FastQCFastQC Report
Sat 18 Jun 2016
SRR1931115_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931115_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255620
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56010.24831310238426688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43000.19063494737588776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24650.10928259192594497No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29800.053.2578281
GTATCAA55350.040.6643641
ATCAACG62700.034.0682683
TCAACGC63850.034.060264
CAACGCA64450.033.3595855
AACGCAG67450.032.6326986
TATCAAC69550.031.8778882
ACGCAGA76100.028.428137
CGCAGAG77350.027.9687258
GTACATG53900.024.1805571
GCAGAGT87450.023.5428649
GTGGTAT16200.023.1560971
TACATGG54200.022.8931852
CAACGCG1503.0020637E-522.19795
GAGTACT56400.022.06606312-13
ACATGGG53250.021.8967843
TGGTATC16150.021.4410322
CAGAGTA86250.020.69917710-11
AGAGTAC79600.019.86007310-11
TACCTGG14500.019.5690522
Produced by FastQC (version 0.11.5)