FastQCFastQC Report
Sat 18 Jun 2016
SRR1931115_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931115_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2255620
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73340.32514341954761883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.2584655216747502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38390.17019710766884494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32950.053.1215821
GTATCAA61350.041.090351
ATCAACG75000.032.6718183
TCAACGC76950.031.843174
CAACGCA76950.031.781465
TATCAAC78950.031.217642
AACGCAG80050.030.7873026
ACGCAGA92500.026.4381527
CGCAGAG94000.025.3595478
GTGGTAT17000.024.3415871
GTACCTG18350.024.1060181
TACCTGG17400.024.0169752
TGGTATC17300.023.057812
TAACGCA1252.2610076E-422.7937854
GCAGAGT102950.022.3246489
GTACATG59900.021.5983541
TACATGG60350.020.8522762
AGAGTAC92650.020.4499910-11
GAGTACT66850.020.42217612-13
ACAACGC2651.8624633E-819.7120443
Produced by FastQC (version 0.11.5)