FastQCFastQC Report
Sat 18 Jun 2016
SRR1931113_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1931113_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790026
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54830.19652146610820115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42340.15175485819845408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32400.050.754581
GTATCAA61300.039.9656261
CAACGCA67950.034.9498635
ATCAACG67350.034.8543663
TCAACGC68350.034.7758034
AACGCAG71800.033.26096
TATCAAC75250.032.1761932
ACGCAGA82700.027.7945947
CGCAGAG82550.027.3257548
GCAGAGT91650.023.6264069
GTACATG64850.021.9569841
TACATGG65850.020.8966142
GAGTACT59950.020.52226312-13
ACATGGG63300.020.275813
CAGAGTA94300.019.65652310-11
GTGGTAT17100.019.6258341
AGAGTAC85750.019.23003210-11
GTACTTT67350.018.60400414-15
GTATAAG14550.018.452331
TCTAACG3459.567884E-1018.0665022
Produced by FastQC (version 0.11.5)