47365426402141253 1104 12-13 0.6713743636438849 1104 14-15 1.1042153594792836 1104 16-17 0.8705504493290306 1104 18-19 0.7193293728239709 1104 20-21 0.6008580245959152 1104 22-23 0.3722697851178509 1104 24-25 0.35873542011483295 1104 26-27 0.15393836567407249 1104 28-29 0.4874490912879068 1104 30-31 0.7917120265411199 1104 32-33 0.9076619170179399 1104 34-35 0.7024480095320342 1104 36-37 1.0449168547859102 1104 38-39 0.6034479155972079 1104 40-41 0.8096279254128191 1104 42-43 0.953531510106302 1104 44-45 1.0017006866636038 1104 46-47 0.935005639437712 1104 48-49 0.823187816124566 1104 50-51 0.6812800231487941 1104 52-53 0.4632509161443963 1104 54-55 0.7754526345760766 1104 56-57 0.6867925141357318 1104 58-59 0.6053710840720115 1104 60-61 0.7581567464496999 1104 62-63 -0.09350304989790459 1104 64-65 -0.8073781846235306 1104 66-67 -0.29583224326094637 1104 68-69 0.16878393461800556 1104 70-71 0.6539436704444554 1104 72-73 0.18235403769329395 1104 74-75 0.07729981540014919 1104 76-77 -0.047235834783862174 1104 78-79 -0.12105868194796443 1104 80-81 0.03504209189557628 1104 82-83 -0.15974706191612498 1104 84-85 -0.3067062767313935 1104 86-87 -0.1156801848042548 1104 88-89 -0.0229955757852629 1104 90-91 0.1090396970047891 1104 92-93 0.1688902263108787 1104 94-95 0.15666864245166678 1104 96-97 0.20253317410135807 1104 98-99 0.10167814481401294 1104 100-101 -0.263775225476234 1105 1 -0.40492595534564657 1105 2 -1.9573230676875362 1105 3 -0.46379141195853535 1105 4 0.2758983807288189 1105 5 0.5393563550325169 1105 6 0.10550362774903732 1105 7 -0.15544988058420017 1105 8 -0.2618568690723677 1105 9 0.496022910114128 1105 10-11 0.7736684715089339 1105 12-13 0.9093369414641188 1105 14-15 1.271201266678709 1105 16-17 0.8366955471467179 1105 18-19 0.558078240932268 1105 20-21 0.8674765056272022 1105 22-23 0.2571399235467027 1105 24-25 -0.16926317283038372 1105 26-27 0.8638865169033565 1105 28-29 0.48912189456212474 1105 30-31 1.079500519845631 1105 32-33 1.0779455018922803 1105 34-35 0.5511215779564935 1105 36-37 0.883399118535074 1105 38-39 0.7006083010138227 1105 40-41 1.024279953232103 1105 42-43 1.269215035754243 1105 44-45 1.2480003208635893 1105 46-47 1.2787040196129666 1105 48-49 1.1961763012126951 1105 50-51 0.8079247965907186 1105 52-53 1.055884590628608 1105 54-55 0.08312775897718439 1105 56-57 0.24651317439019493 1105 58-59 -0.5844388094745412 1105 60-61 0.32626495957570967 1105 62-63 0.8207770156277121 1105 64-65 1.1723821367738054 1105 66-67 0.8737516656468003 1105 68-69 0.8537585751086567 1105 70-71 0.342492435505676 1105 72-73 -0.3264362033033521 1105 74-75 -0.17990577721469592 1105 76-77 0.03111546331832571 1105 78-79 -0.4408217250206192 1105 80-81 -0.15148552968542717 1105 82-83 -0.2545589325287061 1105 84-85 -0.35586247179506003 1105 86-87 -0.5382613600544701 1105 88-89 -0.8886222714831327 1105 90-91 -0.8753286432354379 1105 92-93 -0.4489334947156891 1105 94-95 -0.8425075800341659 1105 96-97 -1.0098011503326099 1105 98-99 -1.5960485715959756 1105 100-101 -1.038610084830669 1106 1 0.2911087895888542 1106 2 0.3447787515291978 1106 3 -0.3273066434444303 1106 4 0.34450327940706416 1106 5 0.07951225093412972 1106 6 0.46372844196407215 1106 7 0.43837285015784744 1106 8 0.5794982351189262 1106 9 0.8417564123268448 1106 10-11 0.15180511798298824 1106 12-13 0.25095048051041857 1106 14-15 0.20973757868690512 1106 16-17 -0.139064204285404 1106 18-19 0.40922711469728057 1106 20-21 0.44309795472053537 1106 22-23 0.48254333476918987 1106 24-25 0.3964410224581627 1106 26-27 0.7452907913394213 1106 28-29 0.655187016617468 1106 30-31 0.3664334773164839 1106 32-33 0.3290068782592712 1106 34-35 -1.1002639151160452 1106 36-37 -0.594393418548421 1106 38-39 -0.6526956665933241 1106 40-41 0.5070012751954849 1106 42-43 0.7983634884968325 1106 44-45 0.6464774662845016 1106 46-47 0.15108012670790316 1106 48-49 0.10211013012940384 1106 50-51 0.7508694956478905 1106 52-53 0.20301987407195554 1106 54-55 0.7964864594996968 1106 56-57 0.4854977929591655 1106 58-59 0.7264689054674065 1106 60-61 1.0875281914990822 1106 62-63 0.9107335138971777 1106 64-65 0.8882834364159322 1106 66-67 0.5249486982619302 1106 68-69 0.5281982801107077 1106 70-71 0.7665299347265488 1106 72-73 0.2893329094095236 1106 74-75 0.2960009792544085 1106 76-77 0.4494891524744098 1106 78-79 0.4684061927647605 1106 80-81 0.5816885677918506 1106 82-83 0.41040276971905953 1106 84-85 0.48025778302934796 1106 86-87 0.4602224299941149 1106 88-89 0.4106979184238533 1106 90-91 0.6370365384451482 1106 92-93 0.2634111650617328 1106 94-95 0.2986451864242241 1106 96-97 0.6050585892215103 1106 98-99 0.6477281171684872 1106 100-101 0.48190623214042105 1107 1 0.3737167285674481 1107 2 0.3794739496124109 1107 3 0.09910747836026346 1107 4 -1.3541092725306427 1107 5 0.08465076029936114 1107 6 0.22597353688584576 1107 7 0.46014888382613606 1107 8 0.4118328519821546 1107 9 0.8390990299728855 1107 10-11 0.6772441541924366 1107 12-13 0.7702036586002095 1107 14-15 1.1269774644287267 1107 16-17 0.8659186460391339 1107 18-19 0.6746680436226598 1107 20-21 0.26495062347679976 1107 22-23 0.4670826326282338 1107 24-25 0.45424442914777075 1107 26-27 0.8619409337486843 1107 28-29 0.7904944608330808 1107 30-31 1.1678146852352356 1107 32-33 1.1222883032967346 1107 34-35 0.955141974829111 1107 36-37 1.2395553282048866 1107 38-39 0.9480606388037387 1107 40-41 0.4129567992806358 1107 42-43 0.16516471511763342 1107 44-45 -0.04433008255877979 1107 46-47 0.25112436664577586 1107 48-49 0.641140352101651 1107 50-51 -0.8152497253953754 1107 52-53 0.7110417508667126 1107 54-55 1.0605254999815372 1107 56-57 0.8957579058774314 1107 58-59 0.8264844330883889 1107 60-61 1.1737978313938058 1107 62-63 1.2016637830112131 1107 64-65 1.0903933016847276 1107 66-67 0.9432298074639007 1107 68-69 0.977312819055598 1107 70-71 1.3373035371889799 1107 72-73 0.8844678025541413 1107 74-75 0.6494777766779869 1107 76-77 0.7260115809667695 1107 78-79 0.8114513460605828 1107 80-81 0.9624404200999805 1107 82-83 0.8907961238666537 1107 84-85 0.6898801685910492 1107 86-87 0.6905760271535719 1107 88-89 0.8200281733884616 1107 90-91 1.014720537116876 1107 92-93 1.1520940866770317 1107 94-95 1.0570567276424363 1107 96-97 0.9622014901164668 1107 98-99 0.929629779090984 1107 100-101 0.7374122400204577 1108 1 0.4057994512444232 1108 2 0.40232967166445377 1108 3 0.26505270410362414 1108 4 0.5167647964072657 1108 5 0.4260554259421099 1108 6 0.432079446806064 1108 7 0.4334936181337312 1108 8 0.5393199546608471 1108 9 0.6239798876951639 1108 10-11 0.5374167672121146 1108 12-13 0.7565229982366404 1108 14-15 1.02899718142951 1108 16-17 0.6418831192515881 1108 18-19 0.4955120129525099 1108 20-21 0.4975762861029196 1108 22-23 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1.7363510047836925 2311 80-81 1.600178985136985 2311 82-83 1.6310072556690614 2311 84-85 1.7905958457158881 2311 86-87 1.6108804338241711 2311 88-89 1.8352264918393146 2311 90-91 2.1811572685136333 2311 92-93 2.361564973677517 2311 94-95 2.4411877694506536 2311 96-97 2.3659488306805336 2311 98-99 2.488772065856242 2311 100-101 2.7078457010988046 2312 1 -0.0803017284551295 2312 2 -0.09672020920932312 2312 3 -0.33150571947454566 2312 4 0.1099198308975744 2312 5 -1.1332802361593153 2312 6 -0.060861221335358096 2312 7 0.2896544065880775 2312 8 -2.9100247750306387 2312 9 -5.712878800912229 2312 10-11 -0.7735445190468297 2312 12-13 -5.5509903977584045 2312 14-15 -2.0835626479293907 2312 16-17 -3.167454444609504 2312 18-19 -2.446208181909391 2312 20-21 -3.3242558387255414 2312 22-23 -1.8997411766771677 2312 24-25 -3.140925874308941 2312 26-27 -3.4473913092469886 2312 28-29 -6.018481966795271 2312 30-31 -6.6257461666333555 2312 32-33 -1.5167819561496358 2312 34-35 0.7330256040531538 2312 36-37 1.1691709744202399 2312 38-39 0.9217167618531619 2312 40-41 -2.875841591365358 2312 42-43 -4.465669217543606 2312 44-45 -3.474020746412428 2312 46-47 0.2798213578407811 2312 48-49 -1.0731005084323897 2312 50-51 -6.0650810271941396 2312 52-53 -1.0706609470359183 2312 54-55 0.8907609504630685 2312 56-57 1.2471909013427052 2312 58-59 1.3298518101985195 2312 60-61 1.6212037580555787 2312 62-63 1.4469910989947792 2312 64-65 1.4945443020662736 2312 66-67 1.2681153415260624 2312 68-69 1.205527818875069 2312 70-71 1.589741671423461 2312 72-73 1.1108576171349398 2312 74-75 0.9747791402181001 2312 76-77 1.0496779259317819 2312 78-79 1.019611386616461 2312 80-81 1.0796750577074548 2312 82-83 -0.05670677681536063 2312 84-85 0.5284627036065999 2312 86-87 1.0838230003443385 2312 88-89 1.0974477910741811 2312 90-91 1.468972829607111 2312 92-93 1.7618823393296097 2312 94-95 1.8426644900529183 2312 96-97 1.9984909981861705 2312 98-99 2.0628447593936734 2312 100-101 2.0907839840109936 2313 1 -0.4338890557373176 2313 2 -0.09176270212716986 2313 3 -0.3257099167341835 2313 4 -1.1542878351716794 2313 5 -1.9015493001588553 2313 6 -2.9508421428824434 2313 7 -1.7227899479242694 2313 8 -3.179262215049139 2313 9 -2.1569008856668077 2313 10-11 -1.4907787215096846 2313 12-13 -2.8539764647064345 2313 14-15 -1.9660350419861672 2313 16-17 -5.30521677333703 2313 18-19 -5.005293572439541 2313 20-21 -4.415420491555508 2313 22-23 -2.229220277099209 2313 24-25 -0.23904259975266484 2313 26-27 -4.086158147427021 2313 28-29 -3.937954996800478 2313 30-31 -4.411034077831829 2313 32-33 -7.393538041019838 2313 34-35 -2.029274216725021 2313 36-37 -4.377079820515291 2313 38-39 -1.6036416818075878 2313 40-41 -0.9213841710034458 2313 42-43 -1.1846089180697135 2313 44-45 -3.0102625802564518 2313 46-47 -0.4897629631397322 2313 48-49 0.572112534291783 2313 50-51 1.9493217594162573 2313 52-53 1.2865758570341512 2313 54-55 -3.727684444692162 2313 56-57 -4.000171358008622 2313 58-59 0.398245173209439 2313 60-61 -6.405766236741201 2313 62-63 -0.944529106821264 2313 64-65 -2.14282778557898 2313 66-67 -2.9142645509408425 2313 68-69 -3.8519893806392993 2313 70-71 -4.652525773798246 2313 72-73 -0.26954071783644196 2313 74-75 1.9552381793578313 2313 76-77 -0.30786962566676124 2313 78-79 1.3215640373651425 2313 80-81 1.4236364382936841 2313 82-83 2.0790320796337305 2313 84-85 1.8645903558006225 2313 86-87 1.6774158199084255 2313 88-89 -1.5300020557200575 2313 90-91 1.857819161340938 2313 92-93 2.678428561217796 2313 94-95 2.793168912785184 2313 96-97 2.916193489947748 2313 98-99 2.8500621959433623 2313 100-101 2.135134160342151 2314 1 -28.852256402676094 2314 2 -14.978555154957359 2314 3 -4.909075338374539 2314 4 -4.431530807832445 2314 5 -1.279292850409142 2314 6 -1.3703646688739752 2314 7 -0.7915231015630795 2314 8 -4.871406997490418 2314 9 -2.726789218358377 2314 10-11 -0.7280717519293987 2314 12-13 0.4164316985588741 2314 14-15 -1.2300897204613293 2314 16-17 -0.4825367579346391 2314 18-19 -3.8988245697441286 2314 20-21 -3.031034661751889 2314 22-23 -5.519362749182381 2314 24-25 -0.7540599274384476 2314 26-27 -4.955141589860595 2314 28-29 -2.0373966602583167 2314 30-31 -4.238719869129483 2314 32-33 -2.268104848875062 2314 34-35 -0.8861282791046818 2314 36-37 -8.555747514007784 2314 38-39 -10.499290507375555 2314 40-41 -7.750995260722355 2314 42-43 -5.36452805553602 2314 44-45 -6.512765468204083 2314 46-47 -7.201738319319944 2314 48-49 -7.3185305153809175 2314 50-51 -2.676785287175967 2314 52-53 -3.2434587983374286 2314 54-55 -6.572505391938989 2314 56-57 -3.092104357623562 2314 58-59 -2.245285824095138 2314 60-61 -0.015989571358069554 2314 62-63 0.43533227169240973 2314 64-65 -1.34382894075803 2314 66-67 -0.3651694411988373 2314 68-69 0.6918111584442528 2314 70-71 0.9447980613962059 2314 72-73 0.6429544689175088 2314 74-75 0.4167322109327287 2314 76-77 -1.0100259598985701 2314 78-79 -1.3725060434973884 2314 80-81 -1.2766908624379312 2314 82-83 -1.8608986096231028 2314 84-85 -0.07052708740307523 2314 86-87 0.5309006100508995 2314 88-89 0.6501096115883733 2314 90-91 0.9237606322689338 2314 92-93 0.9271771172439784 2314 94-95 1.7778627903362043 2314 96-97 1.897710625103695 2314 98-99 2.1424187612109797 2314 100-101 1.8075446339655592 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 20.0 4 56.0 5 91.0 6 230.0 7 619.0 8 1545.0 9 2791.0 10 4224.0 11 6266.0 12 8069.0 13 10211.0 14 12509.0 15 14774.0 16 16942.0 17 18813.0 18 17498.0 19 15517.0 20 15496.0 21 16793.0 22 18352.0 23 20885.0 24 25444.0 25 31423.0 26 39560.0 27 50706.0 28 63783.0 29 80352.0 30 99389.0 31 122392.0 32 149279.0 33 181539.0 34 224007.0 35 275915.0 36 348874.0 37 437020.0 38 392714.0 39 87045.0 40 46.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.39661525357824 21.0338474642176 15.077354644910704 23.492182637293457 2 14.878695671661223 21.532665479507994 40.412721427265424 23.175917421565355 3 18.149375169641026 26.188305361314484 30.967032990488008 24.695286478556486 4 12.723269232137394 17.84122681578554 38.931480730511744 30.504023221565323 5 12.33259283896293 38.46189418341376 36.20195753747974 13.003555440143568 6 30.032114468087222 38.80280051821397 18.115078504009325 13.050006509689476 7 26.215337682137914 33.051756655712836 22.962378263470967 17.770527398678286 8 24.689509155184595 36.564570072360716 20.731831385525155 18.014089386929538 9 25.252615080994055 16.385208562624992 21.130345326576535 37.23183102980442 10-11 23.765506756203948 27.074776767451837 29.368784224781358 19.79093225156286 12-13 24.588591182252095 24.539412790437087 29.1420833994381 21.72991262787271 14-15 21.84673131772478 26.268268927722527 26.72138600181987 25.16361375273282 16-17 21.476159240522 29.249493275810917 27.39097693008736 21.883370553579727 18-19 21.441743259269906 28.364228865030306 29.080899432767715 21.113128442932076 20-21 22.2085882368844 27.519178683506013 29.09230028237112 21.179932797238468 22-23 22.041132700197853 27.396775178091588 28.74942106450142 21.812671057209144 24-25 21.890634175481505 27.56839655745051 28.871654042953416 21.66931522411457 26-27 21.87562145691736 27.685378708508026 28.741237346292248 21.69776248828237 28-29 21.767654165338556 27.73490861641091 28.8450264104549 21.65241080779564 30-31 22.170904266568154 27.5050526951235 28.7634819316903 21.56056110661805 32-33 21.97128372008973 27.629966014005614 28.603941863104104 21.79480840280055 34-35 22.113640527258465 27.652931456755166 28.718707146020574 21.5147208699658 36-37 22.115777111265615 27.473492623930085 28.681076064381443 21.72965420042285 38-39 21.99172301629678 27.616126956436982 28.718035095008513 21.67411493225772 40-41 22.220074006142752 27.442675832177805 28.644261820386053 21.692988341293393 42-43 22.025934249270094 27.538665260360762 28.516397663231828 21.919002827137316 44-45 22.11890439387523 27.569117463181314 28.454886027174766 21.857092115768694 46-47 22.124703583191707 27.512408228217456 28.231405805641668 22.131482382949166 48-49 22.017200150995862 27.64569197484019 28.264387212599974 22.072720661563974 50-51 22.09509297194242 27.685104996746805 28.159813299558262 22.059988731752515 52-53 22.15964824504035 27.67281424421898 28.105838102862464 22.0616994078782 54-55 22.040637747780785 27.582223213858377 28.204943436706063 22.172195601654778 56-57 22.099850897929556 27.70995191845079 27.990174168221927 22.200023015397726 58-59 22.113891762859456 27.687106155609186 28.09128216675622 22.10771991477514 60-61 22.26573623594611 27.695106487692804 27.981346800424532 22.057810475936552 62-63 22.135475075215016 27.682523702846655 28.000690119463595 22.181311102474734 64-65 21.97999407666714 27.55408154099151 28.02935340507148 22.436570977269877 66-67 21.992422024438632 27.596876032757972 27.953576628729355 22.457125314074037 68-69 22.07164343271498 27.590839367710192 27.92364313674711 22.413874062827716 70-71 22.084307617219114 27.34494256394166 27.948758448388656 22.62199137045057 72-73 22.110225716369328 27.50696359011757 27.81321925970723 22.569591433805876 74-75 21.97733320052932 27.651432149006105 27.776896370181703 22.594338280282873 76-77 21.938027044736153 27.590589621349505 27.710912160455663 22.76047117345868 78-79 21.95371432921385 27.665895701257188 27.607628644625738 22.772761324903225 80-81 21.9645391344962 27.651965800169215 27.596651019045893 22.78684404628869 82-83 22.025968103424205 27.655188862895454 27.671534315044628 22.64730871863572 84-85 22.028433469906382 27.681778632139935 27.462227793599446 22.827560104354237 86-87 21.835025171247008 27.74573269814709 27.591133716380234 22.828108414225667 88-89 21.972577477687874 27.5915140905633 27.531715084237092 22.904193347511733 90-91 21.980366056335622 27.48246982020183 27.60928615741874 22.927877966043813 92-93 22.00163637010423 27.494735157055956 27.544342072498313 22.9592864003415 94-95 21.791418764118905 27.5618306053159 27.501019593809477 23.145731036755713 96-97 21.72345236862915 27.482558054740657 27.541074344857662 23.25291523177253 98-99 21.663369076788666 27.53386165916794 27.408983673550697 23.393785590492698 100-101 21.676445132508203 27.51974398715952 27.374089727577267 23.429721152755015 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 217.0 1 158.0 2 109.5 3 130.5 4 130.5 5 133.0 6 207.5 7 406.5 8 649.0 9 838.0 10 890.0 11 893.5 12 981.5 13 1123.5 14 1293.5 15 1495.5 16 1687.0 17 1903.5 18 2159.0 19 2421.5 20 2789.0 21 3322.5 22 4204.0 23 5658.0 24 7472.5 25 9912.5 26 13681.5 27 18309.0 28 23808.0 29 30518.5 30 37635.0 31 45050.0 32 53329.5 33 61514.5 34 69474.0 35 76245.5 36 83543.5 37 91088.5 38 94640.0 39 96514.0 40 98627.0 41 100284.0 42 100986.5 43 102264.5 44 103983.5 45 105834.0 46 106191.5 47 104971.5 48 104410.0 49 103727.0 50 102050.0 51 98055.0 52 92823.0 53 87287.0 54 81612.0 55 75382.0 56 68548.0 57 61042.5 58 53735.5 59 46201.5 60 37875.5 61 30437.0 62 24022.0 63 18363.0 64 13706.5 65 10074.0 66 7421.0 67 5851.5 68 4621.0 69 3563.0 70 2740.5 71 2032.0 72 1524.5 73 1165.5 74 881.5 75 664.5 76 487.5 77 350.5 78 255.0 79 175.5 80 123.5 81 103.5 82 86.5 83 68.5 84 46.5 85 28.0 86 23.5 87 19.0 88 12.0 89 8.5 90 6.0 91 4.5 92 1.5 93 1.0 94 0.5 95 1.0 96 1.0 97 1.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10066896841697869 2 0.0 3 0.003948500174658882 4 4.980090310380572E-4 5 3.201486628101796E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.019368994100015868 26-27 0.009213167074204057 28-29 0.006616405698077045 30-31 0.0025078311920130737 32-33 0.0012805946512407184 34-35 0.0017430316086332 36-37 0.0015118131299369593 38-39 8.893018411393877E-4 40-41 0.002703477597063739 42-43 0.0016541014245192612 44-45 6.936554360887224E-4 46-47 0.03422033484704364 48-49 0.01853305036934484 50-51 0.08595991596453322 52-53 0.001156092393481204 54-55 0.036408017376246535 56-57 0.001156092393481204 58-59 0.0015118131299369593 60-61 0.003361560959506886 62-63 0.0030769843703422816 64-65 0.009835678363001627 66-67 0.005442527267773053 68-69 0.0027746217443548895 70-71 0.005513671415064204 72-73 0.0051223786049628735 74-75 0.0012094505039495673 76-77 0.0 78-79 0.0 80-81 3.735067732785429E-4 82-83 5.157950678608449E-4 84-85 0.0 86-87 0.0017786036822787755 88-89 0.003930714137836094 90-91 0.0014940270931141715 92-93 0.0033437749226840983 94-95 9.070878779621755E-4 96-97 3.9129281010133064E-4 98-99 0.0017786036822787755 100-101 0.0030236262598739186 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2811194.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.73382949483499 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9343794558816 57.003974967805114 2 10.872313918426912 14.945915421669534 3 2.82872851775594 5.832880308798423 4 1.1793902006772141 3.2425601984490746 5 0.6008358609199508 2.0648874809427142 6 0.3462850592771945 1.428089893258051 7 0.24066818103719584 1.1579432004169887 8 0.16255592574969366 0.8938473027083602 9 0.11720341353090526 0.725025549766129 >10 0.6555532825939726 8.171270066922688 >50 0.044093890982505206 2.058087357130361 >100 0.01752447681333186 1.8918727327310025 >500 2.598979743792301E-4 0.0991082683895167 >1k 1.5593878442575007E-4 0.2931089520205412 >5k 5.197959480858337E-5 0.19142829899147487 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5358 0.19059517059299358 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4387 0.15605468708313974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.557207364557551E-5 7 0.0 0.0 0.0 0.0 3.557207364557551E-5 8 0.0 0.0 0.0 0.0 7.114414729115102E-5 9 0.0 3.557207364557551E-5 0.0 0.0 1.0671622093672653E-4 10-11 0.0 3.557207364557551E-5 1.7786036822787755E-5 0.0 1.0671622093672653E-4 12-13 0.0 3.557207364557551E-5 3.557207364557551E-5 0.0 1.0671622093672653E-4 14-15 0.0 3.557207364557551E-5 3.557207364557551E-5 0.0 1.0671622093672653E-4 16-17 0.0 3.557207364557551E-5 3.557207364557551E-5 0.0 1.0671622093672653E-4 18-19 0.0 3.557207364557551E-5 3.557207364557551E-5 0.0 1.4228829458230204E-4 20-21 0.0 3.557207364557551E-5 3.557207364557551E-5 1.7786036822787755E-5 2.1343244187345306E-4 22-23 0.0 7.114414729115102E-5 3.557207364557551E-5 7.114414729115102E-5 2.1343244187345306E-4 24-25 0.0 7.114414729115102E-5 3.557207364557551E-5 2.312184786962408E-4 2.1343244187345306E-4 26-27 0.0 7.114414729115102E-5 3.557207364557551E-5 0.001084948246190053 2.1343244187345306E-4 28-29 0.0 7.114414729115102E-5 3.557207364557551E-5 0.0028635519284688285 2.4900451551902855E-4 30-31 0.0 1.4228829458230204E-4 3.557207364557551E-5 0.008377223343533033 2.845765891646041E-4 32-33 0.0 1.4228829458230204E-4 3.557207364557551E-5 0.024295726299928072 2.845765891646041E-4 34-35 0.0 1.600743314050898E-4 3.557207364557551E-5 0.05113485586551479 2.845765891646041E-4 36-37 0.0 1.7786036822787756E-4 3.557207364557551E-5 0.09375020009291425 2.845765891646041E-4 38-39 0.0 2.1343244187345306E-4 3.557207364557551E-5 0.15328006533878488 2.845765891646041E-4 40-41 0.0 2.1343244187345306E-4 3.557207364557551E-5 0.2177544488213905 2.845765891646041E-4 42-43 0.0 2.1343244187345306E-4 3.557207364557551E-5 0.2828869156664392 2.845765891646041E-4 44-45 0.0 2.312184786962408E-4 3.557207364557551E-5 0.368046459973947 3.0236262598739184E-4 46-47 0.0 2.4900451551902855E-4 3.557207364557551E-5 0.4706007482941412 3.201486628101796E-4 48-49 0.0 2.4900451551902855E-4 3.557207364557551E-5 0.5760008025059815 3.201486628101796E-4 50-51 0.0 3.201486628101796E-4 3.557207364557551E-5 0.6808850616499609 3.201486628101796E-4 52-53 0.0 3.201486628101796E-4 3.557207364557551E-5 0.7874234222184595 3.201486628101796E-4 54-55 0.0 3.201486628101796E-4 3.557207364557551E-5 0.9031571638243394 3.201486628101796E-4 56-57 0.0 3.201486628101796E-4 3.557207364557551E-5 1.0399673590652228 3.557207364557551E-4 58-59 0.0 3.201486628101796E-4 3.557207364557551E-5 1.187698180915298 3.912928101013306E-4 60-61 0.0 3.201486628101796E-4 3.557207364557551E-5 1.338114694325614 3.912928101013306E-4 62-63 0.0 3.201486628101796E-4 3.557207364557551E-5 1.4899718767185757 3.912928101013306E-4 64-65 0.0 3.557207364557551E-4 3.557207364557551E-5 1.650864365817514 3.912928101013306E-4 66-67 0.0 3.912928101013306E-4 3.557207364557551E-5 1.8312681373110502 3.912928101013306E-4 68-69 0.0 4.0907884692411835E-4 3.557207364557551E-5 2.0346336823428053 4.0907884692411835E-4 70-71 0.0 4.268648837469061E-4 3.557207364557551E-5 2.2491332864256255 4.268648837469061E-4 72-73 0.0 4.268648837469061E-4 3.557207364557551E-5 2.479586965538487 4.268648837469061E-4 74-75 0.0 4.4465092056969387E-4 3.557207364557551E-5 2.715678818324171 4.4465092056969387E-4 76-77 0.0 4.980090310380571E-4 3.557207364557551E-5 2.975550602341923 4.6243695739248163E-4 78-79 0.0 4.980090310380571E-4 3.557207364557551E-5 3.260305051874755 4.6243695739248163E-4 80-81 0.0 4.980090310380571E-4 3.557207364557551E-5 3.5628099661567294 5.157950678608449E-4 82-83 0.0 4.980090310380571E-4 3.557207364557551E-5 3.871557779363502 5.691531783292081E-4 84-85 0.0 4.980090310380571E-4 3.557207364557551E-5 4.185925980206275 5.691531783292081E-4 86-87 0.0 4.980090310380571E-4 3.557207364557551E-5 4.518702729160634 5.691531783292081E-4 88-89 0.0 4.980090310380571E-4 3.557207364557551E-5 4.877447091876263 5.691531783292081E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3650 0.0 51.439243 1 GTATCAA 6570 0.0 39.212475 1 ATCAACG 7620 0.0 32.907173 3 TCAACGC 7820 0.0 32.184727 4 CAACGCA 7815 0.0 32.144554 5 TATCAAC 8190 0.0 30.964851 2 AACGCAG 8290 0.0 30.35948 6 ACGCAGA 9545 0.0 26.118992 7 CGCAGAG 9575 0.0 25.491613 8 GTACATG 7725 0.0 23.135542 1 TACATGG 7870 0.0 21.784325 2 GCAGAGT 11045 0.0 21.410986 9 ACATGGG 7805 0.0 21.23558 3 GAGTACT 6270 0.0 20.486734 12-13 AGAGTAC 9730 0.0 19.765835 10-11 CAGAGTA 10900 0.0 18.951159 10-11 GTGGTAT 1935 0.0 18.914728 1 GTACTTT 6950 0.0 18.379787 14-15 CATGGGG 5115 0.0 18.289478 4 TACACCG 400 2.3646862E-11 17.80787 5 >>END_MODULE