FastQCFastQC Report
Sat 18 Jun 2016
SRR1931114_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931114_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349998
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55280.235234242752547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41410.1762129159258859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25550.10872349678595472No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.055.652071
GTATCAA55100.044.568441
TCAACGC67250.035.067964
CAACGCA67450.034.9968035
ATCAACG66200.034.9639053
AACGCAG71000.033.027536
TATCAAC72550.032.725492
ACGCAGA79650.028.316387
CGCAGAG81000.026.9033958
GTACATG64050.026.4341051
CTAACGC2501.2732926E-1124.8164463
GTGGTAT13950.024.4114271
TACATGG67500.024.0887532
ACATGGG65050.023.8436243
GCAGAGT87850.023.6687629
TGGTATC14600.023.3251192
AGAGTAC79650.020.78702210-11
GAGTACT53350.020.60263312-13
TAACGCC2601.4082616E-820.1973844
CAGAGTA87650.019.78538110-11
Produced by FastQC (version 0.11.5)