FastQCFastQC Report
Sat 18 Jun 2016
SRR1931114_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931114_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2349998
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70970.30200025702149536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58740.24995765953843363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39670.1688086543052377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32900.057.6776921
GTATCAA62900.041.58561
ATCAACG79500.032.437253
TCAACGC81200.031.6976344
CAACGCA81750.031.4843775
TATCAAC85050.030.376372
AACGCAG85100.030.355946
ACGCAGA98600.025.8625747
CGCAGAG100050.024.8232678
GTACATG73600.024.3609621
TACATGG76850.022.3705162
ACATGGG72050.022.2130013
GCAGAGT110800.022.2005839
GTGGTAT15400.020.999991
AGAGTAC99400.020.85317610-11
GAGTACT66100.020.29510712-13
TGGTATC15950.019.6514472
CAGAGTA108800.019.37886810-11
GTACTTT75150.018.13540514-15
AGTACTT75250.017.89043612-13
Produced by FastQC (version 0.11.5)