##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1931114_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2349998 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.685379306705794 31.0 28.0 31.0 23.0 34.0 2 28.390084161773753 31.0 28.0 33.0 16.0 34.0 3 28.451861661158862 31.0 28.0 34.0 16.0 34.0 4 31.68099887744585 35.0 32.0 37.0 19.0 37.0 5 31.77868023717467 35.0 32.0 37.0 19.0 37.0 6 32.03619279675983 35.0 32.0 37.0 22.0 37.0 7 31.714941033992368 35.0 32.0 37.0 17.0 37.0 8 31.922255252983195 35.0 32.0 37.0 19.0 37.0 9 32.88873692658462 37.0 32.0 39.0 17.0 39.0 10-11 32.90048076636661 37.0 32.0 39.0 18.0 39.0 12-13 32.8680773345339 37.0 32.0 39.0 17.5 39.0 14-15 33.67862334350923 38.0 32.0 40.0 17.5 41.0 16-17 33.70006485111902 38.0 32.0 40.0 18.0 41.0 18-19 33.66725992958292 38.0 32.0 40.0 17.5 41.0 20-21 33.90726183596752 38.0 32.0 40.0 21.0 41.0 22-23 33.80312302393449 38.0 32.0 40.0 19.0 41.0 24-25 33.70386102456258 38.0 32.0 40.0 20.0 41.0 26-27 33.36758669581846 38.0 31.5 40.0 17.5 41.0 28-29 33.27909853540301 37.5 31.0 40.0 17.5 41.0 30-31 32.981735516370655 37.0 31.0 40.0 16.5 41.0 32-33 32.76832214325289 37.0 30.0 40.0 16.0 41.0 34-35 32.73467402950981 37.0 30.0 40.0 16.0 41.0 36-37 32.36060988137011 37.0 30.0 40.0 13.5 41.0 38-39 32.200431021643425 36.5 30.0 40.0 12.5 41.0 40-41 32.09391859056902 36.5 30.0 40.0 9.0 41.0 42-43 32.30852387959479 37.0 30.0 40.0 9.0 41.0 44-45 32.29475280404494 37.0 30.0 40.0 9.0 41.0 46-47 32.04951770171719 37.0 30.0 40.0 8.0 41.0 48-49 31.820791975142107 37.0 30.0 40.0 7.5 41.0 50-51 31.309052390682886 35.5 29.5 39.5 6.5 40.5 52-53 31.23449700808256 36.0 29.0 39.5 2.0 40.5 54-55 31.205882090112418 36.0 29.0 40.0 2.0 41.0 56-57 30.963528905130985 36.0 29.0 40.0 2.0 41.0 58-59 30.782026835767518 35.0 29.0 39.5 2.0 41.0 60-61 30.61803010045115 35.0 29.0 39.0 2.0 41.0 62-63 30.458060815370906 35.0 28.5 39.0 2.0 40.5 64-65 30.10397157784815 35.0 28.0 39.0 2.0 40.0 66-67 29.85805073025594 34.0 28.0 38.5 2.0 40.0 68-69 29.662303329619853 34.0 28.0 38.0 2.0 40.0 70-71 29.380227557640474 34.0 28.0 37.5 2.0 40.0 72-73 29.026948959105496 34.0 28.0 37.0 2.0 40.0 74-75 28.597627955428045 34.0 27.0 36.5 2.0 39.0 76-77 28.30397600338383 34.0 27.0 36.0 2.0 39.0 78-79 27.903247151699702 33.0 26.0 36.0 2.0 38.5 80-81 27.505998302977282 33.0 26.0 35.0 2.0 37.0 82-83 27.179017599163913 33.0 26.0 35.0 2.0 37.0 84-85 26.865114991587227 33.0 26.0 35.0 2.0 36.5 86-87 26.559902816938568 33.0 25.0 35.0 2.0 36.0 88-89 26.209595284761946 32.0 24.5 35.0 2.0 36.0 90-91 25.821661975882535 32.0 23.5 35.0 2.0 35.5 92-93 25.465302310895584 32.0 21.0 35.0 2.0 35.0 94-95 25.166038864713926 32.0 19.5 35.0 2.0 35.0 96-97 24.758677028661303 32.0 17.0 35.0 2.0 35.0 98-99 24.400713532522154 32.0 4.5 34.0 2.0 35.0 100-101 23.800734511263414 31.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 5.31940026665724 1101 2 5.971040918799044 1101 3 5.538016475187135 1101 4 6.734769522233567 1101 5 6.330964614245399 1101 6 5.956308079459227 1101 7 6.48905758000463 1101 8 5.975823502436615 1101 9 6.64643724723517 1101 10-11 6.643965436241299 1101 12-13 6.460505654847129 1101 14-15 6.694461439059655 1101 16-17 6.708895275393623 1101 18-19 6.590327675714374 1101 20-21 6.086659466497242 1101 22-23 6.190193704729992 1101 24-25 6.162674054350205 1101 26-27 6.348803085304432 1101 28-29 6.1016560211229915 1101 30-31 6.239648440266954 1101 32-33 6.1259604901484614 1101 34-35 5.948889903933793 1101 36-37 6.0648495247276735 1101 38-39 6.04447080887936 1101 40-41 6.116059164344083 1101 42-43 5.891998102000514 1101 44-45 5.941653515463887 1101 46-47 5.688814479398808 1101 48-49 5.632738165305177 1101 50-51 5.679767744067586 1101 52-53 5.5002943687404695 1101 54-55 5.785798727686306 1101 56-57 5.740786973876112 1101 58-59 5.6843929719367345 1101 60-61 5.472275610767838 1101 62-63 5.347924443166587 1101 64-65 5.45116453373554 1101 66-67 5.446984300716206 1101 68-69 5.4184463907313365 1101 70-71 5.292044858011195 1101 72-73 5.134362076504299 1101 74-75 5.290070085061952 1101 76-77 5.176727009953815 1101 78-79 5.013359898426575 1101 80-81 4.906612276425982 1101 82-83 4.857055985585589 1101 84-85 4.862257867012669 1101 86-87 4.758679800859081 1101 88-89 4.823219876317712 1101 90-91 4.726760904384957 1101 92-93 4.6328050981358935 1101 94-95 4.594234562250296 1101 96-97 4.422192234217707 1101 98-99 4.200143246492505 1101 100-101 4.137056712815081 1102 1 5.493623265358131 1102 2 6.018027882916769 1102 3 5.7437800374323515 1102 4 7.081496440658508 1102 5 6.5983045775119855 1102 6 6.092924573658038 1102 7 6.710816304190448 1102 8 6.079943468391011 1102 9 6.934654108768115 1102 10-11 6.898134006773926 1102 12-13 6.929707263053903 1102 14-15 7.171607096005406 1102 16-17 7.092343064669251 1102 18-19 7.212891063251892 1102 20-21 6.502918593854684 1102 22-23 6.630979889382594 1102 24-25 6.491197662288201 1102 26-27 6.590134223592301 1102 28-29 6.445705432044463 1102 30-31 6.6482502187017545 1102 32-33 6.416708037521129 1102 34-35 6.392208902276312 1102 36-37 6.636178983133799 1102 38-39 6.348202274184203 1102 40-41 6.574355092310757 1102 42-43 6.42679640420004 1102 44-45 6.3420183857770205 1102 46-47 6.231291521015088 1102 48-49 5.873681698880411 1102 50-51 5.936828958958056 1102 52-53 5.84445218802999 1102 54-55 6.042724656100951 1102 56-57 5.959033289335508 1102 58-59 5.967386409198756 1102 60-61 5.868050260232515 1102 62-63 5.804071466416151 1102 64-65 5.8437721581587745 1102 66-67 5.829122006221741 1102 68-69 5.769331016453684 1102 70-71 5.596353754664872 1102 72-73 5.500562408000963 1102 74-75 5.551410406703312 1102 76-77 5.451802268655605 1102 78-79 5.382507974402024 1102 80-81 5.350925204795821 1102 82-83 5.26436358313072 1102 84-85 5.256251953828958 1102 86-87 5.239977677488568 1102 88-89 5.266782009543533 1102 90-91 5.245339276467561 1102 92-93 5.11798831723727 1102 94-95 5.0713197434535395 1102 96-97 5.094193085357784 1102 98-99 4.846932320541693 1102 100-101 4.805522980789814 1103 1 5.123378310032361 1103 2 5.757480414456442 1103 3 5.336183410587658 1103 4 6.795014661720646 1103 5 6.307773840972818 1103 6 5.627752402189309 1103 7 6.20417473540552 1103 8 5.68805945406849 1103 9 6.370941797420528 1103 10-11 6.386378828244329 1103 12-13 6.488545482108226 1103 14-15 6.669184397481164 1103 16-17 6.609328307114794 1103 18-19 6.565055922871078 1103 20-21 5.893397210459444 1103 22-23 6.153398321266941 1103 24-25 5.870040733599652 1103 26-27 5.998349262243039 1103 28-29 6.018342382819149 1103 30-31 6.175441514756365 1103 32-33 5.921936511904558 1103 34-35 5.742595075016663 1103 36-37 5.874373357861909 1103 38-39 5.709175988336064 1103 40-41 5.847036382676517 1103 42-43 5.704940248526242 1103 44-45 5.514382438917604 1103 46-47 5.587588914181765 1103 48-49 5.260164565393147 1103 50-51 5.203534025383785 1103 52-53 5.052644638587161 1103 54-55 5.3740624568572315 1103 56-57 5.421908084369477 1103 58-59 5.544499232543135 1103 60-61 5.2766712989501805 1103 62-63 5.167523261751288 1103 64-65 5.089153144003571 1103 66-67 5.369637182074232 1103 68-69 5.439327419090638 1103 70-71 5.255634533033842 1103 72-73 5.075747630368191 1103 74-75 5.163375403524245 1103 76-77 5.16262096155792 1103 78-79 5.0711572900664486 1103 80-81 4.992193735398505 1103 82-83 4.967083273255543 1103 84-85 4.94047525804875 1103 86-87 4.93785314179938 1103 88-89 4.882512106923311 1103 90-91 4.987425914355324 1103 92-93 5.055050526520141 1103 94-95 4.938688397872443 1103 96-97 4.824333968133946 1103 98-99 4.606177627237809 1103 100-101 4.575990387007394 1104 1 4.682457039092398 1104 2 5.474145219104347 1104 3 5.096366473083645 1104 4 6.433800517990679 1104 5 6.031351260010791 1104 6 5.584133502090097 1104 7 6.111406218451638 1104 8 5.466274426904132 1104 9 6.155083639044285 1104 10-11 6.201251822385306 1104 12-13 6.130769381884111 1104 14-15 6.227478281520124 1104 16-17 6.1836776467343455 1104 18-19 6.405802531515153 1104 20-21 5.752921743557554 1104 22-23 5.859543624874455 1104 24-25 5.767370829049639 1104 26-27 5.947668758829948 1104 28-29 5.838442384470014 1104 30-31 6.099105683575601 1104 32-33 5.89395684174897 1104 34-35 5.739801525839429 1104 36-37 5.825572149672251 1104 38-39 5.587674657143474 1104 40-41 5.781033521584593 1104 42-43 5.738625155133796 1104 44-45 5.720402337344073 1104 46-47 5.645698707912324 1104 48-49 5.492175722807005 1104 50-51 5.452044616047399 1104 52-53 5.242663324041402 1104 54-55 5.664242394781532 1104 56-57 5.492531494283611 1104 58-59 5.480315939474924 1104 60-61 5.309706418598463 1104 62-63 4.979750297068055 1104 64-65 5.1603026907651675 1104 66-67 5.242586623630398 1104 68-69 5.197388931963605 1104 70-71 4.953946907824051 1104 72-73 4.9697735886099395 1104 74-75 5.107218124311544 1104 76-77 5.095823575141203 1104 78-79 5.128665643510406 1104 80-81 4.994656097527535 1104 82-83 4.80954573630105 1104 84-85 4.750199674336034 1104 86-87 4.670326458302696 1104 88-89 4.747941351060518 1104 90-91 4.762243703890601 1104 92-93 4.696404323302538 1104 94-95 4.510206783401809 1104 96-97 4.500740007568485 1104 98-99 4.429519954752482 1104 100-101 4.389420110936651 1105 1 4.613649268933624 1105 2 5.276513174644382 1105 3 4.949434189878314 1105 4 6.425339281549132 1105 5 3.780105325114782 1105 6 4.93237849857271 1105 7 5.615778111438946 1105 8 1.1671009525450877 1105 9 0.8168229694864664 1105 10-11 2.4038864852397204 1105 12-13 5.510726684474353 1105 14-15 3.653356242763085 1105 16-17 5.890448866717037 1105 18-19 6.338795906417825 1105 20-21 3.4268089725317488 1105 22-23 5.658121933913712 1105 24-25 5.751991990258304 1105 26-27 5.91390555465032 1105 28-29 3.4454226431609882 1105 30-31 5.596849682378014 1105 32-33 5.785827572707216 1105 34-35 5.675292763807267 1105 36-37 5.785673132329318 1105 38-39 5.755597447270503 1105 40-41 5.932767248982298 1105 42-43 3.3698603567435086 1105 44-45 5.4334705229458855 1105 46-47 5.672521002568793 1105 48-49 5.561145943964107 1105 50-51 5.655437361363386 1105 52-53 5.5030764363641325 1105 54-55 5.821664604093797 1105 56-57 5.7918120151603105 1105 58-59 5.61532034386477 1105 60-61 5.471206850513681 1105 62-63 5.3684880111910545 1105 64-65 5.415386817674463 1105 66-67 5.487185552459348 1105 68-69 5.53167905805941 1105 70-71 5.393206915724516 1105 72-73 5.284961887929452 1105 74-75 5.398919751738255 1105 76-77 5.230112848860379 1105 78-79 5.2071800855673125 1105 80-81 5.199231315636062 1105 82-83 5.0901839146763095 1105 84-85 4.964048150806043 1105 86-87 5.113005062504008 1105 88-89 5.10635010640685 1105 90-91 5.09375062052737 1105 92-93 5.16067968730065 1105 94-95 5.036792277176929 1105 96-97 4.980841874566298 1105 98-99 5.083666840288156 1105 100-101 5.126013391971352 1106 1 4.2432044249392895 1106 2 4.420630465519352 1106 3 4.555209718862034 1106 4 6.1089499131119815 1106 5 6.003078692286099 1106 6 5.272779533512995 1106 7 6.040156223530371 1106 8 5.437540315987864 1106 9 6.126383305497313 1106 10-11 6.084694853334778 1106 12-13 6.304808550655199 1106 14-15 5.997589354487662 1106 16-17 6.046290228616421 1106 18-19 6.240251943112778 1106 20-21 5.520573254009339 1106 22-23 5.615427663830367 1106 24-25 5.554616003206547 1106 26-27 5.927800631258712 1106 28-29 6.010051826390857 1106 30-31 6.013356131307667 1106 32-33 5.847665320978411 1106 34-35 5.301770081837493 1106 36-37 5.471164830619653 1106 38-39 5.367597601079531 1106 40-41 5.619436492392158 1106 42-43 2.376108296011928 1106 44-45 4.781970840729475 1106 46-47 5.038214782938347 1106 48-49 4.889895392594106 1106 50-51 4.97190971153881 1106 52-53 4.8064026449804516 1106 54-55 5.0765651674414585 1106 56-57 5.091541059343285 1106 58-59 5.100540754853103 1106 60-61 4.782894767577027 1106 62-63 4.707947960292639 1106 64-65 4.7598442342308545 1106 66-67 4.861768433868168 1106 68-69 4.843272722895392 1106 70-71 4.752293648104764 1106 72-73 4.5226492100877635 1106 74-75 4.6678920731849765 1106 76-77 4.7202469721003055 1106 78-79 4.779235968630019 1106 80-81 4.690221001591617 1106 82-83 4.685015246282386 1106 84-85 4.709465592042594 1106 86-87 4.889606289617177 1106 88-89 4.929998885260407 1106 90-91 4.843850949979235 1106 92-93 4.778360167609115 1106 94-95 4.925006704640502 1106 96-97 4.741127632292329 1106 98-99 4.703951135060503 1106 100-101 4.7279449107117415 1107 1 3.0181709587109964 1107 2 0.013494413710986919 1107 3 1.1411303123617635 1107 4 2.7322865177236615 1107 5 3.012479258530181 1107 6 3.9679996219564444 1107 7 4.959021938651102 1107 8 4.295737154568119 1107 9 4.931925726287169 1107 10-11 2.5995971832170426 1107 12-13 4.910313859236741 1107 14-15 3.4953858892882117 1107 16-17 2.2075771261940034 1107 18-19 2.897851522246011 1107 20-21 2.5549391127176015 1107 22-23 4.296110135863707 1107 24-25 4.229025773024247 1107 26-27 3.484244789798275 1107 28-29 4.125556184650456 1107 30-31 3.599529489661144 1107 32-33 4.064016966888683 1107 34-35 4.617251680867913 1107 36-37 4.7163482398690455 1107 38-39 4.766823752646296 1107 40-41 3.5554164896739806 1107 42-43 4.0651617418643085 1107 44-45 4.401663312851728 1107 46-47 4.587428815879527 1107 48-49 2.3071512761897104 1107 50-51 3.806616392879466 1107 52-53 3.944881353900527 1107 54-55 4.517541918271085 1107 56-57 3.2969382529259406 1107 58-59 2.2582726054070648 1107 60-61 3.20368647236338 1107 62-63 3.8795386341124747 1107 64-65 4.234650021564505 1107 66-67 4.256914609665255 1107 68-69 4.157976166444133 1107 70-71 3.810672110183628 1107 72-73 3.9166433370288374 1107 74-75 4.000094142346477 1107 76-77 4.0225980778397314 1107 78-79 3.920816733929019 1107 80-81 3.984629586682324 1107 82-83 3.7890970926736394 1107 84-85 3.7446167076662924 1107 86-87 3.742734667489202 1107 88-89 3.72890995256202 1107 90-91 3.8291614321688954 1107 92-93 3.9338053564916677 1107 94-95 3.9886483106360977 1107 96-97 4.046230110600337 1107 98-99 4.094578489575511 1107 100-101 4.239427986840383 1108 1 -1.4916320033506842 1108 2 -0.752104838144998 1108 3 -1.377755308183744 1108 4 -3.3341593245876133 1108 5 -8.300566266788103 1108 6 -6.686814090917949 1108 7 -4.885549080065957 1108 8 -5.127907607750927 1108 9 -7.038294968157832 1108 10-11 -2.9223539437963417 1108 12-13 -1.4889061191864847 1108 14-15 -6.150086684211505 1108 16-17 -5.3765750066753135 1108 18-19 -3.549373548198332 1108 20-21 -4.020225784633677 1108 22-23 -3.010114919375873 1108 24-25 -7.559117853569255 1108 26-27 -8.34442869291103 1108 28-29 -5.392815969460081 1108 30-31 -5.087569954526295 1108 32-33 -1.888527584363846 1108 34-35 -0.36346431644277644 1108 36-37 -3.81071649203 1108 38-39 -4.579633355167502 1108 40-41 -2.483982534390261 1108 42-43 -3.192674344899288 1108 44-45 -0.7300460707386272 1108 46-47 -1.533372289628737 1108 48-49 -0.4911024660595764 1108 50-51 0.08753190458162052 1108 52-53 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-8.014702654861086 2313 50-51 -5.0918955308378635 2313 52-53 -5.111432165711506 2313 54-55 -7.482394391518099 2313 56-57 -6.353636778691701 2313 58-59 -5.921984339100565 2313 60-61 -4.238055181944354 2313 62-63 -6.194091798889975 2313 64-65 -6.491397909868436 2313 66-67 -8.416218129832682 2313 68-69 -8.22031184068917 2313 70-71 -7.809513592273905 2313 72-73 -9.22161446274734 2313 74-75 -10.122739253160635 2313 76-77 -9.018912017058682 2313 78-79 -10.410570256890495 2313 80-81 -10.67800336663275 2313 82-83 -10.296163627753632 2313 84-85 -10.729768454334309 2313 86-87 -9.46231824087878 2313 88-89 -10.513745201340821 2313 90-91 -10.710613104484821 2313 92-93 -10.196161342626677 2313 94-95 -10.8133911409002 2313 96-97 -10.511589227567384 2313 98-99 -10.57995543456035 2313 100-101 -9.921364774312227 2314 1 -14.243065549702777 2314 2 -14.932736236684558 2314 3 -14.537072675745941 2314 4 -25.077789273627207 2314 5 -19.50427571041714 2314 6 -17.287850620140414 2314 7 -17.786714149851633 2314 8 -13.096656753092928 2314 9 -15.04764378713443 2314 10-11 -13.789551486027841 2314 12-13 -14.379525848795485 2314 14-15 -17.361668097793043 2314 16-17 -17.763005772540044 2314 18-19 -16.07870085258421 2314 20-21 -17.59196235852627 2314 22-23 -18.164003499321723 2314 24-25 -17.335028805042914 2314 26-27 -16.45079600896584 2314 28-29 -18.088336649904598 2314 30-31 -19.014144576425597 2314 32-33 -16.380974686731363 2314 34-35 -16.87419198365946 2314 36-37 -16.50339742882633 2314 38-39 -15.832498168783683 2314 40-41 -18.50323626981549 2314 42-43 -18.849313784061312 2314 44-45 -15.896403541133276 2314 46-47 -15.173597737013711 2314 48-49 -14.530008777310066 2314 50-51 -14.00326240316819 2314 52-53 -13.424080480851519 2314 54-55 -13.342028943013116 2314 56-57 -12.790869811439684 2314 58-59 -12.251481254145652 2314 60-61 -12.734970227032157 2314 62-63 -14.966991656507439 2314 64-65 -13.000059514044988 2314 66-67 -13.621248979381804 2314 68-69 -16.586590910456614 2314 70-71 -16.527358869919848 2314 72-73 -14.72600459089162 2314 74-75 -13.167471099387498 2314 76-77 -14.052965173204862 2314 78-79 -13.736033000127323 2314 80-81 -13.976176124107832 2314 82-83 -13.698512939571382 2314 84-85 -14.079080272084664 2314 86-87 -14.731658535136019 2314 88-89 -13.929383549703424 2314 90-91 -13.551975230730667 2314 92-93 -13.612986212109355 2314 94-95 -13.31635744370039 2314 96-97 -13.303592075218074 2314 98-99 -13.611398244242363 2314 100-101 -12.93204346439291 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79879.0 3 13146.0 4 9056.0 5 7553.0 6 7725.0 7 8949.0 8 10616.0 9 12211.0 10 13276.0 11 14550.0 12 15733.0 13 16993.0 14 18433.0 15 20673.0 16 22248.0 17 23741.0 18 22073.0 19 19746.0 20 19688.0 21 21212.0 22 24899.0 23 30150.0 24 36605.0 25 44779.0 26 52981.0 27 60363.0 28 66957.0 29 74389.0 30 83638.0 31 93583.0 32 108472.0 33 128994.0 34 155917.0 35 190062.0 36 232801.0 37 280433.0 38 246270.0 39 61141.0 40 63.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.44675687413248 19.891555184658486 14.218192501256036 23.443495439952994 2 14.834370151282137 20.364080141791167 40.989397327637036 23.812152379289657 3 17.939164203544003 25.864617757121493 30.508877028831517 25.68734101050299 4 11.731835687986178 17.39185867120056 39.5002819422613 31.376023698551954 5 12.639873183637585 37.54703676386211 35.56864520042642 14.244444852073885 6 29.807255723932542 37.76287666530083 18.53692050427341 13.892947106493214 7 26.27716611104059 32.502847707903975 22.928798356721313 18.29118782433412 8 25.029776915946456 35.44939713460018 20.980968333378772 18.539857616074592 9 25.354005013855197 16.243740668244236 21.47953456769876 36.92271975020181 10-11 23.80050675924077 26.513090214781265 29.356338188159892 20.330064837818078 12-13 24.79492040256488 24.27071850422038 28.95462667889094 21.979734414323808 14-15 22.172764762881165 25.789181685002287 26.735449748977118 25.30260380313943 16-17 21.435822445319193 28.794464672726843 27.428144858788695 22.34156802316527 18-19 21.31248350747029 27.787658493476954 29.445906420906837 21.453951578145922 20-21 22.22722303967719 27.10150064284053 29.434492036701126 21.23678428078116 22-23 21.97202921720402 26.840569264343113 29.263337793357337 21.924063725095536 24-25 21.789297110379113 27.18855652708777 29.201093538148815 21.8210528243843 26-27 21.630573970631364 27.2805707309421 29.23440174875016 21.85445354967637 28-29 21.585791047751755 27.18110062466413 29.402687161210718 21.8304211663734 30-31 21.80837612791826 27.10463608891048 29.262804022637724 21.82418376053354 32-33 21.709433749271835 27.26891664652114 29.104271454449993 21.91737814975703 34-35 21.95662990518585 27.33266352217675 29.13508059575547 21.575625976881927 36-37 21.877717795415972 26.97915161958769 29.098973453650313 22.044157131346033 38-39 21.754256790988272 27.28521958995218 29.197872765613486 21.76265085344606 40-41 21.91657168927558 27.060196461026326 29.134924409640345 21.888307440057748 42-43 21.798728083422187 27.230720930222652 28.89548413810378 22.07506684825138 44-45 21.85194264029044 27.31965624042299 28.861624907810494 21.966776211476073 46-47 21.976359215216583 27.323199905273306 28.60912332231738 22.09131755719273 48-49 21.847234370530906 27.536689312036934 28.58664393823711 22.02943237919505 50-51 21.80234260459045 27.60896113865787 28.383185460988 22.20551079576368 52-53 21.84090679188828 27.788890841039894 28.208723985369517 22.161478381702313 54-55 21.78575193789847 27.60870947763707 28.286082941755698 22.31945564270876 56-57 21.79275555698809 27.735642486291233 28.139759907461976 22.331842049258693 58-59 21.78247307817688 27.726398319802414 28.292619610858694 22.19850899116201 60-61 21.95548741738282 27.708332534173152 28.16540953442846 22.170770514015565 62-63 21.81495473613169 27.73155551621746 28.24378999471489 22.20969975293596 64-65 21.788720671251635 27.672108657113753 28.19519420867592 22.343976462958693 66-67 21.740167438440373 27.626704363152648 28.147385657349496 22.485742541057483 68-69 21.852103703918047 27.592810717285715 28.08161964393161 22.473465934864624 70-71 21.911635669477167 27.498789360671793 28.09417284610455 22.495402123746487 72-73 22.010125115000097 27.55487451478682 27.978449343361145 22.456551026851937 74-75 21.747869572654956 27.84947051018767 28.002023831509643 22.400636085647733 76-77 21.946827188789097 27.68147036720882 27.94270463208905 22.42899781191303 78-79 22.002146384805435 27.729406578218363 27.774342786674712 22.49410425030149 80-81 22.00497617444781 27.78808747922339 27.741236375520316 22.465699970808487 82-83 22.121678401428426 27.759895965868907 27.801896001613617 22.316529631089047 84-85 22.07578474534872 27.79785344498166 27.648704381876072 22.477657427793556 86-87 21.977125086914967 27.756342771355552 27.805172600146893 22.46135954158259 88-89 22.14640182672496 27.624853297747485 27.73025764277246 22.49848723275509 90-91 22.150465659970774 27.65457672729934 27.75834277305768 22.436614839672202 92-93 22.245869996485105 27.64172565253247 27.748619360527115 22.36378499045531 94-95 22.034188965267205 27.591470290613014 27.725832107091154 22.648508637028627 96-97 22.091040077480915 27.499044680038025 27.798193870803296 22.611721371677763 98-99 22.12616776695129 27.515385119476697 27.60551285575562 22.75293425781639 100-101 22.176274192573782 27.469491463396988 27.594151143958424 22.760083200070806 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 415.0 1 278.5 2 185.0 3 239.0 4 225.5 5 226.5 6 333.5 7 612.5 8 1077.5 9 1380.0 10 1321.5 11 1239.5 12 1359.5 13 1536.5 14 1733.0 15 1949.0 16 2165.5 17 2386.0 18 2606.5 19 2888.5 20 3334.5 21 4058.0 22 4947.0 23 6222.0 24 7975.5 25 10342.0 26 13612.5 27 17570.0 28 21788.5 29 26939.5 30 32673.5 31 38216.0 32 44830.5 33 51430.0 34 57492.5 35 63242.5 36 70002.5 37 75922.5 38 78291.5 39 79466.5 40 80309.5 41 80774.0 42 81072.0 43 81584.5 44 82422.0 45 83747.5 46 82962.0 47 81267.0 48 81435.5 49 81553.0 50 80387.5 51 78315.0 52 75494.5 53 72101.0 54 68821.0 55 64260.0 56 58887.5 57 53208.0 58 47445.0 59 41608.5 60 34860.5 61 28505.5 62 22799.0 63 17625.0 64 13444.5 65 10123.5 66 7489.5 67 5695.0 68 4529.0 69 3516.0 70 2727.0 71 2130.0 72 1573.5 73 1169.5 74 889.5 75 732.5 76 566.5 77 400.5 78 304.0 79 204.5 80 156.0 81 133.5 82 109.5 83 88.0 84 61.0 85 41.0 86 30.5 87 27.0 88 26.5 89 17.5 90 13.5 91 12.5 92 9.5 93 10.0 94 7.0 95 3.0 96 1.5 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05693621866912227 2 0.5548940892715654 3 0.0 4 0.5378727981896154 5 0.2477023384700753 6 0.09463837841564121 7 0.10621285635136711 8 0.10817030482579133 9 0.10651072894530124 10-11 0.16653205662302692 12-13 0.23325551766427036 14-15 0.13617032865559886 16-17 0.12921287592585184 18-19 0.1803831322409636 20-21 0.11080860494349357 22-23 0.1054043450249745 24-25 0.10165966098694551 26-27 0.1742129142237568 28-29 0.1096383911816095 30-31 0.1289575565596226 32-33 0.10619157973751467 34-35 0.15172353338173053 36-37 0.13604266897248424 38-39 0.13195755911281626 40-41 0.10678732492538291 42-43 0.10710647413316947 44-45 0.09585114540523013 46-47 0.09323412190138035 48-49 0.096914976097852 50-51 0.08385113519245549 52-53 0.09193624845638167 54-55 0.3031917473972318 56-57 0.3238087862202436 58-59 0.3358513496607231 60-61 0.16106396686295052 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2349998.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.39637580999548 #Duplication Level Percentage of deduplicated Percentage of total 1 84.9875100049321 58.978251834580185 2 9.248229307679926 12.83587193225541 3 2.516934906856503 5.239984820565295 4 1.0293538582520236 2.85733708754905 5 0.555425534415711 1.9272259560390126 6 0.3502255833343966 1.4582631719609203 7 0.22939610713998076 1.1143480922305267 8 0.16481586562908973 0.9150099000516823 9 0.1304608008870038 0.8148156090144756 >10 0.7239277239076634 9.188543907919005 >50 0.04688561357877846 2.217128182532813 >100 0.016457192178216444 1.8321708441507565 >500 1.887506042974475E-4 0.08732405246789546 >1k 1.2583373619805925E-4 0.2923609276620722 >5k 6.291686809902962E-5 0.24136368102089834 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5528 0.235234242752547 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4141 0.1762129159258859 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2555 0.10872349678595472 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 4.255322770487464E-5 0.0 0.0 0.0 3 0.0 4.255322770487464E-5 0.0 0.0 0.0 4 0.0 4.255322770487464E-5 0.0 0.0 0.0 5 0.0 4.255322770487464E-5 0.0 0.0 0.0 6 0.0 4.255322770487464E-5 0.0 0.0 0.0 7 0.0 4.255322770487464E-5 0.0 0.0 0.0 8 0.0 4.255322770487464E-5 0.0 0.0 8.510645540974928E-5 9 0.0 1.7021291081949856E-4 0.0 4.255322770487464E-5 8.510645540974928E-5 10-11 0.0 1.7021291081949856E-4 0.0 1.063830692621866E-4 8.510645540974928E-5 12-13 0.0 1.7021291081949856E-4 0.0 1.7021291081949856E-4 8.510645540974928E-5 14-15 0.0 2.5531936622924783E-4 0.0 1.7021291081949856E-4 8.510645540974928E-5 16-17 0.0 2.5531936622924783E-4 0.0 2.978725939341225E-4 8.510645540974928E-5 18-19 0.0 2.7659598008168517E-4 0.0 3.404258216389971E-4 1.4893629696706125E-4 20-21 0.0 2.978725939341225E-4 0.0 3.6170243549143446E-4 2.127661385243732E-4 22-23 0.0 3.829790493438718E-4 0.0 4.893621186060584E-4 2.5531936622924783E-4 24-25 0.0 3.829790493438718E-4 0.0 8.510645540974928E-4 2.5531936622924783E-4 26-27 0.0 3.829790493438718E-4 0.0 0.0012765968311462391 2.5531936622924783E-4 28-29 0.0 3.829790493438718E-4 0.0 0.0029787259393412252 2.5531936622924783E-4 30-31 0.0 4.680855047536211E-4 0.0 0.00880851813490905 2.5531936622924783E-4 32-33 0.0 4.680855047536211E-4 0.0 0.022680870366698185 2.5531936622924783E-4 34-35 0.0 6.170218017206824E-4 0.0 0.04500003829790493 2.5531936622924783E-4 36-37 0.0 8.297879402450554E-4 0.0 0.08172347380721176 2.5531936622924783E-4 38-39 0.0 8.936177818023675E-4 0.0 0.13087245180634197 2.5531936622924783E-4 40-41 0.0 0.0010212774649169913 0.0 0.1849363276053852 2.5531936622924783E-4 42-43 0.0 0.001063830692621866 0.0 0.2418299930468026 2.5531936622924783E-4 44-45 0.0 0.0011702137618840526 0.0 0.3132768623632871 2.5531936622924783E-4 46-47 0.0 0.0012340436034413646 0.0 0.3995748081487729 2.5531936622924783E-4 48-49 0.0 0.0012340436034413646 0.0 0.48742594674548656 2.5531936622924783E-4 50-51 0.0 0.0014680863558181752 0.0 0.5793409185880158 2.5531936622924783E-4 52-53 0.0 0.0014893629696706124 0.0 0.6711495073612829 2.5531936622924783E-4 54-55 0.0 0.0014893629696706124 0.0 0.7691070375379043 2.5531936622924783E-4 56-57 0.0 0.0014893629696706124 0.0 0.8862773500232766 2.5531936622924783E-4 58-59 0.0 0.0014893629696706124 0.0 1.0182774623637978 2.5531936622924783E-4 60-61 0.0 0.0015319161973754872 0.0 1.1536180030791514 2.5531936622924783E-4 62-63 0.0 0.0015319161973754872 0.0 1.2895542889823735 2.5531936622924783E-4 64-65 0.0 0.0017446823358998604 0.0 1.4331288792586205 2.5531936622924783E-4 66-67 0.0 0.0017446823358998604 0.0 1.5965971034868964 2.5531936622924783E-4 68-69 0.0 0.001787235563604735 0.0 1.7803632173303976 2.7659598008168517E-4 70-71 0.0 0.001851065405162047 0.0 1.9761718946143785 2.978725939341225E-4 72-73 0.0 0.0018936186328669218 0.0 2.1736401477788494 2.978725939341225E-4 74-75 0.0 0.0019574484744242335 0.0 2.379002024682574 2.978725939341225E-4 76-77 0.0 0.001978725088276671 0.0 2.6020234910838225 2.978725939341225E-4 78-79 0.0 0.0020212783159815452 0.0 2.8499173190785694 2.978725939341225E-4 80-81 0.0 0.0020425549298339826 0.0 3.1237686159732903 3.404258216389972E-4 82-83 0.0 0.0020851081575388574 0.0 3.402875236489563 3.829790493438718E-4 84-85 0.0 0.0020851081575388574 0.0 3.6821095166889504 4.255322770487464E-4 86-87 0.0 0.0020851081575388574 0.0 3.9749821063677504 4.255322770487464E-4 88-89 0.0 0.002170214612948607 0.0 4.291365354353493 4.255322770487464E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2620 0.0 55.65207 1 GTATCAA 5510 0.0 44.56844 1 TCAACGC 6725 0.0 35.06796 4 CAACGCA 6745 0.0 34.996803 5 ATCAACG 6620 0.0 34.963905 3 AACGCAG 7100 0.0 33.02753 6 TATCAAC 7255 0.0 32.72549 2 ACGCAGA 7965 0.0 28.31638 7 CGCAGAG 8100 0.0 26.903395 8 GTACATG 6405 0.0 26.434105 1 CTAACGC 250 1.2732926E-11 24.816446 3 GTGGTAT 1395 0.0 24.411427 1 TACATGG 6750 0.0 24.088753 2 ACATGGG 6505 0.0 23.843624 3 GCAGAGT 8785 0.0 23.668762 9 TGGTATC 1460 0.0 23.325119 2 AGAGTAC 7965 0.0 20.787022 10-11 GAGTACT 5335 0.0 20.602633 12-13 TAACGCC 260 1.4082616E-8 20.197384 4 CAGAGTA 8765 0.0 19.785381 10-11 >>END_MODULE